Abstract:
:Sodium pyrithione and zinc pyrithione (NaPT and ZnPT, respectively) are widely used as cosmetic preservatives and metal chelating agents. They are commonly assayed using thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). However, a simple quantitative colorimetric assay has not been previously reported for these compounds. This paper describes the development of a spectrophotometric assay for the quantification of the pyrithiones which is based on the chelation of copper (II) ions by the biocides. This assay was developed in order to facilitate the determination of the distribution of these biocides in the Gram-negative bacteria Escherichia coli NCIMB 10,000 and Pseudomonas aeruginosa NCIMB 10,548. Sodium pyrithione was exhibited only in the cytosol of E. coli and Ps. aeruginosa. Zinc pyrithione, however, was assayed in the cytosol of both bacteria and was found in the cell envelope of Ps. aeruginosa. These findings suggest that the pyrithione biocides are active within bacterial cells as well as at the cell membrane.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Dinning AJ,al-Adham IS,Austin P,Collier PJdoi
10.1046/j.1472-765x.1998.00376.xsubject
Has Abstractpub_date
1998-07-01 00:00:00pages
1-4issue
1eissn
0266-8254issn
1472-765Xjournal_volume
27pub_type
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