Phenotypic and genotypic diversity of Genista saharae microsymbionts from the infra-arid region of Tunisia.

Abstract:

AIMS:Genista saharae, indigenous of Sahara, is a spontaneous shrub that plays an important ecological role for the preservation and fertility of poor and eroded soils. This legume has not been examined for its root nodule bacteria. The taxonomic diversity of bacteria from root nodules of G. saharae growing in the infra-arid region of Tunisia was investigated. METHODS AND RESULTS:A total of 28 bacterial strains isolated from root nodules of G. saharae grown in Tunisian soil were characterized using a polyphasic approach including phenotypic characteristics, PCR-RFLP of 16S rDNA and 16S rRNA gene sequencing. It was found that new isolates are diverse and affiliated to Ensifer (75%), Rhizobium (10%) and Phyllobacterium (15%). The Phyllobacterium isolates lacked the capacity for nodule formation on this plant. CONCLUSIONS:Genista saharae formed nodules with diverse rhizobia in Tunisian soils. Furthermore, our results support the presence of non-nodulating commensal strains (Phyllobacterium) in legumes nodule. SIGNIFICANCE AND IMPACT OF THE STUDY:This study is the first report on the characterization of G. saharae microsymbionts in Tunisia.

journal_name

Lett Appl Microbiol

authors

Mahdhi M,Nzoué A,Gueye F,Merabet C,de Lajudie P,Mars M

doi

10.1111/j.1472-765X.2007.02233.x

subject

Has Abstract

pub_date

2007-12-01 00:00:00

pages

604-9

issue

6

eissn

0266-8254

issn

1472-765X

pii

LAM2233

journal_volume

45

pub_type

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