Detection of Botrytis cinerea by loop-mediated isothermal amplification.

abstract：:The novel Eriocheir sinensis reovirus (EsRV) is a pathogen that causes severe disease and high mortality rates in cultivated crabs. Here, we established a highly sensitive and specific rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that was cheaper and more suitable for field applic...

journal_title：Letters in applied microbiology

authors： Ma Y,Dai T,Serwadda A,Shen H

更新日期：2016-11-01 00:00:00

abstract：AIMS:To determine whether nonspore-forming biothreat agents can survive and persist in potable water that does not contain a disinfectant. METHODS AND RESULTS: Autoclaved, de-chlorinated Atlanta municipal water was inoculated with eight isolates of bacterial biothreat agents (10⁶ CFU ml⁻¹). The inoculated water sample...

journal_title：Letters in applied microbiology

authors： Gilbert SE,Rose LJ

更新日期：2012-09-01 00:00:00

abstract：:Microsomal chitinase from yeast and hyphal cells of Candida albicans was activated endogenously by incubation at 30 degrees C and exogenously by trypsin. The putative activating factor of yeast cells was separated from chitinase activity by fractionation of lysed protoplasts on an Iodixanol density gradient. The vacuo...

journal_title：Letters in applied microbiology

authors： Jackson DJ,Saunders VA,Humphreys AM

更新日期：1996-09-01 00:00:00

abstract：:Colistin-resistant Pseudomonas aeruginosa (CRPA), as a health care system threat, is increasing globally. This study aimed was to determine CRPA by high-resolution melting curve (HRM) analysis method. The HRM method was done on standard strains of P. aeruginosa and CRPA strains. Ninefold serial dilutions of known geno...

journal_title：Letters in applied microbiology

authors： Tahmasebi H,Dehbashi S,Arabestani MR

更新日期：2020-04-01 00:00:00

abstract：UNLABELLED:Palm wine produced traditionally and consumed by many people in the South-West of Burkina Faso is subject to alteration. In this study, we carried out a follow-up of two palm wines' fermentation during the 10 days in which palm wines are classically produced and consumed. We monitored biochemical characteris...

journal_title：Letters in applied microbiology

authors： Tapsoba F,Savadogo A,Legras JL,Zongo C,Traore AS

更新日期：2016-10-01 00:00:00

abstract：AIMS:The aim of this study was to determine the antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20 against several micro-organisms, including Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi. METHODS AND RESULTS:Antimicrobial and ...

journal_title：Letters in applied microbiology

authors： Gudiña EJ,Rocha V,Teixeira JA,Rodrigues LR

更新日期：2010-04-01 00:00:00

abstract：AIMS:Adaptation of a colorimetric assay using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) to evaluate the cytotoxicity of the extracellular products of micro-organisms pathogenic to fish. METHODS AND RESULTS:The optimal conditions for the colorimetric assay were determined and this method was...

journal_title：Letters in applied microbiology

authors： Zorrilla I,Balebona MC,Moriñigo MA

更新日期：2001-11-01 00:00:00

abstract：:Actinomyces viscosus strain GA produces an exocellular biological retardant(s) that prevents certain vegetable plants from becoming overgrown. The biological retardant(s) was assayed using the etiolated wheat coleoptile assay, and fractionation of culture supernatant fluid resulted in a partial purification of the ret...

journal_title：Letters in applied microbiology

authors： Bryant FO,Cutler HG

更新日期：1997-08-01 00:00:00

abstract：UNLABELLED:In this study, we identified five strains isolated from soil and sediments able to degrade kraft lignin, aromatic dyes and lignin derivatives. Using 16S rRNA gene sequencing, the isolates were identified as Serratia sp. JHT01, Serratia liquefacien PT01, Pseudomonas chlororaphis PT02, Stenotrophomonas maltoph...

journal_title：Letters in applied microbiology

authors： Tian JH,Pourcher AM,Peu P

更新日期：2016-07-01 00:00:00

abstract：AIMS:The study was carried out to evaluate the use of randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) as a method for the identification of lactobacilli isolated from meat products. METHODS AND RESULTS:RAPD-PCR with primers M13 and D8635 was applied to the identification and intraspecific diffe...

journal_title：Letters in applied microbiology

authors： Andrighetto C,Zampese L,Lombardi A

更新日期：2001-07-01 00:00:00

abstract：:Anti-MRSA activity of sophoraflavanone G (SFG) and synergism between SFG and antibacterial agents against MRSA (methicillin-resistant Staphylococcus aureus) were evaluated by means of Minimal Inhibitory Concentrations (MIC). The MICs of SFG against 27 strains of MRSA ranged from 3.13 to 6.25 micrograms ml-1. Synergism...

journal_title：Letters in applied microbiology

authors： Sakagami Y,Mimura M,Kajimura K,Yokoyama H,Linuma M,Tanaka T,Ohyama M

更新日期：1998-08-01 00:00:00

abstract：AIMS:In traditional Thai medicine, nutgall of Quercus infectoria G. Olivier is well-documented as an effective agent for wound and skin infections. The present study was aimed to establish modes of action of the ethanol extract of the plant as well as its main constituents to induce anti-methicillin-resistant Staphyloc...

journal_title：Letters in applied microbiology

authors： Chusri S,Voravuthikunchai SP

更新日期：2011-06-01 00:00:00

abstract：:In this study, a total of 172 putative omega-3 producers were isolated from 28 sediment samples from the Arabian Gulf employing a selective isolation procedure using marine agar containing 0·1% triphenyl tetrazolium chloride (TTC). Out of these 172 isolates, 19 isolates produced eicosapentaenoic acid (EPA) as confirme...

journal_title：Letters in applied microbiology

authors： Alagarsamy S,Sabeena Farvin KH,Fakhraldeen S,Kooramattom MR,Al-Yamani F

更新日期：2019-08-01 00:00:00

abstract：AIMS:Natural and synthetic antimicrobial peptides (AMPs) are of increasing interest as potential resistance conferring elements in plants against pathogen infection. The efficacy of AMPs against pathogens is prescreened by in vitro assays, and promising AMP candidates are introduced as transgenes into plants. As in vit...

journal_title：Letters in applied microbiology

authors： Visser M,Stephan D,Jaynes JM,Burger JT

更新日期：2012-06-01 00:00:00

abstract：AIMS:To determine the underlying substrate utilization mechanism in the logistic equation for batch microbial growth by revealing the relationship between the logistic and Monod kinetics. Also, to determine the logistic rate constant in terms of Monod kinetic constants. METHODS AND RESULTS:The logistic equation used t...

journal_title：Letters in applied microbiology

authors： Kargi F

更新日期：2009-04-01 00:00:00

abstract：:Salmonella enteritidis-inoculated poultry sausages were pressurized at 500 MPa by combining different times (10 and 30 min) and temperatures (50, 60 and 70 degrees C) or heat treated with the same temperature-time combinations and a standard cooking (75 degrees C for 30 min). Counts of Salm. enteritidis and mesophilic...

journal_title：Letters in applied microbiology

authors： Yuste J,Pla R,Mor-Mur M

更新日期：2000-11-01 00:00:00

abstract：:The genus Taylorella is composed of two species: (i) Taylorella equigenitalis, the causative agent of CEM, a venereally transmitted infection of Equidae and (ii) Taylorella asinigenitalis, a closely related species considered to be nonpathogenic, although experimental infection of mares with this bacterium resulted in...

journal_title：Letters in applied microbiology

authors： Hébert L,Rincé I,Sanna C,Laugier C,Rincé A,Petry S

更新日期：2014-10-01 00:00:00

abstract：:The effect of liquid mechanical vibration on the adhesion of Saccharomyces cerevisiae cells to the internal glass surface of a pipette was studied using a 25 Hz vibration source. The maximum vibration amplitude was 1.06 mm (peak to peak) along the pipette direction. Relative movements between the pipette and yeast sus...

journal_title：Letters in applied microbiology

authors： Jiang C,Yu H,Ding Z,Ma Y

更新日期：1996-07-01 00:00:00

abstract：AIMS:To provide comparative genome sequence data for two related model strains of Staphylococcus aureus (SH1000 and 8325-4) that are used extensively in laboratory research. METHODS AND RESULTS:Comparative genome sequencing was used to identify genetic differences between Staph. aureus SH1000 and the fully genome-sequ...

journal_title：Letters in applied microbiology

authors： O'Neill AJ

更新日期：2010-09-01 00:00:00

abstract：AIMS:Breast milk has been described as a source of bacteria influencing the development of the infant gut microbiota. Up to the present, few studies have been focused on the application of culture-independent techniques to study bacterial diversity in breast milk. In this context, the aim of this study was to character...

journal_title：Letters in applied microbiology

authors： Collado MC,Delgado S,Maldonado A,Rodríguez JM

更新日期：2009-05-01 00:00:00

abstract：AIMS:To assess the effectiveness of sequential treatments of radish seeds with aqueous chlorine dioxide (ClO(2) ) and dry heat in reducing the number of Escherichia coli O157:H7. METHODS AND RESULTS:Radish seeds containing E. coli O157:H7 at 5.5 log CFU g(-1) were treated with 500 μg ml(-1) ClO(2) for 5 min and subseq...

journal_title：Letters in applied microbiology

authors： Bang J,Kim H,Kim H,Beuchat LR,Kim Y,Ryu JH

更新日期：2011-10-01 00:00:00

abstract：AIM:The study aimed to evaluate the effect of the bacteriocins produced by Lactobacillus sakei CWBI-B1365 and Lactobacillus curvatus CWBI-B28 on the growth and survival of Listeria monocytogenes in raw beef and poultry meat. METHODS AND RESULTS:The sakacin P and sakacin G structural genes were identified in Lact. curv...

journal_title：Letters in applied microbiology

authors： Dortu C,Huch M,Holzapfel WH,Franz CM,Thonart P

更新日期：2008-12-01 00:00:00

abstract：AIMS:To provide evidence that DNA-PCR diagnostics of oral pathogens based on standard sample collection by paper point insertion from the depth of the periodontal pocket can be replaced by a novel non-invasive collection method based on swab technique from the gingiva. METHODS AND RESULTS:In this study we compared the...

journal_title：Letters in applied microbiology

authors： Smola SF,Rettenberger G,Simmet T,Burysek L

更新日期：2003-01-01 00:00:00

abstract：:Irreversible electroporation (IRE) damages cell membranes and is used in medicine for nonthermal ablation of malignant tumours. Our aim was to evaluate the antimicrobial effect of IRE. The pathogenic micro-organisms, Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa and Candida al...

journal_title：Letters in applied microbiology

authors： Korem M,Goldberg NS,Cahan A,Cohen MJ,Nissenbaum I,Moses AE

更新日期：2018-07-01 00:00:00

abstract：AIMS:To investigate a lactococcal plasmid responsible for a reduction in growth rate of its host cell. METHODS AND RESULTS:Lactococcus lactis subsp. lactis biovar. diacetylactis DRC1 carries a high number of plasmids. The DRC1 wild-type strain was found to grow more slowly than a plasmid-free derivative of DRC1. The p...

journal_title：Letters in applied microbiology

authors： Kobayashi M,Nomura M,Fujita Y,Okamoto T,Ohmomo S

更新日期：2002-01-01 00:00:00

abstract：AIMS:The development of a simple, rapid and sensitive method for the screening of chitinase inhibitors using Ostazin Brilliant Red labelled chitin as a substrate for chitinase assay. METHODS AND RESULTS:The colloidal substrate prepared from the Ostazin Brilliant Red labelled chitin was used in the estimation of chitin...

journal_title：Letters in applied microbiology

authors： Shirazi F,Kulkarni M,Deshpande MV

更新日期：2007-06-01 00:00:00

abstract：UNLABELLED:Lactobacillus acidophilus LAB20 has potential to be a probiotic strain because it can be present at high numbers in the jejunum of dog. To specifically detect LAB20 from dog faecal samples, a real-time PCR protocol was developed targeting the novel surface (S) layer protein gene of LAB20. The presence of S-l...

journal_title：Letters in applied microbiology

authors： Tang Y,Saris PE

更新日期：2013-10-01 00:00:00

abstract：AIMS:Aims of investigation: (i) develop a quantitative RT-PCR for noroviruses and (ii) evaluate it on environmental samples. METHODS AND RESULTS:Noroviruses in environmental water samples were concentrated by adsorption/elution/flocculation. Sewage was processed by clarification and protein flocculation. Norovirus-spe...

journal_title：Letters in applied microbiology

authors： Laverick MA,Wyn-Jones AP,Carter MJ

更新日期：2004-01-01 00:00:00

abstract：:Growth at low positive temperatures and induced phenotypic resistance to extreme cold temperature (freezing/thawing cycles) of Enterococcus faecalis were investigated. The effect of low temperatures on the specific growth rates was studied; use of Arrhenius profile and Ratkovsky 'square-root' model allowed determinati...

journal_title：Letters in applied microbiology

authors： Thammavongs B,Corroler D,Panoff JM,Auffray Y,Boutibonnes P

更新日期：1996-12-01 00:00:00

abstract：AIMS:  To show that application of copper hydroxide citrus sprays mixed with field source water (possibly contaminated) will not support Escherichia coli on plant surfaces. Environmental stresses of transient phyllosphere bacteria and presence of copper will eradicate these bacteria before harvest. METHODS AND RESULTS...

journal_title：Letters in applied microbiology

authors： Narciso JA,Ference CM,Ritenour MA,Widmer WW

更新日期：2012-02-01 00:00:00