Simultaneous detection of Fusarium asiaticum and Fusarium graminearum in wheat seeds using a real-time PCR method.

Abstract:

AIMS:To develop a PCR-based method for quantitative detection of Fusarium asiaticum (Fa) and Fusarium graminearum (Fg) in wheat seeds. METHODS AND RESULTS:Based on the sequences of the cyp51A gene, two primer pairs FaF + FaR and FgF + FgR were developed for the species-specific detection of Fa and Fg, respectively. To simultaneously detect these two phylogenetic species, a pair of primers FgaF + FgaR was developed based on the first and the second introns of beta-tubulin gene. This primer pair amplified a 228-bp fragment only from Fa and Fg isolates, but not from 22 other Fusarium spp. and 13 other fungal species. A real-time PCR with this primer pair was able to quantify minute amounts of Fa and Fg DNA in wheat seeds rapidly. CONCLUSIONS:PCR primers designed based on the sequence of cyp51A or intron region of beta-tubulin gene could allow differentiation of genetically related fungal species. SIGNIFICANCE AND IMPACT OF THE STUDY:The sensitive and quantitative detection method can be readily used in epidemiological studies and in assessing risk of Fusarium mycotoxin contamination in wheat samples.

journal_name

Lett Appl Microbiol

authors

Yin Y,Liu X,Ma Z

doi

10.1111/j.1472-765X.2009.02595.x

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

680-6

issue

6

eissn

0266-8254

issn

1472-765X

pii

LAM2595

journal_volume

48

pub_type

杂志文章
  • Antagonists of bacitracin.

    abstract::Three groups of substances were identified which inhibit the bactericidal activity of bacitracin. Beside two divalent metals, Mg2+ and Ca2+, and a metabolite with chelating properties, citrate, the most pronounced effect was observed with pyrophosphate. Metals probably prevent access of bacitracin to the lipid carrier...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1994.tb00916.x

    authors: Podlesek Z,Comino A

    更新日期:1994-08-01 00:00:00

  • Evidence of lethal and sublethal injury in food-borne bacterial pathogens exposed to high-intensity pulsed-plasma gas discharges.

    abstract:AIMS:To apply scanning electron microscopy, image analysis and a fluorescent viability stain to assess lethal and sublethal injury in food-borne bacteria exposed to pulsed-plasma gas discharges (PPGD). METHODS AND RESULTS:The fluorescent redox probe 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) was used for enumerati...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02268.x

    authors: Rowan NJ,Espie S,Harrower J,Farrell H,Marsili L,Anderson JG,MacGregor SJ

    更新日期:2008-01-01 00:00:00

  • Pyrosequencing as a tool for the detection of Phytophthora species: error rate and risk of false Molecular Operational Taxonomic Units.

    abstract:AIMS:To evaluate the accuracy of pyrosequencing for the description of Phytophthora communities in terms of taxa identification and risk of assignment for false Molecular Operational Taxonomic Units (MOTUs). METHODS AND RESULTS:Pyrosequencing of Internal Transcribed Spacer 1 (ITS1) amplicons was used to describe the s...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.2012.03310.x

    authors: Vettraino AM,Bonants P,Tomassini A,Bruni N,Vannini A

    更新日期:2012-11-01 00:00:00

  • Evaluation of an alternative method for the enumeration and confirmation of Clostridium perfringens from treated and untreated sewages.

    abstract:AIMS:Clostridium perfringens is recommended as a suitable indicator bacterium for human enteric viruses, Giardia cysts and Cryptosporidium oocysts in finished water and in the assessment and evaluation of water treatment. Several agars and confirmation procedures were evaluated in parallel with the Australian/New Zeala...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.01912.x

    authors: Wohlsen T,Bayliss J,Gray B,Bates J,Katouli M

    更新日期:2006-05-01 00:00:00

  • The incidence and PCR detection of Campylobacter upsaliensis in dogs and cats.

    abstract::A study was made of the incidence of Campylobacter upsaliensis among dogs and cats suffering from acute or chronic diarrhoea; 225 dogs and cats were examined and 51 strains were identified, 16 (7%) of which were Camp. upsaliensis. When rectal swabs were taken from a control group of 126 dogs and cats without clinical ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.00799.x

    authors: Steinhauserova I,Fojtikova K,Klimes J

    更新日期:2000-09-01 00:00:00

  • Molasses supplementation promotes conidiation but suppresses aflatoxin production by small sclerotial Aspergillus flavus.

    abstract:AIMS:To find a supplemental ingredient that can be added to routinely used growth media to increase conidial production and decrease aflatoxin biosynthesis in small sclerotial (S strain) isolates of Aspergillus flavus. METHODS AND RESULTS:Molasses was added to three commonly used culture media: coconut agar (CAM), pot...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.02056.x

    authors: Chang PK,Hua SS

    更新日期:2007-02-01 00:00:00

  • Analysis of pristinamycin-resistant Staphylococcus epidermidis isolates in the Tunisian Bone Marrow Transplant Center.

    abstract:AIMS:We report the analysis of genetic determinants conferring resistance to pristinamycin in Staphylococcus epidermidis strains and epidemiology typing of these strains by pulsed-field gel electrophoresis. METHODS AND RESULTS:Staphylococcus epidermidis (346 isolates) were searched for strains with pristinamycin resis...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02318.x

    authors: Achour W,Bouchami O,Galopin S,Leclercq R,Ben Hassen A

    更新日期:2008-03-01 00:00:00

  • Hydrolytic properties of crude alpha-L-rhamnosidases produced by several wild strains of mesophilic fungi.

    abstract:AIMS:Observation of the dependence of alpha-L-rhamnosidase activity on pH and temperature and the capability to hydrolyse concentrated naringin solutions and hesperidin suspensions of enzyme complexes produced by several fungi. METHODS AND RESULTS:The enzymes were produced by several wild strains of mesophilic fungi g...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01115.x

    authors: Scaroni E,Cuevas C,Carrillo L,Ellenrieder G

    更新日期:2002-01-01 00:00:00

  • Flurbiprofen, a unique non-steroidal anti-inflammatory drug with antimicrobial activity against Trichophyton, Microsporum and Epidermophyton species.

    abstract:AIMS:The objective of this study was to determine the antifungal activity of flurbiprofen against dermatophytes like Trichophyton, Microsporum and Epidermophyton species. METHODS AND RESULTS:Susceptibility tests were performed against dermatophytes like Trichophyton, Microsporum and Epidermophyton species by the micro...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01370.x

    authors: Chowdhury B,Adak M,Bose SK

    更新日期:2003-01-01 00:00:00

  • Effect of copper hydroxide sprays for citrus canker control on wild-type Escherichia coli.

    abstract:AIMS:  To show that application of copper hydroxide citrus sprays mixed with field source water (possibly contaminated) will not support Escherichia coli on plant surfaces. Environmental stresses of transient phyllosphere bacteria and presence of copper will eradicate these bacteria before harvest. METHODS AND RESULTS...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2011.03179.x

    authors: Narciso JA,Ference CM,Ritenour MA,Widmer WW

    更新日期:2012-02-01 00:00:00

  • The effect of colony age on PCR fingerprinting.

    abstract::The pattern of DNA fragments produced by the polymerase chain reaction using arbitrary primers is strongly influenced by the age of the colonies sampled. We show that the number, clarity and reproducibility of the bands produced is optimal when very young colonies are sampled. ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1993.tb01467.x

    authors: Coutinho HL,Handley BA,Kay HE,Stevenson L,Beringer JE

    更新日期:1993-12-01 00:00:00

  • Pseudomonas fluorescens and closely-related fluorescent pseudomonads as biocontrol agents of soil-borne phytopathogens.

    abstract::Many strains of Pseudomonas fluorescens show potential for biological control of phytopathogens especially root pathogens. In taxonomic terms, several of them are indeed P. fluorescens sensu stricto, while others belong in fact to neighbouring species of the 'P. fluorescens' complex or to ill-defined related species w...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2009.02566.x

    authors: Couillerot O,Prigent-Combaret C,Caballero-Mellado J,Moënne-Loccoz Y

    更新日期:2009-05-01 00:00:00

  • Presence of sourdough lactic acid bacteria in commercial total mixed ration silage as revealed by denaturing gradient gel electrophoresis analysis.

    abstract:AIMS:To characterize the bacterial communities in commercial total mixed ration (TMR) silage, which is known to have a long bunk life after silo opening. METHODS AND RESULTS:Samples were collected from four factories that produce TMR silage according to their own recipes. Three factories were sampled three times at 1-...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2010.02915.x

    authors: Wang C,Nishino N

    更新日期:2010-10-01 00:00:00

  • Treatment with chlorous acid to inhibit spores of Alicyclobacillus acidoterrestris in aqueous suspension and on apples.

    abstract:AIM:To test the efficacy of a chemical (chlorous acid) for reducing the numbers of viable Alicyclobacillus acidoterrestris spores in laboratory media and on apples. METHODS AND RESULTS:Alicyclobacillus acidoterrestris spores in aqueous suspension and on apple surfaces of four different cultivars were treated with 268 ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2010.02874.x

    authors: Lee SY,Ryu SR,Kang DH

    更新日期:2010-08-01 00:00:00

  • Antimicrobial efficacy of cryotreatment against Enterococcus faecalis in root canals.

    abstract::The purpose of this investigation was to evaluate intracanal bacterial reduction by cryotreatment using a dental instrument equipped with a duct and connected to a cryogenic fluid source. A total of 86 roots were infected with Enterococcus faecalis and incubated. After incubation, the contaminated roots were divided i...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12017

    authors: Mandras N,Allizond V,Bianco A,Banche G,Roana J,Piazza L,Viale P,Cuffini AM

    更新日期:2013-02-01 00:00:00

  • Isolation and characterization of Clostridium difficile from a small survey of wastewater, food and animals in New Zealand.

    abstract::The objective of this study was to undertake a microbiological survey of foods, animal faeces and wastewater samples for Clostridium difficile, and determine the genotypes and antimicrobial susceptibilities of isolates. A total of 211 samples were tested for C. difficile using culture methods. Thirteen toxigenic C. di...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13238

    authors: Rivas L,Dupont PY,Gilpin BJ,Cornelius AJ

    更新日期:2020-01-01 00:00:00

  • Evaluation of a method to detect Mycobacterium bovis in air samples from infected Eurasian badgers (Meles meles) and their setts.

    abstract::Environmental air sampling was evaluated as a method to detect the presence of M. bovis in the vicinity of infected badgers and their setts. Airborne particles were collected on gelatine filters using a commercially available air sampling instrument and tested for the presence of M. bovis using bacteriological culture...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12055

    authors: Jones RM,Ashford R,Cork J,Palmer S,Wood E,Spyvee P,Parks S,Bennett A,Brewer J,Delahay R,Chambers M,Sawyer J

    更新日期:2013-05-01 00:00:00

  • Methylene blue active substances in plaque of Bacillus subtilis subsp. subtilis and enrichment by supplemental calcium in culture media.

    abstract::A series of experiments was conducted to identify the molecular species responsible for surface active emulsification (surfactant) bioactivity in Bacillus subtilis subsp. subtilis strain ATCC PTA-125135, and to describe culture conditions to support the enriched production of said bioactivity in cultured plaque of the...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13370

    authors: Krueger LA,Grzemski MA,Bilyeu MC,Horst JG,Ugrin SA,Hosette CA,Spangler DA,Ayangbile GA

    更新日期:2020-11-01 00:00:00

  • Selection of Salmonella typhimurium as an indicator for pathogen regrowth potential in composted biosolids.

    abstract::In order to select a suitable indicator for monitoring the pathogen regrowth potential of composted biosolids, the growth kinetics of selected bacteria were investigated. Growth parameters of six serovars of Salmonella and three strains of Escherichia coli in sterilized compost were compared. Seeded Salmonella and E. ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00626.x

    authors: Sidhu J,Gibbs RA,Ho GE,Unkovich I

    更新日期:1999-11-01 00:00:00

  • Combination of ARAD microfibre filtration and LAMP methodology for simple, rapid and cost-effective detection of human pathogenic Giardia duodenalis and Cryptosporidium spp. in drinking water.

    abstract:AIMS:In this study, we report a new, simple methodology for the monitoring of Cryptosporidium oocysts and Giardia cysts in drinking water samples, ranging from 10- to 1000-l, which combines a new ARAD microfibre filtration of the (oo)cysts from drinking water and loop-mediated isothermal amplification (LAMP) of a human...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2009.02758.x

    authors: Plutzer J,Törökné A,Karanis P

    更新日期:2010-01-01 00:00:00

  • Characterization of Bacillus thuringiensis serovar bolivia (serotype H63), a novel serovar isolated from the Bolivian high valleys.

    abstract::The type strain Bacillus thuringiensis var. bolivia (serotype H63), isolated from the Bolivian high valleys, has been characterized at different levels. Its parasporal crystal has an unusual shape and it is composed of a protein of 155 kDa which shows two bands of 75 and 80 kDa after activation. Analysis by PCR shows ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00558.x

    authors: Ferrandis MD,Andrew R,Porcar M,Iriarte J,Cosmao-Dumanoir V,Lecadet MM,Caballero P,Ferré J

    更新日期:1999-06-01 00:00:00

  • Modelling the effects of lactic acid, sodium benzoate and temperature on the growth of Candida maltosa.

    abstract::The growth of the oxidatively imperfect yeast Candida maltosa Komagata, Nakase et Katsuya was studied experimentally and modelled mathematically in relation to sodium benzoate and lactic acid concentrations at different temperatures. Application of gamma models for the growth rate resulted in determination of cardinal...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12803

    authors: Valík Ľ,Ačai P,Liptáková D

    更新日期:2017-11-01 00:00:00

  • Enumeration of micro-organisms in processed soy products with an automated most probable number method compared with standard plate method.

    abstract:AIM:The automated TEMPO system (bioMerieux) is based on the most probable number (MPN) method for the enumeration of micro-organisms in foods. In this study, we evaluated the performance of the TEMPO system as a diagnostic tool in comparison with the standard method in processed soy products. METHODS AND RESULTS:A ver...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2011.03143.x

    authors: Katase M,Tsumura K

    更新日期:2011-11-01 00:00:00

  • Is a foetus developing in a sterile environment?

    abstract::Novel findings in microbiology question the long-standing paradigm that a healthy pregnancy implies a sterile uterus. It now seems that the placenta is frequently colonized with bacteria, and a placental microbiome has been identified. Recent literature findings are summarized here, and an attempt is made to separate ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/lam.12334

    authors: Wassenaar TM,Panigrahi P

    更新日期:2014-12-01 00:00:00

  • Detection and molecular characterization of Livestock-Associated MRSA in raw meat on retail sale in North West England.

    abstract::Limited data are available on the prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in the UK. We tested 124 raw meat samples for MRSA including pork (n = 63), chicken (n = 50) and turkey (n = 11) collected from retail outlets in North West England between March and July 2015. MR...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12709

    authors: Fox A,Pichon B,Wilkinson H,Doumith M,Hill RL,McLauchlin J,Kearns AM

    更新日期:2017-03-01 00:00:00

  • The host model Galleria mellonella is resistant to taylorellae infection.

    abstract::The genus Taylorella is composed of two species: (i) Taylorella equigenitalis, the causative agent of CEM, a venereally transmitted infection of Equidae and (ii) Taylorella asinigenitalis, a closely related species considered to be nonpathogenic, although experimental infection of mares with this bacterium resulted in...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12297

    authors: Hébert L,Rincé I,Sanna C,Laugier C,Rincé A,Petry S

    更新日期:2014-10-01 00:00:00

  • Prevalence of virulence and extended-spectrum β-lactamase (ESBL) genes harbouring Vibrio spp. isolated from cockles (Tegillarca granosa) marketed in Korea.

    abstract::The present study aimed to investigate the incidence, virulence and antibiotic properties in Vibrio spp. isolated from cockles (Tegillarca granosa) marketed in Korea. A total of 32 Vibrio spp. isolates including V. parahaemolyticus (n = 4), V. alginolyticus (n = 11), V. diabolicus (n = 14) and V. harveyi (n = 3) were ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13232

    authors: Dahanayake PS,Hossain S,Wickramanayake MVKS,Heo GJ

    更新日期:2020-07-01 00:00:00

  • Genetic heterogeneity of non-O1 and non-O139 Vibrio cholerae isolates from shrimp aquaculture system: a comparison of RS-, REP- and ERIC-PCR fingerprinting approaches.

    abstract:AIMS:The genetic diversity of Vibrio cholerae isolated from black tiger shrimp (Penaeus monodon) aquaculture farms was determined using three PCR typing methods based on enterobacterial repetitive intergenic consensus (ERIC) sequences, ribosomal gene spacer (RS) sequence and repetitive extragenic palindromic (REP) sequ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2010.02863.x

    authors: Rao BM,Surendran PK

    更新日期:2010-07-01 00:00:00

  • Diagnostic value of recombinant Tp0821 protein in serodiagnosis for syphilis.

    abstract:UNLABELLED:Syphilis is a multistage sexually transmitted disease that remains a serious public health concern worldwide. The coexistence of Treponema pallidum with other closely related members of spirochaeta, such as Leptospira spp. and Borrelia burgdorferi, has complicated the serodiagnosis due to cross-reactive anti...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12554

    authors: Xie Y,Xu M,Wang C,Xiao J,Xiao Y,Jiang C,You X,Zhao F,Zeng T,Liu S,Kuang X,Wu Y

    更新日期:2016-04-01 00:00:00

  • Physiological response of Enterococcus faecalis JH2-2 to cold shock: growth at low temperatures and freezing/thawing challenge.

    abstract::Growth at low positive temperatures and induced phenotypic resistance to extreme cold temperature (freezing/thawing cycles) of Enterococcus faecalis were investigated. The effect of low temperatures on the specific growth rates was studied; use of Arrhenius profile and Ratkovsky 'square-root' model allowed determinati...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1996.tb01345.x

    authors: Thammavongs B,Corroler D,Panoff JM,Auffray Y,Boutibonnes P

    更新日期:1996-12-01 00:00:00