Abstract:
:Parabens have been widely used as antimicrobial preservatives in cosmetics, pharmaceuticals, foods and beverages. Commonly, methyl-, ethyl-, propyl- and butylparaben are used independently or in combination to maintain the quality of industrial products, and they are considered to have low toxicity. However, recent evidence has suggested that parabens are toxic in mammalian cells, and parabens have been associated with allergic-contact dermatitis, breast cancer and changes in testosterone levels. Sulforaphane, a cruciferous vegetable-derived isothiocyanate, was effective in decreasing the growth inhibitory concentrations of ethyl-, propyl-, butyl- and methylparaben in the yeast Saccharomyces cerevisiae. The sulforaphane-enhanced fungicidal effects of methylparaben were deemed to be caused by drastic cell membrane damage and the leakage of internal substances, such as nucleotides, from S. cerevisiae cells. Moreover sulforaphane markedly decreased the minimum concentration of methyl- and ethylparaben required to inhibit the growth of various microbes, such as the pathogenic yeast that causes severe mycosis, Candida albicans; the filamentous fungi Aspergillus niger; and the Gram-negative bacterium Escherichia coli. Enhanced antimicrobial activity from the beneficial components of edible plants may increase paraben efficacy at low concentrations and minimize preservative-induced side effects in consumers. SIGNIFICANCE AND IMPACT OF THE STUDY: Sulforaphane, a natural and beneficial cruciferous vegetable-derived isothiocyanate, increased the ability of parabens to disrupt fungal cell membranes. Paraben-containing products have been reported to cause allergic contact dermatitis and drug hypersensitivity; therefore, methods to preserve organic products that may reduce the concentrations of parabens are both timely and necessary. In this study, we found that the combined antimicrobial effects of sulforaphane and parabens had the potential to reduce the paraben concentration needed to preserve organic products, thereby indicating that paraben toxicity may be reduced without affecting its activity as a preservative.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Murata W,Yamaguchi Y,Fujita KI,Yamauchi K,Tanaka T,Ogita Adoi
10.1111/lam.13230subject
Has Abstractpub_date
2019-12-01 00:00:00pages
403-410issue
6eissn
0266-8254issn
1472-765Xjournal_volume
69pub_type
杂志文章abstract:AIMS:To examine the ability of Agrobacterium to attach to Metarhizium anisopliae var. acridum strain CG423 under co-cultivation and to develop an Agrobacterium-mediated method of gene delivery into strain CG423, a promising agent for biological control of grasshoppers. METHODS AND RESULTS:The co-cultivation of Agrobac...
journal_title:Letters in applied microbiology
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doi:10.1111/j.1472-765X.2006.02092.x
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doi:10.1111/j.1472-765x.2003.01452.x
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abstract:AIMS:We report the analysis of genetic determinants conferring resistance to pristinamycin in Staphylococcus epidermidis strains and epidemiology typing of these strains by pulsed-field gel electrophoresis. METHODS AND RESULTS:Staphylococcus epidermidis (346 isolates) were searched for strains with pristinamycin resis...
journal_title:Letters in applied microbiology
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doi:10.1111/j.1472-765X.2007.02318.x
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abstract:UNLABELLED:Human bikunin is a glycoprotein that exhibits trypsin inhibitory activity against serine proteases, and is effective in clinic. However, limited productivity and high price of human bikunin retard its further application. In this study, a high-yield, low-cost process of recombinant human bikunin (rh-bikunin)...
journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02258.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1472-765X.2008.02490.x
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abstract:AIMS:Enzyme kinetics of purified laccases from six different Pleurotus ostreatus strains were determined in the oxidation of syringaldazine, guaiacol and ABTS. METHODS AND RESULTS:Significant differences in the kinetic constants were found. Catalytic activity (kcat) ranged from 19 to 941 U mg(-1) for syringaldazine, f...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.00913.x
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abstract::Enterohaemorrhagic Escherichia coli (EHEC) agar was evaluated for its ability to recover one isolate of each of three serotypes (O157:H7, O26 and O113:H21) of shiga toxin-producing E. coli (STEC) from raw mince, pasteurized milk and salami after enrichment. The method detected around one colony-forming unit (cfu) in 2...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2000.00676.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01066.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.02027.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01407.x
更新日期:2003-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12871
更新日期:2018-05-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12670
更新日期:2017-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1997.00341.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1997.t01-1-00069.x
更新日期:1997-07-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01948.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.01005.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01994.x
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journal_title:Letters in applied microbiology
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doi:10.1046/j.1472-765x.2002.01098.x
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
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更新日期:2020-11-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02132.x
更新日期:2007-06-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1472-765X.2006.01945.x
更新日期:2006-06-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1993.tb01401.x
更新日期:1993-04-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1995.tb00438.x
更新日期:1995-04-01 00:00:00
abstract:AIM:To estimate the prevalence of thermotolerant Campylobacter spp. in commercially reared partridges (Perdix perdix) in southern Italy. METHODS AND RESULTS:Cloacal swabs of partridges (n = 240), equally distributed between male and female birds, from a game bird farm located in the Southern Italy were examined for th...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02667.x
更新日期:2009-09-01 00:00:00
abstract::Colicin V is a small, proteinaceous bacterial toxin, produced by many strains of Escherichia coli and other members of the Enterobacteriaceae, that fits the definition of class II bacteriocins of Gram-positive bacteria. Export of colicin V is dependent on specific ABC (ATP-binding cassette) secretion proteins which re...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00571.x
更新日期:1999-07-01 00:00:00
abstract:AIMS:To compare three methods for DNA extraction from Mycobacterium bovis, Mycobacterium tuberculosis and Mycobacterium avium subsp. avium. METHODS AND RESULTS:The DNA was extracted from mycobacterial cultures using enzymatic extraction, combined bead beating and enzymatic extraction and cetyltrimethylammonium bromide...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2008.02372.x
更新日期:2008-07-01 00:00:00
abstract:AIMS:In this study, a capillary polymerase chain reaction (cPCR) was applied for Salmonella detection from poultry meat. METHODS AND RESULTS:Salmonella detection limits of the optimized cPCR were determined with DNA templates from the samples of tetrathionate broth (TTB), Rappaport Vassiliadis broth (RVB) and selenite...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.02033.x
更新日期:2007-01-01 00:00:00