Abstract：
AIMS:To demonstrate that produce rinsates used for RT-qPCR detection of foodborne viruses may cause significant PCR inhibition and propose a means to reduce its impact on sensitivity. METHODS AND RESULTS:Here, it is shown that rinsing and concentration from spinach and precut lettuce have the potential to generate RNA extracts that are inhibitory to RT-qPCRs assembled from commercial kits for the detection of norovirus GII (NoV GII), hepatitis A virus (HAV), hepatitis E virus (HEV), rotavirus (RV) and feline calicivirus (FCV) as sample process control. It is further shown that the addition of bovine serum albumin (BSA) to those reactions restored a positive signal in all cases. The effect of BSA was dependent upon the primer/probe combination. Moreover, two of the detection systems (FCV and HAV) strongly benefited from the addition of BSA even in the absence of PCR inhibitors. CONCLUSIONS:BSA was shown to restore positive signals in five different RT-qPCR systems that were otherwise completely inhibited by produce rinsate extracts. It is therefore suggested to consider the addition of BSA to RT-qPCRs for the detection of foodborne viruses when inhibition is observed. SIGNIFICANCE AND IMPACT OF THE STUDY:This study clearly demonstrates the potency of PCR inhibitors generated during routine virus concentration from produce and that it can be alleviated by the addition of BSA to the RT-qPCRs. Although used elsewhere, the addition of BSA to PCRs is not a common practice in this growing field of research.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Plante D,Bélanger G,Leblanc D,Ward P,Houde A,Trottier YLdoi
10.1111/j.1472-765X.2010.02989.xsubject
Has Abstractpub_date
2011-03-01 00:00:00pages
239-44issue
3eissn
0266-8254issn
1472-765Xjournal_volume
52pub_type
杂志文章abstract：AIMS:This study assessed the plant growth-promoting ability of the bacterial strains Ochrobactrum intermedium (isolate CrT-1) and Bacillus cereus (isolate S-6). METHODS AND RESULTS:Two chromium resistant bacterial strains isolated from chromium-contaminated wastewater and soils were identified as O. intermedium CrT-1 ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2006.01977.x
更新日期：2006-10-01 00:00:00
abstract：AIMS:Acetic acid is considered an important preservative in tropical grass ensiling. The objective of the current experiments was to follow the ensiling fermentation of low dry matter (DM) tropical grass as a model to study changes in bacterial communities during acetic acid fermentation. METHODS AND RESULTS:Direct-cu...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2011.03191.x
更新日期：2012-03-01 00:00:00
abstract：AIMS: To show that application of copper hydroxide citrus sprays mixed with field source water (possibly contaminated) will not support Escherichia coli on plant surfaces. Environmental stresses of transient phyllosphere bacteria and presence of copper will eradicate these bacteria before harvest. METHODS AND RESULTS...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2011.03179.x
更新日期：2012-02-01 00:00:00
abstract：AIMS:To investigate the growth of salmonellae on sprouting alfalfa seeds as affected by the inoculum size, microbial load and Pseudomonas fluorescens 2-79. METHODS AND RESULTS:Alfalfa seeds pre-inoculated with < or =10(1)-10(3) CFU g(-1) of salmonellae and with or without Ps. fluorescens 2-79 were sprouted in glass ja...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2007.02302.x
更新日期：2008-02-01 00:00:00
abstract：AIMS:To evaluate the accuracy of pyrosequencing for the description of Phytophthora communities in terms of taxa identification and risk of assignment for false Molecular Operational Taxonomic Units (MOTUs). METHODS AND RESULTS:Pyrosequencing of Internal Transcribed Spacer 1 (ITS1) amplicons was used to describe the s...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.2012.03310.x
更新日期：2012-11-01 00:00:00
abstract：AIMS:To determine the effect of cold storage on the survival of Erwinia amylovora. METHODS AND RESULTS:The survival of E. amylovora was assessed during storage at 2 degrees C. Populations of E. amylovora inoculated into phosphate-buffered saline remained static, whereas in nutrient media populations increased at low t...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2003.01407.x
更新日期：2003-01-01 00:00:00
abstract：:A new ultrasonic apparatus operating at a frequency of 40 kHz was developed to dislodge biofilms from food processing equipment in order to assess the effectiveness of cleaning protocols. Sonication conditions to remove biofilms and quantification by ATP-bioluminescence are described. An industrial meat process was de...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2000.00653.x
更新日期：2000-01-01 00:00:00
abstract：:A native isolate Lactobacillus farciminis MD isolated from fermenting mushroom exhibited a high degree of sensitivity to the majority of the bacteriocins produced by strains of lactobacilli, leuconostoc and pediococci. Also, the efficacy of Lact. farciminis MD as a sensitive strain for antibiotic assay was established...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2000.00691.x
更新日期：2000-03-01 00:00:00
abstract：:Currently used alum precipitated and oil adjuvant vaccines against HS caused by Pasteurella multocida B:2, have side effects and short-lived immunity, leading to regular catastrophic outbreaks in bovines in Asian subcontinent. The need for the development of an improved vaccine with longer immunity and the ability to ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.13010
更新日期：2018-09-01 00:00:00
abstract：:To enhance the overall expression level of lipase isozymes which catalyse the same reaction in Pichia pastoris through co-expression of isozymes from different sources; several types of co-expression ways were constructed to determine the co-expression efficiencies of lipase isozymes in P. pastoris. The results showed...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12783
更新日期：2017-10-01 00:00:00
abstract：:A sandwich enzyme-linked immunosorbent assay to detect staphylococcal enterotoxin B was developed using rat monoclonal antibodies as capture antibodies and as a biotinylated conjugate. This test was sensitive, less than 1 ng/ml of enterotoxin B was detected and interference by protein A was prevented by the use of rat...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.1991.tb00601.x
更新日期：1991-09-01 00:00:00
abstract：AIMS:To determine which citric acid-producing strain of Aspergillus niger utilized wet corn distillers grains most effectively to produce citric acid. METHODS AND RESULTS:Citric acid and biomass production by the fungal strains were analysed on the untreated grains or autoclaved grains using an enzyme assay and a grav...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2006.01958.x
更新日期：2006-09-01 00:00:00
abstract：AIMS:To investigate interactions between rumen protozoa and Shiga toxin-producing Escherichia coli (STEC) and to ascertain whether it is likely that rumen protozoa act as ruminant hosts for STEC. METHODS AND RESULTS:The presence of stx genes in different microbial fractions recovered from cattle and sheep rumen conten...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2004.01642.x
更新日期：2005-01-01 00:00:00
abstract：:Candida species are increasingly important fungal pathogens. The reaction of rat alveolar macrophages (AM) to Candida albicans was compared with that to C. glabrata and C. krusei. Phagocytosis of C. glabrata was similar to that of C. albicans, but significantly slower for C. krusei due to reduced attachment. After ops...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.1997.00200.x
更新日期：1997-09-01 00:00:00
abstract：AIMS:In this study, a capillary polymerase chain reaction (cPCR) was applied for Salmonella detection from poultry meat. METHODS AND RESULTS:Salmonella detection limits of the optimized cPCR were determined with DNA templates from the samples of tetrathionate broth (TTB), Rappaport Vassiliadis broth (RVB) and selenite...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2006.02033.x
更新日期：2007-01-01 00:00:00
abstract：AIMS:In this study, we compared different methods of colistin susceptibility testing, disc diffusion, agar dilution and Etest using a set of Enterobacteriaceae isolates that included colistin-resistant strains. METHODS AND RESULTS:Susceptibility of 200 clinical isolates of Enterobacteriaceae to colistin was tested to ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2011.03145.x
更新日期：2011-11-01 00:00:00
abstract：AIMS:To determine the production of reuterin by lactobacilli isolates from pig faeces and to evaluate their potential as probiotic bacteria. METHODS AND RESULTS:Twenty-eight of 165 lactobacilli isolates produced reuterin in the presence of glycerol. Six isolates yielding high levels of reuterin with respect to type st...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2003.01390.x
更新日期：2003-01-01 00:00:00
abstract：AIMS:Oenococcus oeni is a lactic acid bacterium used in wine fermentation. Two open reading frames (orfB and orfC) were identified in the upstream region of the hsp18 gene, encoding the small heat-shock protein Lo18. Expression of these genes in conditions of acid stress was studied in Escherichia coli. METHODS AND RE...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2001.00960.x
更新日期：2001-08-01 00:00:00
abstract：AIMS:From a survey of submerged culture of edible mushrooms, a high pigment-producing fungus Paecilomyces sinclairii was selected and its optimal culture conditions investigated. METHODS AND RESULTS:The optimal culture conditions for pigment production were as follows: inoculum age, 3 d; temperature, 25 degrees C; ini...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2002.01168.x
更新日期：2002-01-01 00:00:00
abstract：:A strain named as Pseudomonas aeruginosa 2016NX1, which could produce phenazine and cereusitin, was isolated from the root of Millettia specisoa. Phenazines were extracted, isolated and purified by chloroform, thin-layer chromatography, column chromatography and high-performance liquid chromatography. Then the purifie...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.13332
更新日期：2020-09-01 00:00:00
abstract：AIMS:To identify and characterize amino acid transport in Leuconostoc mesenteroides. METHODS AND RESULTS:The transport of labelled amino acids was measured in whole cells of Leuc. mesenteroides CNRZ 1273. Systems were operative under physiological conditions of growth, energy dependent and differed from peptide transp...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2002.01191.x
更新日期：2002-01-01 00:00:00
abstract：:The growth of the oxidatively imperfect yeast Candida maltosa Komagata, Nakase et Katsuya was studied experimentally and modelled mathematically in relation to sodium benzoate and lactic acid concentrations at different temperatures. Application of gamma models for the growth rate resulted in determination of cardinal...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12803
更新日期：2017-11-01 00:00:00
abstract：AIMS:The objective was to demonstrate the size of silver particles produced by the filamentous fungus Phoma sp.3.2883 via adsorption and accumulation, and to confirm that this silver was in a reduced state. METHODS AND RESULTS:Mycelium was freeze-dried and then shake-cultured in a silver nitrate solution. It was found...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2003.01348.x
更新日期：2003-01-01 00:00:00
abstract：:Leuconostoc strains were characterized according to their antibiotic susceptibilities, carbohydrate fermentation profiles, sucrase activity patterns and plasmid content. All the strains tested were resistant to the antibiotics sulphathiazole, trimethoprim and vancomycin, and could be separated into two groups based on...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2001.00891.x
更新日期：2001-03-01 00:00:00
abstract：:The genus Taylorella is composed of two species: (i) Taylorella equigenitalis, the causative agent of CEM, a venereally transmitted infection of Equidae and (ii) Taylorella asinigenitalis, a closely related species considered to be nonpathogenic, although experimental infection of mares with this bacterium resulted in...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12297
更新日期：2014-10-01 00:00:00
abstract：:Irreversible electroporation (IRE) damages cell membranes and is used in medicine for nonthermal ablation of malignant tumours. Our aim was to evaluate the antimicrobial effect of IRE. The pathogenic micro-organisms, Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa and Candida al...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12996
更新日期：2018-07-01 00:00:00
abstract：AIMS:To examine factors affecting faecal shedding of the foodborne pathogens Escherichia coli O157:H7 and Salmonella in dairy cattle and evaluate antimicrobial susceptibility of these isolates. METHODS:Faecal samples were obtained in replicate from lactating (LAC; n = 60) and non-lactating (NLAC; n = 60) Holstein catt...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2003.01417.x
更新日期：2003-01-01 00:00:00
abstract：AIMS:To screen for the existence and determine the structure of Tn916-like element in Streptococcus parauberis serotype II strains isolated from cultured Japanese flounder in western Japan. METHODS AND RESULTS:In this study, the structure of Tn916-like element and the flanking regions were characterized by polymerase ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2009.02609.x
更新日期：2009-06-01 00:00:00
abstract：AIMS:To survey for hepatitis A virus (HAV) and hepatitis E virus (HEV) contamination in edible bivalve shellfish. METHODS AND RESULTS:A total of 213 shellfish (52 oysters, 69 cockles and 92 mussels) collected from a culture farm and two retailed markets were investigated for HAV and HEV contamination by reverse transc...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2011.03152.x
更新日期：2011-12-01 00:00:00
abstract：AIMS:To isolate, characterize and identify lactic acid bacteria (LAB) in dochi (fermented black beans), a traditional fermented food in Taiwan. METHODS AND RESULTS:A total of 30 samples were collected from three different dochi producers and analysed after different periods of storage. Fifty-two cultures of LAB were i...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2006.01922.x
更新日期：2006-08-01 00:00:00
