Analysis of pristinamycin-resistant Staphylococcus epidermidis isolates in the Tunisian Bone Marrow Transplant Center.


AIMS:We report the analysis of genetic determinants conferring resistance to pristinamycin in Staphylococcus epidermidis strains and epidemiology typing of these strains by pulsed-field gel electrophoresis. METHODS AND RESULTS:Staphylococcus epidermidis (346 isolates) were searched for strains with pristinamycin resistance. Pristinamycin-resistant strains (seven isolates) were isolated in five patients with haematological cancer in the Bone Marrow Transplant Centre of Tunisia in 2002. Resistance to pristinamycin was observed in 2% of isolates. The seven pristinamycin-resistant strains shared resistance to oxacillin (MIC = 8-512 microg ml(-1)), gentamicin (MIC = 16-512 microg ml(-1)), erythromycin (MIC > 1024 microg ml(-1)), lincomycin (MIC > 1024 microg ml(-1)), pristinamycin (MIC = 4-16 microg ml(-1)) and rifampin (MIC = 128-256 microg ml(-1)). erm genes were amplified: ermA from six strains and ermC from one. vga gene encoding streptogramins A resistance (pristinamycin résistance) was amplified from all strains and typed as vgaA by analysis after electrophoresis of restriction profiles of vga amplicons (two fragments with Sau3A of 164 and 378 bp; one fragment with EcoRI). Pulsed-field gel electrophoresis (PFGE) of SmaI chromosomal DNA digests of the seven S. epidermidis isolates divided them into two distinct pattern types: pulsed-field type A (classified from A1 to A6 subtypes) and type B. The six strains harbouring ermA genes belonged to the PFGE type A while the strain harbouring ermC genes belonged to the PFGE type B. We characterized an epidemic strain carrying the vgaA and ermA genes responsible for the outbreak. CONCLUSIONS:Two clones of pristinamycin-resistant S. epidermidis were isolated in our patients. One of them, isolated in all patients, had expanded over six months suggesting acquisition by cross-contamination. SIGNIFICANCE AND IMPACT OF THE STUDY:Increasing isolation of pristinamycin resistant S. epidermidis strains is an alarming indicator of nosocomial dissemination. The vector will be determined to establish a system of epidemiological surveillance.


Lett Appl Microbiol


Achour W,Bouchami O,Galopin S,Leclercq R,Ben Hassen A




Has Abstract


2008-03-01 00:00:00














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    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Katase M,Tsumura K

    更新日期:2011-11-01 00:00:00

  • Effect of Lactobacillus isolates on the adhesion of pathogens to chicken intestinal mucus in vitro.

    abstract:AIMS:The aims of this study were to investigate in vitro the effects of Lactobacillus isolates from a chicken on adhesion of pathogenic Salmonella and Escherichia coli to chicken intestinal mucus obtained from different intestinal regions. METHODS AND RESULTS:Bacteria were labelled by using methyl-1,2-[(3)H]-thymidine...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Ma YL,Guo T,Xu ZR,You P,Ma JF

    更新日期:2006-04-01 00:00:00

  • Genetic heterogeneity of non-O1 and non-O139 Vibrio cholerae isolates from shrimp aquaculture system: a comparison of RS-, REP- and ERIC-PCR fingerprinting approaches.

    abstract:AIMS:The genetic diversity of Vibrio cholerae isolated from black tiger shrimp (Penaeus monodon) aquaculture farms was determined using three PCR typing methods based on enterobacterial repetitive intergenic consensus (ERIC) sequences, ribosomal gene spacer (RS) sequence and repetitive extragenic palindromic (REP) sequ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Rao BM,Surendran PK

    更新日期:2010-07-01 00:00:00

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    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


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    更新日期:2015-08-01 00:00:00

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    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Rowan NJ,Espie S,Harrower J,Farrell H,Marsili L,Anderson JG,MacGregor SJ

    更新日期:2008-01-01 00:00:00

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    pub_type: 杂志文章


    authors: Li K,West TP

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  • Cloning, expression and characterization of attachment-invasion locus protein (Ail) of Yersinia enterocolitica and its utilization in rapid detection by immunoassays.

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    pub_type: 杂志文章


    authors: Balakrishna K,Murali HS,Batra HV

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Ben Said M,Abbassi MS,Bianchini V,Sghaier S,Cremonesi P,Romanò A,Gualdi V,Hassen A,Luini MV

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    pub_type: 杂志文章


    authors: Sakagami Y,Mimura M,Kajimura K,Yokoyama H,Linuma M,Tanaka T,Ohyama M

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    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Rogers JV,Richter WR,Shaw MQ,Choi YW

    更新日期:2008-10-01 00:00:00

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    pub_type: 杂志文章


    authors: Plutzer J,Törökné A,Karanis P

    更新日期:2010-01-01 00:00:00

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    pub_type: 杂志文章


    authors: Lyutzkanova D,Nikolova B,Stoilova-Discheva M,Todorov T

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    pub_type: 杂志文章


    authors: Xian M,Nie J,Meng Q,Liu J,Zhou C,Kang Y,Zhen K

    更新日期:2003-01-01 00:00:00

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    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Hope R,Magan N

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    pub_type: 杂志文章


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    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Brooks T,Keevil CW

    更新日期:1997-03-01 00:00:00

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    authors: Quang Le H,Suffredini E,Tien Pham D,Kim To A,De Medici D

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    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


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    更新日期:1999-05-01 00:00:00

  • Optimization of glycosidases production by Pseudoalteromonas issachenkonii KMM 3549(T).

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    pub_type: 杂志文章


    authors: Alexeeva YV,Ivanova EP,Bakunina IY,Zvaygintseva TN,Mikhailov VV

    更新日期:2002-01-01 00:00:00

  • Characterization of Bacillus thuringiensis serovar bolivia (serotype H63), a novel serovar isolated from the Bolivian high valleys.

    abstract::The type strain Bacillus thuringiensis var. bolivia (serotype H63), isolated from the Bolivian high valleys, has been characterized at different levels. Its parasporal crystal has an unusual shape and it is composed of a protein of 155 kDa which shows two bands of 75 and 80 kDa after activation. Analysis by PCR shows ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Ferrandis MD,Andrew R,Porcar M,Iriarte J,Cosmao-Dumanoir V,Lecadet MM,Caballero P,Ferré J

    更新日期:1999-06-01 00:00:00

  • A rapid staining technique for the detection of the initiation of germination of bacterial spores.

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    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Hamouda T,Shih AY,Baker JR Jr

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  • High organic loading influences the physical characteristics of aerobic sludge granules.

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    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Moy BY,Tay JH,Toh SK,Liu Y,Tay ST

    更新日期:2002-01-01 00:00:00

  • Rapid and sensitive detection of Vibrio cholerae by loop-mediated isothermal amplification targeted to the gene of outer membrane protein ompW.

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    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Srisuk C,Chaivisuthangkura P,Rukpratanporn S,Longyant S,Sridulyakul P,Sithigorngul P

    更新日期:2010-01-01 00:00:00

  • Pyruvate relieves the necessity of high induction levels of catalase and enables Campylobacter jejuni to grow under fully aerobic conditions.

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    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Verhoeff-Bakkenes L,Arends AP,Snoep JL,Zwietering MH,de Jonge R

    更新日期:2008-03-01 00:00:00

  • Prevalence of thermotolerant Campylobacter in partridges (Perdix perdix).

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    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Dipineto L,Gargiulo A,De Luca Bossa LM,Rinaldi L,Borrelli L,Santaniello A,Menna LF,Fioretti A

    更新日期:2009-09-01 00:00:00

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    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Lopes M,Gomes N,Gonçalves C,Coelho MA,Mota M,Belo I

    更新日期:2008-02-01 00:00:00

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    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Tkachenko A,Da Silva L,Hearne J,Parveen S,Waguespack Y

    更新日期:2013-01-01 00:00:00

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    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Liu CQ,Chen QH,Tang B,Ruan H,He GQ

    更新日期:2007-08-01 00:00:00