High-level secretion of human bikunin from recombinant Pichia pastoris.

abstract：AIMS:The study was carried out to evaluate the use of randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) as a method for the identification of lactobacilli isolated from meat products. METHODS AND RESULTS:RAPD-PCR with primers M13 and D8635 was applied to the identification and intraspecific diffe...

journal_title：Letters in applied microbiology

authors： Andrighetto C,Zampese L,Lombardi A

更新日期：2001-07-01 00:00:00

abstract：AIMS:To investigate the incidence of Campylobacter and Salmonella contamination associated with supermarket and butchers' shop chicken and related packaging. METHOD AND RESULTS:Three hundred raw samples (whole chicken, chicken breast with skin or chicken pieces) were purchased on a monthly basis for seven months. Pack...

journal_title：Letters in applied microbiology

authors： Harrison WA,Griffith CJ,Tennant D,Peters AC

更新日期：2001-12-01 00:00:00

abstract：AIMS:To test a performance of the microbiological safety cabinets (MSCs) according to the type of MSCs in microbial laboratories. METHODS AND RESULTS:Tests were carried out to assess the performance of 31 MSCs in 14 different facilities, including six different biological test laboratories in six hospitals and eight d...

journal_title：Letters in applied microbiology

authors： Hwang SH,Yi TW,Cho KH,Lee IM,Yoon CS

更新日期：2011-09-01 00:00:00

abstract：:The use of energy dispersive analysis of X-rays (EDAX) to identify and quantify the presence of chlorhexidine diacetate (CHA) within Saccharomyces cerevisiae cells was examined. Chlorine was used as the elemental marker tag. Saccharomyces cerevisiae cells exposed to 1000 micrograms ml-1 CHA took up increasing amounts ...

journal_title：Letters in applied microbiology

authors： Hiom SJ,Hann AC,Furr JR,Russell AD

更新日期：1995-06-01 00:00:00

abstract：AIMS:Observation of the dependence of alpha-L-rhamnosidase activity on pH and temperature and the capability to hydrolyse concentrated naringin solutions and hesperidin suspensions of enzyme complexes produced by several fungi. METHODS AND RESULTS:The enzymes were produced by several wild strains of mesophilic fungi g...

journal_title：Letters in applied microbiology

authors： Scaroni E,Cuevas C,Carrillo L,Ellenrieder G

更新日期：2002-01-01 00:00:00

abstract：AIMS:This study evaluated the inactivation of virulent Yersinia pestis dried on polymers, steel, and glass surfaces using vapour-phase hydrogen peroxide. METHODS AND RESULTS:A suspension of Y. pestis CO92 (1.70 x 10(8) CFU) was dried on 10 different types of test surfaces and exposed to vapour-phase hydrogen peroxide ...

journal_title：Letters in applied microbiology

authors： Rogers JV,Richter WR,Shaw MQ,Choi YW

更新日期：2008-10-01 00:00:00

abstract：AIMS:  To show that application of copper hydroxide citrus sprays mixed with field source water (possibly contaminated) will not support Escherichia coli on plant surfaces. Environmental stresses of transient phyllosphere bacteria and presence of copper will eradicate these bacteria before harvest. METHODS AND RESULTS...

journal_title：Letters in applied microbiology

authors： Narciso JA,Ference CM,Ritenour MA,Widmer WW

更新日期：2012-02-01 00:00:00

abstract：AIMS: Lesions of DNA are removed by nucleotide excision repair (NER) process in the living systems. NER process-related host factors are believed to aid recovery steps during viral integration. Here, we report identification and characterization of a DNA repair molecule Rad23 from kuruma shrimp Marsupenaeus japonicus. ...

journal_title：Letters in applied microbiology

authors： Sudhakaran R,Okugawa S,Mekata T,Inada M,Yoshimine M,Nishi J,Ozono C,Kono T,Sakai M,Itami T

更新日期：2011-07-01 00:00:00

abstract：AIMS:To evaluate mycobiota and aflatoxins B(1) (AFB(1)), B(2) (AFB(2)), G(1) (AFG(1)), G(2) (AFG(2)) and fumonisin B(1) (FB(1)) contamination in different malted barley types and brands and brewer's grain collected from a major Argentinean brewery. METHODS AND RESULTS:Total fungal counts were performed using the plate...

journal_title：Letters in applied microbiology

authors： Gonzalez Pereyra ML,Rosa CA,Dalcero AM,Cavaglieri LR

更新日期：2011-12-01 00:00:00

abstract：AIMS:To survey for hepatitis A virus (HAV) and hepatitis E virus (HEV) contamination in edible bivalve shellfish. METHODS AND RESULTS:A total of 213 shellfish (52 oysters, 69 cockles and 92 mussels) collected from a culture farm and two retailed markets were investigated for HAV and HEV contamination by reverse transc...

journal_title：Letters in applied microbiology

authors： Namsai A,Louisirirotchanakul S,Wongchinda N,Siripanyaphinyo U,Virulhakul P,Puthavathana P,Myint KS,Gannarong M,Ittapong R

更新日期：2011-12-01 00:00:00

abstract：AIMS:This work was carried out to determine if there was a difference in the microbial population of the rumen associated with daylength at which sheep are housed. METHODS AND RESULTS:Denaturing gradient gel electrophoresis (DGGE) was used to study the ciliate and bacterial diversity in the rumen of Soay rams kept in ...

journal_title：Letters in applied microbiology

authors： McEwan NR,Abecia L,Regensbogenova M,Adam CL,Findlay PA,Newbold CJ

更新日期：2005-01-01 00:00:00

abstract：AIMS:This study was performed to investigate the microbiological contamination of digestate product (DP) obtained from the anaerobic co-digestion of bovine manure and agricultural by-products. METHODS AND RESULTS:Microbiological analyses were performed on bovine manure, fresh DP, liquid and solid fractions and stored ...

journal_title：Letters in applied microbiology

authors： Bonetta S,Ferretti E,Bonetta S,Fezia G,Carraro E

更新日期：2011-11-01 00:00:00

abstract：:A strain named as Pseudomonas aeruginosa 2016NX1, which could produce phenazine and cereusitin, was isolated from the root of Millettia specisoa. Phenazines were extracted, isolated and purified by chloroform, thin-layer chromatography, column chromatography and high-performance liquid chromatography. Then the purifie...

journal_title：Letters in applied microbiology

authors： Liu TT,Ye FC,Pang CP,Yong TQ,Tang WD,Xiao J,Shang CH,Lu ZJ

更新日期：2020-09-01 00:00:00

abstract：AIMS:The objective of this study is to actively express a novel fibrinolytic enzyme, subtilisin DFE (douchi fibrinolytic enzyme), in Escherichia coli. METHODS AND RESULTS:The DNA fragments encoding pro-subtilisin DFE was amplified and cloned into the vector pET32a to obtain N-terminal Trx fusion expression plasmid. Th...

journal_title：Letters in applied microbiology

authors： Zhang RH,Xiao L,Peng Y,Wang HY,Bai F,Zhang YZ

更新日期：2005-01-01 00:00:00

abstract：AIM:The aim of this work was to evaluate the effect of Planococcus ficus infection in red wine grapes on Aspergillus section Nigri and ochratoxin A (OTA) contamination. METHODS AND RESULTS:During 2006/2007 and 2008/2009 vintages, Merlot, Malbec and Cabernet Sauvignon varieties divided into two categories of grape samp...

journal_title：Letters in applied microbiology

authors： Chiotta ML,Ponsone ML,Torres AM,Combina M,Chulze SN

更新日期：2010-08-01 00:00:00

abstract：UNLABELLED:Most of the current research into the quantification of soil-borne pathogenic oomycetes lacks determination of DNA extraction efficiency, probably leading to an incorrect estimation of DNA quantity. In this study, we developed a convenient method by using a 100 bp artificially synthesized DNA sequence derive...

journal_title：Letters in applied microbiology

authors： Li M,Ishiguro Y,Kageyama K,Zhu Z

更新日期：2015-08-01 00:00:00

abstract：:The Andrias davidianus has been known as a traditional Chinese medicine for a long time. Its blood is considered as a waste or by-product of the meat production industry. Although there are reports on isolation of the antimicrobial peptides from different resources, there are no reports of their isolation from A. davi...

journal_title：Letters in applied microbiology

authors： Pei J,Feng Z,Ren T,Sun H,Han H,Jin W,Dang J,Tao Y

更新日期：2018-01-01 00:00:00

abstract：:Yellow wine lees (YWL) are the main co-products in yellow wine industry with unbalanced amino acid (AA) profiles. Solid-state fermentation was employed in this study to upgrade the YWL for ruminant animals. A 3 × 3 orthogonal design was conducted to optimize the fermentation condition for optimal crude protein (CP) yi...

journal_title：Letters in applied microbiology

authors： Yao KY,Zhang TZ,Wang HF,Liu JX

更新日期：2018-12-01 00:00:00

abstract：:Plant proteases play a fundamental role in several processes like growth, development and in response to biotic and abiotic stress. In particular, aspartic proteases (AP) are expressed in different plant organs and have antimicrobial activity. Previously, we purified an AP from Salpichroa origanifolia fruits called sa...

journal_title：Letters in applied microbiology

authors： Díaz ME,Rocha GF,Kise F,Rosso AM,Guevara MG,Parisi MG

更新日期：2018-08-01 00:00:00

abstract：AIMS:Aims of investigation: (i) develop a quantitative RT-PCR for noroviruses and (ii) evaluate it on environmental samples. METHODS AND RESULTS:Noroviruses in environmental water samples were concentrated by adsorption/elution/flocculation. Sewage was processed by clarification and protein flocculation. Norovirus-spe...

journal_title：Letters in applied microbiology

authors： Laverick MA,Wyn-Jones AP,Carter MJ

更新日期：2004-01-01 00:00:00

abstract：:Three groups of substances were identified which inhibit the bactericidal activity of bacitracin. Beside two divalent metals, Mg2+ and Ca2+, and a metabolite with chelating properties, citrate, the most pronounced effect was observed with pyrophosphate. Metals probably prevent access of bacitracin to the lipid carrier...

journal_title：Letters in applied microbiology

authors： Podlesek Z,Comino A

更新日期：1994-08-01 00:00:00

abstract：AIMS:To identify and characterize amino acid transport in Leuconostoc mesenteroides. METHODS AND RESULTS:The transport of labelled amino acids was measured in whole cells of Leuc. mesenteroides CNRZ 1273. Systems were operative under physiological conditions of growth, energy dependent and differed from peptide transp...

journal_title：Letters in applied microbiology

authors： Gendrot F,Foucaud-Scheunemann C,Ferchichi M,Hemme D

更新日期：2002-01-01 00:00:00

abstract：:During investigation of a gastroenteritis outbreak in a chronic care institution, Norwalk virus was found in stool specimens from two individuals and bacterial isolates presumptively identified as Bacillus cereus were isolated from four individuals (including one with Norwalk virus) and spice. Phage typing confirmed a...

journal_title：Letters in applied microbiology

authors： Jackson SG,Goodbrand RB,Ahmed R,Kasatiya S

更新日期：1995-08-01 00:00:00

abstract：:Parabens have been widely used as antimicrobial preservatives in cosmetics, pharmaceuticals, foods and beverages. Commonly, methyl-, ethyl-, propyl- and butylparaben are used independently or in combination to maintain the quality of industrial products, and they are considered to have low toxicity. However, recent ev...

journal_title：Letters in applied microbiology

authors： Murata W,Yamaguchi Y,Fujita KI,Yamauchi K,Tanaka T,Ogita A

更新日期：2019-12-01 00:00:00

abstract：AIMS:This work aims to investigate the binding capability of certain domains at N terminus of the protein NP_785232 of Lactobacillus plantarum to Caco-2 cells and to test the usage of affinity chromatography to isolate the human mucus component that interacts with them. METHODS AND RESULTS:Recombinant proteins contain...

journal_title：Letters in applied microbiology

authors： Du L,Liu F,Ju X,Huo G

更新日期：2010-10-01 00:00:00

abstract：:Blown pack spoilage (BPS) of vacuum packaged beef is caused by psychrotolerant and psychrophilic Clostridium species, primarily Clostridium estertheticum and Clostridium gasigenes. The aim of this study was to investigate the environmental niches and impact of season on these BPS Clostridium spp. on Irish beef farms. ...

journal_title：Letters in applied microbiology

authors： Esteves E,Whyte P,Gupta TB,Bolton DJ

更新日期：2020-12-01 00:00:00

abstract：AIMS:The risk of hepatitis C virus infection in hospital environments can be assessed not only by studying epidemiological data and work practices, but also by the detection of these viruses (or indicators thereof) in health-care settings, on instruments etc. METHODS:Since standardized techniques specific to this end ...

journal_title：Letters in applied microbiology

authors： Carducci A,Verani M,Casini B,Giuntini A,Mazzoni F,Rovini E,Passaglia A,Giusti L,Valenza A,Lombardi R

更新日期：2002-01-01 00:00:00

abstract：:Candida species are increasingly important fungal pathogens. The reaction of rat alveolar macrophages (AM) to Candida albicans was compared with that to C. glabrata and C. krusei. Phagocytosis of C. glabrata was similar to that of C. albicans, but significantly slower for C. krusei due to reduced attachment. After ops...

journal_title：Letters in applied microbiology

authors： Nessa K,Johansson A,Jarstrand C,Camner P

更新日期：1997-09-01 00:00:00

abstract：AIMS:To investigate the diversity and dissemination of tetracycline resistance genes in isolates from estuarine waters. METHODS AND RESULTS:Forty-two out of 164 multi-resistant isolates previously obtained were resistant or less-susceptible to tetracycline, as evaluated by the disc diffusion method. Minimal inhibitory...

journal_title：Letters in applied microbiology

authors： Henriques IS,Fonseca F,Alves A,Saavedra MJ,Correia A

更新日期：2008-12-01 00:00:00

abstract：AIMS:To isolate a bacterium capable of degrading 3 beta-acetoxypregna-5,16-diene-20-one (16-DPA) to androsta-1,4-diene-3,17-dione (ADD) and to decipher the biodegradation pathway. METHODS AND RESULTS:Isolation on mineral salt agar containing 16-DPA as sole carbon source yielded two bacteria identified as Pseudomonas d...

journal_title：Letters in applied microbiology

authors： Patil S,Shukla A,Shinde K,Banerjee T

更新日期：2002-01-01 00:00:00