Abstract:
AIMS:To characterize the bacterial communities in commercial total mixed ration (TMR) silage, which is known to have a long bunk life after silo opening. METHODS AND RESULTS:Samples were collected from four factories that produce TMR silage according to their own recipes. Three factories were sampled three times at 1-month intervals during the summer to characterize the differences between factories; one factory was sampled 12 times, three samples each during the summer, autumn, winter and spring, to determine seasonal changes. Bacterial communities were determined by culture-independent denaturing gradient gel electrophoresis. All silages contained lactic acid as the predominant acid, and the contents appeared stable regardless of factories and product seasons. Acetic acid and 1-propanol contents were different between factories and indicated seasonal changes, with increases in warm seasons compared to cool seasons. Both differences and similarities existed among the bacterial communities from each factory and product season. Lactobacillus parabuchneri was found in the products from three of four factories. Various sourdough lactic acid bacteria (LAB) were identified in commercial TMR silage; Lactobacillus panis, Lactobacillus hammesii, Lactobacillus mindensis, Lactobacillus pontis, Lactobacillus frumenti and Lactobacillus farciminis were detected in many products. Moreover, changes owing to product season were distinctive, and Lact. pontis and Lact. frumenti became detectable in summer products. CONCLUSION:Sourdough LAB are involved in the ensiling of commercial TMR silage. Silage bacterial communities vary more by season than by factory. The LAB species Lact. parabuchneri was detected in the TMR silage but may not be essential to the product's long bunk life after silo opening. SIGNIFICANCE AND IMPACT OF THE STUDY:Commercial TMR silage resembles sourdough with respect to bacterial communities and long shelf life. The roles of sourdough LAB in the ensiling process and aerobic stability are worth examining.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Wang C,Nishino Ndoi
10.1111/j.1472-765X.2010.02915.xsubject
Has Abstractpub_date
2010-10-01 00:00:00pages
436-42issue
4eissn
0266-8254issn
1472-765Xpii
LAM2915journal_volume
51pub_type
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
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doi:10.1111/j.1472-765x.1994.tb00960.x
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
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更新日期:2004-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02295.x
更新日期:2008-02-01 00:00:00
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更新日期:2011-11-01 00:00:00
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journal_title:Letters in applied microbiology
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doi:10.1046/j.1472-765x.1997.00330.x
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更新日期:2002-01-01 00:00:00