Detection and characterization of Clostridium difficile in retail chicken.

abstract：AIMS:To examine factors affecting faecal shedding of the foodborne pathogens Escherichia coli O157:H7 and Salmonella in dairy cattle and evaluate antimicrobial susceptibility of these isolates. METHODS:Faecal samples were obtained in replicate from lactating (LAC; n = 60) and non-lactating (NLAC; n = 60) Holstein catt...

journal_title：Letters in applied microbiology

authors： Fitzgerald AC,Edrington TS,Looper ML,Callaway TR,Genovese KJ,Bischoff KM,McReynolds JL,Thomas JD,Anderson RC,Nisbet DJ

更新日期：2003-01-01 00:00:00

abstract：:The development of various types of virus-like particles (VLPs) has accelerated over the past two decades as the importance of VLPs for generating next-generation vaccines has been appreciated. Yeast has advantages such as scalable fermentation, low risk of contamination by adventitious agents, low production costs an...

journal_title：Letters in applied microbiology

authors： Kim HJ,Kim HJ

更新日期：2017-02-01 00:00:00

abstract：:Five phage types have been identified in 38 strains of Salmonella enteritidis isolated in Turkey in the 20-month period June 1992-January 1994. Strains belonging to phage type 4 predominated. Within phage type 4, plasmid profile typing has proved a useful method of strain discrimination and has confirmed the identity ...

journal_title：Letters in applied microbiology

authors： Erdem B,Threlfall EJ,Schofield SL,Ward LR,Rowe B

更新日期：1994-10-01 00:00:00

abstract：:Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a worldwide concern. Cattle are their main reservoir and may contaminate watercourses through manure. We characterized a collection of 38 STEC O157:H7 strains isolated from surface water in feedlots areas (puddles inside pens formed after the rainfall or by spil...

journal_title：Letters in applied microbiology

authors： Tanaro JD,Pianciola LA,D'Astek BA,Piaggio MC,Mazzeo ML,Zolezzi G,Rivas M

更新日期：2018-06-01 00:00:00

abstract：:An investigation of the biochemical, nutritional and pathogenic reactions of strains of Pseudomonas syringae pv. japonica and Ps. syringae pv. syringae showed them to be indistinguishable. Pseudomonas syringae pv. japonica is a junior synonym of Ps. syringae pv. syringae. ...

journal_title：Letters in applied microbiology

authors： Young JM

更新日期：1992-10-01 00:00:00

abstract：:Bulk tank milk from 160 producers was tested for Listeria monocytogenes at three monthly intervals over 1 year. Twenty-five producers were positive, most on a single occasion, only seven were positive on three or more of the four samplings. Listeria monocytogenes numbers were low, usually < 1 ml-1, the highest was 35 ...

journal_title：Letters in applied microbiology

authors： Fenlon DR,Stewart T,Donachie W

更新日期：1995-01-01 00:00:00

abstract：AIMS:The aim of this study is to develop an RT-PCR assay combined with immunomagnetic beads (IMS/RT-PCR) coating monoclonal antibody (Mab) for separation and detection of norovirus (genogroup II) in faecal samples. We furthermore compare its detection limits with IMS/RT-PCR using polyclonal antibody (Pab) and the TRIzo...

journal_title：Letters in applied microbiology

authors： Yao L,Wu Q,Wang D,Kou X,Zhang J

更新日期：2009-08-01 00:00:00

abstract：:Nanobacteria, also known as calcifying nanoparticles (CNP), are controversial infectious agents not matching the current criteria for 'living organism'. Despite the controversy of their classification, they propagate and cause cell death in vitro and are associated or found in many human diseases. Thus, more efforts s...

journal_title：Letters in applied microbiology

authors： Kajander EO

更新日期：2006-06-01 00:00:00

abstract：:A native isolate Lactobacillus farciminis MD isolated from fermenting mushroom exhibited a high degree of sensitivity to the majority of the bacteriocins produced by strains of lactobacilli, leuconostoc and pediococci. Also, the efficacy of Lact. farciminis MD as a sensitive strain for antibiotic assay was established...

journal_title：Letters in applied microbiology

authors： Halami PM,Chandrashekar A,Nand K

更新日期：2000-03-01 00:00:00

abstract：AIMS:The aim of this study was to determine the antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20 against several micro-organisms, including Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi. METHODS AND RESULTS:Antimicrobial and ...

journal_title：Letters in applied microbiology

authors： Gudiña EJ,Rocha V,Teixeira JA,Rodrigues LR

更新日期：2010-04-01 00:00:00

abstract：AIMS:To improve the production of sweet-tasting protein brazzein in Lactococcus lactis using controlled fermentation conditions. METHODS AND RESULTS:The nisin-controlled expression system was used for brazzein expression. The concentration of nisin for induction and the optical density (OD) at induction were therefore...

journal_title：Letters in applied microbiology

authors： Berlec A,Tompa G,Slapar N,Fonović UP,Rogelj I,Strukelj B

更新日期：2008-02-01 00:00:00

abstract：:Samples were collected from 148 adult cats, processed for isolation of Staphylococcus species and tested for susceptibility to penicillin G, gentamicin, oxacillin, amoxycillin, ampicillin, cephalexin and rifampin. Methicillin resistance was also determined. Ninety-eight isolates were obtained (66% of samples). Coagula...

journal_title：Letters in applied microbiology

authors： Lilenbaum W,Nunes EL,Azeredo MA

更新日期：1998-10-01 00:00:00

abstract：:This study examines the diversity of streptomycetes causing potato scab using phenotypic identification, 16S rRNA sequence, and pathogenicity measurements. Pathogenicity did not correlate well with taxonomic identification, nor with phylogenetic position. This result suggests that neither phenotypic nor 16S characteri...

journal_title：Letters in applied microbiology

authors： Bramwell PA,Wiener P,Akkermans AD,Wellington EM

更新日期：1998-11-01 00:00:00

abstract：UNLABELLED:High-pressure homogenization (HPH) has been proposed to be applied directly to lactic acid bacterial cells at sublethal levels to enhance some functional properties. As the principal target of HPH are the cell surface envelope structures, the aim of this work was to study the effect of a HPH treatment, appli...

journal_title：Letters in applied microbiology

authors： Tabanelli G,Patrignani F,Gardini F,Vinderola G,Reinheimer J,Grazia L,Lanciotti R

更新日期：2014-02-01 00:00:00

abstract：AIMS:This study aimed to identify a simple, inexpensive preservation technique that will allow a quick and reliable recovery of Campylobacter jejuni following long-term periods of preservation. METHODS AND RESULTS:Preservation techniques include (i) Cryobank microbial preservation system using hypertonic 'cryopreserva...

journal_title：Letters in applied microbiology

authors： Gorman R,Adley CC

更新日期：2004-01-01 00:00:00

abstract：:Heat-resistant fungi occur sporadically and are a continuing problem for the food and beverage industry. The genus Talaromyces, as a typical fungus, is capable of producing the heat-resistant ascospores responsible for the spoilage of processed food products. Isocitrate lyase, a signature enzyme of the glyoxylate cycl...

journal_title：Letters in applied microbiology

authors： Yamashita S,Nakagawa H,Sakaguchi T,Arima TH,Kikoku Y

更新日期：2018-01-01 00:00:00

abstract：:The specific detection and enumeration of Lactobacillus brevis LB62, Carnobacterium divergens V14 and Carnobacterium piscicola VI were studied by in situ hybridization-flow cytometry. The method was performed on the exponential growth phase with three probes targeting 16S rRNA labelled with fluorescein isothicyanate (...

journal_title：Letters in applied microbiology

authors： Connil N,Dousset X,Onno B,Pilet MF,Breuil MF,Montel MC

更新日期：1998-11-01 00:00:00

abstract：:Sixteen Bifidobacterium isolates from the human gastrointestinal tract were assayed for susceptibility to 44 antibiotics by soft agar overlay disc diffusion on TPY agar. Five isolates (3/7 B. bifidum and 2/3 B. breve) exhibited atypical antibiotic susceptibility profiles. Poor growth in the agar overlay accounted for ...

journal_title：Letters in applied microbiology

authors： Charteris WP,Kelly PM,Morelli L,Collins JK

更新日期：1998-05-01 00:00:00

abstract：:The aim of this study was to determine the epidemiology (location, microbial load, microbiome, presence/absence of biofilm and pathogens, including ESKAPE-Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter species, and antimicrobial susc...

journal_title：Letters in applied microbiology

authors： Costa DM,Johani K,Melo DS,Lopes LKO,Lopes Lima LKO,Tipple AFV,Hu H,Vickery K

更新日期：2019-04-01 00:00:00

abstract：AIM:To identify the gene that encodes nigrescin, a bacteriocin produced by Prevotella nigrescens ATCC 25261. METHODS AND RESULTS:Each open reading frame (ORF) of the nig gene cluster (nigA, nigB, nigC and nigD) was transferred into an expression vector. The recombinant proteins encoded by nigA, nigB, nigC and nigD wer...

journal_title：Letters in applied microbiology

authors： Teanpaisan R,Narawatthana S,Utarabhand P

更新日期：2009-09-01 00:00:00

abstract：:Quillaja saponin (QS) was incubated at 39 degrees C in an in vitro medium containing rumen liquor from a cow fed a roughage diet. No degradation of QS was observed up to 6 h of fermentation. Incubation for 9, 12 and 24 h decreased the content of QS by 16%, 45% and 100%. The content of QS did not decrease when incubate...

journal_title：Letters in applied microbiology

authors： Makkar HP,Becker K

更新日期：1997-10-01 00:00:00

abstract：AIMS:Strains of Clostridium butyricum have been increasingly used as probiotics for both animals and humans. The aim of this study was to develop a growth medium for cultivating C. butyricum ZJUCB using a statistical methodology. METHODS AND RESULTS:Response surface methodology (RSM) was used to evaluate the effects o...

journal_title：Letters in applied microbiology

authors： He GQ,Kong Q,Ding LX

更新日期：2004-01-01 00:00:00

abstract：:Candida albicans is an opportunistic fungus, which causes vulvovaginal candidiasis (VVC). The aim of this study was to evaluate Mrr2 mutation and its expression levels and Candida drug resistance 1 (Cdr1) in C. albicans associated with fluconazole (FCA) resistance. We identified 80 isolates of C. albicans from 155 vag...

journal_title：Letters in applied microbiology

authors： Feng W,Yang J,Ji Y,Xi Z,Yang L,Zhu X,Ma Y

更新日期：2020-02-01 00:00:00

abstract：:The L-2,3-butanediol dehydrogenase (L-BDH) gene of Brevibacterium saccharolyticum was strongly expressed in Escherichia coli using the tac promoter. However, the stereospecificity of the resulting L-BDH was reduced. The region upstream of the meso-BDH gene of Klebsiella pneumoniae was also involved in the expression o...

journal_title：Letters in applied microbiology

authors： Ui S,Takusagawa Y,Ohtsuki T,Mimura A,Ohkuma M,Kudo T

更新日期：2001-02-01 00:00:00

abstract：:A series of experiments was conducted to identify the molecular species responsible for surface active emulsification (surfactant) bioactivity in Bacillus subtilis subsp. subtilis strain ATCC PTA-125135, and to describe culture conditions to support the enriched production of said bioactivity in cultured plaque of the...

journal_title：Letters in applied microbiology

authors： Krueger LA,Grzemski MA,Bilyeu MC,Horst JG,Ugrin SA,Hosette CA,Spangler DA,Ayangbile GA

更新日期：2020-11-01 00:00:00

abstract：:A Multiplex PCR-based assay (m-PCR) with three sets of primers was developed for the detection of all serotypes of Salmonella enterica and the identification of Salmonella Enteritidis and Salmonella Typhimurium. This method was evaluated against a bacteriological method for the analysis of environmental swabs of poult...

journal_title：Letters in applied microbiology

authors： Soumet C,Ermel G,Rose N,Rose V,Drouin P,Salvat G,Colin P

更新日期：1999-02-01 00:00:00

abstract：:Heat stable antifungal factor (HSAF) is considered to be a potential biological pesticide due to its broad antifungal activity and novel mode of action. However, few studies have reported on HSAF production during fermentation. Thus, this work was executed to optimize the medium composition to maximize HSAF production...

journal_title：Letters in applied microbiology

authors： Tang B,Zhao YC,Shi XM,Xu HY,Zhao YY,Dai CC,Liu FQ

更新日期：2018-05-01 00:00:00

abstract：AIMS:To evaluate the accuracy of pyrosequencing for the description of Phytophthora communities in terms of taxa identification and risk of assignment for false Molecular Operational Taxonomic Units (MOTUs). METHODS AND RESULTS:Pyrosequencing of Internal Transcribed Spacer 1 (ITS1) amplicons was used to describe the s...

journal_title：Letters in applied microbiology

authors： Vettraino AM,Bonants P,Tomassini A,Bruni N,Vannini A

更新日期：2012-11-01 00:00:00

abstract：UNLABELLED:Assessment of antibacterial as well as antiherpes virus activity of sulfonoquinovosyldiacylglyceride (SQDG), a glycolipid, isolated from the leaves of Azadirachta indica has been described. Antimicrobial activity was evaluated against Gram-positive, Gram-negative bacteria and herpes simplex virus. SQDG showe...

journal_title：Letters in applied microbiology

authors： Bharitkar YP,Bathini S,Ojha D,Ghosh S,Mukherjee H,Kuotsu K,Chattopadhyay D,Mondal NB

更新日期：2014-02-01 00:00:00

abstract：:The small cryptic plasmid pMB1 (1.9 kb), previously isolated from Bifidobacterium longum, has been characterized by physical mapping. Two cloning vectors, pMR3 and pDG7, carrying chloramphenicol and ampicillin resistances derived from pJH101, have been electroporated in Escherichia coli. ...

journal_title：Letters in applied microbiology

authors： Matteuzzi D,Brigidi P,Rossi M,Di D

更新日期：1990-10-01 00:00:00