Extracellular protectants produced by Clostridium perfringens cells at elevated temperatures.

abstract：AIMS:The purpose of this study was to determine if DNA polymorphisms generated by RAPD-PCR could be used to characterize Group B streptococci (GBS) for epidemiological purposes. METHODS AND RESULTS:30 unrelated, previously serotyped strains were analysed by RAPD-PCR using two 10-mer primers (5' TGCGAGAGTC 3' and 5' AG...

journal_title：Letters in applied microbiology

authors： Zhang GW,Kotiw M,Daggard G

更新日期：2002-01-01 00:00:00

abstract：AIMS:To develop a sensitive, rapid and simple method for detection of Botrytis cinerea based on loop-mediated isothermal amplification (LAMP) that would be suitable for use outside a conventional laboratory setting. METHODS AND RESULTS:A LAMP assay was designed based on the intergenic spacer of the B. cinerea nuclear ...

journal_title：Letters in applied microbiology

authors： Tomlinson JA,Dickinson MJ,Boonham N

更新日期：2010-12-01 00:00:00

abstract：AIMS: To develop a new instrument of great versatility for recovering micro-organisms from all types of food samples and to compare the effects with existing sample preparation methods. METHODS AND RESULTS: To detach micro-organisms from large-size unbroken food samples such as apples, carrots, potatoes and tomatoes w...

journal_title：Letters in applied microbiology

authors： Kim YH,Lee SY,Sagong HG,Heu S,Ryu S,Kang DH

更新日期：2012-09-01 00:00:00

abstract：AIMS:The aim of this study was to identify and determine the diversity, occurrence and distribution of fungi in water used at a haemodialysis centre. METHODS AND RESULTS:Samples in the hydraulic circuit for the distribution of the water, dialysate samples and samples of sterilization solution from dialysers were colle...

journal_title：Letters in applied microbiology

authors： Pires-Gonçalves RH,Sartori FG,Montanari LB,Zaia JE,Melhem MS,Mendes-Giannini MJ,Martins CH

更新日期：2008-05-01 00:00:00

abstract：:Reagent-derived contamination can compromise the integrity of microbiome data, particularly in low microbial biomass samples. This contamination has recently been attributed to the 'kitome' (contamination introduced by the DNA extraction kit), prior to which attention was mostly paid to potential contamination introdu...

journal_title：Letters in applied microbiology

authors： Stinson LF,Keelan JA,Payne MS

更新日期：2019-01-01 00:00:00

abstract：:The fate of Salmonella enteritidis PT4 in home-made mayonnaise prepared with citric acid solution (citric acid concentration of > or = 4.98% (w/v)) was investigated. It was found that pH of mayonnaise is closely related to the ratio of egg yolk to citric acid, and the inactivation rate of the micro-organisms increases...

journal_title：Letters in applied microbiology

authors： Xiong R,Xie G,Edmondson AS

更新日期：1999-01-01 00:00:00

abstract：AIMS:The gram-positive bacterial genus Lactococcus has been taxonomically classified into seven species (Lactococcus lactis, Lactococcus garvieae, Lactococcus piscium, Lactococcus plantarum, Lactococcus raffinolactis, Lactococcus chungangensis and Lactococcus fujiensis). This study aimed to develop a novel multiplex po...

journal_title：Letters in applied microbiology

authors： Odamaki T,Yonezawa S,Kitahara M,Sugahara Y,Xiao JZ,Yaeshima T,Iwatsuki K,Ohkuma M

更新日期：2011-05-01 00:00:00

abstract：:The objective of this study was to undertake a microbiological survey of foods, animal faeces and wastewater samples for Clostridium difficile, and determine the genotypes and antimicrobial susceptibilities of isolates. A total of 211 samples were tested for C. difficile using culture methods. Thirteen toxigenic C. di...

journal_title：Letters in applied microbiology

authors： Rivas L,Dupont PY,Gilpin BJ,Cornelius AJ

更新日期：2020-01-01 00:00:00

abstract：:The novel Eriocheir sinensis reovirus (EsRV) is a pathogen that causes severe disease and high mortality rates in cultivated crabs. Here, we established a highly sensitive and specific rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that was cheaper and more suitable for field applic...

journal_title：Letters in applied microbiology

authors： Ma Y,Dai T,Serwadda A,Shen H

更新日期：2016-11-01 00:00:00

abstract：AIMS:To determine the production of reuterin by lactobacilli isolates from pig faeces and to evaluate their potential as probiotic bacteria. METHODS AND RESULTS:Twenty-eight of 165 lactobacilli isolates produced reuterin in the presence of glycerol. Six isolates yielding high levels of reuterin with respect to type st...

journal_title：Letters in applied microbiology

authors： Rodríguez E,Arqués JL,Rodríguez R,Nuñez M,Medina M

更新日期：2003-01-01 00:00:00

abstract：AIMS:To isolate benzene-degrading strains from neutral and alkaline groundwaters contaminated by benzene, toluene, ethylbenzene, xylenes (BTEX) from the SIReN aquifer, UK, and to test their effective pH range and ability to degrade TEX. METHODS AND RESULTS:The 14 isolates studied had an optimum pH for growth of 8, and...

journal_title：Letters in applied microbiology

authors： Fahy A,Ball AS,Lethbridge G,Timmis KN,McGenity TJ

更新日期：2008-07-01 00:00:00

abstract：:Flow cytometry in combination with fluorescent molecular markers 5- (and 6-) carboxyfluorescein succinimidylester (CFSE) and propidium iodide (PI) have been applied to determine lag times, numbers of cell divisions and injury after mild heat (50 degrees C, 5 min) and nisin treatments (0.1 and 1.0 microgram ml-1) of La...

journal_title：Letters in applied microbiology

authors： Ueckert JE,Nebe von-Caron G,Bos AP,ter Steeg PF

更新日期：1997-10-01 00:00:00

abstract：UNLABELLED:Most plant species are colonized by endophytic bacteria. Despite their importance for plant health and growth, the response of these bacteria to grassland management regimes is still not understood. Hence, we investigated the bacterial community structure in three agricultural important grass species Dactyli...

journal_title：Letters in applied microbiology

authors： Wemheuer F,Wemheuer B,Kretzschmar D,Pfeiffer B,Herzog S,Daniel R,Vidal S

更新日期：2016-04-01 00:00:00

abstract：AIMS:The genetic diversity of Vibrio cholerae isolated from black tiger shrimp (Penaeus monodon) aquaculture farms was determined using three PCR typing methods based on enterobacterial repetitive intergenic consensus (ERIC) sequences, ribosomal gene spacer (RS) sequence and repetitive extragenic palindromic (REP) sequ...

journal_title：Letters in applied microbiology

authors： Rao BM,Surendran PK

更新日期：2010-07-01 00:00:00

abstract：:Listeria monocytogenes is a pathogenic bacterium, with human disease and infection linked to dairy products, seafood, ready-to-eat meat and raw & undercooked meats. Stainless steel is the most common food preparation surface and therefore, it is important to understand how food storage conditions such as surface mater...

journal_title：Letters in applied microbiology

authors： Redfern J,Verran J

更新日期：2017-04-01 00:00:00

abstract：:In this study, a total of 172 putative omega-3 producers were isolated from 28 sediment samples from the Arabian Gulf employing a selective isolation procedure using marine agar containing 0·1% triphenyl tetrazolium chloride (TTC). Out of these 172 isolates, 19 isolates produced eicosapentaenoic acid (EPA) as confirme...

journal_title：Letters in applied microbiology

authors： Alagarsamy S,Sabeena Farvin KH,Fakhraldeen S,Kooramattom MR,Al-Yamani F

更新日期：2019-08-01 00:00:00

abstract：AIMS:To study the cellular growth and morphology of Yarrowia lipolytica W29 and its lipase and protease production under increased air pressures. METHODS AND RESULTS:Batch cultures of the yeast were conducted in a pressurized bioreactor at 4 and 8 bar of air pressure and the cellular behaviour was compared with cultur...

journal_title：Letters in applied microbiology

authors： Lopes M,Gomes N,Gonçalves C,Coelho MA,Mota M,Belo I

更新日期：2008-02-01 00:00:00

abstract：AIMS:Real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on a TaqMan-minor groove binder (MGB) probe was developed for the rapid detection of avian influenza virus subtype H5. METHODS AND RESULTS:Conserved regions in the haemagglutinin genes of avian influenza viruses subtype H5 served as ta...

journal_title：Letters in applied microbiology

authors： Lu YY,Yan JY,Feng Y,Xu CP,Shi W,Mao HY

更新日期：2008-01-01 00:00:00

abstract：AIMS:Aeromonas hydrophila is recognized as a human pathogen following wound exposure or ingestion of contaminated water and food. For rapid identification of this bacterium, a TaqMan-based real-time PCR assay has been developed. METHODS AND RESULTS:Primers and probes that target specific sequences of the 16S rRNA gene...

journal_title：Letters in applied microbiology

authors： Trakhna F,Harf-Monteil C,Abdelnour A,Maaroufi A,Gadonna-Widehem P

更新日期：2009-08-01 00:00:00

abstract：:Colistin-resistant Pseudomonas aeruginosa (CRPA), as a health care system threat, is increasing globally. This study aimed was to determine CRPA by high-resolution melting curve (HRM) analysis method. The HRM method was done on standard strains of P. aeruginosa and CRPA strains. Ninefold serial dilutions of known geno...

journal_title：Letters in applied microbiology

authors： Tahmasebi H,Dehbashi S,Arabestani MR

更新日期：2020-04-01 00:00:00

abstract：:Leuconostoc strains were characterized according to their antibiotic susceptibilities, carbohydrate fermentation profiles, sucrase activity patterns and plasmid content. All the strains tested were resistant to the antibiotics sulphathiazole, trimethoprim and vancomycin, and could be separated into two groups based on...

journal_title：Letters in applied microbiology

authors： Holt SM,Al-Sheikh H,Shin KJ

更新日期：2001-03-01 00:00:00

abstract：AIMS: To develop a specific and highly sensitive loop-mediated isothermal amplification (LAMP) technique for the rapid detection of canine parvovirus (CPV) DNA directly in suspected faecal samples of dogs by employing a simple method of template preparation. METHODS AND RESULTS: LAMP reaction was developed by designin...

journal_title：Letters in applied microbiology

authors： Mukhopadhyay HK,Amsaveni S,Matta SL,Antony PX,Thanislass J,Pillai RM

更新日期：2012-09-01 00:00:00

abstract：AIMS:To isolate and identify diazotrophic endophytes in the stem of Japanese sweetpotato cv. Koganesengan. METHODS AND RESULTS:Surface-sterilized and thinly sliced (1-2 mm) sweetpotato stem samples were incubated in test tubes with semi-solid modified Rennie (MR) medium. The test tubes were assayed for acetylene reduc...

journal_title：Letters in applied microbiology

authors： Asis CA Jr,Adachi K

更新日期：2004-01-01 00:00:00

abstract：:Ergosterol biosynthesis in Saccharomyces cerevisiae is complex and the underlying mechanism of regulation remains unclear. To clarify the influence of transcriptional regulation on the ergosterol content, transcription factor Ecm22 was overexpressed in S. cerevisiae. Results showed that the overexpression of ECM22 led...

journal_title：Letters in applied microbiology

authors： Wang SQ,Wang T,Liu JF,Deng L,Wang F

更新日期：2018-11-01 00:00:00

abstract：:Thirty-eight isolates of Hafnia alvei were characterized by biochemical profiles, ribotyping and pulsed-field gel electrophoresis (PFGE) patterns. The isolates were recovered from chub-packed (19 isolates) or retail (nine isolates) ground beef, or were obtained from culture repositories (10 isolates). Biochemical prof...

journal_title：Letters in applied microbiology

authors： Gamage SD,Luchansky JB,Ingham SC

更新日期：1998-02-01 00:00:00

abstract：AIMS:The use of randomly amplified polymorphic DNA (RAPD)-PCR fingerprinting and plasmid profiles to determine at the strain level, the similarity of Lactococcus lactis isolates obtained during sampling of traditional cheeses and to verify its correspondence to the selected phenotypic characteristics. METHODS AND RESU...

journal_title：Letters in applied microbiology

authors： de la Plaza M,Rodríguez A,Fernández de Palencia P,Martínez-Cuesta MC,Peláez C,Requena T

更新日期：2006-12-01 00:00:00

abstract：:Alzheimer's disease (AD) is the most common form of dementia and one of the major causes of disability and dependency in older people. Accumulating evidences link gut microbiota with different diseases and its relationship with neurodegenerative diseases is becoming most intriguing. This study was aimed to compare the...

journal_title：Letters in applied microbiology

authors： Bäuerl C,Collado MC,Diaz Cuevas A,Viña J,Pérez Martínez G

更新日期：2018-06-01 00:00:00

abstract：UNLABELLED:The mechanisms and organisms involved in the natural formation of volatile organohalogen compounds (VOX) are largely unknown. We provide evidence that the common and widespread soil bacterium Sinorhizobium meliloti strain 1021 is capable of producing up to 3338·6 ± 327·8 ng l(-1) headspace volume of chlorofo...

journal_title：Letters in applied microbiology

authors： Weigold P,Ruecker A,Jochmann M,Osorio Barajas XL,Lege S,Zwiener C,Kappler A,Behrens S

更新日期：2015-10-01 00:00:00

abstract：AIMS:To measure the microbial removal capacity of a small-scale hydroponics wastewater treatment plant. METHODS AND RESULTS:Paired samples were taken from untreated, partly-treated and treated wastewater and analysed for faecal microbial indicators, i.e. coliforms, Escherichia coli, enterococci, Clostridium perfringen...

journal_title：Letters in applied microbiology

authors： Ottoson J,Norström A,Dalhammar G

更新日期：2005-01-01 00:00:00

abstract：:The neurotoxins produced by Clostridium botulinum are amongst the most potent known to man. Toxin production is detected by a mouse bioassay, which requires several days for a result and is not acceptable for routine use unless there is a high level of suspicion. The Rapid ID32 A kit produced by bioMerieux gives an id...

journal_title：Letters in applied microbiology

authors： Brett MM

更新日期：1998-01-01 00:00:00