Influence of lactococcal plasmid on the specific growth rate of host cells.

Abstract:

AIMS:To investigate a lactococcal plasmid responsible for a reduction in growth rate of its host cell. METHODS AND RESULTS:Lactococcus lactis subsp. lactis biovar. diacetylactis DRC1 carries a high number of plasmids. The DRC1 wild-type strain was found to grow more slowly than a plasmid-free derivative of DRC1. The plasmids extracted from DRC1 together with an indicator plasmid were cotransformed into the plasmid-free strain DRC1021. A 7.4-kb cryptic plasmid, designated pDR1-1, was found to significantly affect the maximum specific growth rate ( micro max) of the host cell. Polymerase chain reaction (PCR) analysis was carried out in order to detect the presence of pDR1-1 in the other L. lactis strains. The micro max of the single pDR1-1-positive strain was determined to be the same as that of DRC1. SIGNIFICANCE AND IMPACT OF THE STUDY:These results suggest that pDR1-1 (or a pDR1-1-like plasmid) is a critical factor in the reduction of the micro max of DRC1, and that its effect on the micro max is significantly greater than that of any other coexisting plasmid.

journal_name

Lett Appl Microbiol

authors

Kobayashi M,Nomura M,Fujita Y,Okamoto T,Ohmomo S

doi

10.1046/j.1472-765x.2002.01213.x

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

403-8

issue

5

eissn

0266-8254

issn

1472-765X

pii

1213

journal_volume

35

pub_type

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