Abstract：
AIMS:Real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on a TaqMan-minor groove binder (MGB) probe was developed for the rapid detection of avian influenza virus subtype H5. METHODS AND RESULTS:Conserved regions in the haemagglutinin genes of avian influenza viruses subtype H5 served as targets for the primers and TaqMan-MGB probe design. Concentrations of primers and probe were optimized to improve the sensitivity and specificity of the reactions. A plasmid containing the haemagglutinin gene was constructed and in vitro transcribed for a quantitative assay of copy numbers of the target gene. The results revealed that the optimal concentration of primers and probe was 640 and 480 nmol l(-1), respectively. The threshold of 100 copies of target molecules could be detected. The linear range for detection was determined as 10(2) to 10(8) molecules in reaction. CONCLUSIONS:It took less than 3 h to complete the detection from viral RNA extraction, with good sensitivity and repeatability. SIGNIFICANCE AND IMPACT OF THE STUDY:Real-time RT-PCR assay with MGB probe was an effective means for quick and quantitative laboratory detection and monitoring of H5 avian influenza viruses.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Lu YY,Yan JY,Feng Y,Xu CP,Shi W,Mao HYdoi
10.1111/j.1472-765X.2007.02253.xsubject
Has Abstractpub_date
2008-01-01 00:00:00pages
20-5issue
1eissn
0266-8254issn
1472-765Xpii
LAM2253journal_volume
46pub_type
杂志文章abstract：AIMS:To examine the efficacy of liposome oral administration to induce systemic and mucosal immune responses against verotoxin-producing Escherichia coli (VTEC) and the effect of the induced antibodies on the binding of the bacteria to Caco-2 cells. METHODS AND RESULTS:Mice were immunized orally with VTEC antigen and ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2003.01278.x
更新日期：2003-01-01 00:00:00
abstract：:The ability of Stenotrophomonas maltophilia strain VUN 10,003 to degrade and detoxify high molecular weight polycyclic aromatic hydrocarbons (PAHs) was evaluated in a basal liquid medium. Using high cell density inocula of strain VUN 10,003, the concentration of pyrene, fluoranthene, benz[a]anthracene, benzo[a]pyrene,...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2000.00733.x
更新日期：2000-05-01 00:00:00
abstract：:Currently used alum precipitated and oil adjuvant vaccines against HS caused by Pasteurella multocida B:2, have side effects and short-lived immunity, leading to regular catastrophic outbreaks in bovines in Asian subcontinent. The need for the development of an improved vaccine with longer immunity and the ability to ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.13010
更新日期：2018-09-01 00:00:00
abstract：UNLABELLED:The influence of oenological factors on cold-active pectinases from 15 preselected indigenous yeasts belonging to Aureobasidium pullulans, Filobasidium capsuligenum, Rhodotorula dairenensis, Cryptococcus saitoi and Saccharomyces cerevisiae was investigated. Pectinolytic enzymes were constitutive or partially...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12390
更新日期：2015-05-01 00:00:00
abstract：:Irreversible electroporation (IRE) damages cell membranes and is used in medicine for nonthermal ablation of malignant tumours. Our aim was to evaluate the antimicrobial effect of IRE. The pathogenic micro-organisms, Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa and Candida al...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12996
更新日期：2018-07-01 00:00:00
abstract：AIMS:The objective of the present study was to determine the optimal culture conditions for exo-biopolymer production by Cordyceps militaris in shake flask culture. METHODS AND RESULTS:The optimal temperature and initial pH for both mycelial growth and exo-biopolymer production by Cordyceps militaris in shake flask cu...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2001.00950.x
更新日期：2001-07-01 00:00:00
abstract：AIMS:Haloarchaeal proteases function optimally in high salt (low water activity); thus, they offer an advantage over the nonhalophilic counterparts as biocatalysts for protease-catalysed peptide synthesis. The haloalkaliphilic archaeon Natrialba magadii secretes a solvent-tolerant protease, Nep (Natrialba magadii extra...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2010.02955.x
更新日期：2010-12-01 00:00:00
abstract：AIMS:To identify the abattoir source(s) of psychrophilic clostridia causing 'blown pack' spoilage of vacuum-packed chilled meats. METHODS AND RESULTS:Molecular procedures were used to detect the presence of specific 16S rRNA gene fragments of blown pack-causing clostridia in samples collected from a commercial abattoi...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2003.01332.x
更新日期：2003-01-01 00:00:00
abstract：AIMS:Evaluation of the activity profiles of standard prokaryotic translation inhibitors with different physicochemical properties under whole-cell and cell-free conditions. METHODS AND RESULTS:The minimal inhibitory concentration values (cell-free/whole-cell microg ml(-1)) for three aminoglycosides (neomycin, 0.01/6.9...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2007.02281.x
更新日期：2008-02-01 00:00:00
abstract：:The hydrogen peroxide (H2O2) stress response in Enterococcus faecalis ATCC19433 was investigated. A 2.4 mmol l-1 H2O2 pretreatment conferred protection against a lethal concentration (45 mmol l-1) of this agent. The relatively high concentrations of H2O2 used for adaptation and challenge treatments in Ent. faecalis em...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.1998.00325.x
更新日期：1998-04-01 00:00:00
abstract：AIMS:The aim of this study was to explore the occurrence of methicillin-resistant staphylococci in a large urban public transport system. METHODS AND RESULTS:Samples were taken from hand rails, which passengers hold onto when they are standing. In total, 1400 swabs taken from 55 vehicles (trolleybuses, trams and buses...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.2008.02436.x
更新日期：2008-10-01 00:00:00
abstract：AIMS:To compare a range of enrichment broths and enrichment temperatures for the isolation of Escherichia coli O157 by immunomagnetic separation (IMS) from sandy, loam and clay soils. METHODS AND RESULTS:Soils were spiked with cocktails of four atoxigenic strains of E. coli O157 and four strains of commensal E. coli. ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2002.01098.x
更新日期：2002-01-01 00:00:00
abstract：AIMS:The objective of this study was to identify compounds responsible for medicinal off-flavours produced by different species and strains of Alicyclobacillus in orange juice using a combination of chromatographic-coupled olfactometric techniques and gas chromatography-mass spectrometry (GC-MS). METHODS AND RESULTS:E...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2004.01636.x
更新日期：2005-01-01 00:00:00
abstract：AIMS:To investigate the incidence of Campylobacter and Salmonella contamination associated with supermarket and butchers' shop chicken and related packaging. METHOD AND RESULTS:Three hundred raw samples (whole chicken, chicken breast with skin or chicken pieces) were purchased on a monthly basis for seven months. Pack...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2001.01031.x
更新日期：2001-12-01 00:00:00
abstract：:The contribution of trehalose and hsp104 to barotolerance in Saccharomyces cerevisiae has been investigated. Mutant strains, which lacked the ability to accumulate trehalose and/or hsp104, were examined for barotolerance and thermotolerance. All the mutants showed lower barotolerance and thermotolerance than their con...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.1997.t01-1-00069.x
更新日期：1997-07-01 00:00:00
abstract：AIM:To estimate the prevalence of thermotolerant Campylobacter spp. in commercially reared partridges (Perdix perdix) in southern Italy. METHODS AND RESULTS:Cloacal swabs of partridges (n = 240), equally distributed between male and female birds, from a game bird farm located in the Southern Italy were examined for th...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2009.02667.x
更新日期：2009-09-01 00:00:00
abstract：AIMS:To examine the ability of Agrobacterium to attach to Metarhizium anisopliae var. acridum strain CG423 under co-cultivation and to develop an Agrobacterium-mediated method of gene delivery into strain CG423, a promising agent for biological control of grasshoppers. METHODS AND RESULTS:The co-cultivation of Agrobac...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2006.02092.x
更新日期：2007-03-01 00:00:00
abstract：AIMS:To propose a universal workflow of sample preparation method for the identification of highly pathogenic bacteria by MALDI-TOF MS. METHODS AND RESULTS:Fifteen bacterial species, including highly virulent Gram-positive (Bacillus anthracis and Clostridium botulinum) and Gram-negative bacteria (Brucella melitensis, ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2012.03255.x
更新日期：2012-07-01 00:00:00
abstract：AIMS:This study sought to evaluate the performance of two chromogenic media designed for the isolation of vancomycin-resistant enterococci (VRE) and compare them with a traditional bile-esculin medium for the isolation of VRE from stool samples. METHODS AND RESULTS:A total of 285 stool samples were inoculated onto Chr...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2008.02517.x
更新日期：2009-02-01 00:00:00
abstract：AIMS:To determine the effect of cold storage on the survival of Erwinia amylovora. METHODS AND RESULTS:The survival of E. amylovora was assessed during storage at 2 degrees C. Populations of E. amylovora inoculated into phosphate-buffered saline remained static, whereas in nutrient media populations increased at low t...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2003.01407.x
更新日期：2003-01-01 00:00:00
abstract：:Environmental air sampling was evaluated as a method to detect the presence of M. bovis in the vicinity of infected badgers and their setts. Airborne particles were collected on gelatine filters using a commercially available air sampling instrument and tested for the presence of M. bovis using bacteriological culture...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12055
更新日期：2013-05-01 00:00:00
abstract：AIMS:To provide comparative genome sequence data for two related model strains of Staphylococcus aureus (SH1000 and 8325-4) that are used extensively in laboratory research. METHODS AND RESULTS:Comparative genome sequencing was used to identify genetic differences between Staph. aureus SH1000 and the fully genome-sequ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2010.02885.x
更新日期：2010-09-01 00:00:00
abstract：:The present study demonstrates the siderophore production of two strains of Pseudomonas fluorescens and two of Ps. chlororaphis. The antimicrobial activities of these strains were studied against Salmonella typhimurium, Staphylococcus aureus, Fusarium culmorum, F. oxysporum and Aspergillus niger. Despite equal siderop...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.1996.tb01146.x
更新日期：1996-03-01 00:00:00
abstract：AIMS:Saccharomyces cerevisiae was used for studying the physiological effects of hydrostatic pressure. METHODS AND RESULTS:The effects of hydrostatic pressure on the ultrastructure of wild-type and trehalose-6-phosphate synthase (tps1) mutant cells were investigated by transmission electron microscopy. Pressure induce...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2001.00853.x
更新日期：2001-01-01 00:00:00
abstract：AIMS:To examine changes in membrane fatty acid profile attributed to the physiological adaptation of Lactobacillus helveticus during vacuum drying. METHODS AND RESULTS:The viability and membrane integrity of the cells after vacuum drying were measured by plate counts and DNA fluorescence dyes. The physiological adapta...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2009.02703.x
更新日期：2009-10-01 00:00:00
abstract：AIMS:This study assessed the plant growth-promoting ability of the bacterial strains Ochrobactrum intermedium (isolate CrT-1) and Bacillus cereus (isolate S-6). METHODS AND RESULTS:Two chromium resistant bacterial strains isolated from chromium-contaminated wastewater and soils were identified as O. intermedium CrT-1 ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2006.01977.x
更新日期：2006-10-01 00:00:00
abstract：AIMS:The current study examined the antimicrobial susceptibility of 86 Listeria spp. isolated from processed bison carcasses. MATERIALS AND METHODS:Susceptibility to 25 antimicrobial agents was determined using E-test and National Antimicrobial Resistance Monitoring System (NARMS) panels. Most Listeria isolates (88-98...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2006.02027.x
更新日期：2007-01-01 00:00:00
abstract：AIMS:The purpose of this study was to determine if DNA polymorphisms generated by RAPD-PCR could be used to characterize Group B streptococci (GBS) for epidemiological purposes. METHODS AND RESULTS:30 unrelated, previously serotyped strains were analysed by RAPD-PCR using two 10-mer primers (5' TGCGAGAGTC 3' and 5' AG...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2002.01177.x
更新日期：2002-01-01 00:00:00
abstract：AIMS:To explore the effect of Lactobacillus on redox state of colon chyme. METHODS AND RESULTS:Nine Lactobacillus strains were studied for the inhibition of lipid peroxide formation in Fe(2+)/ascorbate system and for their ability to chelate 'free' ferrous ion. The result shows both properties were strain specific and...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2009.02786.x
更新日期：2010-03-01 00:00:00
abstract：UNLABELLED:Cryptococcosis, a fungal infection that affects both immunocompromised and immunocompetent individuals, contributes to increasing indices of mortality and morbidity. The development of resistance by Cryptococcus spp., the limited number of commercial antifungal drugs and the various side effects of these dru...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12086
更新日期：2013-08-01 00:00:00
