Abstract:
AIMS:To develop a PCR-based method for quantitative detection of Fusarium asiaticum (Fa) and Fusarium graminearum (Fg) in wheat seeds. METHODS AND RESULTS:Based on the sequences of the cyp51A gene, two primer pairs FaF + FaR and FgF + FgR were developed for the species-specific detection of Fa and Fg, respectively. To simultaneously detect these two phylogenetic species, a pair of primers FgaF + FgaR was developed based on the first and the second introns of beta-tubulin gene. This primer pair amplified a 228-bp fragment only from Fa and Fg isolates, but not from 22 other Fusarium spp. and 13 other fungal species. A real-time PCR with this primer pair was able to quantify minute amounts of Fa and Fg DNA in wheat seeds rapidly. CONCLUSIONS:PCR primers designed based on the sequence of cyp51A or intron region of beta-tubulin gene could allow differentiation of genetically related fungal species. SIGNIFICANCE AND IMPACT OF THE STUDY:The sensitive and quantitative detection method can be readily used in epidemiological studies and in assessing risk of Fusarium mycotoxin contamination in wheat samples.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Yin Y,Liu X,Ma Zdoi
10.1111/j.1472-765X.2009.02595.xsubject
Has Abstractpub_date
2009-06-01 00:00:00pages
680-6issue
6eissn
0266-8254issn
1472-765Xpii
LAM2595journal_volume
48pub_type
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