Antibacterial activity of bovine lactoferrin and its peptides against enterohaemorrhagic Escherichia coli O157:H7.

Abstract:

:The antimicrobial activities of bovine lactoferrin (bLF), its pepsin hydrolysate (bLFH) and the active peptide lactoferricin B (LFcinB) against four clinical isolates of enterohaemorrhagic Escherichia coli O157:H7 were studied. The MICs against these isolates were 3 mg ml-1 for bLF, 0.1-0.2 mg ml-1 for bLFH and 8-10 micrograms ml-1 for LFcinB in 1% Bactopeptone broth. LFcinB killed these bacteria within 3 h at concentrations above 10 micrograms ml-1. Transmission electron microscopy findings suggested that LFcinB acts on the bacterial surface and affects cytoplasmic contents. LFcinB was shown to influence the levels of verotoxins in the culture supernatant fluid of an E. coli O157:H7 strain. These results demonstrate that E. coli O157:H7 strains are susceptible to the antimicrobial effects of bLF and its peptides.

journal_name

Lett Appl Microbiol

authors

Shin K,Yamauchi K,Teraguchi S,Hayasawa H,Tomita M,Otsuka Y,Yamazaki S

doi

10.1046/j.1472-765x.1998.00358.x

subject

Has Abstract

pub_date

1998-06-01 00:00:00

pages

407-11

issue

6

eissn

0266-8254

issn

1472-765X

journal_volume

26

pub_type

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