Abstract:
:This study examines the diversity of streptomycetes causing potato scab using phenotypic identification, 16S rRNA sequence, and pathogenicity measurements. Pathogenicity did not correlate well with taxonomic identification, nor with phylogenetic position. This result suggests that neither phenotypic nor 16S characteristics are diagnostic for scab-causing strains. Additionally, phenotypic characterizations were not fully consistent with phylogenetic relationships. This incongruency highlights the difficulties of taxonomy within the streptomycetes and affirms the need for better integration of genetic and phenotypic characters in the systematics of this group.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Bramwell PA,Wiener P,Akkermans AD,Wellington EMdoi
10.1046/j.1472-765x.1998.t01-11-00449.xsubject
Has Abstractpub_date
1998-11-01 00:00:00pages
255-60issue
5eissn
0266-8254issn
1472-765Xjournal_volume
27pub_type
杂志文章abstract::The detection of foodborne viruses in bivalve molluscs is a challenging procedure in relation to low virus concentration and to the presence of significant RT-PCR inhibitors. The aim of this study was the development of an efficient direct extraction method for foodborne viral RNA from bivalve molluscs. Using Mengovir...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13065
更新日期:2018-11-01 00:00:00
abstract::The recent rise in the awareness of the occurrence of toxic cyanobacterial blooms in aquatic environments, with associated human health problems and animal deaths, has increased the need for rapid, reliable and sensitive methods of determining cyanobacterial toxicity. A luminescent bacterial toxicity test was assessed...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1990.tb00161.x
更新日期:1990-10-01 00:00:00
abstract:AIMS:The study evaluated the efficacy of four Mycobacterium avium subsp. paratuberculosis (MAP) culture media in suppressing commonly used starter cultures and typical nonstarter microflora present during the manufacture and ripening of Cheddar cheese, with a view to identify a suitable medium for the enumeration of MA...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01392.x
更新日期:2003-01-01 00:00:00
abstract::Using a novel capillary thermal cycler, randomly amplified polymorphic DNA (RAPD) generated DNA fingerprints were obtained in 3 h. The RAPD profiles were produced using a random 10-mer primer (5'-ACCGCCTGCT-3') which discriminated between different Listeria spp. Unique fragment profiles of Listeria monocytogenes serot...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1995.tb00424.x
更新日期:1995-03-01 00:00:00
abstract::Fresh-cut produce is at greater risk of Salmonella contamination. Detection and early warning systems play an important role in reducing the dissemination of contaminated products. One-step Reverse Transcription Polymerase Chain Reaction (RT-qPCR) targeting Salmonella tmRNA with or without a 6-h enrichment was evaluat...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12871
更新日期:2018-05-01 00:00:00
abstract:AIMS:The risk of hepatitis C virus infection in hospital environments can be assessed not only by studying epidemiological data and work practices, but also by the detection of these viruses (or indicators thereof) in health-care settings, on instruments etc. METHODS:Since standardized techniques specific to this end ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01066.x
更新日期:2002-01-01 00:00:00
abstract::Actinomyces viscosus strain GA produces an exocellular biological retardant(s) that prevents certain vegetable plants from becoming overgrown. The biological retardant(s) was assayed using the etiolated wheat coleoptile assay, and fractionation of culture supernatant fluid resulted in a partial purification of the ret...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1997.00178.x
更新日期:1997-08-01 00:00:00
abstract:AIM:To study the effect of glucose concentrations on the growth by Brettanomyces bruxellensis yeast strain in batch experiments and develop a mathematical model for kinetic behaviour analysis of yeast growing in batch culture. METHODS AND RESULTS:A Matlab algorithm was developed for the estimation of model parameters....
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03081.x
更新日期:2011-08-01 00:00:00
abstract:AIMS:To evaluate the in vitro bactericidal activity of the novel antimicrobial peptide (AP) CECT7121 against Gram-positive bacteria from mastitic dairy cattle. METHODS AND RESULTS:A total of 15 Staphylococcus aureus, 10 Streptococcus dysgalactiae, 7 Strep. uberis, 1 Strep. agalactiae strains were isolated from 33 diff...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2008.02507.x
更新日期:2009-02-01 00:00:00
abstract:AIM:To compare the incidence of antimicrobial resistance among Salmonella serotypes isolated in a pig slaughterhouse in Zaragoza (Spain) during 1993 and 2001. METHODS AND RESULTS:A total of 168 isolates representing 10 serotypes were examined by disc diffusion method using 17 antibiotics. Data showed that the majority...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2005.01710.x
更新日期:2005-01-01 00:00:00
abstract::A lead-resistant bacteria isolated from soil contaminated with car battery waste were identified as Providencia alcalifaciens based on biochemical characteristics, FAME profile and 16S rRNA sequencing and designated as strain 2EA. It resists lead nitrate up to 0·0014 mol l(-1) by precipitating soluble lead as insolubl...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12026
更新日期:2013-02-01 00:00:00
abstract:AIMS:To determine which citric acid-producing strain of Aspergillus niger utilized wet corn distillers grains most effectively to produce citric acid. METHODS AND RESULTS:Citric acid and biomass production by the fungal strains were analysed on the untreated grains or autoclaved grains using an enzyme assay and a grav...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01958.x
更新日期:2006-09-01 00:00:00
abstract::Enterohaemorrhagic Escherichia coli (EHEC) agar was evaluated for its ability to recover one isolate of each of three serotypes (O157:H7, O26 and O113:H21) of shiga toxin-producing E. coli (STEC) from raw mince, pasteurized milk and salami after enrichment. The method detected around one colony-forming unit (cfu) in 2...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2000.00676.x
更新日期:2000-02-01 00:00:00
abstract::In a crossover study, volunteers consumed a diet supplemented with either wheat or rye bread. Levels of faecal mucus were determined and the adhesion of five selected probiotics to immobilized intestinal mucus, was assessed. No significant difference in the concentration of faecal mucus or adhesion of the probiotics t...
journal_title:Letters in applied microbiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1472-765x.2000.00758.x
更新日期:2000-07-01 00:00:00
abstract:AIMS:To screen a pair of primers suitable for denaturing gradient gel electrophoretic (DGGE) analysis of ruminal methanogenic Archaea and to detect the archaeal communities in the rumen of goat. METHODS AND RESULTS:Nine primer pairs for 16S rDNA of methanogenic Archaea, including six for directed polymerase chain reac...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02583.x
更新日期:2009-05-01 00:00:00
abstract:AIMS:To determine the underlying substrate utilization mechanism in the logistic equation for batch microbial growth by revealing the relationship between the logistic and Monod kinetics. Also, to determine the logistic rate constant in terms of Monod kinetic constants. METHODS AND RESULTS:The logistic equation used t...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2008.02537.x
更新日期:2009-04-01 00:00:00
abstract:AIM:The automated TEMPO system (bioMerieux) is based on the most probable number (MPN) method for the enumeration of micro-organisms in foods. In this study, we evaluated the performance of the TEMPO system as a diagnostic tool in comparison with the standard method in processed soy products. METHODS AND RESULTS:A ver...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03143.x
更新日期:2011-11-01 00:00:00
abstract::Staphylococcus aureus is a major agent of bovine mastitis in dairy herds, causing economic losses in dairy industry worldwide. In addition, milk and milk-products contaminated by Staph. aureus can cause harmful human diseases. The aim of this study was to characterize Staph. aureus strains isolated from dairy farms in...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12672
更新日期:2016-12-01 00:00:00
abstract::Nanobacteria, also known as calcifying nanoparticles (CNP), are controversial infectious agents not matching the current criteria for 'living organism'. Despite the controversy of their classification, they propagate and cause cell death in vitro and are associated or found in many human diseases. Thus, more efforts s...
journal_title:Letters in applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1472-765X.2006.01945.x
更新日期:2006-06-01 00:00:00
abstract:AIM: This report describes the use of a six-gene multi-locus sequence analysis (MLSA) to correctly identify Vibrio strains of the Harveyi clade. METHODS AND RESULTS: Vibrio isolates were characterized using a six housekeeping gene MLSA. The study provided evidence supporting: (i) a substantial number of reference st...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03183.x
更新日期:2012-02-01 00:00:00
abstract:UNLABELLED:Syphilis is a multistage sexually transmitted disease that remains a serious public health concern worldwide. The coexistence of Treponema pallidum with other closely related members of spirochaeta, such as Leptospira spp. and Borrelia burgdorferi, has complicated the serodiagnosis due to cross-reactive anti...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12554
更新日期:2016-04-01 00:00:00
abstract:AIMS:To examine the efficacy of liposome oral administration to induce systemic and mucosal immune responses against verotoxin-producing Escherichia coli (VTEC) and the effect of the induced antibodies on the binding of the bacteria to Caco-2 cells. METHODS AND RESULTS:Mice were immunized orally with VTEC antigen and ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01278.x
更新日期:2003-01-01 00:00:00
abstract::A monoclonal antibody (mAb) has been produced to aflatoxin B1 (AF B1) after successful immunization of mice and fusion of sensitized spleen cells with myeloma cancer cells. The mice were immunized with AF B1-oxime-protein conjugate. Positive mAbs were screened using an indirect ELISA specific for AF B1. The selected m...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1990.tb00106.x
更新日期:1990-04-01 00:00:00
abstract::Leclercia adecarboxylata, a Gram-negative bacillus of family Enterobacteriaceae, is an uncommonly identified pathogen isolated from environmental and clinical specimens. Most of the human infections are polymicrobial and commonly occur in immunocompromised hosts, although nosocomial infections in immunocompetent hosts...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12811
更新日期:2018-01-01 00:00:00
abstract:AIMS:Transmission of microbial pathogens to patients from water in dental units is a concern. To reduce this risk, the decontaminating efficiency of hydrogen peroxide was evaluated. METHODS AND RESULTS:Three percent hydrogen peroxide diluted 1 : 4 in distilled water (contact time 15 min) was used daily to disinfect th...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01378.x
更新日期:2003-01-01 00:00:00
abstract:AIM:To study genetic diversity of Chromobacterium haemolyticum isolates recovered from a natural tropical lake. METHODS AND RESULTS:A set of 31 isolates were recovered from a bacterial freshwater community by conventional plating methods and subjected to genetic and phenotypic characterization. The 16S ribosomal RNA (...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03052.x
更新日期:2011-06-01 00:00:00
abstract:AIMS:The aim of this study was to detect the main pathogenic human RNA enteric viruses able to persist in the environment such as astrovirus, enterovirus, norovirus and hepatitis A virus (HAV) in shellfish collected from two locations in northern Tunisia. METHODS AND RESULTS:Viruses were eluted from digestive tissues ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01978.x
更新日期:2006-10-01 00:00:00
abstract:AIMS:The following polymers were developed: polyethylene (PE), a PE and polyethylene oxide (70% PE and 30% PEO; PE + PEO) blend, PE and nisin (PE + nisin), PE, nisin, and EDTA (PE + nisin + EDTA), and PE + PEO with nisin (PE + PEO + nisin). METHODS AND RESULTS:Of the polymers tested, PE and PE + PEO did not exhibit an...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.01005.x
更新日期:2001-10-01 00:00:00
abstract:UNLABELLED:Chrysanthemum plants are subject to serious virus diseases, so detection and identification of virus pathogens is important to prevent the virus spread. A reliable one-step multiplex RT-PCR was developed to simultaneously detect two viruses and two viriods: chrysanthemum virus B, tomato Aspermy virus, chrysa...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12007
更新日期:2013-01-01 00:00:00
abstract:AIMS:The aim of this study was to elucidate if the need for iron for Escherichia coli to remain cultivable in a poorly nutritive medium such as the drinking water uses the iron transport system via the siderophores. METHODS AND RESULTS:Environmental strains of E. coli (isolated from a drinking water network), referenc...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01895.x
更新日期:2006-07-01 00:00:00