Effect of NaOH (caustic wash) on the viability, surface characteristics and adhesion of spores of a Geobacillus sp. isolated from a milk powder production line.

Abstract:

AIM:To investigate the viability, surface characteristics and ability of spores of a Geobacillus sp. isolated from a milk powder production line to adhere to stainless steel surfaces before and after a caustic (NaOH) wash used in clean-in-place regimes. METHODS AND RESULTS:Exposing sessile spores to 1% NaOH at 65°C for 30min decreased spore viability by two orders of magnitude. The zeta potential of the caustic treated spores decreased from -20 to -32 mV and they became more hydrophobic. Transmission electron microscopy revealed that caustic treated spores contained breaks in their spore coat. Under flow conditions, caustic treated spores suspended in 0·1 mol l(-1) KCl were shown to attach to stainless steel in significantly greater numbers (4·6 log(10)  CFU cm(-2) ) than untreated spores (3·6 log(10)  CFU cm(-2) ). CONCLUSIONS:  This research suggests that spores surviving a caustic wash will have a greater propensity to attach to stainless steel surfaces. SIGNIFICANCE OF STUDY:  The practice of recycling caustic wash solutions may increase the risk of contaminating dairy processing surfaces with spores.

journal_name

Lett Appl Microbiol

authors

Brent Seale R,Flint SH,James McQuillan A,Bremer PJ

doi

10.1111/j.1472-765X.2010.02970.x

subject

Has Abstract

pub_date

2011-02-01 00:00:00

pages

104-8

issue

2

eissn

0266-8254

issn

1472-765X

journal_volume

52

pub_type

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