Biocatalysis with Escherichia coli-overexpressing cyclopentanone monooxygenase immobilized in polyvinyl alcohol gel.

Abstract:

UNLABELLED:This is the first reported study on the immobilization of living recombinant Escherichia coli cells that overexpress cyclopentanone monooxygenase in polyvinyl alcohol gel particles LentiKats®. Immobilized cells overexpressing cyclopentanone monooxygenase have been used as a model of biocatalyst for enantioselective Baeyer-Villiger biooxidation of rac-bicyclo[3.2.0]hept-2-en-6-one into regioisomeric lactones. This process is useful for the syntheses of cytostatic sarkomycin, several prostaglandins and other biologically active compounds. The original technique for qualitative analysis of enzyme expression within free cells and cells entrapped in LentiKats® using SDS-PAGE was developed and used for verification of optimal conditions for the induction of cyclopentanone monooxygenase. Here, we successfully performed six repeated batch Baeyer-Villiger biooxidations utilizing entrapped cells using 40% (w/v) polyvinyl alcohol gel particles in flasks with baffles. The latter conditions have been found to be the most appropriate achieving optimal oxygen transfer within LentiKats®. Moreover, immobilized cells retained their catalytic efficiency over six reaction cycles, while the catalytic efficiency of free cells decreased after three reaction cycles. SIGNIFICANCE AND IMPACT OF THE STUDY:Immobilization in polyvinylalcohol gel particles is desirable technique with presumptive impact on industrial applications of recombinant whole-cell Baeyer-Villiger monooxygenases as biocatalysts for production of bioactive compounds and precursors of potentially new drugs. An original immobilization of cells E. coli with overproduced Baeyer-Villiger monooxygenase improved their stability in repetitive batch biooxidations as compared to free cells. Detected autoinduction of recombinant enzyme in pET22b+ plays significant role in application of immobilized cells as it may increase specific activity of cells in repetitive use under growing reaction conditions. Original technique for qualitative analysis of enzyme expression within immobilized cells was developed.

journal_name

Lett Appl Microbiol

authors

Rebroš M,Lipták L,Rosenberg M,Bučko M,Gemeiner P

doi

10.1111/lam.12227

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

556-63

issue

6

eissn

0266-8254

issn

1472-765X

journal_volume

58

pub_type

杂志文章
  • Comparison of bioMérieux API 20NE and Remel RapID NF Plus, identification systems of type strains of Ralstonia pickettii.

    abstract:UNLABELLED:A : Comparison of two commercial miniaturized rapid systems for the identification of Ralstonia pickettii strains. METHODS AND RESULTS:Varying identification results were encountered using the bioMérieux API NE system and the Remel IDS RapID NF Plus commercial systems for R. pickettii. To compare these two ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2005.01737.x

    authors: Adley CC,Saieb FM

    更新日期:2005-01-01 00:00:00

  • Glucose metabolism and internal pH of Lactococcus lactis subsp. lactis cells utilizing NMR spectroscopy.

    abstract::The metabolism of glucose was studied in Lactococcus lactis subsp. CNRZ 125 by 13C NMR. The initial rate of glucose utilization was higher for exponential phase cells than for stationary phase cells [150 vs 85 nmol g (dry wt)-1 s -1]. 31P NMR was used to determine changes in glycolytic phosphorylated intermediates (fr...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1995.tb00994.x

    authors: Foucaud C,Herve M,Neumann JM,Hemme D

    更新日期:1995-07-01 00:00:00

  • Faecal fatty acids and gastrointestinal upset in newborn infants.

    abstract::Patterns of long-chain faecal fatty acids were studied by gas-liquid chromatography in 55 newborn infants in a neonatal intensive care unit. Decreased fractions of fatty acid C16:1 and increased fractions of C16:0 and C17:0 were associated with the occurrence of abdominal distension. Decreased fractions of C16:1 and C...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1997.00341.x

    authors: Ahtonen P,Lehtonen OP,Kero P,Eerola E

    更新日期:1997-02-01 00:00:00

  • Rapid RAPD analysis for distinguishing Listeria species and Listeria monocytogenes serotypes using a capillary air thermal cycler.

    abstract::Using a novel capillary thermal cycler, randomly amplified polymorphic DNA (RAPD) generated DNA fingerprints were obtained in 3 h. The RAPD profiles were produced using a random 10-mer primer (5'-ACCGCCTGCT-3') which discriminated between different Listeria spp. Unique fragment profiles of Listeria monocytogenes serot...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1995.tb00424.x

    authors: Black SF,Gray DI,Fenlon DR,Kroll RG

    更新日期:1995-03-01 00:00:00

  • The incidence, numbers and types of Listeria monocytogenes isolated from farm bulk tank milks.

    abstract::Bulk tank milk from 160 producers was tested for Listeria monocytogenes at three monthly intervals over 1 year. Twenty-five producers were positive, most on a single occasion, only seven were positive on three or more of the four samplings. Listeria monocytogenes numbers were low, usually < 1 ml-1, the highest was 35 ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1995.tb00407.x

    authors: Fenlon DR,Stewart T,Donachie W

    更新日期:1995-01-01 00:00:00

  • Implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry.

    abstract:AIMS:The present study describes the implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry. METHODS AND RESULTS:Real-time PCR with Salmonella invA-specific primers and a standard bacteriological method was applied to detect Salmonella in tetrathionate enrichment cult...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01036.x

    authors: Eyigor A,Carli KT,Unal CB

    更新日期:2002-01-01 00:00:00

  • Isolation and identification of bioactive substance 1-hydroxyphenazine from Pseudomonas aeruginosa and its antimicrobial activity.

    abstract::A strain named as Pseudomonas aeruginosa 2016NX1, which could produce phenazine and cereusitin, was isolated from the root of Millettia specisoa. Phenazines were extracted, isolated and purified by chloroform, thin-layer chromatography, column chromatography and high-performance liquid chromatography. Then the purifie...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13332

    authors: Liu TT,Ye FC,Pang CP,Yong TQ,Tang WD,Xiao J,Shang CH,Lu ZJ

    更新日期:2020-09-01 00:00:00

  • Kinetics and effects of trace elements and electron complexes on 2-keto-4-methylthiobutyric acid-dependent biosynthesis of ethylene in soil.

    abstract:AIMS:2-Keto-4-methylthiobutyric acid (KMBA) is an established intermediate in microbial biosynthesis of ethylene from methionine. This study demonstrates the kinetics and effects of trace elements and electron complexes on substrate (KMBA)-derived C2H4 biosynthesis in soil. METHODS AND RESULTS:We have previously repor...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2004.01590.x

    authors: Arshad M,Nazli ZH,Khalid A,Zahir ZA

    更新日期:2004-01-01 00:00:00

  • The effect of colony age on PCR fingerprinting.

    abstract::The pattern of DNA fragments produced by the polymerase chain reaction using arbitrary primers is strongly influenced by the age of the colonies sampled. We show that the number, clarity and reproducibility of the bands produced is optimal when very young colonies are sampled. ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1993.tb01467.x

    authors: Coutinho HL,Handley BA,Kay HE,Stevenson L,Beringer JE

    更新日期:1993-12-01 00:00:00

  • Silica-immobilized Methylobacterium sp. NP3 and Acinetobacter sp. PK1 degrade high concentrations of phenol.

    abstract:AIMS:To immobilize Methylobacterium sp. NP3 and Acinetobacter sp. PK1 to silica and determine the ability of the immobilized bacteria to degrade high concentrations of phenol. METHODS AND RESULTS:The phenol degradation activity of suspended and immobilized Methylobacterium sp. NP3 and Acinetobacter sp. PK1 bacteria wa...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2011.03019.x

    authors: Khongkhaem P,Intasiri A,Luepromchai E

    更新日期:2011-05-01 00:00:00

  • Peptide synthesis catalysed by a haloalkaliphilic serine protease from the archaeon Natrialba magadii (Nep).

    abstract:AIMS:Haloarchaeal proteases function optimally in high salt (low water activity); thus, they offer an advantage over the nonhalophilic counterparts as biocatalysts for protease-catalysed peptide synthesis. The haloalkaliphilic archaeon Natrialba magadii secretes a solvent-tolerant protease, Nep (Natrialba magadii extra...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2010.02955.x

    authors: Ruiz DM,Iannuci NB,Cascone O,De Castro RE

    更新日期:2010-12-01 00:00:00

  • High-level secretion of human bikunin from recombinant Pichia pastoris.

    abstract:UNLABELLED:Human bikunin is a glycoprotein that exhibits trypsin inhibitory activity against serine proteases, and is effective in clinic. However, limited productivity and high price of human bikunin retard its further application. In this study, a high-yield, low-cost process of recombinant human bikunin (rh-bikunin)...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12470

    authors: Yao M,Zhang J,Wang X

    更新日期:2015-10-01 00:00:00

  • A preliminary assessment of Bacillus anthracis spore inactivation using an electrochemically activated solution (ECASOL).

    abstract:AIM:To evaluate the efficacy of electrochemically activated solution (ECASOL) in decontaminating Bacillus anthracis Ames and Vollum 1B spores, with and without changing the source water hardness and final ECASOL pH. METHODS AND RESULTS:Five different ECASOL formulations were generated, in which the source water hardne...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.02002.x

    authors: Rogers JV,Ducatte GR,Choi YW,Early PC

    更新日期:2006-11-01 00:00:00

  • Effect of environmental stresses on the sensitivity of Enterobacter sakazakii in powdered infant milk formula to gamma radiation.

    abstract:AIM:To evaluate the effect of starvation, heat, cold, acid, alkaline, chlorine and ethanol stresses on the resistance of Enterobacter sakazakii in powdered infant milk formula (PIMF) towards gamma radiation. METHODS AND RESULTS:Stressed cells of E. sakazakii ATCC 51329 and four other food isolate strains were mixed in...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02388.x

    authors: Osaili T,Al-Nabulsi A,Shaker R,Ayyash M,Olaimat A,Abu Al-Hasan A,Kadora K,Holley R

    更新日期:2008-08-01 00:00:00

  • Pseudomonas syringae pv. japonica (Mukoo 1955) Dye et al. 1980 is a junior synonym of Ps. syringae pv. syringae van Hall 1902.

    abstract::An investigation of the biochemical, nutritional and pathogenic reactions of strains of Pseudomonas syringae pv. japonica and Ps. syringae pv. syringae showed them to be indistinguishable. Pseudomonas syringae pv. japonica is a junior synonym of Ps. syringae pv. syringae. ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.1992.tb00744.x

    authors: Young JM

    更新日期:1992-10-01 00:00:00

  • Advantage of MALDI-TOF-MS over biochemical-based phenotyping for microbial identification illustrated on industrial applications.

    abstract:UNLABELLED:Fast microbial identification is becoming increasingly necessary in industry to improve microbial control and reduce biocide consumption. We compared the performances of two systems based on MALDI-TOF MS (VITEK MS and BIOTYPER) and two based on biochemical testing (BIOLOG, VITEK 2 Compact) with genetic metho...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12526

    authors: Urwyler SK,Glaubitz J

    更新日期:2016-02-01 00:00:00

  • Antibacterial effect of roselle extracts (Hibiscus sabadariffa), sodium hypochlorite and acetic acid against multidrug-resistant Salmonella strains isolated from tomatoes.

    abstract:UNLABELLED:Antibiotic-resistant Salmonella strains were isolated from saladette and red round type tomatoes, and an analysis done of the antibacterial activity of roselle calyx extracts against any of the identified strains. One hundred saladette tomato samples and 100 red round tomato samples were collected from publi...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12528

    authors: Gutiérrez-Alcántara EJ,Rangel-Vargas E,Gómez-Aldapa CA,Falfan-Cortes RN,Rodríguez-Marín ML,Godínez-Oviedo A,Cortes-López H,Castro-Rosas J

    更新日期:2016-02-01 00:00:00

  • Isolation of bacterial strains able to metabolize lignin and lignin-related compounds.

    abstract:UNLABELLED:In this study, we identified five strains isolated from soil and sediments able to degrade kraft lignin, aromatic dyes and lignin derivatives. Using 16S rRNA gene sequencing, the isolates were identified as Serratia sp. JHT01, Serratia liquefacien PT01, Pseudomonas chlororaphis PT02, Stenotrophomonas maltoph...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12581

    authors: Tian JH,Pourcher AM,Peu P

    更新日期:2016-07-01 00:00:00

  • Faecal contamination of echinoderms: first report of heavy Escherichia coli loading of sea urchins from a natural growing area.

    abstract:UNLABELLED:Although little evidence existed to support that view, European countries and in particular France, have regarded echinoderms, including sea urchins, as low risk in terms of feacal contamination. It is hypothesized that the sea urchins mode of feeding, which is based on grazing and differs from bivalve mollu...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12524

    authors: Bouchoucha M,Piquet JC,Chavanon F,Dufresne C,Le Guyader FS

    更新日期:2016-02-01 00:00:00

  • Isolation of Actinomyces viscosus strain GA: characteristics of its cellular biological retardant(s).

    abstract::Actinomyces viscosus strain GA produces an exocellular biological retardant(s) that prevents certain vegetable plants from becoming overgrown. The biological retardant(s) was assayed using the etiolated wheat coleoptile assay, and fractionation of culture supernatant fluid resulted in a partial purification of the ret...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1997.00178.x

    authors: Bryant FO,Cutler HG

    更新日期:1997-08-01 00:00:00

  • Biofilm contamination of high-touched surfaces in intensive care units: epidemiology and potential impacts.

    abstract::The aim of this study was to determine the epidemiology (location, microbial load, microbiome, presence/absence of biofilm and pathogens, including ESKAPE-Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter species, and antimicrobial susc...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13127

    authors: Costa DM,Johani K,Melo DS,Lopes LKO,Lopes Lima LKO,Tipple AFV,Hu H,Vickery K

    更新日期:2019-04-01 00:00:00

  • Bacterial diversity and community structure during fermentation of Chinese sauerkraut with Lactobacillus casei 11MZ-5-1 by Illumina Miseq sequencing.

    abstract::The bacterial diversity and community structure involved in Chinese sauerkraut is one of the most important factors shaping the final characteristics of traditional foods. In this research, Lactobacillus casei 11MZ-5-1 was applied in Chinese sauerkraut fermentation as a starter culture. Illumina Miseq sequencing analy...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12824

    authors: Du R,Ge J,Zhao D,Sun J,Ping W,Song G

    更新日期:2018-01-01 00:00:00

  • Effects of yeast probiotic formulation on viability, revival and protection against infection with Salmonella enterica ssp. enterica serovar Typhimurium in mice.

    abstract:AIMS:To compare the effects of five yeast probiotic formulations on viability, revival and washout kinetic in the digestive tract of mice, and the protection against an experimental infection with Salmonella enterica serovar Typhimurium. METHODS AND RESULTS:The number of viable cells in five commercial probiotic produ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2009.02732.x

    authors: Martins FS,Veloso LC,Arantes RM,Nicoli JR

    更新日期:2009-12-01 00:00:00

  • Pressure- vs. heat-induced bacterial stress in cooked poultry sausages: a preliminary study.

    abstract::Vacuum-packaged poultry cooked sausages were pressure-treated at 500 MPa by combinations of time (5-45 min) and temperature (2-80 degrees C) and later stored at 6-8 degrees C for 12 we. Mesophile and psychrotrophe counts were determined 1 d, 3, 6, 9 and 12 we after treatment and compared with those of cooked sausages ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00611.x

    authors: Yuste J,Mor-Mur M,Capellas M,Pla R

    更新日期:1999-10-01 00:00:00

  • Yeast as an expression system for producing virus-like particles: what factors do we need to consider?

    abstract::The development of various types of virus-like particles (VLPs) has accelerated over the past two decades as the importance of VLPs for generating next-generation vaccines has been appreciated. Yeast has advantages such as scalable fermentation, low risk of contamination by adventitious agents, low production costs an...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/lam.12695

    authors: Kim HJ,Kim HJ

    更新日期:2017-02-01 00:00:00

  • Isolation and genome analysis of a lytic Pasteurella multocida Bacteriophage PMP-GAD-IND.

    abstract::Currently used alum precipitated and oil adjuvant vaccines against HS caused by Pasteurella multocida B:2, have side effects and short-lived immunity, leading to regular catastrophic outbreaks in bovines in Asian subcontinent. The need for the development of an improved vaccine with longer immunity and the ability to ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13010

    authors: Qureshi S,Saxena HM,Imam N,Kashoo Z,Sharief Banday M,Alam A,Malik MZ,Ishrat R,Bhat B

    更新日期:2018-09-01 00:00:00

  • Recent strategies for efficient production of polyhydroxyalkanoates by micro-organisms.

    abstract::Polyhydroxyalkanoates (PHAs) are polyesters accumulated by many bacteria under unbalanced growth conditions, and have been used to meet the various demands in areas of agriculture, medicine, and materials especially belong to a rapidly expanding area of biomedical research. Unfortunately, the high production cost than...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12511

    authors: Liu CC,Zhang LL,An J,Chen B,Yang H

    更新日期:2016-01-01 00:00:00

  • Production of red pigment by submerged culture of Paecilomyces sinclairii.

    abstract:AIMS:From a survey of submerged culture of edible mushrooms, a high pigment-producing fungus Paecilomyces sinclairii was selected and its optimal culture conditions investigated. METHODS AND RESULTS:The optimal culture conditions for pigment production were as follows: inoculum age, 3 d; temperature, 25 degrees C; ini...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01168.x

    authors: Cho YJ,Park JP,Hwang HJ,Kim SW,Choi JW,Yun JW

    更新日期:2002-01-01 00:00:00

  • Hydrolytic properties of crude alpha-L-rhamnosidases produced by several wild strains of mesophilic fungi.

    abstract:AIMS:Observation of the dependence of alpha-L-rhamnosidase activity on pH and temperature and the capability to hydrolyse concentrated naringin solutions and hesperidin suspensions of enzyme complexes produced by several fungi. METHODS AND RESULTS:The enzymes were produced by several wild strains of mesophilic fungi g...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01115.x

    authors: Scaroni E,Cuevas C,Carrillo L,Ellenrieder G

    更新日期:2002-01-01 00:00:00

  • Lactobacilli prevent hydroxy radical production and inhibit Escherichia coli and Enterococcus growth in system mimicking colon fermentation.

    abstract:AIMS:To explore the effect of Lactobacillus on redox state of colon chyme. METHODS AND RESULTS:Nine Lactobacillus strains were studied for the inhibition of lipid peroxide formation in Fe(2+)/ascorbate system and for their ability to chelate 'free' ferrous ion. The result shows both properties were strain specific and...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2009.02786.x

    authors: Sun J,Hu XL,Le GW,Shi YH

    更新日期:2010-03-01 00:00:00