Application of ARISA to assess the influence of salt content and cation type on microbiological diversity of Cheddar cheese.

abstract：UNLABELLED:Syphilis is a multistage sexually transmitted disease that remains a serious public health concern worldwide. The coexistence of Treponema pallidum with other closely related members of spirochaeta, such as Leptospira spp. and Borrelia burgdorferi, has complicated the serodiagnosis due to cross-reactive anti...

journal_title：Letters in applied microbiology

authors： Xie Y,Xu M,Wang C,Xiao J,Xiao Y,Jiang C,You X,Zhao F,Zeng T,Liu S,Kuang X,Wu Y

更新日期：2016-04-01 00:00:00

abstract：AIMS:To apply scanning electron microscopy, image analysis and a fluorescent viability stain to assess lethal and sublethal injury in food-borne bacteria exposed to pulsed-plasma gas discharges (PPGD). METHODS AND RESULTS:The fluorescent redox probe 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) was used for enumerati...

journal_title：Letters in applied microbiology

authors： Rowan NJ,Espie S,Harrower J,Farrell H,Marsili L,Anderson JG,MacGregor SJ

更新日期：2008-01-01 00:00:00

abstract：:A set of Escherichia coli freshwater isolates was chosen to compare the effectiveness of denaturing gradient gel electrophoresis (DGGE) vs temporal temperature gradient gel electrophoresis (TTGE) for separating homologous amplicons from the respective uidA region differing in one to seven single base substitutions. Bo...

journal_title：Letters in applied microbiology

authors： Farnleitner AH,Kreuzinger N,Kavka GG,Grillenberger S,Rath J,Mach RL

更新日期：2000-06-01 00:00:00

abstract：AIMS:The antigen 85 complex (Ag85) from Mycobacterium tuberculosis consists of three abundantly secreted proteins (FbpA, FbpB and FbpC2) which play a key role in the pathogenesis of tuberculosis and also exhibit cell wall mycolyltransferase activity. A related protein with similarity to the Ag85 complex was recently an...

journal_title：Letters in applied microbiology

authors： Kremer L,Maughan WN,Wilson RA,Dover LG,Besra GS

更新日期：2002-01-01 00:00:00

abstract：AIMS:The objective of this study was to subtype Arcobacter butzleri isolates using RAPD-PCR. METHODS AND RESULTS:Thirty-five A. butzleri isolates obtained from chicken carcasses were examined. PCR-mediated DNA fingerprinting technique with primers of the variable sequence motifs was used to detect polymorphism within ...

journal_title：Letters in applied microbiology

authors： Atabay HI,Bang DD,Aydin F,Erdogan HM,Madsen M

更新日期：2002-01-01 00:00:00

abstract：:A monoclonal antibody (mAb) has been produced to aflatoxin B1 (AF B1) after successful immunization of mice and fusion of sensitized spleen cells with myeloma cancer cells. The mice were immunized with AF B1-oxime-protein conjugate. Positive mAbs were screened using an indirect ELISA specific for AF B1. The selected m...

journal_title：Letters in applied microbiology

authors： Candlish AA,Smith JE,Stimson WH

更新日期：1990-04-01 00:00:00

abstract：:The yield of purified recombinant alpha-sarcin increases approximately three- to fourfold when this toxin is co-expressed in Escherichia coli with thioredoxin. This increased production is attributed to the existence, in the presence of thioredoxin, of a reducing environment which allows rearrangement of incorrect dis...

journal_title：Letters in applied microbiology

authors： García-Ortega L,Lacadena J,Lacadena V,Masip M,De Antonio C,Martínez-Ruiz A,Martínez Del Pozo A

更新日期：2000-04-01 00:00:00

abstract：AIMS:To investigate interactions, if any, between temperature, ferric ammonium citrate and glycine betaine on the growth of Listeria monocytogenes in modified Pine's medium (Pine et al. 1989). METHODS AND RESULTS:Modified Pine's medium containing 0, 0.044, 0.088 or 0.176 g l(-1) ferric ammonium citrate, and 0 or 1 mM ...

journal_title：Letters in applied microbiology

authors： Dykes GA,Dworaczek M

更新日期：2002-01-01 00:00:00

abstract：:This study evaluated the performance of CPS ID2 (bioMérieux) compared to that of conventionally used selective agar for the identification of bacteria responsible for urinary tract infections. This medium detects bacterial enzymes using chromogenic substrates. Two hundred and nineteen samples of urine were tested in o...

journal_title：Letters in applied microbiology

authors： Willinger B,Manafi M

更新日期：1995-05-01 00:00:00

abstract：AIMS:To assess the ability of staphylococcal bacteriophage K to inhibit Staphylococcus aureus in raw milk. METHODS AND RESULTS:The ability of bacteriophage (phage) to replicate in milk is important in situations where phage might be used as a therapeutic for bovine mastitis. Phage K was able to replicate normally, lea...

journal_title：Letters in applied microbiology

authors： O'Flaherty S,Coffey A,Meaney WJ,Fitzgerald GF,Ross RP

更新日期：2005-01-01 00:00:00

abstract：UNLABELLED:Most of the current research into the quantification of soil-borne pathogenic oomycetes lacks determination of DNA extraction efficiency, probably leading to an incorrect estimation of DNA quantity. In this study, we developed a convenient method by using a 100 bp artificially synthesized DNA sequence derive...

journal_title：Letters in applied microbiology

authors： Li M,Ishiguro Y,Kageyama K,Zhu Z

更新日期：2015-08-01 00:00:00

abstract：AIMS:The aim of the study was to determine Campylobacter jejuni contamination and prevalence on fomites moving between broiler farms and the processing plant in the period after cleaning and before departure to harvest chickens. In addition, changes in the proportion of contaminated fomites in the course of a day were ...

journal_title：Letters in applied microbiology

authors： Ramabu SS,Boxall NS,Madie P,Fenwick SG

更新日期：2004-01-01 00:00:00

abstract：:The metabolism of glucose was studied in Lactococcus lactis subsp. CNRZ 125 by 13C NMR. The initial rate of glucose utilization was higher for exponential phase cells than for stationary phase cells [150 vs 85 nmol g (dry wt)-1 s -1]. 31P NMR was used to determine changes in glycolytic phosphorylated intermediates (fr...

journal_title：Letters in applied microbiology

authors： Foucaud C,Herve M,Neumann JM,Hemme D

更新日期：1995-07-01 00:00:00

abstract：AIMS: To develop a specific and highly sensitive loop-mediated isothermal amplification (LAMP) technique for the rapid detection of canine parvovirus (CPV) DNA directly in suspected faecal samples of dogs by employing a simple method of template preparation. METHODS AND RESULTS: LAMP reaction was developed by designin...

journal_title：Letters in applied microbiology

authors： Mukhopadhyay HK,Amsaveni S,Matta SL,Antony PX,Thanislass J,Pillai RM

更新日期：2012-09-01 00:00:00

abstract：:Alzheimer's disease (AD) is the most common form of dementia and one of the major causes of disability and dependency in older people. Accumulating evidences link gut microbiota with different diseases and its relationship with neurodegenerative diseases is becoming most intriguing. This study was aimed to compare the...

journal_title：Letters in applied microbiology

authors： Bäuerl C,Collado MC,Diaz Cuevas A,Viña J,Pérez Martínez G

更新日期：2018-06-01 00:00:00

abstract：:While patients with cystic fibrosis (CF) have had dramatic improvement in their survival rates, this has been accompanied by the emergence of more virulent pathogens such as Pseudomonas aeruginosa and Burkholderia cepacia complex organisms. In addition, there has been emergence of organisms of increasing clinical sign...

journal_title：Letters in applied microbiology

authors： Jordan PW,Stanley T,Donnelly FM,Elborn JS,McClurg RB,Millar BC,Goldsmith CE,Moore JE

更新日期：2007-05-01 00:00:00

abstract：AIMS:The aim of this study was to explore the occurrence of methicillin-resistant staphylococci in a large urban public transport system. METHODS AND RESULTS:Samples were taken from hand rails, which passengers hold onto when they are standing. In total, 1400 swabs taken from 55 vehicles (trolleybuses, trams and buses...

journal_title：Letters in applied microbiology

authors： Stepanović S,Cirković I,Djukić S,Vuković D,Svabić-Vlahović M

更新日期：2008-10-01 00:00:00

abstract：AIMS:This study evaluated the inactivation of virulent Yersinia pestis dried on polymers, steel, and glass surfaces using vapour-phase hydrogen peroxide. METHODS AND RESULTS:A suspension of Y. pestis CO92 (1.70 x 10(8) CFU) was dried on 10 different types of test surfaces and exposed to vapour-phase hydrogen peroxide ...

journal_title：Letters in applied microbiology

authors： Rogers JV,Richter WR,Shaw MQ,Choi YW

更新日期：2008-10-01 00:00:00

abstract：AIMS:To evaluate the effect of protective agents upon survival of Lactobacillus delbrueckii ssp. bulgaricus during freeze-drying and storage, and selective amino acids on cell membrane fluidity. METHODS AND RESULTS:The protective effect of amino-acids and sugars at different concentrations was studied by determining t...

journal_title：Letters in applied microbiology

authors： Martos GI,Minahk CJ,de Valdez GF,Morero R

更新日期：2007-09-01 00:00:00

abstract：:In a crossover study, volunteers consumed a diet supplemented with either wheat or rye bread. Levels of faecal mucus were determined and the adhesion of five selected probiotics to immobilized intestinal mucus, was assessed. No significant difference in the concentration of faecal mucus or adhesion of the probiotics t...

journal_title：Letters in applied microbiology

authors： Ouwehand AC,Gråsten S,Niemi P,Mykkänen H,Salminen S

更新日期：2000-07-01 00:00:00

abstract：:Cow's milk was inoculated with ca 10(3) and 10(7) cfu ml-1 Escherichia coli O157:H7. After fermentation at 42 degrees C for 0-5 h, the yoghurt was stored at 4 degrees C. Two kinds of yoghurt were used: traditional yoghurt (TY), made with Streptococcus thermophilus and Lactobacillus bulgaricus starter cultures, and 'bi...

journal_title：Letters in applied microbiology

authors： Massa S,Altieri C,Quaranta V,De Pace R

更新日期：1997-05-01 00:00:00

abstract：:The marine antimicrobial peptide NZ17074, a variant of arenicin-3 from Arenicola marina that has broad antimicrobial activity and high bioavailability, can be designed to treat bacterial and fungal diseases. To reduce the toxicity of NZ17074, N6 was designed by replacing a cysteine in positions 3 and 20 with alanine, ...

journal_title：Letters in applied microbiology

authors： Yang N,Wang X,Teng D,Mao R,Hao Y,Feng X,Wang J

更新日期：2017-09-01 00:00:00

abstract：:Shigella spp. are water-borne pathogens responsible for mild to severe cases bacilli dysentery all around the world known as Shigellosis. The progressively increasing of antibiotic resistance among Shigella calls for developing and establishing novel alternative therapeutic methods. The present study aimed to evaluate...

journal_title：Letters in applied microbiology

authors： Shahin K,Zhang L,Bao H,Hedayatkhah A,Soleimani-Delfan A,Komijani M,He T,Barazandeh M,Mansoorianfar M,Bouzari M,Wang R

更新日期：2020-10-18 00:00:00

abstract：AIMS:Clostridium perfringens is recommended as a suitable indicator bacterium for human enteric viruses, Giardia cysts and Cryptosporidium oocysts in finished water and in the assessment and evaluation of water treatment. Several agars and confirmation procedures were evaluated in parallel with the Australian/New Zeala...

journal_title：Letters in applied microbiology

authors： Wohlsen T,Bayliss J,Gray B,Bates J,Katouli M

更新日期：2006-05-01 00:00:00

abstract：:Many strains of Pseudomonas fluorescens show potential for biological control of phytopathogens especially root pathogens. In taxonomic terms, several of them are indeed P. fluorescens sensu stricto, while others belong in fact to neighbouring species of the 'P. fluorescens' complex or to ill-defined related species w...

journal_title：Letters in applied microbiology

authors： Couillerot O,Prigent-Combaret C,Caballero-Mellado J,Moënne-Loccoz Y

更新日期：2009-05-01 00:00:00

abstract：:Currently used alum precipitated and oil adjuvant vaccines against HS caused by Pasteurella multocida B:2, have side effects and short-lived immunity, leading to regular catastrophic outbreaks in bovines in Asian subcontinent. The need for the development of an improved vaccine with longer immunity and the ability to ...

journal_title：Letters in applied microbiology

authors： Qureshi S,Saxena HM,Imam N,Kashoo Z,Sharief Banday M,Alam A,Malik MZ,Ishrat R,Bhat B

更新日期：2018-09-01 00:00:00

abstract：AIMS:An agar medium containing a range of related chlorophenoxyalkanoic acid herbicides, 2,4-dichlorophenoxyacetic acid (2,4-D), 2-methyl-4-chlorophenoxyacetic acid (MCPA), racemic mecoprop, (R)-mecoprop and racemic 2,4-DP (2-(2,4-dichlorophenoxy) propionic acid) was developed to assess the catabolic activity of a rang...

journal_title：Letters in applied microbiology

authors： Smejkal CW,Vallaeys T,Burton SK,Lappin-Scott HM

更新日期：2001-04-01 00:00:00

abstract：AIMS:To evaluate the genetic diversity within the species Paenibacillus polymyxa. METHODS:Southern hybridization was performed on 102 strains of P. polymyxa using DNA from the phage IPy1 as a probe. RESULTS:All 102 strains hybridized to phage IPy1 DNA. Data from different hybridization patterns obtained were used to ...

journal_title：Letters in applied microbiology

authors： Santos SC,Rodrigues Coelho MR,Seldin L

更新日期：2002-01-01 00:00:00

abstract：AIMS:To investigate the susceptibility of 221 Listeria spp. (86 Listeria monocytogenes, 41 Listeria innocua and 94 Listeria seeligeri-Listeria welshimeri-Listeria ivanovii) isolated from catfish fillets and processing environment to 15 antibiotics. METHODS AND RESULTS:Listeria isolates were analysed by disc-diffusion ...

journal_title：Letters in applied microbiology

authors： Chen BY,Pyla R,Kim TJ,Silva JL,Jung YS

更新日期：2010-06-01 00:00:00

abstract：AIMS:The present study describes the implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry. METHODS AND RESULTS:Real-time PCR with Salmonella invA-specific primers and a standard bacteriological method was applied to detect Salmonella in tetrathionate enrichment cult...

journal_title：Letters in applied microbiology

authors： Eyigor A,Carli KT,Unal CB

更新日期：2002-01-01 00:00:00