Abstract:
AIM:To study genetic diversity of Chromobacterium haemolyticum isolates recovered from a natural tropical lake. METHODS AND RESULTS:A set of 31 isolates were recovered from a bacterial freshwater community by conventional plating methods and subjected to genetic and phenotypic characterization. The 16S ribosomal RNA (rRNA) gene phylogeny revealed that the isolates were related most closely with C. haemolyticum. In addition to the molecular data, our isolates exhibited strong β-haemolytic activity, were nonviolacein producers and utilized i-inositol, D-mannitol and D-sorbitol in contrast with the other known chromobacteria. Evaluation of the genetic diversity in the 16S rRNA gene, tRNA intergenic spacers (tDNA) and 16S-23S internal transcribed spacers (ITS) unveiled different levels of genetic heterogeneity in the population, which were also observed with repetitive extragenic palindromic (rep)-PCR genomic fingerprinting using the BOX-AR1 primer. tDNA- and ITS-PCR analyses were partially congruent with the 16S rRNA gene phylogeny. The isolates exhibited high resistance to β-lactamic antibiotics. CONCLUSION:The population genetic heterogeneity was revealed by 16S rRNA gene sequence, ITS and BOX-PCR analysis. SIGNIFICANCE AND IMPACT OF THE STUDY:This study provides for the first time an insight into the genetic diversity of phylogenetically close isolates to C. haemolyticum species.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Lima-Bittencourt CI,Costa PS,Barbosa FA,Chartone-Souza E,Nascimento AMdoi
10.1111/j.1472-765X.2011.03052.xsubject
Has Abstractpub_date
2011-06-01 00:00:00pages
642-50issue
6eissn
0266-8254issn
1472-765Xjournal_volume
52pub_type
杂志文章abstract::Ergosterol biosynthesis in Saccharomyces cerevisiae is complex and the underlying mechanism of regulation remains unclear. To clarify the influence of transcriptional regulation on the ergosterol content, transcription factor Ecm22 was overexpressed in S. cerevisiae. Results showed that the overexpression of ECM22 led...
journal_title:Letters in applied microbiology
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doi:10.1111/lam.13061
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abstract:AIMS:In this study, we compared different methods of colistin susceptibility testing, disc diffusion, agar dilution and Etest using a set of Enterobacteriaceae isolates that included colistin-resistant strains. METHODS AND RESULTS:Susceptibility of 200 clinical isolates of Enterobacteriaceae to colistin was tested to ...
journal_title:Letters in applied microbiology
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doi:10.1111/j.1472-765X.2011.03145.x
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journal_title:Letters in applied microbiology
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doi:10.1046/j.1472-765x.2002.01036.x
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doi:10.1111/j.1472-765X.2004.01590.x
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1998.00358.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1995.tb01079.x
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journal_title:Letters in applied microbiology
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doi:10.1111/j.1472-765X.2011.03076.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2000.00742.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02302.x
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02785.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01047.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02583.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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journal_title:Letters in applied microbiology
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更新日期:2012-02-01 00:00:00
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journal_title:Letters in applied microbiology
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更新日期:2014-10-01 00:00:00
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journal_title:Letters in applied microbiology
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更新日期:2018-11-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1998.00406.x
更新日期:1998-10-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01273.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:Overproduced alpha-amylases in Bacillus subtilis provoke a specific stress response involving the CssRS two-component system, which controls expression of the HtrA and HtrB proteases. Previously, the B. subtilis TepA protein was implicated in high-level alpha-amylase secretion. Our present studies were aimed at in...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01539.x
更新日期:2004-01-01 00:00:00
abstract::A strain named as Pseudomonas aeruginosa 2016NX1, which could produce phenazine and cereusitin, was isolated from the root of Millettia specisoa. Phenazines were extracted, isolated and purified by chloroform, thin-layer chromatography, column chromatography and high-performance liquid chromatography. Then the purifie...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13332
更新日期:2020-09-01 00:00:00
abstract:AIMS:To evaluate mycobiota and aflatoxins B(1) (AFB(1)), B(2) (AFB(2)), G(1) (AFG(1)), G(2) (AFG(2)) and fumonisin B(1) (FB(1)) contamination in different malted barley types and brands and brewer's grain collected from a major Argentinean brewery. METHODS AND RESULTS:Total fungal counts were performed using the plate...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03157.x
更新日期:2011-12-01 00:00:00
abstract:UNLABELLED:Most of the current research into the quantification of soil-borne pathogenic oomycetes lacks determination of DNA extraction efficiency, probably leading to an incorrect estimation of DNA quantity. In this study, we developed a convenient method by using a 100 bp artificially synthesized DNA sequence derive...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12441
更新日期:2015-08-01 00:00:00