Abstract:
UNLABELLED:Newly synthesized erythromycin derivatives were screened for synergy with oxacillin and other β-lactam antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). MRSA ATCC43300 and some clinically isolated MRSA were used. Several erythromycin derivatives were found to possess high synergism with oxacillin against MRSA. The newly synthesized erythromycin derivatives were also tested for their inhibitory effects against MRSA, either separately or in combination with oxacillin, using serial broth dilution, disc diffusion, Etest strips, growth curves and time-kill curves. A representative derivative, SIPI-8294, could potentiate almost all β-lactam antibiotics tested against the model strain MRSA ATCC43300 from 4 to 128 times and had synergism with oxacillin against 12 of 16 clinical isolates of MRSA under one-fourth of the minimum inhibitory concentration (MIC) of the compounds. This is the first report on the synergistic activity of these new erythromycin derivatives. These findings provide a new choice for the treatment of infection caused by MRSA and lead us to further study the synergistic mechanism. SIGNIFICANCE AND IMPACT OF THE STUDY:This study is the first report on the synergy of anti-MRSA between new erythromycin derivatives and β-lactam antibiotics in vitro. The results show that although the erythromycin derivatives have poor anti-MRSA effects alone, they possess high synergism with oxacillin against MRSA ATCC43300 and clinically isolated MRSA. These novel compounds can significantly reduce the dosage of β-lactam antibiotics against MRSA, while this synergistic effect is different from the combination of β-lactams and β-lactamase inhibitors. The research may provide a new choice for the treatment of infection caused by MRSA and be useful to the research and development of new combination of medicines.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Li Z,He M,Dong X,Lin H,Ge H,Shen S,Li J,Ye RD,Chen Ddoi
10.1111/lam.12378subject
Has Abstractpub_date
2015-04-01 00:00:00pages
352-8issue
4eissn
0266-8254issn
1472-765Xjournal_volume
60pub_type
杂志文章abstract::Yellow wine lees (YWL) are the main co-products in yellow wine industry with unbalanced amino acid (AA) profiles. Solid-state fermentation was employed in this study to upgrade the YWL for ruminant animals. A 3 × 3 orthogonal design was conducted to optimize the fermentation condition for optimal crude protein (CP) yi...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13078
更新日期:2018-12-01 00:00:00
abstract:AIMS:To determine the production of reuterin by lactobacilli isolates from pig faeces and to evaluate their potential as probiotic bacteria. METHODS AND RESULTS:Twenty-eight of 165 lactobacilli isolates produced reuterin in the presence of glycerol. Six isolates yielding high levels of reuterin with respect to type st...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01390.x
更新日期:2003-01-01 00:00:00
abstract::Growth at low positive temperatures and induced phenotypic resistance to extreme cold temperature (freezing/thawing cycles) of Enterococcus faecalis were investigated. The effect of low temperatures on the specific growth rates was studied; use of Arrhenius profile and Ratkovsky 'square-root' model allowed determinati...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1996.tb01345.x
更新日期:1996-12-01 00:00:00
abstract:AIMS:To isolate, characterize and identify lactic acid bacteria (LAB) in dochi (fermented black beans), a traditional fermented food in Taiwan. METHODS AND RESULTS:A total of 30 samples were collected from three different dochi producers and analysed after different periods of storage. Fifty-two cultures of LAB were i...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01922.x
更新日期:2006-08-01 00:00:00
abstract:AIMS:To develop a specific, fast and simple molecular method useful to detect the entomopathogenic bacterium Pseudomonas entomophila. METHODS AND RESULTS:The use of bioinformatics tools allowed the identification of unique genes present in P. entomophila genome. Using such genes, we designed primers aiming to detect s...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02785.x
更新日期:2010-03-01 00:00:00
abstract::Typical haemolytic uraemic syndrome (STEC-HUS), caused by Shiga toxin (Stx)-producing Escherichia coli (STEC), is a serious, life-threating disease that mainly affects children. Bacteriological and genetic tests are commonly used in the routine laboratory diagnosis of STEC-HUS; however, serological methods have emerge...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13295
更新日期:2020-06-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2012.03230.x
更新日期:2012-05-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12823
更新日期:2018-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03141.x
更新日期:2011-11-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12390
更新日期:2015-05-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1994.tb00458.x
更新日期:1994-11-01 00:00:00
abstract::Adhesion of three Lactobacillus strains onto human epithelial intestinal Caco-2 and Int-407 cell lines was compared. More adhesion occurred onto Int-407. The trypsin and sodium periodate pretreatment of bacteria revealed different mechanisms of adhesion depending on the Caco-2 and Int-407, involving carbohydrates and ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1996.tb01198.x
更新日期:1996-06-01 00:00:00
abstract:AIMS:To establish a rapid and reliable multiplex PCR (mPCR)-based method allowing specific identification of Carnobacterium piscicola SF668 during storage of cold smoked salmon (CSS). METHODS AND RESULTS:CSS was inoculated with C. piscicola SF668 and stored at 4 degrees C. Samples were withdrawn at regular time interv...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2005.01696.x
更新日期:2005-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12554
更新日期:2016-04-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1998.00386.x
更新日期:1998-08-01 00:00:00
abstract::The purpose of this investigation was to evaluate intracanal bacterial reduction by cryotreatment using a dental instrument equipped with a duct and connected to a cryogenic fluid source. A total of 86 roots were infected with Enterococcus faecalis and incubated. After incubation, the contaminated roots were divided i...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12017
更新日期:2013-02-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.00842.x
更新日期:2000-12-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12227
更新日期:2014-06-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01434.x
更新日期:2004-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03183.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13061
更新日期:2018-11-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01948.x
更新日期:2006-09-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2008.02406.x
更新日期:2008-09-01 00:00:00
abstract:AIMS:To investigate the use of mannitol salt agar (MSA) supplemented with acriflavine for selective growth and quantification of Staphylococcus aureus from flushed dairy manure wastewater (FDMW). METHODS AND RESULTS:Minimal inhibitory concentrations of acriflavine in MSA were determined by comparing the growth of S. a...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01915.x
更新日期:2006-06-01 00:00:00
abstract:UNLABELLED:Lactobacillus acidophilus LAB20 has potential to be a probiotic strain because it can be present at high numbers in the jejunum of dog. To specifically detect LAB20 from dog faecal samples, a real-time PCR protocol was developed targeting the novel surface (S) layer protein gene of LAB20. The presence of S-l...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12117
更新日期:2013-10-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13201
更新日期:2019-10-01 00:00:00
abstract:AIMS:To develop an assay for rapid screening of bacterial adhesion to various groups of biomolecules present in fish mucus. METHODS AND RESULTS:A novel assay was developed for investigation of bacterial adhesion to various groups of mucus biomolecules from fish. Lipid-, protein-, carbohydrate- and nucleic acid-rich co...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12003
更新日期:2013-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01338.x
更新日期:2003-01-01 00:00:00
abstract::Cow's milk was inoculated with ca 10(3) and 10(7) cfu ml-1 Escherichia coli O157:H7. After fermentation at 42 degrees C for 0-5 h, the yoghurt was stored at 4 degrees C. Two kinds of yoghurt were used: traditional yoghurt (TY), made with Streptococcus thermophilus and Lactobacillus bulgaricus starter cultures, and 'bi...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1997.00067.x
更新日期:1997-05-01 00:00:00
abstract::The hydrogen peroxide (H2O2) stress response in Enterococcus faecalis ATCC19433 was investigated. A 2.4 mmol l-1 H2O2 pretreatment conferred protection against a lethal concentration (45 mmol l-1) of this agent. The relatively high concentrations of H2O2 used for adaptation and challenge treatments in Ent. faecalis em...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1998.00325.x
更新日期:1998-04-01 00:00:00