Abstract:
:The L-2,3-butanediol dehydrogenase (L-BDH) gene of Brevibacterium saccharolyticum was strongly expressed in Escherichia coli using the tac promoter. However, the stereospecificity of the resulting L-BDH was reduced. The region upstream of the meso-BDH gene of Klebsiella pneumoniae was also involved in the expression of the B. saccharolyticum gene. However, in this case, the resulting L-BDH exhibited more stable stereospecificity. A stereospecificity recognition region was located within the rear sequence (Hpa I site, carboxy terminal) of the BDH open reading frame. Using a transformed strain of E. coli, the conversion of L-acetoin (L-AC), in the commercially available racemic mixture of AC, to L-2,3-butanediol (L-BD) was attempted. As a result, 0.37% L-BD was formed from 1% AC added to the culture.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Ui S,Takusagawa Y,Ohtsuki T,Mimura A,Ohkuma M,Kudo Tdoi
10.1046/j.1472-765x.2001.00869.xsubject
Has Abstractpub_date
2001-02-01 00:00:00pages
93-8issue
2eissn
0266-8254issn
1472-765Xpii
lam869journal_volume
32pub_type
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更新日期:1995-01-01 00:00:00
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journal_title:Letters in applied microbiology
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doi:10.1046/j.1472-765x.2003.01291.x
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更新日期:2015-05-01 00:00:00