Rapid RAPD analysis for distinguishing Listeria species and Listeria monocytogenes serotypes using a capillary air thermal cycler.

abstract：:Erwinia amylovora bacteria cause fire blight disease, which affects apple and pear production worldwide. The Erw. amylovora pyrC gene encodes a predicted dihydroorotase enzyme involved in pyrimidine biosynthesis. Here, we discovered that the Erw. amylovora pyrC244::Tn5 mutant was a uracil auxotroph. Unexpectedly, the ...

journal_title：Letters in applied microbiology

authors： Ramos LS,Sinn JP,Lehman BL,Pfeufer EE,Peter KA,McNellis TW

更新日期：2015-06-01 00:00:00

abstract：AIMS:To investigate the incidence of Campylobacter and Salmonella contamination associated with supermarket and butchers' shop chicken and related packaging. METHOD AND RESULTS:Three hundred raw samples (whole chicken, chicken breast with skin or chicken pieces) were purchased on a monthly basis for seven months. Pack...

journal_title：Letters in applied microbiology

authors： Harrison WA,Griffith CJ,Tennant D,Peters AC

更新日期：2001-12-01 00:00:00

abstract：AIMS:This study was aimed to investigate the effectiveness of high-pressure homogenization (HPH) against Alicyclobacillus acidoterrestris. METHODS AND RESULTS:The susceptibility of three different strains of A. acidoterrestris (DSMZ 2498, Gamma4 and c8) to HPH (500-1700 bar) was studied. The experiments were performed...

journal_title：Letters in applied microbiology

authors： Bevilacqua A,Cibelli F,Corbo MR,Sinigaglia M

更新日期：2007-10-01 00:00:00

abstract：:The metabolism of glucose was studied in Lactococcus lactis subsp. CNRZ 125 by 13C NMR. The initial rate of glucose utilization was higher for exponential phase cells than for stationary phase cells [150 vs 85 nmol g (dry wt)-1 s -1]. 31P NMR was used to determine changes in glycolytic phosphorylated intermediates (fr...

journal_title：Letters in applied microbiology

authors： Foucaud C,Herve M,Neumann JM,Hemme D

更新日期：1995-07-01 00:00:00

abstract：AIMS:To develop and evaluate a real-time PCR assay incorporating an internal amplification control (IAC) suitable for the screening of Shiga toxin (Stx)-producing Escherichia coli (STEC) in foods. METHODS AND RESULTS:A competitive IAC was constructed and included in an stx-specific real-time PCR assay. Coupled to 18-h...

journal_title：Letters in applied microbiology

authors： Auvray F,Lecureuil C,Dilasser F,Taché J,Derzelle S

更新日期：2009-05-01 00:00:00

abstract：:Listeria monocytogenes is a pathogenic bacterium, with human disease and infection linked to dairy products, seafood, ready-to-eat meat and raw & undercooked meats. Stainless steel is the most common food preparation surface and therefore, it is important to understand how food storage conditions such as surface mater...

journal_title：Letters in applied microbiology

authors： Redfern J,Verran J

更新日期：2017-04-01 00:00:00

abstract：:In order to select a suitable indicator for monitoring the pathogen regrowth potential of composted biosolids, the growth kinetics of selected bacteria were investigated. Growth parameters of six serovars of Salmonella and three strains of Escherichia coli in sterilized compost were compared. Seeded Salmonella and E. ...

journal_title：Letters in applied microbiology

authors： Sidhu J,Gibbs RA,Ho GE,Unkovich I

更新日期：1999-11-01 00:00:00

abstract：:Plant proteases play a fundamental role in several processes like growth, development and in response to biotic and abiotic stress. In particular, aspartic proteases (AP) are expressed in different plant organs and have antimicrobial activity. Previously, we purified an AP from Salpichroa origanifolia fruits called sa...

journal_title：Letters in applied microbiology

authors： Díaz ME,Rocha GF,Kise F,Rosso AM,Guevara MG,Parisi MG

更新日期：2018-08-01 00:00:00

abstract：AIMS:The objective of the present study was to determine the optimal culture conditions for exo-biopolymer production by Cordyceps militaris in shake flask culture. METHODS AND RESULTS:The optimal temperature and initial pH for both mycelial growth and exo-biopolymer production by Cordyceps militaris in shake flask cu...

journal_title：Letters in applied microbiology

authors： Park JP,Kim SW,Hwang HJ,Yun JW

更新日期：2001-07-01 00:00:00

abstract：UNLABELLED:Newly synthesized erythromycin derivatives were screened for synergy with oxacillin and other β-lactam antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). MRSA ATCC43300 and some clinically isolated MRSA were used. Several erythromycin derivatives were found to possess high synergism with...

journal_title：Letters in applied microbiology

authors： Li Z,He M,Dong X,Lin H,Ge H,Shen S,Li J,Ye RD,Chen D

更新日期：2015-04-01 00:00:00

abstract：:Leclercia adecarboxylata, a Gram-negative bacillus of family Enterobacteriaceae, is an uncommonly identified pathogen isolated from environmental and clinical specimens. Most of the human infections are polymicrobial and commonly occur in immunocompromised hosts, although nosocomial infections in immunocompetent hosts...

journal_title：Letters in applied microbiology

authors： Choudhary M,Choudhary BK,Bhoyar S,Kale SB,Chaudhari SP,Bera BC,Jain A,Barbuddhe SB

更新日期：2018-01-01 00:00:00

abstract：:Synthesis of gold nanoparticles (AuNPs) has become a needed domain of applied science. Biological method for synthesis of AuNPs by Trichoderma hamatum SU136 aqueous mycelial extract was achieved. The culture filtrate of the fungus was exposed to three different concentrations of gold chloride. The culture filtrate of ...

journal_title：Letters in applied microbiology

authors： Abdel-Kareem MM,Zohri AA

更新日期：2018-11-01 00:00:00

abstract：:Campylobacter jejuni was inoculated into a range of raw and ready-to-eat foods. These food samples were used as a test source for detection of this bacterium by the polymerase chain reaction (PCR). Specific detection of Camp. jejuni, as indicated by a PCR product of 159 bp, was possible with all pre-cooked deli-sliced...

journal_title：Letters in applied microbiology

authors： Winters DK,O'Leary AE,Slavik MF

更新日期：1998-09-01 00:00:00

abstract：AIMS:To evaluate the in vitro bactericidal activity of the novel antimicrobial peptide (AP) CECT7121 against Gram-positive bacteria from mastitic dairy cattle. METHODS AND RESULTS:A total of 15 Staphylococcus aureus, 10 Streptococcus dysgalactiae, 7 Strep. uberis, 1 Strep. agalactiae strains were isolated from 33 diff...

journal_title：Letters in applied microbiology

authors： Sparo MD,Jones DG,Sánchez Bruni SF

更新日期：2009-02-01 00:00:00

abstract：UNLABELLED:The mechanisms and organisms involved in the natural formation of volatile organohalogen compounds (VOX) are largely unknown. We provide evidence that the common and widespread soil bacterium Sinorhizobium meliloti strain 1021 is capable of producing up to 3338·6 ± 327·8 ng l(-1) headspace volume of chlorofo...

journal_title：Letters in applied microbiology

authors： Weigold P,Ruecker A,Jochmann M,Osorio Barajas XL,Lege S,Zwiener C,Kappler A,Behrens S

更新日期：2015-10-01 00:00:00

abstract：:Coliphages and enteroviruses were monitored over 12 months in sewage and air adjacent to an activated sludge plant. Both showed temporal variation but the mean count of phages in enterovirus-positive samples was not significantly different from that in enterovirus-negative samples. Hence coliphages are not necessarily...

journal_title：Letters in applied microbiology

authors： Carducci A,Arrighi S,Ruschi A

更新日期：1995-09-01 00:00:00

abstract：AIMS: The aim of this paper is to check the effect of salinity on the bioremediation process of petroleum hydrocarbons in the saline-alkaline soil. METHODS AND RESULTS: In this study, soil salinity was adjusted to different levels by water leaching method and the bioremediation process was conducted for 28 days. Soil ...

journal_title：Letters in applied microbiology

authors： Qin X,Tang JC,Li DS,Zhang QM

更新日期：2012-09-01 00:00:00

abstract：:Candida-associated denture stomatitis presents as erythema of the palatal mucosa and is caused by biofilms containing the fungus Candida albicans that co-reside with oral bacteria on the denture-fitting surface. This study aimed to assess the effect of several frequently encountered oral bacteria on the expression of ...

journal_title：Letters in applied microbiology

authors： Morse DJ,Wilson MJ,Wei X,Bradshaw DJ,Lewis MAO,Williams DW

更新日期：2019-04-01 00:00:00

abstract：:Iron availability plays an important role in the virulence of micro-organisms, which develops different systems for iron acquisition. The expression of genes involved in iron uptake systems is usually regulated by Fur, a transcriptional regulator. Plesiomonas shigelloides is a Gram-negative food- and water-borne enter...

journal_title：Letters in applied microbiology

authors： Rodríguez-Rodríguez S,Santos JA

更新日期：2018-04-01 00:00:00

abstract：:Salmonella enteritidis-inoculated poultry sausages were pressurized at 500 MPa by combining different times (10 and 30 min) and temperatures (50, 60 and 70 degrees C) or heat treated with the same temperature-time combinations and a standard cooking (75 degrees C for 30 min). Counts of Salm. enteritidis and mesophilic...

journal_title：Letters in applied microbiology

authors： Yuste J,Pla R,Mor-Mur M

更新日期：2000-11-01 00:00:00

abstract：AIMS:The objective of the study was to evaluate the variability of germination response of 10 strains of proteolytic Clostridium botulinum. METHODS AND RESULTS:An automated turbidometric method was used to follow the fall in optical density. Spores of proteolytic Cl. botulinum germinated in response to l-alanine alone...

journal_title：Letters in applied microbiology

authors： Alberto F,Broussolle V,Mason DR,Carlin F,Peck MW

更新日期：2003-01-01 00:00:00

abstract：UNLABELLED:In this study, we developed and validated a loop-mediated isothermal amplification (LAMP) assay for Salmonella detection targeting bcfD gene, a conserved fimbrial operon gene existing in Salmonella. The Salmonella LAMP assay we developed successfully amplified 44 Salmonella strains (14 standard strains and 3...

journal_title：Letters in applied microbiology

authors： Zhuang L,Gong J,Li Q,Zhu C,Yu Y,Dou X,Liu X,Xu B,Wang C

更新日期：2014-12-01 00:00:00

abstract：UNLABELLED:Antibiotic-resistant Salmonella strains were isolated from saladette and red round type tomatoes, and an analysis done of the antibacterial activity of roselle calyx extracts against any of the identified strains. One hundred saladette tomato samples and 100 red round tomato samples were collected from publi...

journal_title：Letters in applied microbiology

authors： Gutiérrez-Alcántara EJ,Rangel-Vargas E,Gómez-Aldapa CA,Falfan-Cortes RN,Rodríguez-Marín ML,Godínez-Oviedo A,Cortes-López H,Castro-Rosas J

更新日期：2016-02-01 00:00:00

abstract：AIMS:Acetic acid is considered an important preservative in tropical grass ensiling. The objective of the current experiments was to follow the ensiling fermentation of low dry matter (DM) tropical grass as a model to study changes in bacterial communities during acetic acid fermentation. METHODS AND RESULTS:Direct-cu...

journal_title：Letters in applied microbiology

authors： Nishino N,Li Y,Wang C,Parvin S

更新日期：2012-03-01 00:00:00

abstract：AIMS:To investigate the use of a Staphylococcus epidermidis transcriptional regulator gene as target for species-specific determination. METHODS AND RESULTS:Staph. epidermidis genes encoding putative transcriptional regulators were retrieved from GenBank and those showing no homology with other bacterial sequences wer...

journal_title：Letters in applied microbiology

authors： Liu D,Swiatlo E,Austin FW,Lawrence ML

更新日期：2006-09-01 00:00:00

abstract：AIMS:To isolate benzene-degrading strains from neutral and alkaline groundwaters contaminated by benzene, toluene, ethylbenzene, xylenes (BTEX) from the SIReN aquifer, UK, and to test their effective pH range and ability to degrade TEX. METHODS AND RESULTS:The 14 isolates studied had an optimum pH for growth of 8, and...

journal_title：Letters in applied microbiology

authors： Fahy A,Ball AS,Lethbridge G,Timmis KN,McGenity TJ

更新日期：2008-07-01 00:00:00

abstract：:The marine antimicrobial peptide NZ17074, a variant of arenicin-3 from Arenicola marina that has broad antimicrobial activity and high bioavailability, can be designed to treat bacterial and fungal diseases. To reduce the toxicity of NZ17074, N6 was designed by replacing a cysteine in positions 3 and 20 with alanine, ...

journal_title：Letters in applied microbiology

authors： Yang N,Wang X,Teng D,Mao R,Hao Y,Feng X,Wang J

更新日期：2017-09-01 00:00:00

abstract：:Blown pack spoilage (BPS) of vacuum packaged beef is caused by psychrotolerant and psychrophilic Clostridium species, primarily Clostridium estertheticum and Clostridium gasigenes. The aim of this study was to investigate the environmental niches and impact of season on these BPS Clostridium spp. on Irish beef farms. ...

journal_title：Letters in applied microbiology

authors： Esteves E,Whyte P,Gupta TB,Bolton DJ

更新日期：2020-12-01 00:00:00

abstract：:The antimicrobial activities of bovine lactoferrin (bLF), its pepsin hydrolysate (bLFH) and the active peptide lactoferricin B (LFcinB) against four clinical isolates of enterohaemorrhagic Escherichia coli O157:H7 were studied. The MICs against these isolates were 3 mg ml-1 for bLF, 0.1-0.2 mg ml-1 for bLFH and 8-10 m...

journal_title：Letters in applied microbiology

authors： Shin K,Yamauchi K,Teraguchi S,Hayasawa H,Tomita M,Otsuka Y,Yamazaki S

更新日期：1998-06-01 00:00:00

abstract：AIMS:Tuberculous pleurisy is an important cause of pleural effusions in areas with a high incidence of tuberculosis. In this study, we developed an IS1081-based LAMP for the detection of Mycobacterium tuberculosis complex and investigated its usefulness in the diagnosis of tuberculous pleurisy. METHODS AND RESULTS:Inv...

journal_title：Letters in applied microbiology

authors： Yang B,Wang X,Li H,Li G,Cao Z,Cheng X

更新日期：2011-11-01 00:00:00