Growth of Salmonella on sprouting alfalfa seeds as affected by the inoculum size, native microbial load and Pseudomonas fluorescens 2-79.

Abstract:

AIMS:To investigate the growth of salmonellae on sprouting alfalfa seeds as affected by the inoculum size, microbial load and Pseudomonas fluorescens 2-79. METHODS AND RESULTS:Alfalfa seeds pre-inoculated with < or =10(1)-10(3) CFU g(-1) of salmonellae and with or without Ps. fluorescens 2-79 were sprouted in glass jars and the population of salmonellae were determined daily for up to 6 days. The population of salmonellae on germinating seeds reached the maximum 2-3 days after sprouting when total bacterial count reached the maximum (10(9) CFU g(-1)). The population of salmonellae on sprouting seeds not treated with Ps. fluorescens 2-79 showed a net increase of 3-4 log units. However, the population of salmonellae on alfalfa seeds treated with Ps. fluorescens 2-79 showed a net increase of only 1-2 log units. Disinfection of seeds with calcium hypochlorite enhanced the growth of salmonellae. CONCLUSIONS:Treatment of seeds with Ps. fluorescens 2-79 reduced the growth of salmonellae by 2-3 log units. SIGNIFICANCE AND IMPACT OF THE STUDY:The potential of Ps. fluorescens 2-79 as a biological agent for use in control of salmonellae on sprouting seeds was demonstrated and warrants further investigation.

journal_name

Lett Appl Microbiol

authors

Liao CH

doi

10.1111/j.1472-765X.2007.02302.x

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

232-6

issue

2

eissn

0266-8254

issn

1472-765X

pii

LAM2302

journal_volume

46

pub_type

杂志文章
  • Optimization of fermentation conditions for the expression of sweet-tasting protein brazzein in Lactococcus lactis.

    abstract:AIMS:To improve the production of sweet-tasting protein brazzein in Lactococcus lactis using controlled fermentation conditions. METHODS AND RESULTS:The nisin-controlled expression system was used for brazzein expression. The concentration of nisin for induction and the optical density (OD) at induction were therefore...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02297.x

    authors: Berlec A,Tompa G,Slapar N,Fonović UP,Rogelj I,Strukelj B

    更新日期:2008-02-01 00:00:00

  • Decontamination of dental unit water systems with hydrogen peroxide.

    abstract:AIMS:Transmission of microbial pathogens to patients from water in dental units is a concern. To reduce this risk, the decontaminating efficiency of hydrogen peroxide was evaluated. METHODS AND RESULTS:Three percent hydrogen peroxide diluted 1 : 4 in distilled water (contact time 15 min) was used daily to disinfect th...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01378.x

    authors: Zanetti F,De Luca G,Tarlazzi P,Stampi S

    更新日期:2003-01-01 00:00:00

  • Diversity of bacterial capabilities in utilizing alkylated benzenes and other aromatic compounds.

    abstract::Seventeen aerobic bacterial strains known to degrade one or more aromatic compounds were tested for their ability to utilize benzene, seven different methylated benzenes or other aromatic compounds as their sole source of carbon. The results are discussed with respect to the possibility of finding strains which degrad...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1996.tb00078.x

    authors: Lang E

    更新日期:1996-10-01 00:00:00

  • Divercin V41 from gene characterization to food applications: 1998-2008, a decade of solved and unsolved questions.

    abstract::The emergence of an increasing number of antibiotic resistant human clinical bacteria has been a great cause of concern for the last decades. As an example, Staphylococcus aureus isolates in the hospital environment are becoming more and more resistant to antibiotics including vancomycin which is considered as a last ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1472-765X.2008.02490.x

    authors: Rihakova J,Belguesmia Y,Petit VW,Pilet MF,Prévost H,Dousset X,Drider D

    更新日期:2009-01-01 00:00:00

  • Enhancement of paraben-fungicidal activity by sulforaphane, a cruciferous vegetable-derived isothiocyanate, via membrane structural damage in Saccharomyces cerevisiae.

    abstract::Parabens have been widely used as antimicrobial preservatives in cosmetics, pharmaceuticals, foods and beverages. Commonly, methyl-, ethyl-, propyl- and butylparaben are used independently or in combination to maintain the quality of industrial products, and they are considered to have low toxicity. However, recent ev...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13230

    authors: Murata W,Yamaguchi Y,Fujita KI,Yamauchi K,Tanaka T,Ogita A

    更新日期:2019-12-01 00:00:00

  • Enhancing the fungicidal activity of amphotericin B via vacuole disruption by benzyl isothiocyanate, a cruciferous plant constituent.

    abstract::Amphotericin B (AmB), a typical polyene macrolide antifungal agent, is widely used to treat systemic mycoses. In the present study, we show that the fungicidal activity of AmB was enhanced by benzyl isothiocyanate (BITC), a cruciferous plant-derived compound, in the budding yeast, Saccharomyces cerevisiae. In addition...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13425

    authors: Yamada N,Murata W,Yamaguchi Y,Fujita KI,Ogita A,Tanaka T

    更新日期:2020-10-31 00:00:00

  • Draft genome sequence of Streptomyces coelicoflavus ZG0656 reveals the putative biosynthetic gene cluster of acarviostatin family α-amylase inhibitors.

    abstract:AIMS:The aims of this study are to obtain the draft genome sequence of Streptomyces coelicoflavus ZG0656, which produces novel acarviostatin family α-amylase inhibitors, and then to reveal the putative acarviostatin-related gene cluster and the biosynthetic pathway. METHODS AND RESULTS:The draft genome sequence of S. ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2012.03274.x

    authors: Guo X,Geng P,Bai F,Bai G,Sun T,Li X,Shi L,Zhong Q

    更新日期:2012-08-01 00:00:00

  • Design of a species-specific PCR method for the detection of the heat-resistant fungi Talaromyces macrosporus and Talaromyces trachyspermus.

    abstract::Heat-resistant fungi occur sporadically and are a continuing problem for the food and beverage industry. The genus Talaromyces, as a typical fungus, is capable of producing the heat-resistant ascospores responsible for the spoilage of processed food products. Isocitrate lyase, a signature enzyme of the glyoxylate cycl...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12818

    authors: Yamashita S,Nakagawa H,Sakaguchi T,Arima TH,Kikoku Y

    更新日期:2018-01-01 00:00:00

  • Progesterone transformation as a diagnostic feature in the classification of the Aspergillus niger group.

    abstract::Fifty-five isolates of nine species and two species varieties (5 isolates each) of the Aspergillus niger group were tested for progesterone transformation. Generally, all isolates of the different species transformed progesterone to 21-hydroxyprogesterone. In addition, the isolates of seven species have the capacity t...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1995.tb00438.x

    authors: Mostafa ME

    更新日期:1995-04-01 00:00:00

  • Viability of zoonotic pathogens Escherichia coli and Salmonella in swine manure slurries with and without a urease inhibitor and thymol.

    abstract:AIMS:To determine the effects of urine, a urease inhibitor and/or an odour-reducing antimicrobial compound, on zoonotic pathogens in swine manure slurries. METHODS AND RESULTS:Swine faeces were collected and blended with different amounts of urine. Marker strains of Salmonella enterica subsp. enterica serovar Typhimur...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02338.x

    authors: Wells JE,Varel VH

    更新日期:2008-04-01 00:00:00

  • An investigation into the ecological niches and seasonal nature of Clostridium estertheticum and Clostridium gasigenes in the Irish beef farm environment.

    abstract::Blown pack spoilage (BPS) of vacuum packaged beef is caused by psychrotolerant and psychrophilic Clostridium species, primarily Clostridium estertheticum and Clostridium gasigenes. The aim of this study was to investigate the environmental niches and impact of season on these BPS Clostridium spp. on Irish beef farms. ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13344

    authors: Esteves E,Whyte P,Gupta TB,Bolton DJ

    更新日期:2020-12-01 00:00:00

  • Bidirectional transformation of aromatic aldehydes by Desulfovibrio desulfuricans under nitrate-dissimilating conditions.

    abstract::Desulfovibrio desulfuricans ATCC 27774 was screened for reactivity against aromatic compounds during lactate-dependent, nitrate-dissimilating growth. Only aromatic aldehydes (benzaldehyde, 2-hydroxybenzaldehyde, 3-hydroxybenzaldehyde, 4-hydroxybenzaldehyde, vanillin, iso-vanillin and o-vanillin) were reactive and, wit...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1996.tb01122.x

    authors: Parekh M,Drake HL,Daniel SL

    更新日期:1996-02-01 00:00:00

  • Molasses supplementation promotes conidiation but suppresses aflatoxin production by small sclerotial Aspergillus flavus.

    abstract:AIMS:To find a supplemental ingredient that can be added to routinely used growth media to increase conidial production and decrease aflatoxin biosynthesis in small sclerotial (S strain) isolates of Aspergillus flavus. METHODS AND RESULTS:Molasses was added to three commonly used culture media: coconut agar (CAM), pot...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.02056.x

    authors: Chang PK,Hua SS

    更新日期:2007-02-01 00:00:00

  • Inactivation of Salmonella enterica serovar enteritidis in shell eggs by sequential application of heat and ozone.

    abstract:AIMS:To assess the contribution of ozone to lethality of Salmonella enterica serovar Enteritidis in experimentally inoculated whole shell eggs that are sequentially treated with heat and gaseous ozone in pilot-scale equipment. METHODS AND RESULTS:Whole shell eggs were inoculated with small populations of Salmonella En...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02367.x

    authors: Perry JJ,Rodriguez-Romo LA,Yousef AE

    更新日期:2008-06-01 00:00:00

  • Flow cytometric analysis of Lactobacillus plantarum to monitor lag times, cell division and injury.

    abstract::Flow cytometry in combination with fluorescent molecular markers 5- (and 6-) carboxyfluorescein succinimidylester (CFSE) and propidium iodide (PI) have been applied to determine lag times, numbers of cell divisions and injury after mild heat (50 degrees C, 5 min) and nisin treatments (0.1 and 1.0 microgram ml-1) of La...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1997.00225.x

    authors: Ueckert JE,Nebe von-Caron G,Bos AP,ter Steeg PF

    更新日期:1997-10-01 00:00:00

  • Sensitivity of nisin-resistant Listeria monocytogenes to heat and the synergistic action of heat and nisin.

    abstract::Nisin, a bacteriocin produced by some strains of Lactococcus lactis, acts against foodborne pathogen Listeria monocytogenes. A single exposure of cells to nisin can generate nisin-resistant (Nisr) mutants, which may compromise the use of nisin in the food industry. The objective of this research was to compare the hea...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2000.00708.x

    authors: Modi KD,Chikindas ML,Montville TJ

    更新日期:2000-03-01 00:00:00

  • Nanobacteria--propagating calcifying nanoparticles.

    abstract::Nanobacteria, also known as calcifying nanoparticles (CNP), are controversial infectious agents not matching the current criteria for 'living organism'. Despite the controversy of their classification, they propagate and cause cell death in vitro and are associated or found in many human diseases. Thus, more efforts s...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1472-765X.2006.01945.x

    authors: Kajander EO

    更新日期:2006-06-01 00:00:00

  • Technical advance in fungal biotechnology: development of a miniaturized culture method and an automated high-throughput screening.

    abstract:AIMS:The goal of the study was to develop a reliable, reproducible and rapid method of culture in order to screen a large number of fungal transformants. METHODS AND RESULTS:The method is based upon miniaturized cell cultures and automated expression screening in microwell plates. For the method development, 50 recomb...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2009.02655.x

    authors: Alberto F,Navarro D,de Vries RP,Asther M,Record E

    更新日期:2009-08-01 00:00:00

  • Detection and discrimination of four Aspergillus section Nigri species by PCR.

    abstract::Species of Aspergillus section Nigri are not easily distinguished by traditional morphological techniques, and typically are identified by DNA sequencing methods. We developed four PCR primers to distinguish between Aspergillus niger, Aspergillus welwitschiae, Aspergillus carbonarius and Aspergillus tubingensis, based...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12358

    authors: Palumbo JD,O'Keeffe TL

    更新日期:2015-02-01 00:00:00

  • Detection of live Salmonella enterica in fresh-cut vegetables by a TaqMan-based one-step reverse transcription real-time PCR.

    abstract::Fresh-cut produce is at greater risk of Salmonella contamination. Detection and early warning systems play an important role in reducing the dissemination of contaminated products. One-step Reverse Transcription Polymerase Chain Reaction (RT-qPCR) targeting Salmonella tmRNA with or without a 6-h enrichment was evaluat...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12871

    authors: Miao YJ,Xiong GT,Bai MY,Ge Y,Wu ZF

    更新日期:2018-05-01 00:00:00

  • Detection and potential indicators of the presence of hepatitis C virus on surfaces in hospital settings.

    abstract:AIMS:The risk of hepatitis C virus infection in hospital environments can be assessed not only by studying epidemiological data and work practices, but also by the detection of these viruses (or indicators thereof) in health-care settings, on instruments etc. METHODS:Since standardized techniques specific to this end ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01066.x

    authors: Carducci A,Verani M,Casini B,Giuntini A,Mazzoni F,Rovini E,Passaglia A,Giusti L,Valenza A,Lombardi R

    更新日期:2002-01-01 00:00:00

  • Studies of the release of small molecules during pressure germination of spores of Bacillus subtilis.

    abstract:AIMS:To measure rates of release of small molecules during pressure germination of Bacillus subtilis spores, and the role of SpoVA proteins in dipicolinic acid (DPA) release. METHODS AND RESULTS:Rates of DPA release during B. subtilis spore germination with pressures of 150 or 500 megaPascals were much higher in spore...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02204.x

    authors: Vepachedu VR,Hirneisen K,Hoover DG,Setlow P

    更新日期:2007-09-01 00:00:00

  • Rapid screening astaxanthin-hyperproducing Haematococcus pluvialis mutants through near-infrared spectroscopy.

    abstract:UNLABELLED:The unicellular freshwater green microalga Haematococcus pluvialis is the richest source of natural astaxanthin. Since accumulation of astaxanthin differs significantly among various algal strains at different stages, it is therefore critical to develop an effective high-throughput assay for rapid screening ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12531

    authors: Liu JH,Song L,Huang Q

    更新日期:2016-02-01 00:00:00

  • Overexpression of Ecm22 improves ergosterol biosynthesis in Saccharomyces cerevisiae.

    abstract::Ergosterol biosynthesis in Saccharomyces cerevisiae is complex and the underlying mechanism of regulation remains unclear. To clarify the influence of transcriptional regulation on the ergosterol content, transcription factor Ecm22 was overexpressed in S. cerevisiae. Results showed that the overexpression of ECM22 led...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13061

    authors: Wang SQ,Wang T,Liu JF,Deng L,Wang F

    更新日期:2018-11-01 00:00:00

  • Utilization of chicory roots for microbial endoinulinase production.

    abstract:AIMS:The optimal culture conditions for endoinulinase production using chicory roots were studied in shake-flask culture. METHODS AND RESULTS:Much higher enzyme production was achieved with Xanthomonas sp. (15 U ml(-1)) than with Pseudomonas sp. (3 U ml(-1)). Optimized culture conditions of Xanthomonas sp. for endoinu...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2001.00977.x

    authors: Park JP,Yun JW

    更新日期:2001-09-01 00:00:00

  • Rapid construction of Campylobacter jejuni deletion mutants.

    abstract:AIMS:To develop a novel method for rapid construction of Campylobacter jejuni deletion mutants. METHODS AND RESULTS:We used overlapping extension PCR protocol to amplify a target sequence region of Camp. jejuni genomic DNA in which an internal fragment, Cj0618 coding sequence, was replaced by a chloramphenicol resista...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02232.x

    authors: Hansen CR,Khatiwara A,Ziprin R,Kwon YM

    更新日期:2007-12-01 00:00:00

  • Strains of Mesorhizobium amorphae and Mesorhizobium tianshanense, carrying symbiotic genes of common chickpea endosymbiotic species, constitute a novel biovar (ciceri) capable of nodulating Cicer arietinum.

    abstract:AIMS:To identify several strains of Mesorhizobium amorphae and Mesorhizobium tianshanense nodulating Cicer arietinum in Spain and Portugal, and to study the symbiotic genes carried by these strains. METHODS AND RESULTS:The sequences of 16S-23S intergenic spacer (ITS), 16S rRNA gene and symbiotic genes nodC and nifH we...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.02086.x

    authors: Rivas R,Laranjo M,Mateos PF,Oliveira S,Martínez-Molina E,Velázquez E

    更新日期:2007-04-01 00:00:00

  • Hydrolytic properties of crude alpha-L-rhamnosidases produced by several wild strains of mesophilic fungi.

    abstract:AIMS:Observation of the dependence of alpha-L-rhamnosidase activity on pH and temperature and the capability to hydrolyse concentrated naringin solutions and hesperidin suspensions of enzyme complexes produced by several fungi. METHODS AND RESULTS:The enzymes were produced by several wild strains of mesophilic fungi g...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01115.x

    authors: Scaroni E,Cuevas C,Carrillo L,Ellenrieder G

    更新日期:2002-01-01 00:00:00

  • Yarrowia lipolytica lipase production enhanced by increased air pressure.

    abstract:AIMS:To study the cellular growth and morphology of Yarrowia lipolytica W29 and its lipase and protease production under increased air pressures. METHODS AND RESULTS:Batch cultures of the yeast were conducted in a pressurized bioreactor at 4 and 8 bar of air pressure and the cellular behaviour was compared with cultur...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02299.x

    authors: Lopes M,Gomes N,Gonçalves C,Coelho MA,Mota M,Belo I

    更新日期:2008-02-01 00:00:00

  • Antagonists of bacitracin.

    abstract::Three groups of substances were identified which inhibit the bactericidal activity of bacitracin. Beside two divalent metals, Mg2+ and Ca2+, and a metabolite with chelating properties, citrate, the most pronounced effect was observed with pyrophosphate. Metals probably prevent access of bacitracin to the lipid carrier...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1994.tb00916.x

    authors: Podlesek Z,Comino A

    更新日期:1994-08-01 00:00:00