Abstract：
AIMS:The aim of this study was to elucidate if the need for iron for Escherichia coli to remain cultivable in a poorly nutritive medium such as the drinking water uses the iron transport system via the siderophores. METHODS AND RESULTS:Environmental strains of E. coli (isolated from a drinking water network), referenced strains of E. coli and mutants deficient in TonB, an essential protein for iron(III) acquisition, were incubated for 3 weeks at 25 degrees C, in sterile drinking water with and without lepidocrocite (gamma-FeOOH), an insoluble iron corrosion product. Only cells with a functional iron transport system were able to survive throughout the weeks. CONCLUSIONS:The iron transport system via protein TonB plays an essential role on the survival of E. coli in a weakly nutritive medium like drinking water. SIGNIFICANCE AND IMPACTS OF THE STUDY:Iron is a key parameter involved in coliform persistence in drinking water distribution systems.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Grandjean D,Jorand F,Guilloteau H,Block JCdoi
10.1111/j.1472-765X.2006.01895.xsubject
Has Abstractpub_date
2006-07-01 00:00:00pages
111-7issue
1eissn
0266-8254issn
1472-765Xpii
LAM1895journal_volume
43pub_type
杂志文章abstract：:The objective of this study was to undertake a microbiological survey of foods, animal faeces and wastewater samples for Clostridium difficile, and determine the genotypes and antimicrobial susceptibilities of isolates. A total of 211 samples were tested for C. difficile using culture methods. Thirteen toxigenic C. di...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.13238
更新日期：2020-01-01 00:00:00
abstract：:The physical effects of high-intensity pulsed electric fields (PEF) on the inactivation of diarrhoeagenic Bacillus cereus cells suspended in 0.1% peptone water were examined by transmission electron microscopy (TEM). The levels of PEF-induced microbial cell death were determined by enumeration on tryptone soy yeast ex...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2000.00772.x
更新日期：2000-08-01 00:00:00
abstract：:A native isolate Lactobacillus farciminis MD isolated from fermenting mushroom exhibited a high degree of sensitivity to the majority of the bacteriocins produced by strains of lactobacilli, leuconostoc and pediococci. Also, the efficacy of Lact. farciminis MD as a sensitive strain for antibiotic assay was established...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2000.00691.x
更新日期：2000-03-01 00:00:00
abstract：AIMS:The risk of hepatitis C virus infection in hospital environments can be assessed not only by studying epidemiological data and work practices, but also by the detection of these viruses (or indicators thereof) in health-care settings, on instruments etc. METHODS:Since standardized techniques specific to this end ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2002.01066.x
更新日期：2002-01-01 00:00:00
abstract：AIMS:To screen a pair of primers suitable for denaturing gradient gel electrophoretic (DGGE) analysis of ruminal methanogenic Archaea and to detect the archaeal communities in the rumen of goat. METHODS AND RESULTS:Nine primer pairs for 16S rDNA of methanogenic Archaea, including six for directed polymerase chain reac...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2009.02583.x
更新日期：2009-05-01 00:00:00
abstract：AIMS:This work was carried out to determine if there was a difference in the microbial population of the rumen associated with daylength at which sheep are housed. METHODS AND RESULTS:Denaturing gradient gel electrophoresis (DGGE) was used to study the ciliate and bacterial diversity in the rumen of Soay rams kept in ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2005.01707.x
更新日期：2005-01-01 00:00:00
abstract：AIMS:Aim of the study was to develop a medium for optimal heparinase production with a strain of Aspergillus flavus (MTCC-8654) by using a multidimensional statistical approach. METHODS AND RESULTS:Statistical optimization of intracellular heparinase production by A. flavus, a new isolate, was investigated. Plackett-B...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2009.02640.x
更新日期：2009-08-01 00:00:00
abstract：AIMS:To test the suitability of cheese whey powder (CWP) solution for ethanol fermentation and to compare performances of different Kluyveromyces marxianus strains for ethanol fermentation from CWP solution. METHODS AND RESULTS:Batch ethanol fermentation of cheese whey (CW), CWP and lactose solutions with the same ini...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2007.02132.x
更新日期：2007-06-01 00:00:00
abstract：UNLABELLED:In this study, we developed and validated a loop-mediated isothermal amplification (LAMP) assay for Salmonella detection targeting bcfD gene, a conserved fimbrial operon gene existing in Salmonella. The Salmonella LAMP assay we developed successfully amplified 44 Salmonella strains (14 standard strains and 3...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12328
更新日期：2014-12-01 00:00:00
abstract：AIMS:Breast milk has been described as a source of bacteria influencing the development of the infant gut microbiota. Up to the present, few studies have been focused on the application of culture-independent techniques to study bacterial diversity in breast milk. In this context, the aim of this study was to character...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2009.02567.x
更新日期：2009-05-01 00:00:00
abstract：AIMS: To develop a specific and highly sensitive loop-mediated isothermal amplification (LAMP) technique for the rapid detection of canine parvovirus (CPV) DNA directly in suspected faecal samples of dogs by employing a simple method of template preparation. METHODS AND RESULTS: LAMP reaction was developed by designin...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2012.03284.x
更新日期：2012-09-01 00:00:00
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journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2006.01884.x
更新日期：2006-05-01 00:00:00
abstract：AIMS:To compare the bacteriostatic and bactericidal activity of 13 chemotyped essential oils (EO) on 65 bacteria with varying sensitivity to antibiotics. METHODS AND RESULTS:Fifty-five bacterial strains were tested with two methods used for evaluation of antimicrobial activity (CLSI recommendations): the agar dilution...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2008.02406.x
更新日期：2008-09-01 00:00:00
abstract：AIMS:This work aims to investigate the binding capability of certain domains at N terminus of the protein NP_785232 of Lactobacillus plantarum to Caco-2 cells and to test the usage of affinity chromatography to isolate the human mucus component that interacts with them. METHODS AND RESULTS:Recombinant proteins contain...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2010.02911.x
更新日期：2010-10-01 00:00:00
abstract：:A method for developing a single-transposon-insertion mutant from a double-insertion mutant in Rhizobium is described. An exopolysaccharide (EPS)-defective mutant containing two Tn5-lacZ insertions was complemented with cloned wild-type DNA for EPS synthesis. One of the Tn5-lacZ insertions from the mutant was transfer...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.1994.tb00927.x
更新日期：1994-09-01 00:00:00
abstract：AIMS:To examine the efficacy of liposome oral administration to induce systemic and mucosal immune responses against verotoxin-producing Escherichia coli (VTEC) and the effect of the induced antibodies on the binding of the bacteria to Caco-2 cells. METHODS AND RESULTS:Mice were immunized orally with VTEC antigen and ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2003.01278.x
更新日期：2003-01-01 00:00:00
abstract：AIM:To evaluate the efficacy of electrochemically activated solution (ECASOL) in decontaminating Bacillus anthracis Ames and Vollum 1B spores, with and without changing the source water hardness and final ECASOL pH. METHODS AND RESULTS:Five different ECASOL formulations were generated, in which the source water hardne...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2006.02002.x
更新日期：2006-11-01 00:00:00
abstract：:Understanding mechanisms of the novel SARS-CoV2 infection and progression can provide potential novel targets for prevention and/or treatment. This could be achieved via the inhibition of viral entry and/or replication, or by suppression of the immunologic response that is provoked by the infection (known as the cytok...
journal_title：Letters in applied microbiology
pub_type： 杂志文章,评审
doi：10.1111/lam.13334
更新日期：2020-09-01 00:00:00
abstract：AIMS:The objective of this work was to purify the tyrosinase from Bacillusthuringiensis subsp. kurstaki (Bt) (CCTCC AB 90010) and study its enzymatic properties. METHODS AND RESULTS:A 'one-step' purification method was used in this work, which was an easy, high-yield purification method. Tyrosinase activity of this pu...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2004.01599.x
更新日期：2004-01-01 00:00:00
abstract：AIMS:The objective of this study was to determine the antifungal activity of flurbiprofen against dermatophytes like Trichophyton, Microsporum and Epidermophyton species. METHODS AND RESULTS:Susceptibility tests were performed against dermatophytes like Trichophyton, Microsporum and Epidermophyton species by the micro...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2003.01370.x
更新日期：2003-01-01 00:00:00
abstract：:Random amplification of polymorphic DNA (RAPD) is proving to be a useful technique in studying the epidemiology of micro-organisms. The technique can be troublesome and time consuming to establish due to the essentially empirical approach to optimization. By standardization of certain parameters and use of a commercia...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.1997.00385.x
更新日期：1997-04-01 00:00:00
abstract：AIMS:The use of randomly amplified polymorphic DNA (RAPD)-PCR fingerprinting and plasmid profiles to determine at the strain level, the similarity of Lactococcus lactis isolates obtained during sampling of traditional cheeses and to verify its correspondence to the selected phenotypic characteristics. METHODS AND RESU...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2006.02012.x
更新日期：2006-12-01 00:00:00
abstract：AIMS:The aim of this study was to investigate the role of divercin V41 in inhibition and prevention of Listeria monocytogenes. METHODS AND RESULTS:Carnobacterium divergens V41 deficient in bacteriocin production was isolated and characterized by enzyme-liked immunosorbent assay, multiplex polymerase chain reaction and...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2003.01310.x
更新日期：2003-01-01 00:00:00
abstract：AIMS:The aim of this study was to detect and quantify DNA of the ochratoxinogenic fungus Aspergillus ochraceus in green coffee and to compare the results with the ochratoxin A content of naturally contaminated samples. METHODS AND RESULTS:A DNA extraction protocol based on a combination of ultrasonification and a comm...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2004.01524.x
更新日期：2004-01-01 00:00:00
abstract：:The effect of liquid mechanical vibration on the adhesion of Saccharomyces cerevisiae cells to the internal glass surface of a pipette was studied using a 25 Hz vibration source. The maximum vibration amplitude was 1.06 mm (peak to peak) along the pipette direction. Relative movements between the pipette and yeast sus...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.1996.tb00022.x
更新日期：1996-07-01 00:00:00
abstract：AIMS:The genetic diversity of Vibrio cholerae isolated from black tiger shrimp (Penaeus monodon) aquaculture farms was determined using three PCR typing methods based on enterobacterial repetitive intergenic consensus (ERIC) sequences, ribosomal gene spacer (RS) sequence and repetitive extragenic palindromic (REP) sequ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2010.02863.x
更新日期：2010-07-01 00:00:00
abstract：AIMS:To develop and evaluate a real-time PCR assay incorporating an internal amplification control (IAC) suitable for the screening of Shiga toxin (Stx)-producing Escherichia coli (STEC) in foods. METHODS AND RESULTS:A competitive IAC was constructed and included in an stx-specific real-time PCR assay. Coupled to 18-h...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2009.02561.x
更新日期：2009-05-01 00:00:00
abstract：AIMS:To test the efficacy of Lactobacillus johnsonii FI9785 in reducing the colonization and shedding of Salmonella enterica serotype Enteritidis, Escherichia coli O78:K80 and Clostridium perfringens in poultry. METHODS AND RESULTS:Specific pathogen-free chicks (1 day old) were dosed with a single oral inoculum of 1x1...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.2004.01474.x
更新日期：2004-01-01 00:00:00
abstract：AIMS:To examine factors affecting faecal shedding of the foodborne pathogens Escherichia coli O157:H7 and Salmonella in dairy cattle and evaluate antimicrobial susceptibility of these isolates. METHODS:Faecal samples were obtained in replicate from lactating (LAC; n = 60) and non-lactating (NLAC; n = 60) Holstein catt...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2003.01417.x
更新日期：2003-01-01 00:00:00
abstract：UNLABELLED:Four strains were isolated from a spoiled whiting (Merlangius merlangus). All of them were able to grow aerobically from 4 to 30°C and also able to develop anaerobically in the presence of trimethylamine N-Oxide (TMAO) at 25°C. Biochemical characterization did not allow identification of the strains species ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12312
更新日期：2014-11-01 00:00:00
