Induction of intestinal IgA and IgG antibodies preventing adhesion of verotoxin-producing Escherichia coli to Caco-2 cells by oral immunization with liposomes.

abstract：AIMS:To isolate and identify diazotrophic endophytes in the stem of Japanese sweetpotato cv. Koganesengan. METHODS AND RESULTS:Surface-sterilized and thinly sliced (1-2 mm) sweetpotato stem samples were incubated in test tubes with semi-solid modified Rennie (MR) medium. The test tubes were assayed for acetylene reduc...

journal_title：Letters in applied microbiology

authors： Asis CA Jr,Adachi K

更新日期：2004-01-01 00:00:00

abstract：:Leuconostoc strains were characterized according to their antibiotic susceptibilities, carbohydrate fermentation profiles, sucrase activity patterns and plasmid content. All the strains tested were resistant to the antibiotics sulphathiazole, trimethoprim and vancomycin, and could be separated into two groups based on...

journal_title：Letters in applied microbiology

authors： Holt SM,Al-Sheikh H,Shin KJ

更新日期：2001-03-01 00:00:00

abstract：AIM:To investigate the viability, surface characteristics and ability of spores of a Geobacillus sp. isolated from a milk powder production line to adhere to stainless steel surfaces before and after a caustic (NaOH) wash used in clean-in-place regimes. METHODS AND RESULTS:Exposing sessile spores to 1% NaOH at 65°C fo...

journal_title：Letters in applied microbiology

authors： Brent Seale R,Flint SH,James McQuillan A,Bremer PJ

更新日期：2011-02-01 00:00:00

abstract：:A set of Escherichia coli freshwater isolates was chosen to compare the effectiveness of denaturing gradient gel electrophoresis (DGGE) vs temporal temperature gradient gel electrophoresis (TTGE) for separating homologous amplicons from the respective uidA region differing in one to seven single base substitutions. Bo...

journal_title：Letters in applied microbiology

authors： Farnleitner AH,Kreuzinger N,Kavka GG,Grillenberger S,Rath J,Mach RL

更新日期：2000-06-01 00:00:00

abstract：AIMS:To investigate the use of a Staphylococcus epidermidis transcriptional regulator gene as target for species-specific determination. METHODS AND RESULTS:Staph. epidermidis genes encoding putative transcriptional regulators were retrieved from GenBank and those showing no homology with other bacterial sequences wer...

journal_title：Letters in applied microbiology

authors： Liu D,Swiatlo E,Austin FW,Lawrence ML

更新日期：2006-09-01 00:00:00

abstract：AIMS:The effect of high organic loading rate (OLR) on the physical characteristics of aerobic granules was studied. METHODS AND RESULTS:Two column-type sequential aerobic sludge blanket reactors were fed with either glucose or acetate as the main carbon source, and the OLR was gradually raised from 6 to 9, 12 and 15 k...

journal_title：Letters in applied microbiology

authors： Moy BY,Tay JH,Toh SK,Liu Y,Tay ST

更新日期：2002-01-01 00:00:00

abstract：AIMS:To develop a sensitive, rapid and simple method for detection of Botrytis cinerea based on loop-mediated isothermal amplification (LAMP) that would be suitable for use outside a conventional laboratory setting. METHODS AND RESULTS:A LAMP assay was designed based on the intergenic spacer of the B. cinerea nuclear ...

journal_title：Letters in applied microbiology

authors： Tomlinson JA,Dickinson MJ,Boonham N

更新日期：2010-12-01 00:00:00

abstract：AIMS:Strains of Clostridium butyricum have been increasingly used as probiotics for both animals and humans. The aim of this study was to develop a growth medium for cultivating C. butyricum ZJUCB using a statistical methodology. METHODS AND RESULTS:Response surface methodology (RSM) was used to evaluate the effects o...

journal_title：Letters in applied microbiology

authors： He GQ,Kong Q,Ding LX

更新日期：2004-01-01 00:00:00

abstract：UNLABELLED:Isolation of local strains is mandatory for the success of control programs. However, clinical samples are typically contaminated by other bacteria, which impair leptospires growth. The purpose of this study was to evaluate the use of a previously reported EMJH-STAFF media in the recovery of pathogenic lepto...

journal_title：Letters in applied microbiology

authors： Loureiro AP,Martins G,Pinto P,Narduche L,Teixeira RC,Lilenbaum W

更新日期：2015-12-01 00:00:00

abstract：AIMS:The aim of this study is to develop an RT-PCR assay combined with immunomagnetic beads (IMS/RT-PCR) coating monoclonal antibody (Mab) for separation and detection of norovirus (genogroup II) in faecal samples. We furthermore compare its detection limits with IMS/RT-PCR using polyclonal antibody (Pab) and the TRIzo...

journal_title：Letters in applied microbiology

authors： Yao L,Wu Q,Wang D,Kou X,Zhang J

更新日期：2009-08-01 00:00:00

abstract：AIMS:To characterize the bacterial communities in commercial total mixed ration (TMR) silage, which is known to have a long bunk life after silo opening. METHODS AND RESULTS:Samples were collected from four factories that produce TMR silage according to their own recipes. Three factories were sampled three times at 1-...

journal_title：Letters in applied microbiology

authors： Wang C,Nishino N

更新日期：2010-10-01 00:00:00

abstract：AIMS:To measure the microbial removal capacity of a small-scale hydroponics wastewater treatment plant. METHODS AND RESULTS:Paired samples were taken from untreated, partly-treated and treated wastewater and analysed for faecal microbial indicators, i.e. coliforms, Escherichia coli, enterococci, Clostridium perfringen...

journal_title：Letters in applied microbiology

authors： Ottoson J,Norström A,Dalhammar G

更新日期：2005-01-01 00:00:00

abstract：AIMS:Natural and synthetic antimicrobial peptides (AMPs) are of increasing interest as potential resistance conferring elements in plants against pathogen infection. The efficacy of AMPs against pathogens is prescreened by in vitro assays, and promising AMP candidates are introduced as transgenes into plants. As in vit...

journal_title：Letters in applied microbiology

authors： Visser M,Stephan D,Jaynes JM,Burger JT

更新日期：2012-06-01 00:00:00

abstract：:The contribution of trehalose and hsp104 to barotolerance in Saccharomyces cerevisiae has been investigated. Mutant strains, which lacked the ability to accumulate trehalose and/or hsp104, were examined for barotolerance and thermotolerance. All the mutants showed lower barotolerance and thermotolerance than their con...

journal_title：Letters in applied microbiology

authors： Iwahashi H,Obuchi K,Fujii S,Komatsu Y

更新日期：1997-07-01 00:00:00

abstract：:Sodium pyrithione and zinc pyrithione (NaPT and ZnPT, respectively) are widely used as cosmetic preservatives and metal chelating agents. They are commonly assayed using thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). However, a simple quantitative colorimetric assay has not been pre...

journal_title：Letters in applied microbiology

authors： Dinning AJ,al-Adham IS,Austin P,Collier PJ

更新日期：1998-07-01 00:00:00

abstract：:A Multiplex PCR-based assay (m-PCR) with three sets of primers was developed for the detection of all serotypes of Salmonella enterica and the identification of Salmonella Enteritidis and Salmonella Typhimurium. This method was evaluated against a bacteriological method for the analysis of environmental swabs of poult...

journal_title：Letters in applied microbiology

authors： Soumet C,Ermel G,Rose N,Rose V,Drouin P,Salvat G,Colin P

更新日期：1999-02-01 00:00:00

abstract：AIMS:The purpose of this study was to determine if DNA polymorphisms generated by RAPD-PCR could be used to characterize Group B streptococci (GBS) for epidemiological purposes. METHODS AND RESULTS:30 unrelated, previously serotyped strains were analysed by RAPD-PCR using two 10-mer primers (5' TGCGAGAGTC 3' and 5' AG...

journal_title：Letters in applied microbiology

authors： Zhang GW,Kotiw M,Daggard G

更新日期：2002-01-01 00:00:00

abstract：UNLABELLED:We investigated the role of the Bradyrhizobium japonicum lpcC gene, encoding a mannosyl transferase, involved in the lipopolysaccharide (LPS) biosynthesis. The inactivation of the lpcC gene considerably altered the LPS structure and the cell surface properties. LPS analysis showed that the lpcC mutant JS715 ...

journal_title：Letters in applied microbiology

authors： Lee HI,In YH,Jeong SY,Jeon JM,Noh JG,So JS,Chang WS

更新日期：2014-07-01 00:00:00

abstract：:Irreversible electroporation (IRE) damages cell membranes and is used in medicine for nonthermal ablation of malignant tumours. Our aim was to evaluate the antimicrobial effect of IRE. The pathogenic micro-organisms, Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa and Candida al...

journal_title：Letters in applied microbiology

authors： Korem M,Goldberg NS,Cahan A,Cohen MJ,Nissenbaum I,Moses AE

更新日期：2018-07-01 00:00:00

abstract：UNLABELLED:Bacterial vaginosis is a frequent dysbiosis, where the normal lactobacillus-dominated flora is replaced by an anaerob/aerob polymicrobial flora. Bacterial vaginosis increases the risk of acquiring sexually transmitted infections (STI) including the most frequent Chlamydia trachomatis infections. Intravaginal...

journal_title：Letters in applied microbiology

authors： Párducz L,Eszik I,Wagner G,Burián K,Endrész V,Virok DP

更新日期：2016-10-01 00:00:00

abstract：AIMS:The optimal culture conditions for endoinulinase production using chicory roots were studied in shake-flask culture. METHODS AND RESULTS:Much higher enzyme production was achieved with Xanthomonas sp. (15 U ml(-1)) than with Pseudomonas sp. (3 U ml(-1)). Optimized culture conditions of Xanthomonas sp. for endoinu...

journal_title：Letters in applied microbiology

authors： Park JP,Yun JW

更新日期：2001-09-01 00:00:00

abstract：AIMS:Our goal was to develop a mathematical kinetic model to predict the sporicidal activity of glutaraldehyde, which is an active ingredient frequently used in commercial products employed for liquid disinfection and decontamination. METHODS AND RESULTS:We used our previously published data on spore inactivation by g...

journal_title：Letters in applied microbiology

authors： Retta SM,Sagripanti JL

更新日期：2008-05-01 00:00:00

abstract：AIMS:To develop and evaluate a new and reliable real-time PCR detection protocol on chromosomal DNA of the contagious plant pathogenic bacterium Erwinia amylovora, the causal agent of fire blight. METHODS AND RESULTS:A Taqman minor-groove-binder real-time PCR assay targeting a hypothetical protein coding gene of Erw. ...

journal_title：Letters in applied microbiology

authors： Gottsberger RA

更新日期：2010-09-01 00:00:00

abstract：AIM:The study aimed to evaluate the effect of the bacteriocins produced by Lactobacillus sakei CWBI-B1365 and Lactobacillus curvatus CWBI-B28 on the growth and survival of Listeria monocytogenes in raw beef and poultry meat. METHODS AND RESULTS:The sakacin P and sakacin G structural genes were identified in Lact. curv...

journal_title：Letters in applied microbiology

authors： Dortu C,Huch M,Holzapfel WH,Franz CM,Thonart P

更新日期：2008-12-01 00:00:00

abstract：:Results of biocide efficacy testing on laboratory-generated microbial biofilms containing Legionella bozemanii is presented. These show that chlorine is effective at recommended concentrations in cooling towers; at least 48 h contact between free chlorine and the biofilm is necessary. Of five commercial biocides teste...

journal_title：Letters in applied microbiology

authors： Green PN

更新日期：1993-10-01 00:00:00

abstract：:Currently used alum precipitated and oil adjuvant vaccines against HS caused by Pasteurella multocida B:2, have side effects and short-lived immunity, leading to regular catastrophic outbreaks in bovines in Asian subcontinent. The need for the development of an improved vaccine with longer immunity and the ability to ...

journal_title：Letters in applied microbiology

authors： Qureshi S,Saxena HM,Imam N,Kashoo Z,Sharief Banday M,Alam A,Malik MZ,Ishrat R,Bhat B

更新日期：2018-09-01 00:00:00

abstract：:The Andrias davidianus has been known as a traditional Chinese medicine for a long time. Its blood is considered as a waste or by-product of the meat production industry. Although there are reports on isolation of the antimicrobial peptides from different resources, there are no reports of their isolation from A. davi...

journal_title：Letters in applied microbiology

authors： Pei J,Feng Z,Ren T,Sun H,Han H,Jin W,Dang J,Tao Y

更新日期：2018-01-01 00:00:00

abstract：:Emulsions of the fatty acids linoleic (C18:2 n-6), alpha-linolenic (C18:3 n-3) and arachidonic acid (C20:4 n-6) were incubated for 4 h under anaerobic conditions with human faecal suspensions. Linoleic acid was significantly decreased (P < 0.001) and there was a significant rise (P < 0.05) in its hydrogenation product...

journal_title：Letters in applied microbiology

authors： Howard FA,Henderson C

更新日期：1999-09-01 00:00:00

abstract：:A 24 h period in waste waters improved the subsequent survival of Salmonella in oligotrophic sea water, at 20 degrees C, compared to a direct input control. The main osmoprotective compound accumulated, investigated by 1H-NMR spectroscopy (nuclear magnetic resonance), after 6 d in sea water was trehalose. Taking into ...

journal_title：Letters in applied microbiology

authors： Dupray E,Derrien A,Pichon R

更新日期：1995-03-01 00:00:00

abstract：:Investigations of a free-range broiler flock during the rearing period and at the slaughterhouse by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the flagellin (flaA) gene (flaA typing) have shown that poultry carcasses are contaminated by Campylobacter spp. strains which were previo...

journal_title：Letters in applied microbiology

authors： Rivoal K,Denis M,Salvat G,Colin P,Ermel G

更新日期：1999-12-01 00:00:00