Pyrimidine synthesis in Burkholderia cepacia ATCC 25416.

Abstract:

:Pyrimidine synthesis in the food spoilage agent Burkholderia cepacia ATCC 25416 was investigated. The five de novo pathway enzymes of pyrimidine biosynthesis were found to be active in B. cepacia ATCC 25416 and growth of this strain on uracil had an effect on the de novo enzyme activities. The in vitro regulation of aspartate transcarbamoylase activity in B. cepacia ATCC 25416 was studies and its activity was inhibited by PP(i), ATP, GTP, CTP and UTP. The enzymes cytidine deaminase, uridine phosphorylase and cytosine deaminase were found to be active in the salvage of pyrimidines in ATCC 25416. Overall, de novo pyrimidine synthesis in B. cepacia ATCC 25416 was regulated at the level of enzyme activity and its pyrimidine salvage enzymes differed from those found in B. cepacia ATCC 17759.

journal_name

Lett Appl Microbiol

authors

Li K,West TP

doi

10.1111/j.1472-765x.1995.tb01075.x

subject

Has Abstract

pub_date

1995-11-01 00:00:00

pages

340-3

issue

5

eissn

0266-8254

issn

1472-765X

journal_volume

21

pub_type

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