Abstract:
:Fattening pigs are often infected with campylobacter. To eliminate campylobacter from the pig population, a top-down approach, involving the breeding and reproduction farms, seems appropriate. In order to investigate the effectiveness of a top-down approach, sows' faeces from the following farms were analysed for the presence of campylobacter: one specific pathogen free (SPF) farm, three top-breeding farms with no connection with SPF breeding, and a breeding farm repopulated with SPF sows after a period of vacancy (farm 5). The faeces samples from the SPF farm were free from campylobacter. The three top-breeding farms provided faeces samples which were 98% positive for campylobacter. However, only 22% of the faeces samples from farm 5 were positive for campylobacter. In a period of 20 months, the percentage of sows infected with campylobacter on farm 5 did not significantly increase. Genetic typing with ERIC-PCR and RFLP of campylobacter isolates from one of the top-breeding farms and from farm 5 showed a high diversity of campylobacter types. The results suggest that a campylobacter-free pig population can be established in breeding farms by combining a top-down approach (campylobacter-free top-breeding farms) with a strict regime of hygiene management.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Weijtens MJ,Urlings HA,Van der Plas Jdoi
10.1046/j.1472-765x.2000.00750.xsubject
Has Abstractpub_date
2000-06-01 00:00:00pages
479-84issue
6eissn
0266-8254issn
1472-765Xpii
lam750journal_volume
30pub_type
杂志文章abstract:AIMS:To elucidate the molecular mechanisms associated with mycoparasitism from Chaetomium cupreum, an effective biocontrol agent with ability against plant pathogenic fungi. METHODS AND RESULTS:One cDNA library was constructed from conditions predicted to resemble mycoparasitic process. A total of 1876 ESTs were gener...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2008.02487.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01948.x
更新日期:2006-09-01 00:00:00
abstract::A wild type micro-organism producing antibacterial substances has been isolated from a Chinese fermented soybean seasoning and identified as Bacillus subtilis. A crude antibacterial preparation (CABP) was obtained by ammonium sulphate precipitation. Isoelectric focusing assay revealed at least four antimicrobial compo...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00545.x
更新日期:1999-05-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.00900.x
更新日期:2001-04-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12064
更新日期:2013-06-01 00:00:00
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02233.x
更新日期:2007-12-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12619
更新日期:2016-10-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1995.tb01031.x
更新日期:1995-09-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1995.tb00407.x
更新日期:1995-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01036.x
更新日期:2002-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2000.00653.x
更新日期:2000-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12850
更新日期:2018-04-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2000.00700.x
更新日期:2000-02-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01278.x
更新日期:2003-01-01 00:00:00
abstract:UNLABELLED:This study reports the development of a loop-mediated isothermal amplification (LAMP) reaction for the detection of Pythium myriotylum. The primer set targeting the ITS sequence of P. myriotylum worked most efficiently at 60°C and allowed the detection of P. myriotylum DNA within 30 min by fluorescence monit...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12244
更新日期:2014-07-01 00:00:00
abstract::Anti-MRSA activity of sophoraflavanone G (SFG) and synergism between SFG and antibacterial agents against MRSA (methicillin-resistant Staphylococcus aureus) were evaluated by means of Minimal Inhibitory Concentrations (MIC). The MICs of SFG against 27 strains of MRSA ranged from 3.13 to 6.25 micrograms ml-1. Synergism...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1998.00386.x
更新日期:1998-08-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01370.x
更新日期:2003-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01915.x
更新日期:2006-06-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1998.00087.x
更新日期:1998-02-01 00:00:00
abstract::Increasingly, molecular methods have become important in identification and confirmation of bacteria at the species level. Rapid molecular methods provide sensitivity and specificity while reducing cost and resources. The primary goal of this study was to develop a real-time PCR assay for identification of Escherichia...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12359
更新日期:2015-02-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02955.x
更新日期:2010-12-01 00:00:00
abstract:AIMS:The study aim was to determine the presence of total and faecal coliforms on kitchen surfaces, in tap water and on the hands of caregivers in households on both sides of the US-Mexico border. METHODS AND RESULTS:Samples were collected in 135 randomly selected households in Ciudad Juarez, Mexico, and El Paso, Texa...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2008.02378.x
更新日期:2008-06-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03101.x
更新日期:2011-09-01 00:00:00
abstract:AIMS:To characterize the bacterial communities in commercial total mixed ration (TMR) silage, which is known to have a long bunk life after silo opening. METHODS AND RESULTS:Samples were collected from four factories that produce TMR silage according to their own recipes. Three factories were sampled three times at 1-...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02915.x
更新日期:2010-10-01 00:00:00
abstract:AIMS:Overproduced alpha-amylases in Bacillus subtilis provoke a specific stress response involving the CssRS two-component system, which controls expression of the HtrA and HtrB proteases. Previously, the B. subtilis TepA protein was implicated in high-level alpha-amylase secretion. Our present studies were aimed at in...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01539.x
更新日期:2004-01-01 00:00:00
abstract::Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a worldwide concern. Cattle are their main reservoir and may contaminate watercourses through manure. We characterized a collection of 38 STEC O157:H7 strains isolated from surface water in feedlots areas (puddles inside pens formed after the rainfall or by spil...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12873
更新日期:2018-06-01 00:00:00
abstract::Suitable conditions for the introduction of bacteriophage DNA into cells of Rhodococcus rhodochrous CF222 by electroporation were established, and penicillin G was found to enhance the transfection frequency. When conditions optimal for the parental strain were applied to its colony-morphological mutants, different tr...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1996.tb01110.x
更新日期:1996-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2005.01689.x
更新日期:2005-01-01 00:00:00