Specific molecular detection of Carnobacterium piscicola SF668 in cold smoked salmon.

Abstract:

AIMS:To establish a rapid and reliable multiplex PCR (mPCR)-based method allowing specific identification of Carnobacterium piscicola SF668 during storage of cold smoked salmon (CSS). METHODS AND RESULTS:CSS was inoculated with C. piscicola SF668 and stored at 4 degrees C. Samples were withdrawn at regular time intervals and analysed by counting the number of viable cells. About 25-100% of colonies grown on Elliker plates were subjected to mPCR amplification. The results show that strains presumably identified as C. piscicola SF668 were predominant over the test period. CONCLUSIONS:mPCR is a powerful tool to study competitiveness of C. piscicola SF668, which inhibits the growth of Listeria monocytogenes. SIGNIFICANCE AND IMPACT OF THE STUDY:The present study demonstrates the importance of molecular methods in studying competitiveness of strains with potential food applications.

journal_name

Lett Appl Microbiol

authors

Pellé E,Dousset X,Prévost H,Drider D

doi

10.1111/j.1472-765X.2005.01696.x

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

364-8

issue

5

eissn

0266-8254

issn

1472-765X

pii

LAM1696

journal_volume

40

pub_type

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