Abstract:
AIMS:To measure rates of release of small molecules during pressure germination of Bacillus subtilis spores, and the role of SpoVA proteins in dipicolinic acid (DPA) release. METHODS AND RESULTS:Rates of DPA release during B. subtilis spore germination with pressures of 150 or 500 megaPascals were much higher in spores with elevated levels of SpoVA proteins, and spores with a temperature-sensitive mutation in the spoVA operon were temperature-sensitive in DPA release during pressure germination. Spores also released arginine and glutamic acid, but not AMP, during pressure germination. CONCLUSIONS:Pressure germination of B. subtilis spores causes release of many small molecules including DPA. SpoVA proteins are involved in the release of DPA, perhaps because SpoVA proteins are a component of a DPA channel in the spore's inner membrane. SIGNIFICANCE AND IMPACT OF THE STUDY:This work provides new insight into the mechanism of pressure germination of spores of Bacillus species, a process that has significant potential for usage in the food industry.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Vepachedu VR,Hirneisen K,Hoover DG,Setlow Pdoi
10.1111/j.1472-765X.2007.02204.xsubject
Has Abstractpub_date
2007-09-01 00:00:00pages
342-8issue
3eissn
0266-8254issn
1472-765Xpii
LAM2204journal_volume
45pub_type
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