Comparison of three immunological methods for detecting staphylococcal enterotoxins from food.

abstract：AIMS:In this study, a capillary polymerase chain reaction (cPCR) was applied for Salmonella detection from poultry meat. METHODS AND RESULTS:Salmonella detection limits of the optimized cPCR were determined with DNA templates from the samples of tetrathionate broth (TTB), Rappaport Vassiliadis broth (RVB) and selenite...

journal_title：Letters in applied microbiology

authors： Gunaydin E,Eyigor A,Carli KT

更新日期：2007-01-01 00:00:00

abstract：:Iron availability plays an important role in the virulence of micro-organisms, which develops different systems for iron acquisition. The expression of genes involved in iron uptake systems is usually regulated by Fur, a transcriptional regulator. Plesiomonas shigelloides is a Gram-negative food- and water-borne enter...

journal_title：Letters in applied microbiology

authors： Rodríguez-Rodríguez S,Santos JA

更新日期：2018-04-01 00:00:00

abstract：:Fresh-cut produce is at greater risk of Salmonella contamination. Detection and early warning systems play an important role in reducing the dissemination of contaminated products. One-step Reverse Transcription Polymerase Chain Reaction (RT-qPCR) targeting Salmonella tmRNA with or without a 6-h enrichment was evaluat...

journal_title：Letters in applied microbiology

authors： Miao YJ,Xiong GT,Bai MY,Ge Y,Wu ZF

更新日期：2018-05-01 00:00:00

abstract：AIMS:To isolate, characterize and identify lactic acid bacteria (LAB) in dochi (fermented black beans), a traditional fermented food in Taiwan. METHODS AND RESULTS:A total of 30 samples were collected from three different dochi producers and analysed after different periods of storage. Fifty-two cultures of LAB were i...

journal_title：Letters in applied microbiology

authors： Chen YS,Yanagida F,Hsu JS

更新日期：2006-08-01 00:00:00

abstract：:The contribution of trehalose and hsp104 to barotolerance in Saccharomyces cerevisiae has been investigated. Mutant strains, which lacked the ability to accumulate trehalose and/or hsp104, were examined for barotolerance and thermotolerance. All the mutants showed lower barotolerance and thermotolerance than their con...

journal_title：Letters in applied microbiology

authors： Iwahashi H,Obuchi K,Fujii S,Komatsu Y

更新日期：1997-07-01 00:00:00

abstract：:Colicin V is a small, proteinaceous bacterial toxin, produced by many strains of Escherichia coli and other members of the Enterobacteriaceae, that fits the definition of class II bacteriocins of Gram-positive bacteria. Export of colicin V is dependent on specific ABC (ATP-binding cassette) secretion proteins which re...

journal_title：Letters in applied microbiology

authors： McCormick JK,Klaenhammer TR,Stiles ME

更新日期：1999-07-01 00:00:00

abstract：:The present study aimed to investigate the incidence, virulence and antibiotic properties in Vibrio spp. isolated from cockles (Tegillarca granosa) marketed in Korea. A total of 32 Vibrio spp. isolates including V. parahaemolyticus (n = 4), V. alginolyticus (n = 11), V. diabolicus (n = 14) and V. harveyi (n = 3) were ...

journal_title：Letters in applied microbiology

authors： Dahanayake PS,Hossain S,Wickramanayake MVKS,Heo GJ

更新日期：2020-07-01 00:00:00

abstract：:For pyruvate-producing strains, intracellular reduced nicotinamide adenine dinucleotide (NADH) accumulation is the main reason for the glycolysis inhibition. Comparing with glucose, using sodium gluconate as carbon source brought a decrease in NADH production and an increase in pyruvate production in engineered strain...

journal_title：Letters in applied microbiology

authors： Yang M,Mu T,Zhong W,Olajuyin AM,Xing J

更新日期：2017-08-01 00:00:00

abstract：AIMS:The aim of this study was to detect the main pathogenic human RNA enteric viruses able to persist in the environment such as astrovirus, enterovirus, norovirus and hepatitis A virus (HAV) in shellfish collected from two locations in northern Tunisia. METHODS AND RESULTS:Viruses were eluted from digestive tissues ...

journal_title：Letters in applied microbiology

authors： Elamri DE,Aouni M,Parnaudeau S,Le Guyader FS

更新日期：2006-10-01 00:00:00

abstract：UNLABELLED:Aspergillus fumigatus, a well-known human and animal pathogen causing aspergillosis, has been historically identified by morphological and microscopic features. However, recent studies have shown that species identification on the basis of morphology alone is problematic. The aim of this work was to confirm ...

journal_title：Letters in applied microbiology

authors： Soleiro CA,Pena GA,Cavaglieri LR,Coelho I,Keller LM,Dalcero AM,Rosa CA

更新日期：2013-12-01 00:00:00

abstract：:A sandwich enzyme-linked immunosorbent assay to detect staphylococcal enterotoxin B was developed using rat monoclonal antibodies as capture antibodies and as a biotinylated conjugate. This test was sensitive, less than 1 ng/ml of enterotoxin B was detected and interference by protein A was prevented by the use of rat...

journal_title：Letters in applied microbiology

authors： Rogemond V,Da Costa Castro JM,Delaunay T,Cazaux C,Guinet RM

更新日期：1991-09-01 00:00:00

abstract：:A total of 87 isolates of Yersinia enterocolitica were examined with randomly amplified polymorphic DNA (RAPD) by use of three different primers. Based on the RAPD profiles, the strains could be divided into three major groups: (1) the pathogenic American serotypes, O:8, O:13ab, O:20 and O:21; (2) the pathogenic Europ...

journal_title：Letters in applied microbiology

authors： Rasmussen HN,Olsen JE,Rasmussen OF

更新日期：1994-11-01 00:00:00

abstract：:In this study, a total of 172 putative omega-3 producers were isolated from 28 sediment samples from the Arabian Gulf employing a selective isolation procedure using marine agar containing 0·1% triphenyl tetrazolium chloride (TTC). Out of these 172 isolates, 19 isolates produced eicosapentaenoic acid (EPA) as confirme...

journal_title：Letters in applied microbiology

authors： Alagarsamy S,Sabeena Farvin KH,Fakhraldeen S,Kooramattom MR,Al-Yamani F

更新日期：2019-08-01 00:00:00

abstract：UNLABELLED:Sandalwood oil has been found in numerous therapeutic applications in traditional medicines such as Chinese traditional medicine and Ayurveda. However, there are no comparative accounts available in the literature that focused on in vitro and in vivo tree sample-derived extracts. Combined dichloromethane and...

journal_title：Letters in applied microbiology

authors： Misra BB,Dey S

更新日期：2012-12-01 00:00:00

abstract：:In order to select a suitable indicator for monitoring the pathogen regrowth potential of composted biosolids, the growth kinetics of selected bacteria were investigated. Growth parameters of six serovars of Salmonella and three strains of Escherichia coli in sterilized compost were compared. Seeded Salmonella and E. ...

journal_title：Letters in applied microbiology

authors： Sidhu J,Gibbs RA,Ho GE,Unkovich I

更新日期：1999-11-01 00:00:00

abstract：:Live shellfish samples (120) were collected from nine littoral sites in Brittany (western France). They were screened for Listeria spp. and a count of faecal coliforms was carried out. Analysis of the results revealed Listeria spp. in 55% of samples, a much higher rate than the previous, infrequent, recorded data. Fur...

journal_title：Letters in applied microbiology

authors： Monfort P,Minet J,Rocourt J,Piclet G,Cormier M

更新日期：1998-03-01 00:00:00

abstract：UNLABELLED:This study reports the development of a loop-mediated isothermal amplification (LAMP) reaction for the detection of Pythium myriotylum. The primer set targeting the ITS sequence of P. myriotylum worked most efficiently at 60°C and allowed the detection of P. myriotylum DNA within 30 min by fluorescence monit...

journal_title：Letters in applied microbiology

authors： Fukuta S,Takahashi R,Kuroyanagi S,Ishiguro Y,Miyake N,Nagai H,Suzuki H,Tsuji T,Hashizume F,Watanabe H,Kageyama K

更新日期：2014-07-01 00:00:00

abstract：:White Spot Syndrome Virus (WSSV) is a dsDNA virus causing White Spot Syndrome Disease (WSSD) in shrimp with almost 100% morality rate within 3-10 days. In Bangladesh, WSSD is one of the major impediments of shrimp farming. This study first investigated the prevalence and distribution of WSSV in cultured shrimps of the...

journal_title：Letters in applied microbiology

authors： Hossain A,Nandi SP,Siddique MA,Sanyal SK,Sultana M,Hossain MA

更新日期：2015-02-01 00:00:00

abstract：:The detection of foodborne viruses in bivalve molluscs is a challenging procedure in relation to low virus concentration and to the presence of significant RT-PCR inhibitors. The aim of this study was the development of an efficient direct extraction method for foodborne viral RNA from bivalve molluscs. Using Mengovir...

journal_title：Letters in applied microbiology

authors： Quang Le H,Suffredini E,Tien Pham D,Kim To A,De Medici D

更新日期：2018-11-01 00:00:00

abstract：AIMS:Adaptation of a colorimetric assay using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) to evaluate the cytotoxicity of the extracellular products of micro-organisms pathogenic to fish. METHODS AND RESULTS:The optimal conditions for the colorimetric assay were determined and this method was...

journal_title：Letters in applied microbiology

authors： Zorrilla I,Balebona MC,Moriñigo MA

更新日期：2001-11-01 00:00:00

abstract：AIMS:To apply scanning electron microscopy, image analysis and a fluorescent viability stain to assess lethal and sublethal injury in food-borne bacteria exposed to pulsed-plasma gas discharges (PPGD). METHODS AND RESULTS:The fluorescent redox probe 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) was used for enumerati...

journal_title：Letters in applied microbiology

authors： Rowan NJ,Espie S,Harrower J,Farrell H,Marsili L,Anderson JG,MacGregor SJ

更新日期：2008-01-01 00:00:00

abstract：:A set of Escherichia coli freshwater isolates was chosen to compare the effectiveness of denaturing gradient gel electrophoresis (DGGE) vs temporal temperature gradient gel electrophoresis (TTGE) for separating homologous amplicons from the respective uidA region differing in one to seven single base substitutions. Bo...

journal_title：Letters in applied microbiology

authors： Farnleitner AH,Kreuzinger N,Kavka GG,Grillenberger S,Rath J,Mach RL

更新日期：2000-06-01 00:00:00

abstract：:The Crystal Enteric/Nonfermenter (E/NF) identification kit (Becton Dickinson Microbiology Systems, USA) was evaluated using water-derived bacterial isolates and results compared to those obtained by the API 20E system (BioMérieux, UK). Both the E/NF and 20E systems correctly identified 93% of the Enterobacteriaceae re...

journal_title：Letters in applied microbiology

authors： Micklewright IJ,Sartory DP

更新日期：1995-09-01 00:00:00

abstract：AIMS:To develop a novel method for rapid construction of Campylobacter jejuni deletion mutants. METHODS AND RESULTS:We used overlapping extension PCR protocol to amplify a target sequence region of Camp. jejuni genomic DNA in which an internal fragment, Cj0618 coding sequence, was replaced by a chloramphenicol resista...

journal_title：Letters in applied microbiology

authors： Hansen CR,Khatiwara A,Ziprin R,Kwon YM

更新日期：2007-12-01 00:00:00

abstract：:Using a novel capillary thermal cycler, randomly amplified polymorphic DNA (RAPD) generated DNA fingerprints were obtained in 3 h. The RAPD profiles were produced using a random 10-mer primer (5'-ACCGCCTGCT-3') which discriminated between different Listeria spp. Unique fragment profiles of Listeria monocytogenes serot...

journal_title：Letters in applied microbiology

authors： Black SF,Gray DI,Fenlon DR,Kroll RG

更新日期：1995-03-01 00:00:00

abstract：AIMS:The goal of the study was to develop a reliable, reproducible and rapid method of culture in order to screen a large number of fungal transformants. METHODS AND RESULTS:The method is based upon miniaturized cell cultures and automated expression screening in microwell plates. For the method development, 50 recomb...

journal_title：Letters in applied microbiology

authors： Alberto F,Navarro D,de Vries RP,Asther M,Record E

更新日期：2009-08-01 00:00:00

abstract：:Understanding mechanisms of the novel SARS-CoV2 infection and progression can provide potential novel targets for prevention and/or treatment. This could be achieved via the inhibition of viral entry and/or replication, or by suppression of the immunologic response that is provoked by the infection (known as the cytok...

journal_title：Letters in applied microbiology

authors： Akour A

更新日期：2020-09-01 00:00:00

abstract：AIMS:This paper attempts to develop a kinetic model to describe the growth of aerobic granules developed under different operation conditions. METHODS AND RESULTS:A series of experiments were conducted by using four-column sequencing batch reactors to study the formation of aerobic granules under different conditions,...

journal_title：Letters in applied microbiology

authors： Yang SF,Liu QS,Tay JH,Liu Y

更新日期：2004-01-01 00:00:00

abstract：AIMS:Enzyme kinetics of purified laccases from six different Pleurotus ostreatus strains were determined in the oxidation of syringaldazine, guaiacol and ABTS. METHODS AND RESULTS:Significant differences in the kinetic constants were found. Catalytic activity (kcat) ranged from 19 to 941 U mg(-1) for syringaldazine, f...

journal_title：Letters in applied microbiology

authors： Tinoco R,Pickard MA,Vazquez-Duhalt R

更新日期：2001-05-01 00:00:00

abstract：:A lead-resistant bacteria isolated from soil contaminated with car battery waste were identified as Providencia alcalifaciens based on biochemical characteristics, FAME profile and 16S rRNA sequencing and designated as strain 2EA. It resists lead nitrate up to 0·0014 mol l(-1) by precipitating soluble lead as insolubl...

journal_title：Letters in applied microbiology

authors： Naik MM,Khanolkar D,Dubey SK

更新日期：2013-02-01 00:00:00