Hydrolytic properties of crude alpha-L-rhamnosidases produced by several wild strains of mesophilic fungi.

Abstract:

AIMS:Observation of the dependence of alpha-L-rhamnosidase activity on pH and temperature and the capability to hydrolyse concentrated naringin solutions and hesperidin suspensions of enzyme complexes produced by several fungi. METHODS AND RESULTS:The enzymes were produced by several wild strains of mesophilic fungi grown in liquid media containing rhamnose as sole carbon source. The properties and their ranges of values measured were as follows: (i) optimum pH, 3.5-6.5; (ii) optimum temperature, 50-65 degrees C; (iii) hydrolysis of supersaturated 100 g l(-1) naringin solutions, 45-100% and (iv) hydrolysis of hesperidin suspensions, 6-35%. CONCLUSIONS:Some alpha-L-rhamnosidase enzymes hydrolysed supersaturated naringin solutions with a high yield. The enzyme produced by Fusarium sambucinum 310 showed good activity even at pH 10. SIGNIFICANCE AND IMPACT OF THE STUDY:Crude enzymes with possible utilization as catalysts for the manufacture of hydrolysis products of the flavonoid glycosides were found.

journal_name

Lett Appl Microbiol

authors

Scaroni E,Cuevas C,Carrillo L,Ellenrieder G

doi

10.1046/j.1472-765x.2002.01115.x

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

461-5

issue

6

eissn

0266-8254

issn

1472-765X

pii

1115

journal_volume

34

pub_type

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