Influence of interactions between temperature, ferric ammonium citrate and glycine betaine on the growth of Listeria monocytogenes in a defined medium.

Abstract:

AIMS:To investigate interactions, if any, between temperature, ferric ammonium citrate and glycine betaine on the growth of Listeria monocytogenes in modified Pine's medium (Pine et al. 1989). METHODS AND RESULTS:Modified Pine's medium containing 0, 0.044, 0.088 or 0.176 g l(-1) ferric ammonium citrate, and 0 or 1 mM glycine betaine, was inoculated with each of two L. monocytogenes strains and incubated at 4, 25 or 37 degrees C. The optical density at 600 nm, and cell numbers, were determined at appropriate time intervals. At 4 degrees C, but not other temperatures, increasing ferric ammonium citrate resulted in improved growth in the absence, but not the presence, of glycine betaine. The presence of glycine betaine was inhibitory at 25 and 37 degrees C, but not at 4 degrees C. CONCLUSIONS:Interactions affecting the growth kinetics of L. monocytogenes were apparent between the parameters investigated. SIGNIFICANCE AND IMPACT OF THE STUDY:Limitations on the use of modified Pine's medium, and the significance of iron metabolism at lower temperatures, were revealed.

journal_name

Lett Appl Microbiol

authors

Dykes GA,Dworaczek M

doi

10.1046/j.1472-765x.2002.01237.x

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

538-42

issue

6

eissn

0266-8254

issn

1472-765X

pii

1237

journal_volume

35

pub_type

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