Pyrosequencing as a tool for the detection of Phytophthora species: error rate and risk of false Molecular Operational Taxonomic Units.

abstract：UNLABELLED:Several serological diagnostics rely on enzyme-linked immunosorbent assay (ELISA) to detect bacterial infections. However, for some pathogens, including Bartonella henselae, diagnosis still depends on manually intensive, time-consuming assays including micro-immunofluorescence, Western blotting or indirect i...

journal_title：Letters in applied microbiology

authors： Ferrara F,Di Niro R,D'Angelo S,Busetti M,Marzari R,Not T,Sblattero D

更新日期：2014-09-01 00:00:00

abstract：:Quillaja saponin (QS) was incubated at 39 degrees C in an in vitro medium containing rumen liquor from a cow fed a roughage diet. No degradation of QS was observed up to 6 h of fermentation. Incubation for 9, 12 and 24 h decreased the content of QS by 16%, 45% and 100%. The content of QS did not decrease when incubate...

journal_title：Letters in applied microbiology

authors： Makkar HP,Becker K

更新日期：1997-10-01 00:00:00

abstract：AIMS:To assess the ability of staphylococcal bacteriophage K to inhibit Staphylococcus aureus in raw milk. METHODS AND RESULTS:The ability of bacteriophage (phage) to replicate in milk is important in situations where phage might be used as a therapeutic for bovine mastitis. Phage K was able to replicate normally, lea...

journal_title：Letters in applied microbiology

authors： O'Flaherty S,Coffey A,Meaney WJ,Fitzgerald GF,Ross RP

更新日期：2005-01-01 00:00:00

abstract：:The aim of this study was to determine the epidemiology (location, microbial load, microbiome, presence/absence of biofilm and pathogens, including ESKAPE-Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter species, and antimicrobial susc...

journal_title：Letters in applied microbiology

authors： Costa DM,Johani K,Melo DS,Lopes LKO,Lopes Lima LKO,Tipple AFV,Hu H,Vickery K

更新日期：2019-04-01 00:00:00

abstract：:Colicin V is a small, proteinaceous bacterial toxin, produced by many strains of Escherichia coli and other members of the Enterobacteriaceae, that fits the definition of class II bacteriocins of Gram-positive bacteria. Export of colicin V is dependent on specific ABC (ATP-binding cassette) secretion proteins which re...

journal_title：Letters in applied microbiology

authors： McCormick JK,Klaenhammer TR,Stiles ME

更新日期：1999-07-01 00:00:00

abstract：:Microsomal chitinase from yeast and hyphal cells of Candida albicans was activated endogenously by incubation at 30 degrees C and exogenously by trypsin. The putative activating factor of yeast cells was separated from chitinase activity by fractionation of lysed protoplasts on an Iodixanol density gradient. The vacuo...

journal_title：Letters in applied microbiology

authors： Jackson DJ,Saunders VA,Humphreys AM

更新日期：1996-09-01 00:00:00

abstract：AIM:To determine if the outer membrane (OM) cytochromes OmcA and OmcB of the metal-reducing bacterium Shewanella oneidensis MR-1 have distinct or overlapping roles in the reduction of insoluble manganese(IV) oxide. METHODS AND RESULTS:The gene replacement mutant (OMCA1) which lacks OmcA was partially deficient in Mn(I...

journal_title：Letters in applied microbiology

authors： Myers JM,Myers CR

更新日期：2003-01-01 00:00:00

abstract：AIMS:To provide evidence that DNA-PCR diagnostics of oral pathogens based on standard sample collection by paper point insertion from the depth of the periodontal pocket can be replaced by a novel non-invasive collection method based on swab technique from the gingiva. METHODS AND RESULTS:In this study we compared the...

journal_title：Letters in applied microbiology

authors： Smola SF,Rettenberger G,Simmet T,Burysek L

更新日期：2003-01-01 00:00:00

abstract：:A series of experiments was conducted to identify the molecular species responsible for surface active emulsification (surfactant) bioactivity in Bacillus subtilis subsp. subtilis strain ATCC PTA-125135, and to describe culture conditions to support the enriched production of said bioactivity in cultured plaque of the...

journal_title：Letters in applied microbiology

authors： Krueger LA,Grzemski MA,Bilyeu MC,Horst JG,Ugrin SA,Hosette CA,Spangler DA,Ayangbile GA

更新日期：2020-11-01 00:00:00

abstract：:Reagent-derived contamination can compromise the integrity of microbiome data, particularly in low microbial biomass samples. This contamination has recently been attributed to the 'kitome' (contamination introduced by the DNA extraction kit), prior to which attention was mostly paid to potential contamination introdu...

journal_title：Letters in applied microbiology

authors： Stinson LF,Keelan JA,Payne MS

更新日期：2019-01-01 00:00:00

abstract：:In order to select a suitable indicator for monitoring the pathogen regrowth potential of composted biosolids, the growth kinetics of selected bacteria were investigated. Growth parameters of six serovars of Salmonella and three strains of Escherichia coli in sterilized compost were compared. Seeded Salmonella and E. ...

journal_title：Letters in applied microbiology

authors： Sidhu J,Gibbs RA,Ho GE,Unkovich I

更新日期：1999-11-01 00:00:00

abstract：:Recent studies have shown that Salmonella shedding status affects sows' microbiota during gestation and that these modifications are reflected in the faecal microbiota of their piglets at weaning. The aims of this study were: (a) to evaluate the persistence, up to the fattening period, of the previously measured link ...

journal_title：Letters in applied microbiology

authors： Larivière-Gauthier G,Thibodeau A,Yergeau É,Fravalo P

更新日期：2021-02-01 00:00:00

abstract：AIMS:This work was carried out to study the acid production by Lactococcus lactis subsp. lactis strains isolated from goat's milk and goat cheese (Valdeteja variety) in order to select a suitable starter culture for industrial goat cheese manufacturing. METHODS AND RESULTS:The titrable acidity of 45 Lactococcus lactis...

journal_title：Letters in applied microbiology

authors： Alonso-Calleja C,Carballo J,Capita R,Bernardo A,García-López ML

更新日期：2002-01-01 00:00:00

abstract：AIMS:To characterize and identify a novel contaminant of aviation fuel. METHODS AND RESULTS:Micro-organisms (yeasts and bacteria) were isolated from samples of aviation fuel. A yeast that proved to have been unrecorded previously was isolated from more than one fuel sample. This novel yeast proved to be a new species ...

journal_title：Letters in applied microbiology

authors： Buddie AG,Bridge PD,Kelley J,Ryan MJ

更新日期：2011-01-01 00:00:00

abstract：:Yellow wine lees (YWL) are the main co-products in yellow wine industry with unbalanced amino acid (AA) profiles. Solid-state fermentation was employed in this study to upgrade the YWL for ruminant animals. A 3 × 3 orthogonal design was conducted to optimize the fermentation condition for optimal crude protein (CP) yi...

journal_title：Letters in applied microbiology

authors： Yao KY,Zhang TZ,Wang HF,Liu JX

更新日期：2018-12-01 00:00:00

abstract：AIMS:To identify and characterize amino acid transport in Leuconostoc mesenteroides. METHODS AND RESULTS:The transport of labelled amino acids was measured in whole cells of Leuc. mesenteroides CNRZ 1273. Systems were operative under physiological conditions of growth, energy dependent and differed from peptide transp...

journal_title：Letters in applied microbiology

authors： Gendrot F,Foucaud-Scheunemann C,Ferchichi M,Hemme D

更新日期：2002-01-01 00:00:00

abstract：AIMS:To measure the microbial removal capacity of a small-scale hydroponics wastewater treatment plant. METHODS AND RESULTS:Paired samples were taken from untreated, partly-treated and treated wastewater and analysed for faecal microbial indicators, i.e. coliforms, Escherichia coli, enterococci, Clostridium perfringen...

journal_title：Letters in applied microbiology

authors： Ottoson J,Norström A,Dalhammar G

更新日期：2005-01-01 00:00:00

abstract：AIMS:The aims of this study are to obtain the draft genome sequence of Streptomyces coelicoflavus ZG0656, which produces novel acarviostatin family α-amylase inhibitors, and then to reveal the putative acarviostatin-related gene cluster and the biosynthetic pathway. METHODS AND RESULTS:The draft genome sequence of S. ...

journal_title：Letters in applied microbiology

authors： Guo X,Geng P,Bai F,Bai G,Sun T,Li X,Shi L,Zhong Q

更新日期：2012-08-01 00:00:00

abstract：:The present study aimed to investigate the incidence, virulence and antibiotic properties in Vibrio spp. isolated from cockles (Tegillarca granosa) marketed in Korea. A total of 32 Vibrio spp. isolates including V. parahaemolyticus (n = 4), V. alginolyticus (n = 11), V. diabolicus (n = 14) and V. harveyi (n = 3) were ...

journal_title：Letters in applied microbiology

authors： Dahanayake PS,Hossain S,Wickramanayake MVKS,Heo GJ

更新日期：2020-07-01 00:00:00

abstract：AIMS:The following polymers were developed: polyethylene (PE), a PE and polyethylene oxide (70% PE and 30% PEO; PE + PEO) blend, PE and nisin (PE + nisin), PE, nisin, and EDTA (PE + nisin + EDTA), and PE + PEO with nisin (PE + PEO + nisin). METHODS AND RESULTS:Of the polymers tested, PE and PE + PEO did not exhibit an...

journal_title：Letters in applied microbiology

authors： Cutter CN,Willett JL,Siragusa GR

更新日期：2001-10-01 00:00:00

abstract：AIMS:To develop and evaluate a real-time PCR assay incorporating an internal amplification control (IAC) suitable for the screening of Shiga toxin (Stx)-producing Escherichia coli (STEC) in foods. METHODS AND RESULTS:A competitive IAC was constructed and included in an stx-specific real-time PCR assay. Coupled to 18-h...

journal_title：Letters in applied microbiology

authors： Auvray F,Lecureuil C,Dilasser F,Taché J,Derzelle S

更新日期：2009-05-01 00:00:00

abstract：:The detection of foodborne viruses in bivalve molluscs is a challenging procedure in relation to low virus concentration and to the presence of significant RT-PCR inhibitors. The aim of this study was the development of an efficient direct extraction method for foodborne viral RNA from bivalve molluscs. Using Mengovir...

journal_title：Letters in applied microbiology

authors： Quang Le H,Suffredini E,Tien Pham D,Kim To A,De Medici D

更新日期：2018-11-01 00:00:00

abstract：:Campylobacter jejuni was inoculated into a range of raw and ready-to-eat foods. These food samples were used as a test source for detection of this bacterium by the polymerase chain reaction (PCR). Specific detection of Camp. jejuni, as indicated by a PCR product of 159 bp, was possible with all pre-cooked deli-sliced...

journal_title：Letters in applied microbiology

authors： Winters DK,O'Leary AE,Slavik MF

更新日期：1998-09-01 00:00:00

abstract：UNLABELLED:Cysteine phytase is the main phytate-degrading enzyme of ruminant animals. To explore the genetic diversity and dynamic expression profile of cysteine phytase in sheep rumen during a feeding cycle, four transcript (0, 4, 9 and 16 h after feeding) and one DNA (9 h after feeding) clone libraries were construct...

journal_title：Letters in applied microbiology

authors： Li Z,Huang H,Zhao H,Meng K,Zhao J,Shi P,Yang P,Luo H,Wang Y,Yao B

更新日期：2014-12-01 00:00:00

abstract：AIMS:This study aimed to identify a simple, inexpensive preservation technique that will allow a quick and reliable recovery of Campylobacter jejuni following long-term periods of preservation. METHODS AND RESULTS:Preservation techniques include (i) Cryobank microbial preservation system using hypertonic 'cryopreserva...

journal_title：Letters in applied microbiology

authors： Gorman R,Adley CC

更新日期：2004-01-01 00:00:00

abstract：:Irreversible electroporation (IRE) damages cell membranes and is used in medicine for nonthermal ablation of malignant tumours. Our aim was to evaluate the antimicrobial effect of IRE. The pathogenic micro-organisms, Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa and Candida al...

journal_title：Letters in applied microbiology

authors： Korem M,Goldberg NS,Cahan A,Cohen MJ,Nissenbaum I,Moses AE

更新日期：2018-07-01 00:00:00

abstract：AIMS:The aim of this study was to determine the prevalence and proportions of opportunistic pathogens harboured on orthodontic retainers. METHODS AND RESULTS:First, Staphylococcus spp. and Candida spp. were isolated from the retainer's inner surface and from other mucosal surfaces of the subject's mouth by routine bac...

journal_title：Letters in applied microbiology

authors： Al Groosh D,Roudsari GB,Moles DR,Ready D,Noar JH,Pratten J

更新日期：2011-05-01 00:00:00

abstract：AIMS:To test the suitability of cheese whey powder (CWP) solution for ethanol fermentation and to compare performances of different Kluyveromyces marxianus strains for ethanol fermentation from CWP solution. METHODS AND RESULTS:Batch ethanol fermentation of cheese whey (CW), CWP and lactose solutions with the same ini...

journal_title：Letters in applied microbiology

authors： Ozmihci S,Kargi F

更新日期：2007-06-01 00:00:00

abstract：:Ergosterol biosynthesis in Saccharomyces cerevisiae is complex and the underlying mechanism of regulation remains unclear. To clarify the influence of transcriptional regulation on the ergosterol content, transcription factor Ecm22 was overexpressed in S. cerevisiae. Results showed that the overexpression of ECM22 led...

journal_title：Letters in applied microbiology

authors： Wang SQ,Wang T,Liu JF,Deng L,Wang F

更新日期：2018-11-01 00:00:00

abstract：:The cell surface hydrophobicity of micro-organisms is a characteristic that has been associated with the colonization of mammalian epithelia and with their capacity to induce diseases. Melatonin is a hormone produced by the pineal gland that affects the immune response mechanism. This study investigated, as an express...

journal_title：Letters in applied microbiology

authors： Uberos J,Molina A,Liébana J,Augustin MC,Muñoz A

更新日期：2000-10-01 00:00:00