Abstract:
AIMS:To compare three methods for DNA extraction from Mycobacterium bovis, Mycobacterium tuberculosis and Mycobacterium avium subsp. avium. METHODS AND RESULTS:The DNA was extracted from mycobacterial cultures using enzymatic extraction, combined bead beating and enzymatic extraction and cetyltrimethylammonium bromide (CTAB) extraction. The yield and quality of DNA were compared by spectrophotometry, agarose gel electrophoresis, restriction endonuclease analysis and PCR. The combined bead beating and enzymatic extraction method yielded more DNA. However, that method produced some sheared DNA, visible either by agarose gel electrophoresis or by restriction endonuclease analysis. All methods were appropriate for PCR amplification of a 123 bp fragment of IS6110 in M. bovis and M. tuberculosis, and of a 1700 bp fragment of FR300 region in M. avium avium. CONCLUSIONS:Combined bead beating and enzymatic extraction method was the most efficient and easy method for extracting DNA from bacteria of the M. tuberculosis complex. SIGNIFICANCE AND IMPACT OF THE STUDY:The results reveal important differences among the DNA extraction methods for mycobacteria, which are relevant for the success of further downstream molecular analysis.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Amaro A,Duarte E,Amado A,Ferronha H,Botelho Adoi
10.1111/j.1472-765X.2008.02372.xsubject
Has Abstractpub_date
2008-07-01 00:00:00pages
8-11issue
1eissn
0266-8254issn
1472-765Xpii
LAM2372journal_volume
47pub_type
杂志文章abstract::This study examines the diversity of streptomycetes causing potato scab using phenotypic identification, 16S rRNA sequence, and pathogenicity measurements. Pathogenicity did not correlate well with taxonomic identification, nor with phylogenetic position. This result suggests that neither phenotypic nor 16S characteri...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1998.t01-11-00449.x
更新日期:1998-11-01 00:00:00
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journal_title:Letters in applied microbiology
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doi:10.1111/j.1472-765X.2012.03230.x
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abstract:AIMS:The present study describes the implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry. METHODS AND RESULTS:Real-time PCR with Salmonella invA-specific primers and a standard bacteriological method was applied to detect Salmonella in tetrathionate enrichment cult...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01036.x
更新日期:2002-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/lam.12010
更新日期:2013-02-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01310.x
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abstract::Limited data are available on the prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in the UK. We tested 124 raw meat samples for MRSA including pork (n = 63), chicken (n = 50) and turkey (n = 11) collected from retail outlets in North West England between March and July 2015. MR...
journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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更新日期:2010-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1472-765x.2000.00714.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.02086.x
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pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.02060.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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更新日期:2007-10-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03123.x
更新日期:2011-10-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1994.tb00475.x
更新日期:1994-11-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13332
更新日期:2020-09-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1994.tb00458.x
更新日期:1994-11-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01977.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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更新日期:1998-08-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1993.tb01401.x
更新日期:1993-04-01 00:00:00
abstract::Five phage types have been identified in 38 strains of Salmonella enteritidis isolated in Turkey in the 20-month period June 1992-January 1994. Strains belonging to phage type 4 predominated. Within phage type 4, plasmid profile typing has proved a useful method of strain discrimination and has confirmed the identity ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1994.tb00960.x
更新日期:1994-10-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1995.tb00407.x
更新日期:1995-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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更新日期:2011-11-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1997.00225.x
更新日期:1997-10-01 00:00:00
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journal_title:Letters in applied microbiology
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更新日期:2015-10-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2008.02338.x
更新日期:2008-04-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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更新日期:2013-08-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1997.00178.x
更新日期:1997-08-01 00:00:00
abstract::The marine antimicrobial peptide NZ17074, a variant of arenicin-3 from Arenicola marina that has broad antimicrobial activity and high bioavailability, can be designed to treat bacterial and fungal diseases. To reduce the toxicity of NZ17074, N6 was designed by replacing a cysteine in positions 3 and 20 with alanine, ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12770
更新日期:2017-09-01 00:00:00