Growth of Salmonella on sprouting alfalfa seeds as affected by the inoculum size, native microbial load and Pseudomonas fluorescens 2-79.

Abstract:

AIMS:To investigate the growth of salmonellae on sprouting alfalfa seeds as affected by the inoculum size, microbial load and Pseudomonas fluorescens 2-79. METHODS AND RESULTS:Alfalfa seeds pre-inoculated with < or =10(1)-10(3) CFU g(-1) of salmonellae and with or without Ps. fluorescens 2-79 were sprouted in glass jars and the population of salmonellae were determined daily for up to 6 days. The population of salmonellae on germinating seeds reached the maximum 2-3 days after sprouting when total bacterial count reached the maximum (10(9) CFU g(-1)). The population of salmonellae on sprouting seeds not treated with Ps. fluorescens 2-79 showed a net increase of 3-4 log units. However, the population of salmonellae on alfalfa seeds treated with Ps. fluorescens 2-79 showed a net increase of only 1-2 log units. Disinfection of seeds with calcium hypochlorite enhanced the growth of salmonellae. CONCLUSIONS:Treatment of seeds with Ps. fluorescens 2-79 reduced the growth of salmonellae by 2-3 log units. SIGNIFICANCE AND IMPACT OF THE STUDY:The potential of Ps. fluorescens 2-79 as a biological agent for use in control of salmonellae on sprouting seeds was demonstrated and warrants further investigation.

journal_name

Lett Appl Microbiol

authors

Liao CH

doi

10.1111/j.1472-765X.2007.02302.x

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

232-6

issue

2

eissn

0266-8254

issn

1472-765X

pii

LAM2302

journal_volume

46

pub_type

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