Abstract:
AIMS:To provide comparative genome sequence data for two related model strains of Staphylococcus aureus (SH1000 and 8325-4) that are used extensively in laboratory research. METHODS AND RESULTS:Comparative genome sequencing was used to identify genetic differences between Staph. aureus SH1000 and the fully genome-sequenced ancestral strain, Staph. aureus NCTC 8325. PCR amplification and DNA sequencing were employed to determine which of the genetic polymorphisms identified were also present in Staph. aureus 8325-4, a direct derivative of 8325 and the parent strain of SH1000. Aside from known genetic differences between these strains, Staph. aureus SH1000 harboured 15 single-nucleotide polymorphisms compared with 8325 (of which 12 were also found in 8325-4), and a 63-bp deletion upstream of the spa gene not present in either 8325 or 8325-4. CONCLUSIONS:Staphylococcus aureus SH1000 and 8325-4 contain a number of genetic polymorphisms relative to the progenitor strain of the lineage (8325) and to each other. SIGNIFICANCE AND IMPACT OF THE STUDY:The comparative genome sequences of SH1000 and 8325-4 presented here define the genotypes of two key strains in staphylococcal laboratory research and reveal genetic polymorphisms that may impact their phenotypic properties.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
O'Neill AJdoi
10.1111/j.1472-765X.2010.02885.xsubject
Has Abstractpub_date
2010-09-01 00:00:00pages
358-61issue
3eissn
0266-8254issn
1472-765Xpii
LAM2885journal_volume
51pub_type
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journal_title:Letters in applied microbiology
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doi:10.1046/j.1472-765x.2000.00700.x
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journal_title:Letters in applied microbiology
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