Evaluation of a method to detect Mycobacterium bovis in air samples from infected Eurasian badgers (Meles meles) and their setts.

Abstract:

:Environmental air sampling was evaluated as a method to detect the presence of M. bovis in the vicinity of infected badgers and their setts. Airborne particles were collected on gelatine filters using a commercially available air sampling instrument and tested for the presence of M. bovis using bacteriological culture and real-time PCR. The sensitivity of bacteriological culture was broadly similar to that of real-time PCR when testing samples artificially spiked with M. bovis. Sampling was undertaken from directly under the muzzles of badgers which had been experimentally infected with M. bovis (37 samples), within enclosures housing the experimentally infected animals (50 samples), and in the vicinity of setts with resident infected wild badgers (52 samples). The methods employed did not detect M. bovis from either infected badgers or artificial or natural setts known to contain infected animals. However, samples taken at four of the six natural setts were positive for Mycobacterium gordonae.

journal_name

Lett Appl Microbiol

authors

Jones RM,Ashford R,Cork J,Palmer S,Wood E,Spyvee P,Parks S,Bennett A,Brewer J,Delahay R,Chambers M,Sawyer J

doi

10.1111/lam.12055

subject

Has Abstract

pub_date

2013-05-01 00:00:00

pages

361-5

issue

5

eissn

0266-8254

issn

1472-765X

journal_volume

56

pub_type

杂志文章
  • A RAPD-PCR genotyping assay which correlates with serotypes of group B streptococci.

    abstract:AIMS:The purpose of this study was to determine if DNA polymorphisms generated by RAPD-PCR could be used to characterize Group B streptococci (GBS) for epidemiological purposes. METHODS AND RESULTS:30 unrelated, previously serotyped strains were analysed by RAPD-PCR using two 10-mer primers (5' TGCGAGAGTC 3' and 5' AG...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01177.x

    authors: Zhang GW,Kotiw M,Daggard G

    更新日期:2002-01-01 00:00:00

  • Adaptation of Listeria monocytogenes in a simulated cheese medium: effects on virulence using the Galleria mellonella infection model.

    abstract:UNLABELLED:The aim of this study was to evaluate the effect of the acid and salt adaptation in a cheese-based medium on the virulence potential of Listeria monocytogenes strains isolated from cheese and dairy processing environment using the Galleria mellonella model. Four L. monocytogenes strains were exposed to a che...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12064

    authors: Schrama D,Helliwell N,Neto L,Faleiro ML

    更新日期:2013-06-01 00:00:00

  • Optimization of physical parameters for exo-biopolymer production in submerged mycelial cultures of two entomopathogenic fungi Paecilomyces japonica and Paecilomyces tenuipes.

    abstract:AIMS:In the present study, two different optimization techniques were used to determine the suitable operating parameters for exo-biopolymer production in submerged mycelial cultures of two entomopathogenic fungi Paecilomyces japonica and Paecilomyces tenuipes. METHODS AND RESULTS:First, the rotating simplex method, a...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.01884.x

    authors: Xu CP,Sinha J,Bae JT,Kim SW,Yun JW

    更新日期:2006-05-01 00:00:00

  • Effect of relative humidity on inactivation of foodborne pathogens using chlorine dioxide gas and its residues on tomatoes.

    abstract::The effect of relative humidity (RH) on the antimicrobial efficacy of chlorine dioxide (ClO2 ) gas against foodborne pathogens on tomatoes was evaluated. Also, levels of ClO2 residues on tomatoes after exposure to ClO2 gas under different RH conditions were measured to determine the quantity of solubilized ClO2 gas on...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13002

    authors: Park SH,Kim WJ,Kang DH

    更新日期:2018-08-01 00:00:00

  • Flurbiprofen, a unique non-steroidal anti-inflammatory drug with antimicrobial activity against Trichophyton, Microsporum and Epidermophyton species.

    abstract:AIMS:The objective of this study was to determine the antifungal activity of flurbiprofen against dermatophytes like Trichophyton, Microsporum and Epidermophyton species. METHODS AND RESULTS:Susceptibility tests were performed against dermatophytes like Trichophyton, Microsporum and Epidermophyton species by the micro...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01370.x

    authors: Chowdhury B,Adak M,Bose SK

    更新日期:2003-01-01 00:00:00

  • Evidence of the production of silver nanoparticles via pretreatment of Phoma sp.3.2883 with silver nitrate.

    abstract:AIMS:The objective was to demonstrate the size of silver particles produced by the filamentous fungus Phoma sp.3.2883 via adsorption and accumulation, and to confirm that this silver was in a reduced state. METHODS AND RESULTS:Mycelium was freeze-dried and then shake-cultured in a silver nitrate solution. It was found...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01348.x

    authors: Chen JC,Lin ZH,Ma XX

    更新日期:2003-01-01 00:00:00

  • Characterization of beta-lactamase and biofilm producing Enterobacteriaceae isolated from organized and backyard farm ducks.

    abstract::This study was undertaken to detect the occurrence of beta-lactamase and biofilm producing Enterobacteriaceae in healthy ducks. A total 202 cloacal swabs were collected from ducks kept in organized (n = 92) and backyard (n = 110) farms in West Bengal (India). The ducks had no history of antibiotic intake. Among the 87...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13170

    authors: Banerjee A,Bardhan R,Chowdhury M,Joardar SN,Isore DP,Batabyal K,Dey S,Sar TK,Bandyopadhyay S,Dutta TK,Samanta I

    更新日期:2019-08-01 00:00:00

  • Specific molecular detection of Carnobacterium piscicola SF668 in cold smoked salmon.

    abstract:AIMS:To establish a rapid and reliable multiplex PCR (mPCR)-based method allowing specific identification of Carnobacterium piscicola SF668 during storage of cold smoked salmon (CSS). METHODS AND RESULTS:CSS was inoculated with C. piscicola SF668 and stored at 4 degrees C. Samples were withdrawn at regular time interv...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2005.01696.x

    authors: Pellé E,Dousset X,Prévost H,Drider D

    更新日期:2005-01-01 00:00:00

  • Decontamination of dental unit water systems with hydrogen peroxide.

    abstract:AIMS:Transmission of microbial pathogens to patients from water in dental units is a concern. To reduce this risk, the decontaminating efficiency of hydrogen peroxide was evaluated. METHODS AND RESULTS:Three percent hydrogen peroxide diluted 1 : 4 in distilled water (contact time 15 min) was used daily to disinfect th...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01378.x

    authors: Zanetti F,De Luca G,Tarlazzi P,Stampi S

    更新日期:2003-01-01 00:00:00

  • Surfactant concentration and type affects the removal of Escherichia coli from pig skin during a simulated hand wash.

    abstract::The effect of surfactant type and concentration on a bland soap formulation's ability to remove bacteria from hands remains largely unstudied. Several combinations of surfactants and water were combined to test bacterial removal efficacy using a hand-washing device (two pieces of pig skin and a mechanical motor) to si...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12777

    authors: Jensen DA,Rogers MA,Schaffner DW

    更新日期:2017-10-01 00:00:00

  • Anti-MRSA activity of sophoraflavanone G and synergism with other antibacterial agents.

    abstract::Anti-MRSA activity of sophoraflavanone G (SFG) and synergism between SFG and antibacterial agents against MRSA (methicillin-resistant Staphylococcus aureus) were evaluated by means of Minimal Inhibitory Concentrations (MIC). The MICs of SFG against 27 strains of MRSA ranged from 3.13 to 6.25 micrograms ml-1. Synergism...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1998.00386.x

    authors: Sakagami Y,Mimura M,Kajimura K,Yokoyama H,Linuma M,Tanaka T,Ohyama M

    更新日期:1998-08-01 00:00:00

  • Faecal contamination of echinoderms: first report of heavy Escherichia coli loading of sea urchins from a natural growing area.

    abstract:UNLABELLED:Although little evidence existed to support that view, European countries and in particular France, have regarded echinoderms, including sea urchins, as low risk in terms of feacal contamination. It is hypothesized that the sea urchins mode of feeding, which is based on grazing and differs from bivalve mollu...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12524

    authors: Bouchoucha M,Piquet JC,Chavanon F,Dufresne C,Le Guyader FS

    更新日期:2016-02-01 00:00:00

  • Lactobacilli prevent hydroxy radical production and inhibit Escherichia coli and Enterococcus growth in system mimicking colon fermentation.

    abstract:AIMS:To explore the effect of Lactobacillus on redox state of colon chyme. METHODS AND RESULTS:Nine Lactobacillus strains were studied for the inhibition of lipid peroxide formation in Fe(2+)/ascorbate system and for their ability to chelate 'free' ferrous ion. The result shows both properties were strain specific and...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2009.02786.x

    authors: Sun J,Hu XL,Le GW,Shi YH

    更新日期:2010-03-01 00:00:00

  • The oxidative stress response in Enterococcus faecalis: relationship between H2O2 tolerance and H2O2 stress proteins.

    abstract::The hydrogen peroxide (H2O2) stress response in Enterococcus faecalis ATCC19433 was investigated. A 2.4 mmol l-1 H2O2 pretreatment conferred protection against a lethal concentration (45 mmol l-1) of this agent. The relatively high concentrations of H2O2 used for adaptation and challenge treatments in Ent. faecalis em...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1998.00325.x

    authors: Flahaut S,Laplace JM,Frère J,Auffray Y

    更新日期:1998-04-01 00:00:00

  • Clinically applicable irreversible electroporation for eradication of micro-organisms.

    abstract::Irreversible electroporation (IRE) damages cell membranes and is used in medicine for nonthermal ablation of malignant tumours. Our aim was to evaluate the antimicrobial effect of IRE. The pathogenic micro-organisms, Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa and Candida al...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12996

    authors: Korem M,Goldberg NS,Cahan A,Cohen MJ,Nissenbaum I,Moses AE

    更新日期:2018-07-01 00:00:00

  • Rapid purification of Ti plasmids from Agrobacterium by ethidium bromide treatment and phenol extraction.

    abstract::An efficient method is described for the purification of Ti plasmid DNA from Agrobacterium. The procedure is based on the relative binding capacity of ethidium bromide to supercoiled plasmid DNA and linear DNA and on the high solubility of ethidium bromide in phenol. Following treatment with ethidium bromide, more tha...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1993.tb01415.x

    authors: Zhang L,Kerr A

    更新日期:1993-05-01 00:00:00

  • Citric acid production by Aspergillus niger on wet corn distillers grains.

    abstract:AIMS:To determine which citric acid-producing strain of Aspergillus niger utilized wet corn distillers grains most effectively to produce citric acid. METHODS AND RESULTS:Citric acid and biomass production by the fungal strains were analysed on the untreated grains or autoclaved grains using an enzyme assay and a grav...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.01958.x

    authors: Xie G,West TP

    更新日期:2006-09-01 00:00:00

  • Molecular characterization of Vibrio cholerae isolates from Iran 2012 and 2013 outbreaks.

    abstract:UNLABELLED:The aim of this study was to assess the genetic diversity of Vibrio cholerae isolated from 2012 and 2013 outbreaks in Iran, with regard to their virulence properties. A total of 20 V. cholerae strains were collected from Sistan-Baluchestan province of Iran during 2012 and 2013 outbreaks. Hybridization assays...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12571

    authors: Bakhshi B

    更新日期:2016-06-01 00:00:00

  • Effect of Lactobacillus isolates on the adhesion of pathogens to chicken intestinal mucus in vitro.

    abstract:AIMS:The aims of this study were to investigate in vitro the effects of Lactobacillus isolates from a chicken on adhesion of pathogenic Salmonella and Escherichia coli to chicken intestinal mucus obtained from different intestinal regions. METHODS AND RESULTS:Bacteria were labelled by using methyl-1,2-[(3)H]-thymidine...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.01844.x

    authors: Ma YL,Guo T,Xu ZR,You P,Ma JF

    更新日期:2006-04-01 00:00:00

  • Public transport as a reservoir of methicillin-resistant staphylococci.

    abstract:AIMS:The aim of this study was to explore the occurrence of methicillin-resistant staphylococci in a large urban public transport system. METHODS AND RESULTS:Samples were taken from hand rails, which passengers hold onto when they are standing. In total, 1400 swabs taken from 55 vehicles (trolleybuses, trams and buses...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.2008.02436.x

    authors: Stepanović S,Cirković I,Djukić S,Vuković D,Svabić-Vlahović M

    更新日期:2008-10-01 00:00:00

  • Antimicrobial susceptibilities of enterococci isolated from faeces of broiler and layer chickens.

    abstract::Enterococci were isolated from faecal droppings of chickens in broiler and layer farms and the susceptibilities to nine therapeutic antimicrobial agents and six growth-promoting antibiotics were determined by the agar dilution method. Resistance to therapeutic antimicrobial agents such as ampicillin, clindamycin, eryt...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.00842.x

    authors: Yoshimura H,Ishimaru M,Endoh YS,Kojima A

    更新日期:2000-12-01 00:00:00

  • Use of a rapid bioluminescence assay for detecting cyanobacterial microcystin toxicity.

    abstract::The recent rise in the awareness of the occurrence of toxic cyanobacterial blooms in aquatic environments, with associated human health problems and animal deaths, has increased the need for rapid, reliable and sensitive methods of determining cyanobacterial toxicity. A luminescent bacterial toxicity test was assessed...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1990.tb00161.x

    authors: Lawton LA,Campbell DL,Beattie KA,Codd GA

    更新日期:1990-10-01 00:00:00

  • Salmonella enteritidis and aerobic mesophiles in inoculated poultry sausages manufactured with high-pressure processing.

    abstract::Salmonella enteritidis-inoculated poultry sausages were pressurized at 500 MPa by combining different times (10 and 30 min) and temperatures (50, 60 and 70 degrees C) or heat treated with the same temperature-time combinations and a standard cooking (75 degrees C for 30 min). Counts of Salm. enteritidis and mesophilic...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2000.00829.x

    authors: Yuste J,Pla R,Mor-Mur M

    更新日期:2000-11-01 00:00:00

  • Improved antimicrobial activity of nisin-incorporated polymer films by formulation change and addition of food grade chelator.

    abstract:AIMS:The following polymers were developed: polyethylene (PE), a PE and polyethylene oxide (70% PE and 30% PEO; PE + PEO) blend, PE and nisin (PE + nisin), PE, nisin, and EDTA (PE + nisin + EDTA), and PE + PEO with nisin (PE + PEO + nisin). METHODS AND RESULTS:Of the polymers tested, PE and PE + PEO did not exhibit an...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2001.01005.x

    authors: Cutter CN,Willett JL,Siragusa GR

    更新日期:2001-10-01 00:00:00

  • Effect of humidity and temperature on the survival of Listeria monocytogenes on surfaces.

    abstract::Listeria monocytogenes is a pathogenic bacterium, with human disease and infection linked to dairy products, seafood, ready-to-eat meat and raw & undercooked meats. Stainless steel is the most common food preparation surface and therefore, it is important to understand how food storage conditions such as surface mater...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12714

    authors: Redfern J,Verran J

    更新日期:2017-04-01 00:00:00

  • Antibacterial and antiviral evaluation of sulfonoquinovosyldiacylglyceride: a glycolipid isolated from Azadirachta indica leaves.

    abstract:UNLABELLED:Assessment of antibacterial as well as antiherpes virus activity of sulfonoquinovosyldiacylglyceride (SQDG), a glycolipid, isolated from the leaves of Azadirachta indica has been described. Antimicrobial activity was evaluated against Gram-positive, Gram-negative bacteria and herpes simplex virus. SQDG showe...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12174

    authors: Bharitkar YP,Bathini S,Ojha D,Ghosh S,Mukherjee H,Kuotsu K,Chattopadhyay D,Mondal NB

    更新日期:2014-02-01 00:00:00

  • Hydroxyaldimines as potent in vitro anticryptococcal agents.

    abstract:UNLABELLED:Cryptococcosis, a fungal infection that affects both immunocompromised and immunocompetent individuals, contributes to increasing indices of mortality and morbidity. The development of resistance by Cryptococcus spp., the limited number of commercial antifungal drugs and the various side effects of these dru...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12086

    authors: Magalhães TF,da Silva CM,de Fátima Â,da Silva DL,Modolo LV,Martins CV,Alves RB,Ruiz AL,Longato GB,de Carvalho JE,de Resende-Stoianoff MA

    更新日期:2013-08-01 00:00:00

  • Does calculation of the 95th percentile of microbiological results offer any advantage over percentage exceedence in determining compliance with bathing water quality standards?

    abstract:UNLABELLED:AIMIS: Draft WHO guidance is likely to suggest a calculated 95th percentile method, rather than percentage exceedence, in assessing compliance of bathing waters with microbiological standards. This study set out to determine whether this was an appropriate development. METHODS AND RESULTS:A series of Monte ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01081.x

    authors: Hunter PR

    更新日期:2002-01-01 00:00:00

  • The influence of different concentrations of melatonin on the cell surface hydrophobic characteristics of Neisseria meningitidis.

    abstract::The cell surface hydrophobicity of micro-organisms is a characteristic that has been associated with the colonization of mammalian epithelia and with their capacity to induce diseases. Melatonin is a hormone produced by the pineal gland that affects the immune response mechanism. This study investigated, as an express...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2000.00813.x

    authors: Uberos J,Molina A,Liébana J,Augustin MC,Muñoz A

    更新日期:2000-10-01 00:00:00

  • Prevalence and distribution of White Spot Syndrome Virus in cultured shrimp.

    abstract::White Spot Syndrome Virus (WSSV) is a dsDNA virus causing White Spot Syndrome Disease (WSSD) in shrimp with almost 100% morality rate within 3-10 days. In Bangladesh, WSSD is one of the major impediments of shrimp farming. This study first investigated the prevalence and distribution of WSSV in cultured shrimps of the...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12353

    authors: Hossain A,Nandi SP,Siddique MA,Sanyal SK,Sultana M,Hossain MA

    更新日期:2015-02-01 00:00:00