Potential chitinase activating factor from yeast cells of Candida albicans.

Abstract:

:Microsomal chitinase from yeast and hyphal cells of Candida albicans was activated endogenously by incubation at 30 degrees C and exogenously by trypsin. The putative activating factor of yeast cells was separated from chitinase activity by fractionation of lysed protoplasts on an Iodixanol density gradient. The vacuole fraction contained no significant chitinase activity, but was enriched in chitinase activating factor. Activity of microsomal chitinase increased upon incubation with this, but no other gradient factor. Results suggest that the regulatory system governing microsomal chitinase activity, like that governing chitin synthase, involves a 'vacuolar' activating factor in Candida albicans.

journal_name

Lett Appl Microbiol

authors

Jackson DJ,Saunders VA,Humphreys AM

doi

10.1111/j.1472-765x.1996.tb00054.x

subject

Has Abstract

pub_date

1996-09-01 00:00:00

pages

159-62

issue

3

eissn

0266-8254

issn

1472-765X

journal_volume

23

pub_type

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