Abstract:
AIMS:To establish a rapid and reliable multiplex PCR (mPCR)-based method allowing specific identification of Carnobacterium piscicola SF668 during storage of cold smoked salmon (CSS). METHODS AND RESULTS:CSS was inoculated with C. piscicola SF668 and stored at 4 degrees C. Samples were withdrawn at regular time intervals and analysed by counting the number of viable cells. About 25-100% of colonies grown on Elliker plates were subjected to mPCR amplification. The results show that strains presumably identified as C. piscicola SF668 were predominant over the test period. CONCLUSIONS:mPCR is a powerful tool to study competitiveness of C. piscicola SF668, which inhibits the growth of Listeria monocytogenes. SIGNIFICANCE AND IMPACT OF THE STUDY:The present study demonstrates the importance of molecular methods in studying competitiveness of strains with potential food applications.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Pellé E,Dousset X,Prévost H,Drider Ddoi
10.1111/j.1472-765X.2005.01696.xsubject
Has Abstractpub_date
2005-01-01 00:00:00pages
364-8issue
5eissn
0266-8254issn
1472-765Xpii
LAM1696journal_volume
40pub_type
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01310.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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journal_title:Letters in applied microbiology
pub_type: 杂志文章,评审
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更新日期:2014-12-01 00:00:00