Abstract:
AIMS:To survey for hepatitis A virus (HAV) and hepatitis E virus (HEV) contamination in edible bivalve shellfish. METHODS AND RESULTS:A total of 213 shellfish (52 oysters, 69 cockles and 92 mussels) collected from a culture farm and two retailed markets were investigated for HAV and HEV contamination by reverse transcription-polymerase chain reaction (RT-PCR) assay using HA2-HA1 (capsid region) and HE366-HE363 (ORF2/3 overlapping region) primers, respectively. It was found that 3.8% of the shellfish and 2.9 and 6.5% of the cockle and mussel, respectively, showed positive for HAV detection. Nucleotide sequencing of all the 8 HAV-positive shellfish revealed 97-100% similarity to HAV subgenotype IA. Interestingly, viruses were found more frequently in the gills than in digestive tissue (4.5%vs 0.5%, P = 0.045). All the shellfish were negative for HEV. CONCLUSION:Significant contamination of HAV in edible bivalve shellfish was observed. Beside digestive tissue, gills are one of the important samples for viral genome detection. SIGNIFICANCE AND IMPACT OF THE STUDY:HAV-contaminated shellfish can play a role as reservoirs and/or vehicles in faecal-oral transmission in Thailand, and further monitoring of such a contamination is required.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Namsai A,Louisirirotchanakul S,Wongchinda N,Siripanyaphinyo U,Virulhakul P,Puthavathana P,Myint KS,Gannarong M,Ittapong Rdoi
10.1111/j.1472-765X.2011.03152.xsubject
Has Abstractpub_date
2011-12-01 00:00:00pages
608-13issue
6eissn
0266-8254issn
1472-765Xjournal_volume
53pub_type
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