Protoplast formation and regeneration in Streptomyces flavopersicus.

abstract：AIM:To investigate the viability, surface characteristics and ability of spores of a Geobacillus sp. isolated from a milk powder production line to adhere to stainless steel surfaces before and after a caustic (NaOH) wash used in clean-in-place regimes. METHODS AND RESULTS:Exposing sessile spores to 1% NaOH at 65°C fo...

journal_title：Letters in applied microbiology

authors： Brent Seale R,Flint SH,James McQuillan A,Bremer PJ

更新日期：2011-02-01 00:00:00

abstract：:Flow cytometry in combination with fluorescent molecular markers 5- (and 6-) carboxyfluorescein succinimidylester (CFSE) and propidium iodide (PI) have been applied to determine lag times, numbers of cell divisions and injury after mild heat (50 degrees C, 5 min) and nisin treatments (0.1 and 1.0 microgram ml-1) of La...

journal_title：Letters in applied microbiology

authors： Ueckert JE,Nebe von-Caron G,Bos AP,ter Steeg PF

更新日期：1997-10-01 00:00:00

abstract：AIMS:To investigate the spatial organization of endogenous and exogenously applied Lactobacillus communities at specific locations in the adult gastrointestinal tract of different hosts. METHODS AND RESULTS:Samples of the human, murine and avian gastrointestinal tract of subjects that received or not received a Lactob...

journal_title：Letters in applied microbiology

authors： Lebeer S,Verhoeven TL,Claes IJ,De Hertogh G,Vermeire S,Buyse J,Van Immerseel F,Vanderleyden J,De Keersmaecker SC

更新日期：2011-03-01 00:00:00

abstract：AIMS:To compare a range of enrichment broths and enrichment temperatures for the isolation of Escherichia coli O157 by immunomagnetic separation (IMS) from sandy, loam and clay soils. METHODS AND RESULTS:Soils were spiked with cocktails of four atoxigenic strains of E. coli O157 and four strains of commensal E. coli. ...

journal_title：Letters in applied microbiology

authors： Hepburn NF,MacRae M,Johnston M,Mooney J,Ogden ID

更新日期：2002-01-01 00:00:00

abstract：AIMS:To assess the ability of staphylococcal bacteriophage K to inhibit Staphylococcus aureus in raw milk. METHODS AND RESULTS:The ability of bacteriophage (phage) to replicate in milk is important in situations where phage might be used as a therapeutic for bovine mastitis. Phage K was able to replicate normally, lea...

journal_title：Letters in applied microbiology

authors： O'Flaherty S,Coffey A,Meaney WJ,Fitzgerald GF,Ross RP

更新日期：2005-01-01 00:00:00

abstract：AIMS: To develop a specific and highly sensitive loop-mediated isothermal amplification (LAMP) technique for the rapid detection of canine parvovirus (CPV) DNA directly in suspected faecal samples of dogs by employing a simple method of template preparation. METHODS AND RESULTS: LAMP reaction was developed by designin...

journal_title：Letters in applied microbiology

authors： Mukhopadhyay HK,Amsaveni S,Matta SL,Antony PX,Thanislass J,Pillai RM

更新日期：2012-09-01 00:00:00

abstract：:Candida-associated denture stomatitis presents as erythema of the palatal mucosa and is caused by biofilms containing the fungus Candida albicans that co-reside with oral bacteria on the denture-fitting surface. This study aimed to assess the effect of several frequently encountered oral bacteria on the expression of ...

journal_title：Letters in applied microbiology

authors： Morse DJ,Wilson MJ,Wei X,Bradshaw DJ,Lewis MAO,Williams DW

更新日期：2019-04-01 00:00:00

abstract：:Quantitative and qualitative changes in organic matter content of wastewater effluents attributable to chlorination and ozonation have been analysed using bioassays as well as organic carbon direct measures. Bioassays were carried out using the bacterial populations of wastewater and two Escherichia coli strains as te...

journal_title：Letters in applied microbiology

authors： Arana I,Santorum P,Muela A,Barcina I

更新日期：2000-08-01 00:00:00

abstract：:A 21-mer DNA oligonucleotide probe targeting the 23S rRNA of Mycobacterium leprae was developed and its high specificity demonstrated by dot-blot hybridization. Even under relaxed hybridization and washing conditions (20 degrees C below Tm) the probe was highly selective in that positive signals were only detected wit...

journal_title：Letters in applied microbiology

authors： Liesack W,Pitulle C,Stackebrandt E

更新日期：1990-08-01 00:00:00

abstract：:Plant proteases play a fundamental role in several processes like growth, development and in response to biotic and abiotic stress. In particular, aspartic proteases (AP) are expressed in different plant organs and have antimicrobial activity. Previously, we purified an AP from Salpichroa origanifolia fruits called sa...

journal_title：Letters in applied microbiology

authors： Díaz ME,Rocha GF,Kise F,Rosso AM,Guevara MG,Parisi MG

更新日期：2018-08-01 00:00:00

abstract：AIMS:To propose a universal workflow of sample preparation method for the identification of highly pathogenic bacteria by MALDI-TOF MS. METHODS AND RESULTS:Fifteen bacterial species, including highly virulent Gram-positive (Bacillus anthracis and Clostridium botulinum) and Gram-negative bacteria (Brucella melitensis, ...

journal_title：Letters in applied microbiology

authors： Drevinek M,Dresler J,Klimentova J,Pisa L,Hubalek M

更新日期：2012-07-01 00:00:00

abstract：UNLABELLED:Newly synthesized erythromycin derivatives were screened for synergy with oxacillin and other β-lactam antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). MRSA ATCC43300 and some clinically isolated MRSA were used. Several erythromycin derivatives were found to possess high synergism with...

journal_title：Letters in applied microbiology

authors： Li Z,He M,Dong X,Lin H,Ge H,Shen S,Li J,Ye RD,Chen D

更新日期：2015-04-01 00:00:00

abstract：AIM:To evaluate the effect of starvation, heat, cold, acid, alkaline, chlorine and ethanol stresses on the resistance of Enterobacter sakazakii in powdered infant milk formula (PIMF) towards gamma radiation. METHODS AND RESULTS:Stressed cells of E. sakazakii ATCC 51329 and four other food isolate strains were mixed in...

journal_title：Letters in applied microbiology

authors： Osaili T,Al-Nabulsi A,Shaker R,Ayyash M,Olaimat A,Abu Al-Hasan A,Kadora K,Holley R

更新日期：2008-08-01 00:00:00

abstract：AIMS:The objective of this study was to identify compounds responsible for medicinal off-flavours produced by different species and strains of Alicyclobacillus in orange juice using a combination of chromatographic-coupled olfactometric techniques and gas chromatography-mass spectrometry (GC-MS). METHODS AND RESULTS:E...

journal_title：Letters in applied microbiology

authors： Gocmen D,Elston A,Williams T,Parish M,Rouseff RL

更新日期：2005-01-01 00:00:00

abstract：:Parabens have been widely used as antimicrobial preservatives in cosmetics, pharmaceuticals, foods and beverages. Commonly, methyl-, ethyl-, propyl- and butylparaben are used independently or in combination to maintain the quality of industrial products, and they are considered to have low toxicity. However, recent ev...

journal_title：Letters in applied microbiology

authors： Murata W,Yamaguchi Y,Fujita KI,Yamauchi K,Tanaka T,Ogita A

更新日期：2019-12-01 00:00:00

abstract：:A Coxiella sp. closely related to the agent of Q-fever, Coxiella burnetii, has been associated with mortalities in redclaw crayfish, (Cherax quadricarinatus), in farms and experimental facilities for three decades. Limited sequence data including 16S rRNA have placed the rickettsial species as a new species, candidatu...

journal_title：Letters in applied microbiology

authors： Ruth Elliman J,Owens L

更新日期：2020-09-01 00:00:00

abstract：:Currently used alum precipitated and oil adjuvant vaccines against HS caused by Pasteurella multocida B:2, have side effects and short-lived immunity, leading to regular catastrophic outbreaks in bovines in Asian subcontinent. The need for the development of an improved vaccine with longer immunity and the ability to ...

journal_title：Letters in applied microbiology

authors： Qureshi S,Saxena HM,Imam N,Kashoo Z,Sharief Banday M,Alam A,Malik MZ,Ishrat R,Bhat B

更新日期：2018-09-01 00:00:00

abstract：AIMS:To measure rates of release of small molecules during pressure germination of Bacillus subtilis spores, and the role of SpoVA proteins in dipicolinic acid (DPA) release. METHODS AND RESULTS:Rates of DPA release during B. subtilis spore germination with pressures of 150 or 500 megaPascals were much higher in spore...

journal_title：Letters in applied microbiology

authors： Vepachedu VR,Hirneisen K,Hoover DG,Setlow P

更新日期：2007-09-01 00:00:00

abstract：AIMS:This study aimed to identify a simple, inexpensive preservation technique that will allow a quick and reliable recovery of Campylobacter jejuni following long-term periods of preservation. METHODS AND RESULTS:Preservation techniques include (i) Cryobank microbial preservation system using hypertonic 'cryopreserva...

journal_title：Letters in applied microbiology

authors： Gorman R,Adley CC

更新日期：2004-01-01 00:00:00

abstract：AIMS:In traditional Thai medicine, nutgall of Quercus infectoria G. Olivier is well-documented as an effective agent for wound and skin infections. The present study was aimed to establish modes of action of the ethanol extract of the plant as well as its main constituents to induce anti-methicillin-resistant Staphyloc...

journal_title：Letters in applied microbiology

authors： Chusri S,Voravuthikunchai SP

更新日期：2011-06-01 00:00:00

abstract：AIMS:The aim of this study is to develop an RT-PCR assay combined with immunomagnetic beads (IMS/RT-PCR) coating monoclonal antibody (Mab) for separation and detection of norovirus (genogroup II) in faecal samples. We furthermore compare its detection limits with IMS/RT-PCR using polyclonal antibody (Pab) and the TRIzo...

journal_title：Letters in applied microbiology

authors： Yao L,Wu Q,Wang D,Kou X,Zhang J

更新日期：2009-08-01 00:00:00

abstract：:This study evaluated the performance of CPS ID2 (bioMérieux) compared to that of conventionally used selective agar for the identification of bacteria responsible for urinary tract infections. This medium detects bacterial enzymes using chromogenic substrates. Two hundred and nineteen samples of urine were tested in o...

journal_title：Letters in applied microbiology

authors： Willinger B,Manafi M

更新日期：1995-05-01 00:00:00

abstract：:The growth of pure cultures of Clostridium perfringens (ATCC 12922) and Cl. sporogenes (PA 3679) in five non-selective media, fluid thioglycollate medium (FTM), rapid perfringens medium (RPM), Columbia broth Malthus (CBM), reinforced clostridial medium (RCM) and lactose sulphite (LS), was monitored using conductance m...

journal_title：Letters in applied microbiology

authors： Dromigny E,Bourrion F,Rugraf Y,Bolton FJ,Leden N

更新日期：1997-01-01 00:00:00

abstract：:To investigate the potential activation of hydrogen peroxide by a novel catalyst, reducing the concentration of hydrogen peroxide required and the time taken for mycobactericidal activity. The mycobactericidal properties of an iron-based novel heterogeneous-modified polyacrylonitrile (PAN) catalyst in combination with...

journal_title：Letters in applied microbiology

authors： Price SL,Huddersman KD,Shen J,Walsh SE

更新日期：2013-02-01 00:00:00

abstract：AIMS:The objective of this work was to purify the tyrosinase from Bacillusthuringiensis subsp. kurstaki (Bt) (CCTCC AB 90010) and study its enzymatic properties. METHODS AND RESULTS:A 'one-step' purification method was used in this work, which was an easy, high-yield purification method. Tyrosinase activity of this pu...

journal_title：Letters in applied microbiology

authors： Liu N,Zhang T,Wang YJ,Huang YP,Ou JH,Shen P

更新日期：2004-01-01 00:00:00

abstract：AIM:To quantify the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 MG1655 cells at static temperatures. METHODS AND RESULTS:Stationary-phase E. coli cells were inactivated at 52, 54 and 58°C. The heat resistance is described as reduction in the inactivation rate, k(max) , and...

journal_title：Letters in applied microbiology

authors： Velliou EG,Van Derlinden E,Cappuyns AM,Aerts D,Nikolaidou E,Geeraerd AH,Devlieghere F,Van Impe JF

更新日期：2011-02-01 00:00:00

abstract：AIMS: The aim of this paper is to check the effect of salinity on the bioremediation process of petroleum hydrocarbons in the saline-alkaline soil. METHODS AND RESULTS: In this study, soil salinity was adjusted to different levels by water leaching method and the bioremediation process was conducted for 28 days. Soil ...

journal_title：Letters in applied microbiology

authors： Qin X,Tang JC,Li DS,Zhang QM

更新日期：2012-09-01 00:00:00

abstract：:From a genomic library of Mycoplasma hyopneumoniae a 1.3 kb DNA fragment was cloned which showed specific Southern hybridization and dot hybridization with the type strain of several porcine and bovine Mycoplasma species. This probe selectively recognized M. hyopneumoniae sequences in purified DNA or in broth-grown or...

journal_title：Letters in applied microbiology

authors： Ahrens P,Friis NF

更新日期：1991-06-01 00:00:00

abstract：UNLABELLED:Bioconversion of biomass, particularly crop wastes, into biofuels is being developed as an alternative approach in meeting the high energy demand. In this study, a thermophilic bacterial strain BY-3 that exhibits cellulolytic potential was isolated from faecal samples of Tibetan pigs; this strain was identif...

journal_title：Letters in applied microbiology

authors： Meng F,Ma L,Ji S,Yang W,Cao B

更新日期：2014-09-01 00:00:00

abstract：AIMS:The study evaluated the efficacy of four Mycobacterium avium subsp. paratuberculosis (MAP) culture media in suppressing commonly used starter cultures and typical nonstarter microflora present during the manufacture and ripening of Cheddar cheese, with a view to identify a suitable medium for the enumeration of MA...

journal_title：Letters in applied microbiology

authors： Donaghy JA,Totton NL,Rowe MT

更新日期：2003-01-01 00:00:00