Abstract:
AIMS:In this study, a capillary polymerase chain reaction (cPCR) was applied for Salmonella detection from poultry meat. METHODS AND RESULTS:Salmonella detection limits of the optimized cPCR were determined with DNA templates from the samples of tetrathionate broth (TTB), Rappaport Vassiliadis broth (RVB) and selenite cystine broth (SCB) artificially contaminated with 10-fold dilutions of 6 x 10(8) CFU ml(-1) of pure Salmonella enterica ssp. enterica serovar Enteritidis 64K stock culture. Detection limits of cPCR from TTB, RVB and SCB were found as 6, 6 x 10(1) and 6 x 10(4) CFU ml(-1), respectively. In addition, detection limits of bacteriology were also determined as 6 CFU ml(-1) with TTB and SCB, and 6 x 10(1) CFU ml(-1) with RVB. A total of 200 samples, consisting of 100 chicken and 100 turkey meat samples, were tested with optimized cPCR and bacteriology. Eight and six per cent of the chicken meat samples were found to harbour Salmonella by cPCR and standard bacteriology, respectively. Of six Salmonella isolates, four belonged to serogroup D, two to serogroup B. CONCLUSIONS:The TTB cultures of both artificially and naturally contaminated samples were found to be superior to those of RVB and SCB cultures in their cPCR results. This cPCR, utilizing template from 18-h TTB primary enrichment broth culture, takes approximately 40 min in the successful detection of Salmonella from poultry meat. SIGNIFICANCE AND IMPACT OF THE STUDY:This study shows that cPCR from TTB enrichment culture of poultry meat would enable rapid detection of Salmonella in laboratories with low sample throughput and limited budget.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Gunaydin E,Eyigor A,Carli KTdoi
10.1111/j.1472-765X.2006.02033.xsubject
Has Abstractpub_date
2007-01-01 00:00:00pages
24-9issue
1eissn
0266-8254issn
1472-765Xpii
LAM2033journal_volume
44pub_type
杂志文章abstract:AIMS:Evaluation of the activity profiles of standard prokaryotic translation inhibitors with different physicochemical properties under whole-cell and cell-free conditions. METHODS AND RESULTS:The minimal inhibitory concentration values (cell-free/whole-cell microg ml(-1)) for three aminoglycosides (neomycin, 0.01/6.9...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02281.x
更新日期:2008-02-01 00:00:00
abstract::Environmental air sampling was evaluated as a method to detect the presence of M. bovis in the vicinity of infected badgers and their setts. Airborne particles were collected on gelatine filters using a commercially available air sampling instrument and tested for the presence of M. bovis using bacteriological culture...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12055
更新日期:2013-05-01 00:00:00
abstract:AIMS:This paper attempts to develop a kinetic model to describe the growth of aerobic granules developed under different operation conditions. METHODS AND RESULTS:A series of experiments were conducted by using four-column sequencing batch reactors to study the formation of aerobic granules under different conditions,...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.2003.01452.x
更新日期:2004-01-01 00:00:00
abstract:UNLABELLED:The unicellular freshwater green microalga Haematococcus pluvialis is the richest source of natural astaxanthin. Since accumulation of astaxanthin differs significantly among various algal strains at different stages, it is therefore critical to develop an effective high-throughput assay for rapid screening ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12531
更新日期:2016-02-01 00:00:00
abstract:AIMS: To develop a new instrument of great versatility for recovering micro-organisms from all types of food samples and to compare the effects with existing sample preparation methods. METHODS AND RESULTS: To detach micro-organisms from large-size unbroken food samples such as apples, carrots, potatoes and tomatoes w...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2012.03289.x
更新日期:2012-09-01 00:00:00
abstract:AIMS:Overproduced alpha-amylases in Bacillus subtilis provoke a specific stress response involving the CssRS two-component system, which controls expression of the HtrA and HtrB proteases. Previously, the B. subtilis TepA protein was implicated in high-level alpha-amylase secretion. Our present studies were aimed at in...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01539.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:Several cases of campylobacteriosis reported worldwide seemingly conflict with the strict growth requirements and sensitivity to environmental stress of Campylobacter jejuni. In this study, the need for a micro-aerobic environment [dissolved oxygen tension (DOT): 0.1-90%; 100% air saturation)] and the adaptive res...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2008.02326.x
更新日期:2008-03-01 00:00:00
abstract::Candida albicans is an opportunistic fungus, which causes vulvovaginal candidiasis (VVC). The aim of this study was to evaluate Mrr2 mutation and its expression levels and Candida drug resistance 1 (Cdr1) in C. albicans associated with fluconazole (FCA) resistance. We identified 80 isolates of C. albicans from 155 vag...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13248
更新日期:2020-02-01 00:00:00
abstract::Five phage types have been identified in 38 strains of Salmonella enteritidis isolated in Turkey in the 20-month period June 1992-January 1994. Strains belonging to phage type 4 predominated. Within phage type 4, plasmid profile typing has proved a useful method of strain discrimination and has confirmed the identity ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1994.tb00960.x
更新日期:1994-10-01 00:00:00
abstract::Coffee (Coffea arabica L.) is currently grown in many tropical and subtropical areas countries and is a major traded commodity for the developing world. Coffee leaf blight, caused by Phomopsis heveicola, is one of the most important fungal diseases dangerous to coffee crops in China. This study aimed to develop a PCR-...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13396
更新日期:2020-09-26 00:00:00
abstract:AIMS:The study was carried out to evaluate the use of randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) as a method for the identification of lactobacilli isolated from meat products. METHODS AND RESULTS:RAPD-PCR with primers M13 and D8635 was applied to the identification and intraspecific diffe...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.00939.x
更新日期:2001-07-01 00:00:00
abstract:AIMS:To investigate the spatial organization of endogenous and exogenously applied Lactobacillus communities at specific locations in the adult gastrointestinal tract of different hosts. METHODS AND RESULTS:Samples of the human, murine and avian gastrointestinal tract of subjects that received or not received a Lactob...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02994.x
更新日期:2011-03-01 00:00:00
abstract:AIMS:To test the inhibitory activity of 2-nitro-1-propanol (2NPOH) against Salmonella Typhimurium, Escherichia coli O157:H7 and Enterococcus faecalis. METHODS AND RESULTS:Specific growth rates (h(-1)) of S. Typhimurium, E. coli O157:H7 and Ent. faecalis were determined during culture in tryptic soya broth (TSB) supple...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01613.x
更新日期:2004-01-01 00:00:00
abstract::Enterococci were isolated from faecal droppings of chickens in broiler and layer farms and the susceptibilities to nine therapeutic antimicrobial agents and six growth-promoting antibiotics were determined by the agar dilution method. Resistance to therapeutic antimicrobial agents such as ampicillin, clindamycin, eryt...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.00842.x
更新日期:2000-12-01 00:00:00
abstract::A lead-resistant bacteria isolated from soil contaminated with car battery waste were identified as Providencia alcalifaciens based on biochemical characteristics, FAME profile and 16S rRNA sequencing and designated as strain 2EA. It resists lead nitrate up to 0·0014 mol l(-1) by precipitating soluble lead as insolubl...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12026
更新日期:2013-02-01 00:00:00
abstract::From a genomic library of Mycoplasma hyopneumoniae a 1.3 kb DNA fragment was cloned which showed specific Southern hybridization and dot hybridization with the type strain of several porcine and bovine Mycoplasma species. This probe selectively recognized M. hyopneumoniae sequences in purified DNA or in broth-grown or...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1991.tb00551.x
更新日期:1991-06-01 00:00:00
abstract::The pattern of DNA fragments produced by the polymerase chain reaction using arbitrary primers is strongly influenced by the age of the colonies sampled. We show that the number, clarity and reproducibility of the bands produced is optimal when very young colonies are sampled. ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1993.tb01467.x
更新日期:1993-12-01 00:00:00
abstract::Candida species are increasingly important fungal pathogens. The reaction of rat alveolar macrophages (AM) to Candida albicans was compared with that to C. glabrata and C. krusei. Phagocytosis of C. glabrata was similar to that of C. albicans, but significantly slower for C. krusei due to reduced attachment. After ops...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1997.00200.x
更新日期:1997-09-01 00:00:00
abstract:AIMS:Real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on a TaqMan-minor groove binder (MGB) probe was developed for the rapid detection of avian influenza virus subtype H5. METHODS AND RESULTS:Conserved regions in the haemagglutinin genes of avian influenza viruses subtype H5 served as ta...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02253.x
更新日期:2008-01-01 00:00:00
abstract:AIMS:2-Keto-4-methylthiobutyric acid (KMBA) is an established intermediate in microbial biosynthesis of ethylene from methionine. This study demonstrates the kinetics and effects of trace elements and electron complexes on substrate (KMBA)-derived C2H4 biosynthesis in soil. METHODS AND RESULTS:We have previously repor...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01590.x
更新日期:2004-01-01 00:00:00
abstract::The recent rise in the awareness of the occurrence of toxic cyanobacterial blooms in aquatic environments, with associated human health problems and animal deaths, has increased the need for rapid, reliable and sensitive methods of determining cyanobacterial toxicity. A luminescent bacterial toxicity test was assessed...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1990.tb00161.x
更新日期:1990-10-01 00:00:00
abstract::Fifty-five isolates of nine species and two species varieties (5 isolates each) of the Aspergillus niger group were tested for progesterone transformation. Generally, all isolates of the different species transformed progesterone to 21-hydroxyprogesterone. In addition, the isolates of seven species have the capacity t...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1995.tb00438.x
更新日期:1995-04-01 00:00:00
abstract::Colistin-resistant Pseudomonas aeruginosa (CRPA), as a health care system threat, is increasing globally. This study aimed was to determine CRPA by high-resolution melting curve (HRM) analysis method. The HRM method was done on standard strains of P. aeruginosa and CRPA strains. Ninefold serial dilutions of known geno...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13270
更新日期:2020-04-01 00:00:00
abstract:AIMS:The aim of this study was to determine the antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20 against several micro-organisms, including Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi. METHODS AND RESULTS:Antimicrobial and ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02818.x
更新日期:2010-04-01 00:00:00
abstract::Bulk tank milk from 160 producers was tested for Listeria monocytogenes at three monthly intervals over 1 year. Twenty-five producers were positive, most on a single occasion, only seven were positive on three or more of the four samplings. Listeria monocytogenes numbers were low, usually < 1 ml-1, the highest was 35 ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1995.tb00407.x
更新日期:1995-01-01 00:00:00
abstract:AIMS:The current study examined the antimicrobial susceptibility of 86 Listeria spp. isolated from processed bison carcasses. MATERIALS AND METHODS:Susceptibility to 25 antimicrobial agents was determined using E-test and National Antimicrobial Resistance Monitoring System (NARMS) panels. Most Listeria isolates (88-98...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.02027.x
更新日期:2007-01-01 00:00:00
abstract::Coliphages and enteroviruses were monitored over 12 months in sewage and air adjacent to an activated sludge plant. Both showed temporal variation but the mean count of phages in enterovirus-positive samples was not significantly different from that in enterovirus-negative samples. Hence coliphages are not necessarily...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1995.tb01042.x
更新日期:1995-09-01 00:00:00
abstract:AIMS:The study aim was to determine the presence of total and faecal coliforms on kitchen surfaces, in tap water and on the hands of caregivers in households on both sides of the US-Mexico border. METHODS AND RESULTS:Samples were collected in 135 randomly selected households in Ciudad Juarez, Mexico, and El Paso, Texa...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2008.02378.x
更新日期:2008-06-01 00:00:00
abstract:AIMS:From a survey of submerged culture of edible mushrooms, a high pigment-producing fungus Paecilomyces sinclairii was selected and its optimal culture conditions investigated. METHODS AND RESULTS:The optimal culture conditions for pigment production were as follows: inoculum age, 3 d; temperature, 25 degrees C; ini...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01168.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:This study evaluated the inactivation of virulent Yersinia pestis dried on polymers, steel, and glass surfaces using vapour-phase hydrogen peroxide. METHODS AND RESULTS:A suspension of Y. pestis CO92 (1.70 x 10(8) CFU) was dried on 10 different types of test surfaces and exposed to vapour-phase hydrogen peroxide ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.2008.02421.x
更新日期:2008-10-01 00:00:00