Development and evaluation of a new device to effectively detach micro-organisms from food samples.

abstract：AIMS:To demonstrate that produce rinsates used for RT-qPCR detection of foodborne viruses may cause significant PCR inhibition and propose a means to reduce its impact on sensitivity. METHODS AND RESULTS:Here, it is shown that rinsing and concentration from spinach and precut lettuce have the potential to generate RNA...

journal_title：Letters in applied microbiology

authors： Plante D,Bélanger G,Leblanc D,Ward P,Houde A,Trottier YL

更新日期：2011-03-01 00:00:00

abstract：:The present study was aimed at determining antimicrobial susceptibility by a CLSI standard microdilution testing protocol and detecting the resistance genes of motile Aeromonas species isolated from cultured fish. The importance of the minimum inhibitory concentrations was assessed based on statistically determined ep...

journal_title：Letters in applied microbiology

authors： Duman M,Saticioglu IB,Altun S

更新日期：2020-11-01 00:00:00

abstract：AIMS:To evaluate the accuracy of pyrosequencing for the description of Phytophthora communities in terms of taxa identification and risk of assignment for false Molecular Operational Taxonomic Units (MOTUs). METHODS AND RESULTS:Pyrosequencing of Internal Transcribed Spacer 1 (ITS1) amplicons was used to describe the s...

journal_title：Letters in applied microbiology

authors： Vettraino AM,Bonants P,Tomassini A,Bruni N,Vannini A

更新日期：2012-11-01 00:00:00

abstract：AIMS:  To show that application of copper hydroxide citrus sprays mixed with field source water (possibly contaminated) will not support Escherichia coli on plant surfaces. Environmental stresses of transient phyllosphere bacteria and presence of copper will eradicate these bacteria before harvest. METHODS AND RESULTS...

journal_title：Letters in applied microbiology

authors： Narciso JA,Ference CM,Ritenour MA,Widmer WW

更新日期：2012-02-01 00:00:00

abstract：AIMS:To assess the effectiveness of sequential treatments of radish seeds with aqueous chlorine dioxide (ClO(2) ) and dry heat in reducing the number of Escherichia coli O157:H7. METHODS AND RESULTS:Radish seeds containing E. coli O157:H7 at 5.5 log CFU g(-1) were treated with 500 μg ml(-1) ClO(2) for 5 min and subseq...

journal_title：Letters in applied microbiology

authors： Bang J,Kim H,Kim H,Beuchat LR,Kim Y,Ryu JH

更新日期：2011-10-01 00:00:00

abstract：AIMS:The study aim was to determine the presence of total and faecal coliforms on kitchen surfaces, in tap water and on the hands of caregivers in households on both sides of the US-Mexico border. METHODS AND RESULTS:Samples were collected in 135 randomly selected households in Ciudad Juarez, Mexico, and El Paso, Texa...

journal_title：Letters in applied microbiology

authors： Carrasco L,Mena KD,Mota LC,Ortiz M,Behravesh CB,Gibbs SG,Bristol JR,Mayberry L,Cardenas VM

更新日期：2008-06-01 00:00:00

abstract：AIMS:To isolate, characterize and identify lactic acid bacteria (LAB) in dochi (fermented black beans), a traditional fermented food in Taiwan. METHODS AND RESULTS:A total of 30 samples were collected from three different dochi producers and analysed after different periods of storage. Fifty-two cultures of LAB were i...

journal_title：Letters in applied microbiology

authors： Chen YS,Yanagida F,Hsu JS

更新日期：2006-08-01 00:00:00

abstract：:The antimicrobial activities of bovine lactoferrin (bLF), its pepsin hydrolysate (bLFH) and the active peptide lactoferricin B (LFcinB) against four clinical isolates of enterohaemorrhagic Escherichia coli O157:H7 were studied. The MICs against these isolates were 3 mg ml-1 for bLF, 0.1-0.2 mg ml-1 for bLFH and 8-10 m...

journal_title：Letters in applied microbiology

authors： Shin K,Yamauchi K,Teraguchi S,Hayasawa H,Tomita M,Otsuka Y,Yamazaki S

更新日期：1998-06-01 00:00:00

abstract：AIMS:To develop a PCR-based method for quantitative detection of Fusarium asiaticum (Fa) and Fusarium graminearum (Fg) in wheat seeds. METHODS AND RESULTS:Based on the sequences of the cyp51A gene, two primer pairs FaF + FaR and FgF + FgR were developed for the species-specific detection of Fa and Fg, respectively. To...

journal_title：Letters in applied microbiology

authors： Yin Y,Liu X,Ma Z

更新日期：2009-06-01 00:00:00

abstract：:A new ultrasonic apparatus operating at a frequency of 40 kHz was developed to dislodge biofilms from food processing equipment in order to assess the effectiveness of cleaning protocols. Sonication conditions to remove biofilms and quantification by ATP-bioluminescence are described. An industrial meat process was de...

journal_title：Letters in applied microbiology

authors： Oulahal-Lagsir N,Martial-Gros A,Boistier E,Blum LJ,Bonneau M

更新日期：2000-01-01 00:00:00

abstract：:Nineteen strains of vancomycin-resistant Enterococcus faecium isolated from 10 of 75 (13.3%) tenderloin beef samples were examined for resistance to selected antibiotics, presence of plasmids, and genetic diversity by random amplification of polymorphic DNA analysis. All strains showed multiple resistant to the antibi...

journal_title：Letters in applied microbiology

authors： Son R,Nimita F,Rusul G,Nasreldin E,Samuel L,Nishibuchi M

更新日期：1999-08-01 00:00:00

abstract：AIMS:The aim of this study is to develop an RT-PCR assay combined with immunomagnetic beads (IMS/RT-PCR) coating monoclonal antibody (Mab) for separation and detection of norovirus (genogroup II) in faecal samples. We furthermore compare its detection limits with IMS/RT-PCR using polyclonal antibody (Pab) and the TRIzo...

journal_title：Letters in applied microbiology

authors： Yao L,Wu Q,Wang D,Kou X,Zhang J

更新日期：2009-08-01 00:00:00

abstract：:The marine antimicrobial peptide NZ17074, a variant of arenicin-3 from Arenicola marina that has broad antimicrobial activity and high bioavailability, can be designed to treat bacterial and fungal diseases. To reduce the toxicity of NZ17074, N6 was designed by replacing a cysteine in positions 3 and 20 with alanine, ...

journal_title：Letters in applied microbiology

authors： Yang N,Wang X,Teng D,Mao R,Hao Y,Feng X,Wang J

更新日期：2017-09-01 00:00:00

abstract：:Shigella spp. are water-borne pathogens responsible for mild to severe cases bacilli dysentery all around the world known as Shigellosis. The progressively increasing of antibiotic resistance among Shigella calls for developing and establishing novel alternative therapeutic methods. The present study aimed to evaluate...

journal_title：Letters in applied microbiology

authors： Shahin K,Zhang L,Bao H,Hedayatkhah A,Soleimani-Delfan A,Komijani M,He T,Barazandeh M,Mansoorianfar M,Bouzari M,Wang R

更新日期：2020-10-18 00:00:00

abstract：UNLABELLED:The influence of oenological factors on cold-active pectinases from 15 preselected indigenous yeasts belonging to Aureobasidium pullulans, Filobasidium capsuligenum, Rhodotorula dairenensis, Cryptococcus saitoi and Saccharomyces cerevisiae was investigated. Pectinolytic enzymes were constitutive or partially...

journal_title：Letters in applied microbiology

authors： Merín MG,Morata de Ambrosini VI

更新日期：2015-05-01 00:00:00

abstract：AIM:To compare the incidence of antimicrobial resistance among Salmonella serotypes isolated in a pig slaughterhouse in Zaragoza (Spain) during 1993 and 2001. METHODS AND RESULTS:A total of 168 isolates representing 10 serotypes were examined by disc diffusion method using 17 antibiotics. Data showed that the majority...

journal_title：Letters in applied microbiology

authors： Agustín AI,Carramiñana JJ,Rota C,Herrera A

更新日期：2005-01-01 00:00:00

abstract：AIMS:The aim of this study was to investigate the role of divercin V41 in inhibition and prevention of Listeria monocytogenes. METHODS AND RESULTS:Carnobacterium divergens V41 deficient in bacteriocin production was isolated and characterized by enzyme-liked immunosorbent assay, multiplex polymerase chain reaction and...

journal_title：Letters in applied microbiology

authors： Richard C,Brillet A,Pilet MF,Prévost H,Drider D

更新日期：2003-01-01 00:00:00

abstract：AIMS:The objective of this work was to purify the tyrosinase from Bacillusthuringiensis subsp. kurstaki (Bt) (CCTCC AB 90010) and study its enzymatic properties. METHODS AND RESULTS:A 'one-step' purification method was used in this work, which was an easy, high-yield purification method. Tyrosinase activity of this pu...

journal_title：Letters in applied microbiology

authors： Liu N,Zhang T,Wang YJ,Huang YP,Ou JH,Shen P

更新日期：2004-01-01 00:00:00

abstract：:The objective of this research was to develop novel cellulose acetate (biopolymer) composite materials with an excellent antimicrobial activity by embedding molybdenum trioxide particles with unique high specific surface area. High surface area molybdenum trioxide particles were prepared from freshly precipitated moly...

journal_title：Letters in applied microbiology

authors： Shafaei S,Dörrstein J,Guggenbichler JP,Zollfrank C

更新日期：2017-01-01 00:00:00

abstract：UNLABELLED:Faecal indicator bacteria (FIB) and harmful algal blooms (HABs) threaten the health and the economy of coastal communities worldwide. Emerging automated sampling technologies combined with molecular analytical techniques could enable rapid detection of micro-organisms in-situ, thereby improving resource mana...

journal_title：Letters in applied microbiology

authors： Yamahara KM,Demir-Hilton E,Preston CM,Marin R 3rd,Pargett D,Roman B,Jensen S,Birch JM,Boehm AB,Scholin CA

更新日期：2015-08-01 00:00:00

abstract：AIMS:To compare the effects of five yeast probiotic formulations on viability, revival and washout kinetic in the digestive tract of mice, and the protection against an experimental infection with Salmonella enterica serovar Typhimurium. METHODS AND RESULTS:The number of viable cells in five commercial probiotic produ...

journal_title：Letters in applied microbiology

authors： Martins FS,Veloso LC,Arantes RM,Nicoli JR

更新日期：2009-12-01 00:00:00

abstract：AIMS:To compare a range of enrichment broths and enrichment temperatures for the isolation of Escherichia coli O157 by immunomagnetic separation (IMS) from sandy, loam and clay soils. METHODS AND RESULTS:Soils were spiked with cocktails of four atoxigenic strains of E. coli O157 and four strains of commensal E. coli. ...

journal_title：Letters in applied microbiology

authors： Hepburn NF,MacRae M,Johnston M,Mooney J,Ogden ID

更新日期：2002-01-01 00:00:00

abstract：:The small cryptic plasmid pMB1 (1.9 kb), previously isolated from Bifidobacterium longum, has been characterized by physical mapping. Two cloning vectors, pMR3 and pDG7, carrying chloramphenicol and ampicillin resistances derived from pJH101, have been electroporated in Escherichia coli. ...

journal_title：Letters in applied microbiology

authors： Matteuzzi D,Brigidi P,Rossi M,Di D

更新日期：1990-10-01 00:00:00

abstract：AIMS:To isolate and identify diazotrophic endophytes in the stem of Japanese sweetpotato cv. Koganesengan. METHODS AND RESULTS:Surface-sterilized and thinly sliced (1-2 mm) sweetpotato stem samples were incubated in test tubes with semi-solid modified Rennie (MR) medium. The test tubes were assayed for acetylene reduc...

journal_title：Letters in applied microbiology

authors： Asis CA Jr,Adachi K

更新日期：2004-01-01 00:00:00

abstract：AIMS:Breast milk has been described as a source of bacteria influencing the development of the infant gut microbiota. Up to the present, few studies have been focused on the application of culture-independent techniques to study bacterial diversity in breast milk. In this context, the aim of this study was to character...

journal_title：Letters in applied microbiology

authors： Collado MC,Delgado S,Maldonado A,Rodríguez JM

更新日期：2009-05-01 00:00:00

abstract：UNLABELLED:Although little evidence existed to support that view, European countries and in particular France, have regarded echinoderms, including sea urchins, as low risk in terms of feacal contamination. It is hypothesized that the sea urchins mode of feeding, which is based on grazing and differs from bivalve mollu...

journal_title：Letters in applied microbiology

authors： Bouchoucha M,Piquet JC,Chavanon F,Dufresne C,Le Guyader FS

更新日期：2016-02-01 00:00:00

abstract：AIM:To study the effect of glucose concentrations on the growth by Brettanomyces bruxellensis yeast strain in batch experiments and develop a mathematical model for kinetic behaviour analysis of yeast growing in batch culture. METHODS AND RESULTS:A Matlab algorithm was developed for the estimation of model parameters....

journal_title：Letters in applied microbiology

authors： Aguilar-Uscanga MG,Garcia-Alvarado Y,Gomez-Rodriguez J,Phister T,Delia ML,Strehaiano P

更新日期：2011-08-01 00:00:00

abstract：:Polyhydroxyalkanoates (PHAs) are polyesters accumulated by many bacteria under unbalanced growth conditions, and have been used to meet the various demands in areas of agriculture, medicine, and materials especially belong to a rapidly expanding area of biomedical research. Unfortunately, the high production cost than...

journal_title：Letters in applied microbiology

authors： Liu CC,Zhang LL,An J,Chen B,Yang H

更新日期：2016-01-01 00:00:00

abstract：AIMS:In this study, the relationship between morphology and cephalosporin C (CPC) production in a 30-l bioreactor culture of Cephalosporium acremonium M25 using a 3:7 seed mixture was investigated. In addition, the kinetic model was established and applied. METHODS AND RESULTS:CPC production was performed in a 30-l bi...

journal_title：Letters in applied microbiology

authors： Kim JH,Lim JS,Kim CH,Kim SW

更新日期：2005-01-01 00:00:00

abstract：:The recent rise in the awareness of the occurrence of toxic cyanobacterial blooms in aquatic environments, with associated human health problems and animal deaths, has increased the need for rapid, reliable and sensitive methods of determining cyanobacterial toxicity. A luminescent bacterial toxicity test was assessed...

journal_title：Letters in applied microbiology

authors： Lawton LA,Campbell DL,Beattie KA,Codd GA

更新日期：1990-10-01 00:00:00