Development of a method for direct extraction of viral RNA from bivalve molluscs.

abstract：:Candida-associated denture stomatitis presents as erythema of the palatal mucosa and is caused by biofilms containing the fungus Candida albicans that co-reside with oral bacteria on the denture-fitting surface. This study aimed to assess the effect of several frequently encountered oral bacteria on the expression of ...

journal_title：Letters in applied microbiology

authors： Morse DJ,Wilson MJ,Wei X,Bradshaw DJ,Lewis MAO,Williams DW

更新日期：2019-04-01 00:00:00

abstract：:Suitable conditions for the introduction of bacteriophage DNA into cells of Rhodococcus rhodochrous CF222 by electroporation were established, and penicillin G was found to enhance the transfection frequency. When conditions optimal for the parental strain were applied to its colony-morphological mutants, different tr...

journal_title：Letters in applied microbiology

authors： Sunairi M,Iwabuchi N,Murakami K,Watanabe F,Ogawa Y,Murooka H,Nakajima M

更新日期：1996-01-01 00:00:00

abstract：:The effect of liquid mechanical vibration on the adhesion of Saccharomyces cerevisiae cells to the internal glass surface of a pipette was studied using a 25 Hz vibration source. The maximum vibration amplitude was 1.06 mm (peak to peak) along the pipette direction. Relative movements between the pipette and yeast sus...

journal_title：Letters in applied microbiology

authors： Jiang C,Yu H,Ding Z,Ma Y

更新日期：1996-07-01 00:00:00

abstract：:Autoaggregation was frequently encountered among intestinal lactobacilli isolated from weaned pigs. The aggregation mechanism was shown to be mediated by the production of a proteinaceous aggregation-promoting factor in two strains of Lactobacillus reuteri. A 32 kDa aggregation-promoting protein was detected in these ...

journal_title：Letters in applied microbiology

authors： Kmet V,Callegari ML,Bottazzi V,Morelli L

更新日期：1995-12-01 00:00:00

abstract：AIMS:To find a supplemental ingredient that can be added to routinely used growth media to increase conidial production and decrease aflatoxin biosynthesis in small sclerotial (S strain) isolates of Aspergillus flavus. METHODS AND RESULTS:Molasses was added to three commonly used culture media: coconut agar (CAM), pot...

journal_title：Letters in applied microbiology

authors： Chang PK,Hua SS

更新日期：2007-02-01 00:00:00

abstract：AIMS:To investigate a lactococcal plasmid responsible for a reduction in growth rate of its host cell. METHODS AND RESULTS:Lactococcus lactis subsp. lactis biovar. diacetylactis DRC1 carries a high number of plasmids. The DRC1 wild-type strain was found to grow more slowly than a plasmid-free derivative of DRC1. The p...

journal_title：Letters in applied microbiology

authors： Kobayashi M,Nomura M,Fujita Y,Okamoto T,Ohmomo S

更新日期：2002-01-01 00:00:00

abstract：AIMS:The present study was aimed to develop a loop-mediated isothermal amplification (LAMP) assay for rapid and specific detection of Vibrio cholerae. METHODS AND RESULTS:A set of five designed primers that recognized specifically the V. cholerae ompW gene was used. The optimized time and temperature conditions for th...

journal_title：Letters in applied microbiology

authors： Srisuk C,Chaivisuthangkura P,Rukpratanporn S,Longyant S,Sridulyakul P,Sithigorngul P

更新日期：2010-01-01 00:00:00

abstract：:High performance thin layer chromatography with automated multiple development was used to determine 2,3-butanediol levels in wine produced by high and low acetoin-forming strains of Saccharomyces cerevisiae. An inverse correlation between acetoin and 2,3-butanediol content was found suggesting a leaky mutation in ace...

journal_title：Letters in applied microbiology

authors： Romano P,Suzzi G,Brandolini V,Menziani E,Domizio P

更新日期：1996-04-01 00:00:00

abstract：AIMS:Clostridium perfringens is recommended as a suitable indicator bacterium for human enteric viruses, Giardia cysts and Cryptosporidium oocysts in finished water and in the assessment and evaluation of water treatment. Several agars and confirmation procedures were evaluated in parallel with the Australian/New Zeala...

journal_title：Letters in applied microbiology

authors： Wohlsen T,Bayliss J,Gray B,Bates J,Katouli M

更新日期：2006-05-01 00:00:00

abstract：AIMS:To isolate gamma-hexachlorocyclohexane (gamma-HCH)-degrading bacteria from a single field and to examine their genetic diversity. METHODS AND RESULTS:Gamma-HCH-degrading bacteria were screened from a long-term experimental field in which gamma-HCH has been continuously applied to, and a gamma-HCH-degrading sphing...

journal_title：Letters in applied microbiology

authors： Yamamoto S,Otsuka S,Murakami Y,Nishiyama M,Senoo K

更新日期：2009-10-01 00:00:00

abstract：UNLABELLED:Human bikunin is a glycoprotein that exhibits trypsin inhibitory activity against serine proteases, and is effective in clinic. However, limited productivity and high price of human bikunin retard its further application. In this study, a high-yield, low-cost process of recombinant human bikunin (rh-bikunin)...

journal_title：Letters in applied microbiology

authors： Yao M,Zhang J,Wang X

更新日期：2015-10-01 00:00:00

abstract：:Adhesion of three Lactobacillus strains onto human epithelial intestinal Caco-2 and Int-407 cell lines was compared. More adhesion occurred onto Int-407. The trypsin and sodium periodate pretreatment of bacteria revealed different mechanisms of adhesion depending on the Caco-2 and Int-407, involving carbohydrates and ...

journal_title：Letters in applied microbiology

authors： Sarem F,Sarem-Damerdji LO,Nicolas JP

更新日期：1996-06-01 00:00:00

abstract：:A rapid and simple method (24M) using 24 well microtitre plates was developed to determine the presence of Listeria monocytogenes or Listeria spp. in food samples. The 24M was composed of two 24 well microtitre plates connected with a yellow tip. The 24M was evaluated with pathogen cocktails and ground beef samples an...

journal_title：Letters in applied microbiology

authors： Kang DH,Fung DY

更新日期：1999-04-01 00:00:00

abstract：AIMS:Immunologically based assays for the detection of staphylococcal enterotoxins are numerous. These techniques include radio immunosorbent assays and enzyme-linked immunosorbent assays (ELISA), some of which are available as commercial kits. The purpose of this study was to compare the performances of three commerci...

journal_title：Letters in applied microbiology

authors： Vernozy-Rozand C,Mazuy-Cruchaudet C,Bavai C,Richard Y

更新日期：2004-01-01 00:00:00

abstract：:Enterococci were isolated from faecal droppings of chickens in broiler and layer farms and the susceptibilities to nine therapeutic antimicrobial agents and six growth-promoting antibiotics were determined by the agar dilution method. Resistance to therapeutic antimicrobial agents such as ampicillin, clindamycin, eryt...

journal_title：Letters in applied microbiology

authors： Yoshimura H,Ishimaru M,Endoh YS,Kojima A

更新日期：2000-12-01 00:00:00

abstract：:PCR primers were selected from the flagellin gene sequences flaA and flaB of Campylobacter coli to amplify DNA from Camp. jejuni and Camp. coli. When the PCR products were analysed by hybridization to an internal probe immobilized in microtitre wells, positive reactions were observed only for strains of Camp. jejuni a...

journal_title：Letters in applied microbiology

authors： Rasmussen HN,Olsen JE,Jørgensen K,Rasmussen OF

更新日期：1996-11-01 00:00:00

abstract：UNLABELLED:Fast microbial identification is becoming increasingly necessary in industry to improve microbial control and reduce biocide consumption. We compared the performances of two systems based on MALDI-TOF MS (VITEK MS and BIOTYPER) and two based on biochemical testing (BIOLOG, VITEK 2 Compact) with genetic metho...

journal_title：Letters in applied microbiology

authors： Urwyler SK,Glaubitz J

更新日期：2016-02-01 00:00:00

abstract：:Nisin, a bacteriocin produced by some strains of Lactococcus lactis, acts against foodborne pathogen Listeria monocytogenes. A single exposure of cells to nisin can generate nisin-resistant (Nisr) mutants, which may compromise the use of nisin in the food industry. The objective of this research was to compare the hea...

journal_title：Letters in applied microbiology

authors： Modi KD,Chikindas ML,Montville TJ

更新日期：2000-03-01 00:00:00

abstract：AIMS:  To show that application of copper hydroxide citrus sprays mixed with field source water (possibly contaminated) will not support Escherichia coli on plant surfaces. Environmental stresses of transient phyllosphere bacteria and presence of copper will eradicate these bacteria before harvest. METHODS AND RESULTS...

journal_title：Letters in applied microbiology

authors： Narciso JA,Ference CM,Ritenour MA,Widmer WW

更新日期：2012-02-01 00:00:00

abstract：UNLABELLED:Cryptococcosis, a fungal infection that affects both immunocompromised and immunocompetent individuals, contributes to increasing indices of mortality and morbidity. The development of resistance by Cryptococcus spp., the limited number of commercial antifungal drugs and the various side effects of these dru...

journal_title：Letters in applied microbiology

authors： Magalhães TF,da Silva CM,de Fátima Â,da Silva DL,Modolo LV,Martins CV,Alves RB,Ruiz AL,Longato GB,de Carvalho JE,de Resende-Stoianoff MA

更新日期：2013-08-01 00:00:00

abstract：AIMS:Aim of the study was to develop a medium for optimal heparinase production with a strain of Aspergillus flavus (MTCC-8654) by using a multidimensional statistical approach. METHODS AND RESULTS:Statistical optimization of intracellular heparinase production by A. flavus, a new isolate, was investigated. Plackett-B...

journal_title：Letters in applied microbiology

authors： Banga J,Tripathi CK

更新日期：2009-08-01 00:00:00

abstract：:RAPD technique (randomly amplified polymorphic DNA) was used for epidemiological subtyping of Bacillus licheniformis and other Bacillus spp. Within 46 isolates of B. licheniformis, up to 10 strain types could be determined when two 10-mer primers were used. RAPD patterns, which were found in eight further strains of B...

journal_title：Letters in applied microbiology

authors： Stephan R,Schraft H,Untermann F

更新日期：1994-05-01 00:00:00

abstract：AIMS:To determine the production of reuterin by lactobacilli isolates from pig faeces and to evaluate their potential as probiotic bacteria. METHODS AND RESULTS:Twenty-eight of 165 lactobacilli isolates produced reuterin in the presence of glycerol. Six isolates yielding high levels of reuterin with respect to type st...

journal_title：Letters in applied microbiology

authors： Rodríguez E,Arqués JL,Rodríguez R,Nuñez M,Medina M

更新日期：2003-01-01 00:00:00

abstract：AIMS:To test the efficacy of Lactobacillus johnsonii FI9785 in reducing the colonization and shedding of Salmonella enterica serotype Enteritidis, Escherichia coli O78:K80 and Clostridium perfringens in poultry. METHODS AND RESULTS:Specific pathogen-free chicks (1 day old) were dosed with a single oral inoculum of 1x1...

journal_title：Letters in applied microbiology

authors： La Ragione RM,Narbad A,Gasson MJ,Woodward MJ

更新日期：2004-01-01 00:00:00

abstract：AIM:The mechanisms of adaptation of Clostridium perfringens to high temperatures are not well understood. In this work, the involvement of extracellular compounds in protection to heat was determined. METHODS AND RESULTS:Cells were grown in fluid thioglycollate medium or chicken broth. When mid-log phase was reached, ...

journal_title：Letters in applied microbiology

authors： Heredia N,Ybarra P,Hernández C,García S

更新日期：2009-01-01 00:00:00

abstract：AIMS:The study evaluated the efficacy of four Mycobacterium avium subsp. paratuberculosis (MAP) culture media in suppressing commonly used starter cultures and typical nonstarter microflora present during the manufacture and ripening of Cheddar cheese, with a view to identify a suitable medium for the enumeration of MA...

journal_title：Letters in applied microbiology

authors： Donaghy JA,Totton NL,Rowe MT

更新日期：2003-01-01 00:00:00

abstract：AIMS:  This paper utilized quantitative LC-MS/MS to profile the short-chain acyl-CoA levels of several strains of Escherichia coli engineered for heterologous polyketide production. To further compare and potentially expand the levels of available acyl-CoA molecules, a propionyl-CoA synthetase gene from Ralstonia solan...

journal_title：Letters in applied microbiology

authors： Armando JW,Boghigian BA,Pfeifer BA

更新日期：2012-02-01 00:00:00

abstract：AIMS:Transmission of microbial pathogens to patients from water in dental units is a concern. To reduce this risk, the decontaminating efficiency of hydrogen peroxide was evaluated. METHODS AND RESULTS:Three percent hydrogen peroxide diluted 1 : 4 in distilled water (contact time 15 min) was used daily to disinfect th...

journal_title：Letters in applied microbiology

authors： Zanetti F,De Luca G,Tarlazzi P,Stampi S

更新日期：2003-01-01 00:00:00

abstract：AIMS:Oenococcus oeni is a lactic acid bacterium used in wine fermentation. Two open reading frames (orfB and orfC) were identified in the upstream region of the hsp18 gene, encoding the small heat-shock protein Lo18. Expression of these genes in conditions of acid stress was studied in Escherichia coli. METHODS AND RE...

journal_title：Letters in applied microbiology

authors： Morel F,Delmas F,Jobin MP,Diviès C,Guzzo J

更新日期：2001-08-01 00:00:00

abstract：AIMS:The aim of the study was to determine Campylobacter jejuni contamination and prevalence on fomites moving between broiler farms and the processing plant in the period after cleaning and before departure to harvest chickens. In addition, changes in the proportion of contaminated fomites in the course of a day were ...

journal_title：Letters in applied microbiology

authors： Ramabu SS,Boxall NS,Madie P,Fenwick SG

更新日期：2004-01-01 00:00:00