Modulation of Candida albicans virulence in in vitro biofilms by oral bacteria.

Abstract:

:Candida-associated denture stomatitis presents as erythema of the palatal mucosa and is caused by biofilms containing the fungus Candida albicans that co-reside with oral bacteria on the denture-fitting surface. This study aimed to assess the effect of several frequently encountered oral bacteria on the expression of C. albicans virulence factors in in vitro polymicrobial biofilms. Biofilms containing C. albicans and selected bacterial species were grown on denture acrylic, and analysed by microscopy and by qPCR for expression of putative virulence genes. Candida albicans-only biofilms showed limited hyphal production. Hyphal development was significantly (P < 0·001) increased when biofilms also contained four species of oral bacteria (Streptococcus sanguinis, Streptococcus gordonii, Actinomyces odontolyticus and Actinomyces viscosus), as was the expression of virulence genes (P < 0·05). Importantly, inclusion of Porphyromonas gingivalis in the biofilm consortium resulted in significant (P < 0·05) inhibition of virulence gene expression and production of hyphae. The in vitro expression of C. albicans virulence factors was modulated in polymicrobial biofilms. The complexity of this modulation was highlighted by the reversal of effects following introduction of a single bacterial species into a biofilm community. SIGNIFICANCE AND IMPACT OF THE STUDY: The impact of individual bacterial species on Candida albicans virulence highlights both the complexity of predicting infection mediated by polymicrobial communities and the potential for management through pro- or prebiotic therapy. The possibility to selectively modulate microbial virulence by addition of, or treatment with pro- or prebiotics avoids the use of conventional antimicrobial compounds, thus reducing the contribution to potential drug resistance. Understanding which bacterial species modulate virulence, and the mechanisms by which this occurs, particularly in biofilms, provides excellent foundations for further research questions, and the potential for novel clinical interventions.

journal_name

Lett Appl Microbiol

authors

Morse DJ,Wilson MJ,Wei X,Bradshaw DJ,Lewis MAO,Williams DW

doi

10.1111/lam.13145

subject

Has Abstract

pub_date

2019-04-01 00:00:00

pages

337-343

issue

4

eissn

0266-8254

issn

1472-765X

journal_volume

68

pub_type

杂志文章
  • Establishing a campylobacter-free pig population through a top-down approach.

    abstract::Fattening pigs are often infected with campylobacter. To eliminate campylobacter from the pig population, a top-down approach, involving the breeding and reproduction farms, seems appropriate. In order to investigate the effectiveness of a top-down approach, sows' faeces from the following farms were analysed for the ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2000.00750.x

    authors: Weijtens MJ,Urlings HA,Van der Plas J

    更新日期:2000-06-01 00:00:00

  • Detection of coliphages and enteroviruses in sewage and aerosol from an activated sludge wastewater treatment plant.

    abstract::Coliphages and enteroviruses were monitored over 12 months in sewage and air adjacent to an activated sludge plant. Both showed temporal variation but the mean count of phages in enterovirus-positive samples was not significantly different from that in enterovirus-negative samples. Hence coliphages are not necessarily...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1995.tb01042.x

    authors: Carducci A,Arrighi S,Ruschi A

    更新日期:1995-09-01 00:00:00

  • Two-dimensional environmental profiles of growth, deoxynivalenol and nivalenol production by Fusarium culmorum on a wheat-based substrate.

    abstract:AIMS:To determine the effect of interacting conditions of water activity (aw, 0.99-0.85), temperature (15, 25 degrees C) and time (40 days) on growth and production of the mycotoxins deoxynivalenol (DON) and nivalenol (NIV) by Fusarium culmorum on a wheat-based agar medium. METHODS AND RESULTS:Fusarium culmorum grew o...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01358.x

    authors: Hope R,Magan N

    更新日期:2003-01-01 00:00:00

  • High-level secretion of human bikunin from recombinant Pichia pastoris.

    abstract:UNLABELLED:Human bikunin is a glycoprotein that exhibits trypsin inhibitory activity against serine proteases, and is effective in clinic. However, limited productivity and high price of human bikunin retard its further application. In this study, a high-yield, low-cost process of recombinant human bikunin (rh-bikunin)...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12470

    authors: Yao M,Zhang J,Wang X

    更新日期:2015-10-01 00:00:00

  • Antifungal activity of the amyrin derivatives and in vitro inhibition of Candida albicans adhesion to human epithelial cells.

    abstract:AIMS:The antifungal activity of amyrin pentacyclic triterpene and 15 synthetic derivatives was evaluated against Candida species. Additionally, inhibition of adhesion of Candida albicans to human epithelial cells in vitro was determined. METHODS AND RESULTS:Esterification of alpha- and beta-amyrin with a variety of ac...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02162.x

    authors: Johann S,Soldi C,Lyon JP,Pizzolatti MG,Resende MA

    更新日期:2007-08-01 00:00:00

  • Growth kinetics of aerobic granules developed in sequencing batch reactors.

    abstract:AIMS:This paper attempts to develop a kinetic model to describe the growth of aerobic granules developed under different operation conditions. METHODS AND RESULTS:A series of experiments were conducted by using four-column sequencing batch reactors to study the formation of aerobic granules under different conditions,...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.2003.01452.x

    authors: Yang SF,Liu QS,Tay JH,Liu Y

    更新日期:2004-01-01 00:00:00

  • The M. tuberculosis antigen 85 complex and mycolyltransferase activity.

    abstract:AIMS:The antigen 85 complex (Ag85) from Mycobacterium tuberculosis consists of three abundantly secreted proteins (FbpA, FbpB and FbpC2) which play a key role in the pathogenesis of tuberculosis and also exhibit cell wall mycolyltransferase activity. A related protein with similarity to the Ag85 complex was recently an...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01091.x

    authors: Kremer L,Maughan WN,Wilson RA,Dover LG,Besra GS

    更新日期:2002-01-01 00:00:00

  • Removal of micro-organisms in a small-scale hydroponics wastewater treatment system.

    abstract:AIMS:To measure the microbial removal capacity of a small-scale hydroponics wastewater treatment plant. METHODS AND RESULTS:Paired samples were taken from untreated, partly-treated and treated wastewater and analysed for faecal microbial indicators, i.e. coliforms, Escherichia coli, enterococci, Clostridium perfringen...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2005.01689.x

    authors: Ottoson J,Norström A,Dalhammar G

    更新日期:2005-01-01 00:00:00

  • Detection and characterization of the ferric uptake regulator (fur) gene in Plesiomonas shigelloides.

    abstract::Iron availability plays an important role in the virulence of micro-organisms, which develops different systems for iron acquisition. The expression of genes involved in iron uptake systems is usually regulated by Fur, a transcriptional regulator. Plesiomonas shigelloides is a Gram-negative food- and water-borne enter...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12858

    authors: Rodríguez-Rodríguez S,Santos JA

    更新日期:2018-04-01 00:00:00

  • The solubility of the ribotoxin alpha-sarcin, produced as a recombinant protein in Escherichia coli, is increased in the presence of thioredoxin.

    abstract::The yield of purified recombinant alpha-sarcin increases approximately three- to fourfold when this toxin is co-expressed in Escherichia coli with thioredoxin. This increased production is attributed to the existence, in the presence of thioredoxin, of a reducing environment which allows rearrangement of incorrect dis...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2000.00714.x

    authors: García-Ortega L,Lacadena J,Lacadena V,Masip M,De Antonio C,Martínez-Ruiz A,Martínez Del Pozo A

    更新日期:2000-04-01 00:00:00

  • Evaluation of a multiplex PCR assay for simultaneous identification of Salmonella sp., Salmonella enteritidis and Salmonella typhimurium from environmental swabs of poultry houses.

    abstract::A Multiplex PCR-based assay (m-PCR) with three sets of primers was developed for the detection of all serotypes of Salmonella enterica and the identification of Salmonella Enteritidis and Salmonella Typhimurium. This method was evaluated against a bacteriological method for the analysis of environmental swabs of poult...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00488.x

    authors: Soumet C,Ermel G,Rose N,Rose V,Drouin P,Salvat G,Colin P

    更新日期:1999-02-01 00:00:00

  • Comparison of the acidifying activity of Lactococcus lactis subsp. lactis strains isolated from goat's milk and Valdeteja cheese.

    abstract:AIMS:This work was carried out to study the acid production by Lactococcus lactis subsp. lactis strains isolated from goat's milk and goat cheese (Valdeteja variety) in order to select a suitable starter culture for industrial goat cheese manufacturing. METHODS AND RESULTS:The titrable acidity of 45 Lactococcus lactis...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01061.x

    authors: Alonso-Calleja C,Carballo J,Capita R,Bernardo A,García-López ML

    更新日期:2002-01-01 00:00:00

  • Incidence of Campylobacter and Salmonella isolated from retail chicken and associated packaging in South Wales.

    abstract:AIMS:To investigate the incidence of Campylobacter and Salmonella contamination associated with supermarket and butchers' shop chicken and related packaging. METHOD AND RESULTS:Three hundred raw samples (whole chicken, chicken breast with skin or chicken pieces) were purchased on a monthly basis for seven months. Pack...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2001.01031.x

    authors: Harrison WA,Griffith CJ,Tennant D,Peters AC

    更新日期:2001-12-01 00:00:00

  • Novel ecological niche of Cetobacterium somerae, an anaerobic bacterium in the intestinal tracts of freshwater fish.

    abstract:AIMS:This study was conducted to clarify the taxonomic status of Bacteroides type A strains with high vitamin B(12)-producing ability that is widely distributed in the intestinal tracts of freshwater fish. METHODS AND RESULTS:Seventeen strains of Bacteroides type A isolated from five fish species were all rod-shaped a...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02258.x

    authors: Tsuchiya C,Sakata T,Sugita H

    更新日期:2008-01-01 00:00:00

  • Evaluation of the BBL Crystal Enteric/Nonfermenter kit for the identification of water-derived environmental Enterobacteriaceae.

    abstract::The Crystal Enteric/Nonfermenter (E/NF) identification kit (Becton Dickinson Microbiology Systems, USA) was evaluated using water-derived bacterial isolates and results compared to those obtained by the API 20E system (BioMérieux, UK). Both the E/NF and 20E systems correctly identified 93% of the Enterobacteriaceae re...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1995.tb01031.x

    authors: Micklewright IJ,Sartory DP

    更新日期:1995-09-01 00:00:00

  • Survival of Salmonella enteritidis Pt4 and Salm. typhimurium Swindon in aerosols.

    abstract::Small particle aerosols of plate-grown Salmonella enteritidis and Salm. typhimurium were generated and maintained within a rotating drum at 75% relative humidity and 24 degrees C for 2 h. Plate-grown organisms were found to be more aerosol-stable than broth-grown organisms. Differences were observed between the two sp...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1996.tb00042.x

    authors: McDermid AS,Lever MS

    更新日期:1996-08-01 00:00:00

  • Typing clinical and animal environment Aspergillus fumigatus gliotoxin producer strains isolated from Brazil by PCR-RFLP markers.

    abstract:UNLABELLED:Aspergillus fumigatus, a well-known human and animal pathogen causing aspergillosis, has been historically identified by morphological and microscopic features. However, recent studies have shown that species identification on the basis of morphology alone is problematic. The aim of this work was to confirm ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12136

    authors: Soleiro CA,Pena GA,Cavaglieri LR,Coelho I,Keller LM,Dalcero AM,Rosa CA

    更新日期:2013-12-01 00:00:00

  • Comparative phytochemical analysis and antibacterial efficacy of in vitro and in vivo extracts from East Indian sandalwood tree (Santalum album L.).

    abstract:UNLABELLED:Sandalwood oil has been found in numerous therapeutic applications in traditional medicines such as Chinese traditional medicine and Ayurveda. However, there are no comparative accounts available in the literature that focused on in vitro and in vivo tree sample-derived extracts. Combined dichloromethane and...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12005

    authors: Misra BB,Dey S

    更新日期:2012-12-01 00:00:00

  • Flow cytometric analysis of Lactobacillus plantarum to monitor lag times, cell division and injury.

    abstract::Flow cytometry in combination with fluorescent molecular markers 5- (and 6-) carboxyfluorescein succinimidylester (CFSE) and propidium iodide (PI) have been applied to determine lag times, numbers of cell divisions and injury after mild heat (50 degrees C, 5 min) and nisin treatments (0.1 and 1.0 microgram ml-1) of La...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1997.00225.x

    authors: Ueckert JE,Nebe von-Caron G,Bos AP,ter Steeg PF

    更新日期:1997-10-01 00:00:00

  • Characterization of dextran-producing Leuconostoc strains.

    abstract::Leuconostoc strains were characterized according to their antibiotic susceptibilities, carbohydrate fermentation profiles, sucrase activity patterns and plasmid content. All the strains tested were resistant to the antibiotics sulphathiazole, trimethoprim and vancomycin, and could be separated into two groups based on...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2001.00891.x

    authors: Holt SM,Al-Sheikh H,Shin KJ

    更新日期:2001-03-01 00:00:00

  • Silica-immobilized Methylobacterium sp. NP3 and Acinetobacter sp. PK1 degrade high concentrations of phenol.

    abstract:AIMS:To immobilize Methylobacterium sp. NP3 and Acinetobacter sp. PK1 to silica and determine the ability of the immobilized bacteria to degrade high concentrations of phenol. METHODS AND RESULTS:The phenol degradation activity of suspended and immobilized Methylobacterium sp. NP3 and Acinetobacter sp. PK1 bacteria wa...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2011.03019.x

    authors: Khongkhaem P,Intasiri A,Luepromchai E

    更新日期:2011-05-01 00:00:00

  • Efficacy of biocides on laboratory-generated Legionella biofilms.

    abstract::Results of biocide efficacy testing on laboratory-generated microbial biofilms containing Legionella bozemanii is presented. These show that chlorine is effective at recommended concentrations in cooling towers; at least 48 h contact between free chlorine and the biofilm is necessary. Of five commercial biocides teste...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1993.tb00384.x

    authors: Green PN

    更新日期:1993-10-01 00:00:00

  • Decontamination of dental unit water systems with hydrogen peroxide.

    abstract:AIMS:Transmission of microbial pathogens to patients from water in dental units is a concern. To reduce this risk, the decontaminating efficiency of hydrogen peroxide was evaluated. METHODS AND RESULTS:Three percent hydrogen peroxide diluted 1 : 4 in distilled water (contact time 15 min) was used daily to disinfect th...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01378.x

    authors: Zanetti F,De Luca G,Tarlazzi P,Stampi S

    更新日期:2003-01-01 00:00:00

  • In vitro efficacy of the novel peptide CECT7121 against bacteria isolated from mastitic dairy cattle.

    abstract:AIMS:To evaluate the in vitro bactericidal activity of the novel antimicrobial peptide (AP) CECT7121 against Gram-positive bacteria from mastitic dairy cattle. METHODS AND RESULTS:A total of 15 Staphylococcus aureus, 10 Streptococcus dysgalactiae, 7 Strep. uberis, 1 Strep. agalactiae strains were isolated from 33 diff...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02507.x

    authors: Sparo MD,Jones DG,Sánchez Bruni SF

    更新日期:2009-02-01 00:00:00

  • Detection and quantification of Aspergillus ochraceus in green coffee by PCR.

    abstract:AIMS:The aim of this study was to detect and quantify DNA of the ochratoxinogenic fungus Aspergillus ochraceus in green coffee and to compare the results with the ochratoxin A content of naturally contaminated samples. METHODS AND RESULTS:A DNA extraction protocol based on a combination of ultrasonification and a comm...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2004.01524.x

    authors: Schmidt H,Bannier M,Vogel RF,Niessen L

    更新日期:2004-01-01 00:00:00

  • Identification and characterization of a new three-component nicotinic acid hydroxylase NahAB1 B2 from Pusillimonas sp. strain T2.

    abstract::Nicotinic acid (NA) is ubiquitous in nature and its microbial degradation mechanisms are diverse. In this study, Pusillimonas sp. strain T2 was found to be capable of utilizing NA as sole carbon and nitrogen sources. This strain could completely degrade 300 mg l-1 NA within 3·5 h at 30°C and pH 7·0 and one of the degr...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12850

    authors: Yuan M,Zhang Y,Zhao L,Ma Y,He Q,He J,Qiu J

    更新日期:2018-04-01 00:00:00

  • Genetic heterogeneity of non-O1 and non-O139 Vibrio cholerae isolates from shrimp aquaculture system: a comparison of RS-, REP- and ERIC-PCR fingerprinting approaches.

    abstract:AIMS:The genetic diversity of Vibrio cholerae isolated from black tiger shrimp (Penaeus monodon) aquaculture farms was determined using three PCR typing methods based on enterobacterial repetitive intergenic consensus (ERIC) sequences, ribosomal gene spacer (RS) sequence and repetitive extragenic palindromic (REP) sequ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2010.02863.x

    authors: Rao BM,Surendran PK

    更新日期:2010-07-01 00:00:00

  • Microbial transformation of hydroxy metabolites of 1-oxohexyl derivatives of theobromine by Cunninghamella echinulata NRRL 1384.

    abstract:AIM:The biotransformation of pentoxifylline (PTX), propentofylline (PPT) and their racemic hydroxy metabolites ((+/-)-OHPTX and (+/-)-OHPPT) by using the fungus Cunninghamella echinulata NRRL 1384. METHODS AND RESULTS:A fungus Cunninghamella echinulata NRRL 1384 was used to catalyse the (S)-selective oxidation of the ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02478.x

    authors: Pekala E,Kochan M,Carnell AJ

    更新日期:2009-01-01 00:00:00

  • Environmental factors influencing the inactivation of Listeria monocytogenes by pulsed electric fields.

    abstract:AIMS:To investigate the influence of the growth phase, growth temperature, storage time, pH and aw of the treatment medium on the resistance of Listeria monocytogenes to pulsed electric fields (PEF). METHODS AND RESULTS:Square wave pulses of 2 micros at a frequency of 1 Hz and 25 and 28 kV cm(-1) were used. Cells were...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01221.x

    authors: Alvarez I,Pagán R,Raso J,Condón S

    更新日期:2002-01-01 00:00:00

  • Characterization and molecular cloning of Bifidobacterium longum cryptic plasmid pMB1.

    abstract::The small cryptic plasmid pMB1 (1.9 kb), previously isolated from Bifidobacterium longum, has been characterized by physical mapping. Two cloning vectors, pMR3 and pDG7, carrying chloramphenicol and ampicillin resistances derived from pJH101, have been electroporated in Escherichia coli. ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1990.tb00165.x

    authors: Matteuzzi D,Brigidi P,Rossi M,Di D

    更新日期:1990-10-01 00:00:00