X-ray microanalysis of chlorhexidine-treated cells of Saccharomyces cerevisiae.

abstract：:Polymerase chain reaction (PCR) was used after a short pre-enrichment culture to detect Salmonella subspecies in chicken fillets. A direct PCR assay performed with chicken meat inoculated with Salmonella Typhimurium produced no PCR products. Six different DNA extraction protocols were tested to recover efficiently Sal...

journal_title：Letters in applied microbiology

authors： Soumet C,Ermel G,Fach P,Colin P

更新日期：1994-11-01 00:00:00

abstract：AIMS: Lesions of DNA are removed by nucleotide excision repair (NER) process in the living systems. NER process-related host factors are believed to aid recovery steps during viral integration. Here, we report identification and characterization of a DNA repair molecule Rad23 from kuruma shrimp Marsupenaeus japonicus. ...

journal_title：Letters in applied microbiology

authors： Sudhakaran R,Okugawa S,Mekata T,Inada M,Yoshimine M,Nishi J,Ozono C,Kono T,Sakai M,Itami T

更新日期：2011-07-01 00:00:00

abstract：:Synthesis of gold nanoparticles (AuNPs) has become a needed domain of applied science. Biological method for synthesis of AuNPs by Trichoderma hamatum SU136 aqueous mycelial extract was achieved. The culture filtrate of the fungus was exposed to three different concentrations of gold chloride. The culture filtrate of ...

journal_title：Letters in applied microbiology

authors： Abdel-Kareem MM,Zohri AA

更新日期：2018-11-01 00:00:00

abstract：:A strain of Bacillus thuringiensis, STB-1, toxic against Spodoptera exigua, was isolated. Bacillus thuringiensis STB-1 produced bipyramidal inclusions and reacted with the H antiserum of B. thuringiensis ssp. kurstaki. The plasmid and protein profiles of B. thuringiensis STB-1 were compared with those of its reference...

journal_title：Letters in applied microbiology

authors： Chang JH,Roh JY,Je YH,Park HW,Jin BR,Woo SD,Kang SK

更新日期：1998-05-01 00:00:00

abstract：AIMS:This study assessed the plant growth-promoting ability of the bacterial strains Ochrobactrum intermedium (isolate CrT-1) and Bacillus cereus (isolate S-6). METHODS AND RESULTS:Two chromium resistant bacterial strains isolated from chromium-contaminated wastewater and soils were identified as O. intermedium CrT-1 ...

journal_title：Letters in applied microbiology

authors： Faisal M,Hasnain S

更新日期：2006-10-01 00:00:00

abstract：AIMS:Aims of investigation: (i) develop a quantitative RT-PCR for noroviruses and (ii) evaluate it on environmental samples. METHODS AND RESULTS:Noroviruses in environmental water samples were concentrated by adsorption/elution/flocculation. Sewage was processed by clarification and protein flocculation. Norovirus-spe...

journal_title：Letters in applied microbiology

authors： Laverick MA,Wyn-Jones AP,Carter MJ

更新日期：2004-01-01 00:00:00

abstract：:Random amplification of polymorphic DNA (RAPD) is proving to be a useful technique in studying the epidemiology of micro-organisms. The technique can be troublesome and time consuming to establish due to the essentially empirical approach to optimization. By standardization of certain parameters and use of a commercia...

journal_title：Letters in applied microbiology

authors： Hilton AC,Banks JG,Penn CW

更新日期：1997-04-01 00:00:00

abstract：UNLABELLED:Escherichia coli is part of the normal microflora of the intestines of mammals. However, among the enteric pathogens, it is one of the leading causes of intestinal diseases, especially Shiga toxigenic E. coli, which can cause diarrhoea, haemorrhagic colitis and complications like haemolytic uraemic syndrome ...

journal_title：Letters in applied microbiology

authors： Ribeiro LF,Barbosa MM,de Rezende Pinto F,Guariz CS,Maluta RP,Rossi JR,Rossi GA,Lemos MV,do Amaral LA

更新日期：2016-03-01 00:00:00

abstract：AIMS:The objective of this study is to actively express a novel fibrinolytic enzyme, subtilisin DFE (douchi fibrinolytic enzyme), in Escherichia coli. METHODS AND RESULTS:The DNA fragments encoding pro-subtilisin DFE was amplified and cloned into the vector pET32a to obtain N-terminal Trx fusion expression plasmid. Th...

journal_title：Letters in applied microbiology

authors： Zhang RH,Xiao L,Peng Y,Wang HY,Bai F,Zhang YZ

更新日期：2005-01-01 00:00:00

abstract：:The effect of liquid mechanical vibration on the adhesion of Saccharomyces cerevisiae cells to the internal glass surface of a pipette was studied using a 25 Hz vibration source. The maximum vibration amplitude was 1.06 mm (peak to peak) along the pipette direction. Relative movements between the pipette and yeast sus...

journal_title：Letters in applied microbiology

authors： Jiang C,Yu H,Ding Z,Ma Y

更新日期：1996-07-01 00:00:00

abstract：AIMS:To investigate if one hypothetical protein from Chlamydophila pneumoniae AR39 exerts UbiG-like function by complementary experiments. METHODS AND RESULTS:Proteins UbiG have a signature S-adenosylmethionine-binding motif compared with other methyltransferases. Probing with the conserved motif, one hypothetical pro...

journal_title：Letters in applied microbiology

authors： Liu J,Zhang X,Liu J

更新日期：2007-07-01 00:00:00

abstract：:For pyruvate-producing strains, intracellular reduced nicotinamide adenine dinucleotide (NADH) accumulation is the main reason for the glycolysis inhibition. Comparing with glucose, using sodium gluconate as carbon source brought a decrease in NADH production and an increase in pyruvate production in engineered strain...

journal_title：Letters in applied microbiology

authors： Yang M,Mu T,Zhong W,Olajuyin AM,Xing J

更新日期：2017-08-01 00:00:00

abstract：:The use of buoyant density centrifugation (BDC) to prepare samples for PCR analysis of food pathogens is described. Blue cheese and milk homogenates were inoculated with Shigella flexneri and layered on top of Percoll media. After BDC, the food homogenates remained in the upper part of the centrifuge tube, separated f...

journal_title：Letters in applied microbiology

authors： Lindqvist R,Norling B,Lambertz ST

更新日期：1997-04-01 00:00:00

abstract：UNLABELLED:The unicellular freshwater green microalga Haematococcus pluvialis is the richest source of natural astaxanthin. Since accumulation of astaxanthin differs significantly among various algal strains at different stages, it is therefore critical to develop an effective high-throughput assay for rapid screening ...

journal_title：Letters in applied microbiology

authors： Liu JH,Song L,Huang Q

更新日期：2016-02-01 00:00:00

abstract：AIMS:Aim of the study was to develop a medium for optimal heparinase production with a strain of Aspergillus flavus (MTCC-8654) by using a multidimensional statistical approach. METHODS AND RESULTS:Statistical optimization of intracellular heparinase production by A. flavus, a new isolate, was investigated. Plackett-B...

journal_title：Letters in applied microbiology

authors： Banga J,Tripathi CK

更新日期：2009-08-01 00:00:00

abstract：AIMS:Evaluation of the activity profiles of standard prokaryotic translation inhibitors with different physicochemical properties under whole-cell and cell-free conditions. METHODS AND RESULTS:The minimal inhibitory concentration values (cell-free/whole-cell microg ml(-1)) for three aminoglycosides (neomycin, 0.01/6.9...

journal_title：Letters in applied microbiology

authors： Weidlich M,Klammt C,Bernhard F,Karas M,Stein T

更新日期：2008-02-01 00:00:00

abstract：AIM:To stimulate poly-beta-hydroxybutyrate (PHB) accumulation in Synechocystis sp. PCC 6803 by manipulating culture conditions. METHODS AND RESULTS:Stationary phase cultures of Synechocystis sp. PCC 6803 were subjected to N- and P-deficiency, chemoheterotrophy and limitations of gas-exchange. Enhanced PHB accumulation...

journal_title：Letters in applied microbiology

authors： Panda B,Mallick N

更新日期：2007-02-01 00:00:00

abstract：:A method for the diagnosis of mycobacterial infections by PCR amplification followed by selective restriction enzyme digestion of the PCR product was developed. The amplified DNA sequence used in this study occurs within the gene encoding for the mycobacterial 65 kDa heat shock protein (Hance et al. 1989), which is fo...

journal_title：Letters in applied microbiology

authors： Rodrigo G,Källenius G,Hoffmann E,Svenson SB

更新日期：1992-08-01 00:00:00

abstract：:Reagent-derived contamination can compromise the integrity of microbiome data, particularly in low microbial biomass samples. This contamination has recently been attributed to the 'kitome' (contamination introduced by the DNA extraction kit), prior to which attention was mostly paid to potential contamination introdu...

journal_title：Letters in applied microbiology

authors： Stinson LF,Keelan JA,Payne MS

更新日期：2019-01-01 00:00:00

abstract：AIMS:Species-specific PCR was applied to identify Lactobacillus brevis and the sensitivity and the specificity of the protocol were determined. METHODS AND RESULTS:Strains of Lact. brevis obtained from foods, particularly dairy products, and various strain collections, were identified by PCR using primers which amplif...

journal_title：Letters in applied microbiology

authors： Guarneri T,Rossetti L,Giraffa G

更新日期：2001-11-01 00:00:00

abstract：:In order to evaluate possible indicators of viral aerosol contamination in sewage treatment plants, a year-long study was carried out on the relationships between the presence of cytopathogenic viruses and the counts of total bacteria, faecal streptococci and somatic coliphages in samples collected at various distance...

journal_title：Letters in applied microbiology

authors： Carducci A,Gemelli C,Cantiani L,Casini B,Rovini E

更新日期：1999-03-01 00:00:00

abstract：AIMS:To isolate benzene-degrading strains from neutral and alkaline groundwaters contaminated by benzene, toluene, ethylbenzene, xylenes (BTEX) from the SIReN aquifer, UK, and to test their effective pH range and ability to degrade TEX. METHODS AND RESULTS:The 14 isolates studied had an optimum pH for growth of 8, and...

journal_title：Letters in applied microbiology

authors： Fahy A,Ball AS,Lethbridge G,Timmis KN,McGenity TJ

更新日期：2008-07-01 00:00:00

abstract：AIMS:To develop an intergeneric conjugation system for rimocidin-producing Streptomyces rimosus. METHODS AND RESULTS:High efficiencies of conjugation [10(-2)-10(-3) transconjugants/recipient colony forming units (CFU)] were obtained when spores of S. rimosus were heat treated at 40 degrees C for 10 min prior to mixing...

journal_title：Letters in applied microbiology

authors： Phornphisutthimas S,Sudtachat N,Bunyoo C,Chotewutmontri P,Panijpan B,Thamchaipenet A

更新日期：2010-05-01 00:00:00

abstract：UNLABELLED:We investigated the role of the Bradyrhizobium japonicum lpcC gene, encoding a mannosyl transferase, involved in the lipopolysaccharide (LPS) biosynthesis. The inactivation of the lpcC gene considerably altered the LPS structure and the cell surface properties. LPS analysis showed that the lpcC mutant JS715 ...

journal_title：Letters in applied microbiology

authors： Lee HI,In YH,Jeong SY,Jeon JM,Noh JG,So JS,Chang WS

更新日期：2014-07-01 00:00:00

abstract：AIMS:Oenococcus oeni is a lactic acid bacterium used in wine fermentation. Two open reading frames (orfB and orfC) were identified in the upstream region of the hsp18 gene, encoding the small heat-shock protein Lo18. Expression of these genes in conditions of acid stress was studied in Escherichia coli. METHODS AND RE...

journal_title：Letters in applied microbiology

authors： Morel F,Delmas F,Jobin MP,Diviès C,Guzzo J

更新日期：2001-08-01 00:00:00

abstract：:Enterococci were isolated from faecal droppings of chickens in broiler and layer farms and the susceptibilities to nine therapeutic antimicrobial agents and six growth-promoting antibiotics were determined by the agar dilution method. Resistance to therapeutic antimicrobial agents such as ampicillin, clindamycin, eryt...

journal_title：Letters in applied microbiology

authors： Yoshimura H,Ishimaru M,Endoh YS,Kojima A

更新日期：2000-12-01 00:00:00

abstract：AIMS:An agar medium containing a range of related chlorophenoxyalkanoic acid herbicides, 2,4-dichlorophenoxyacetic acid (2,4-D), 2-methyl-4-chlorophenoxyacetic acid (MCPA), racemic mecoprop, (R)-mecoprop and racemic 2,4-DP (2-(2,4-dichlorophenoxy) propionic acid) was developed to assess the catabolic activity of a rang...

journal_title：Letters in applied microbiology

authors： Smejkal CW,Vallaeys T,Burton SK,Lappin-Scott HM

更新日期：2001-04-01 00:00:00

abstract：:The novel Eriocheir sinensis reovirus (EsRV) is a pathogen that causes severe disease and high mortality rates in cultivated crabs. Here, we established a highly sensitive and specific rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that was cheaper and more suitable for field applic...

journal_title：Letters in applied microbiology

authors： Ma Y,Dai T,Serwadda A,Shen H

更新日期：2016-11-01 00:00:00

abstract：AIMS:To test the efficacy of Lactobacillus johnsonii FI9785 in reducing the colonization and shedding of Salmonella enterica serotype Enteritidis, Escherichia coli O78:K80 and Clostridium perfringens in poultry. METHODS AND RESULTS:Specific pathogen-free chicks (1 day old) were dosed with a single oral inoculum of 1x1...

journal_title：Letters in applied microbiology

authors： La Ragione RM,Narbad A,Gasson MJ,Woodward MJ

更新日期：2004-01-01 00:00:00

abstract：UNLABELLED:This study reports the development of a loop-mediated isothermal amplification (LAMP) reaction for the detection of Pythium myriotylum. The primer set targeting the ITS sequence of P. myriotylum worked most efficiently at 60°C and allowed the detection of P. myriotylum DNA within 30 min by fluorescence monit...

journal_title：Letters in applied microbiology

authors： Fukuta S,Takahashi R,Kuroyanagi S,Ishiguro Y,Miyake N,Nagai H,Suzuki H,Tsuji T,Hashizume F,Watanabe H,Kageyama K

更新日期：2014-07-01 00:00:00