Performance of recombinant ESAT-6 antigen (ML0049) for detection of leprosy patients.

abstract：AIMS:To identify the abattoir source(s) of psychrophilic clostridia causing 'blown pack' spoilage of vacuum-packed chilled meats. METHODS AND RESULTS:Molecular procedures were used to detect the presence of specific 16S rRNA gene fragments of blown pack-causing clostridia in samples collected from a commercial abattoi...

journal_title：Letters in applied microbiology

authors： Boerema JA,Broda DM,Bell RG

更新日期：2003-01-01 00:00:00

abstract：AIMS:To identify and characterize amino acid transport in Leuconostoc mesenteroides. METHODS AND RESULTS:The transport of labelled amino acids was measured in whole cells of Leuc. mesenteroides CNRZ 1273. Systems were operative under physiological conditions of growth, energy dependent and differed from peptide transp...

journal_title：Letters in applied microbiology

authors： Gendrot F,Foucaud-Scheunemann C,Ferchichi M,Hemme D

更新日期：2002-01-01 00:00:00

abstract：AIMS:To examine changes in membrane fatty acid profile attributed to the physiological adaptation of Lactobacillus helveticus during vacuum drying. METHODS AND RESULTS:The viability and membrane integrity of the cells after vacuum drying were measured by plate counts and DNA fluorescence dyes. The physiological adapta...

journal_title：Letters in applied microbiology

authors： Santivarangkna C,Kulozik U,Kienberger H,Foerst P

更新日期：2009-10-01 00:00:00

abstract：AIM:To quantify the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 MG1655 cells at static temperatures. METHODS AND RESULTS:Stationary-phase E. coli cells were inactivated at 52, 54 and 58°C. The heat resistance is described as reduction in the inactivation rate, k(max) , and...

journal_title：Letters in applied microbiology

authors： Velliou EG,Van Derlinden E,Cappuyns AM,Aerts D,Nikolaidou E,Geeraerd AH,Devlieghere F,Van Impe JF

更新日期：2011-02-01 00:00:00

abstract：AIMS:To develop and evaluate a real-time PCR assay incorporating an internal amplification control (IAC) suitable for the screening of Shiga toxin (Stx)-producing Escherichia coli (STEC) in foods. METHODS AND RESULTS:A competitive IAC was constructed and included in an stx-specific real-time PCR assay. Coupled to 18-h...

journal_title：Letters in applied microbiology

authors： Auvray F,Lecureuil C,Dilasser F,Taché J,Derzelle S

更新日期：2009-05-01 00:00:00

abstract：AIMS:To investigate whether intranasal Lactobacillus administration protects host animals from influenza virus (IFV) infection by enhancing respiratory immune responses in a mouse model. METHODS AND RESULTS:After 3 days of intranasal exposure to Lactobacillus rhamnosus GG (LGG), BALB/c mice were infected with IFV A/PR...

journal_title：Letters in applied microbiology

authors： Harata G,He F,Hiruta N,Kawase M,Kubota A,Hiramatsu M,Yausi H

更新日期：2010-06-01 00:00:00

abstract：AIMS:To simplify the electrotransformation process of Clostridium acetobutylicum, which currently needs to be performed in an anaerobic chamber, thus laborious and time-consuming. METHODS AND RESULTS:The CAC2634 gene encoding PerR is a known peroxide regulon repressor in Cl. acetobutylicum. CAC2634 in a previously con...

journal_title：Letters in applied microbiology

authors： Dong H,Tao W,Zhu L,Zhang Y,Li Y

更新日期：2011-09-01 00:00:00

abstract：AIMS:The objective of this work was to purify the tyrosinase from Bacillusthuringiensis subsp. kurstaki (Bt) (CCTCC AB 90010) and study its enzymatic properties. METHODS AND RESULTS:A 'one-step' purification method was used in this work, which was an easy, high-yield purification method. Tyrosinase activity of this pu...

journal_title：Letters in applied microbiology

authors： Liu N,Zhang T,Wang YJ,Huang YP,Ou JH,Shen P

更新日期：2004-01-01 00:00:00

abstract：AIM:To determine if the outer membrane (OM) cytochromes OmcA and OmcB of the metal-reducing bacterium Shewanella oneidensis MR-1 have distinct or overlapping roles in the reduction of insoluble manganese(IV) oxide. METHODS AND RESULTS:The gene replacement mutant (OMCA1) which lacks OmcA was partially deficient in Mn(I...

journal_title：Letters in applied microbiology

authors： Myers JM,Myers CR

更新日期：2003-01-01 00:00:00

abstract：:While patients with cystic fibrosis (CF) have had dramatic improvement in their survival rates, this has been accompanied by the emergence of more virulent pathogens such as Pseudomonas aeruginosa and Burkholderia cepacia complex organisms. In addition, there has been emergence of organisms of increasing clinical sign...

journal_title：Letters in applied microbiology

authors： Jordan PW,Stanley T,Donnelly FM,Elborn JS,McClurg RB,Millar BC,Goldsmith CE,Moore JE

更新日期：2007-05-01 00:00:00

abstract：:Patterns of long-chain faecal fatty acids were studied by gas-liquid chromatography in 55 newborn infants in a neonatal intensive care unit. Decreased fractions of fatty acid C16:1 and increased fractions of C16:0 and C17:0 were associated with the occurrence of abdominal distension. Decreased fractions of C16:1 and C...

journal_title：Letters in applied microbiology

authors： Ahtonen P,Lehtonen OP,Kero P,Eerola E

更新日期：1997-02-01 00:00:00

abstract：AIMS:The risk of hepatitis C virus infection in hospital environments can be assessed not only by studying epidemiological data and work practices, but also by the detection of these viruses (or indicators thereof) in health-care settings, on instruments etc. METHODS:Since standardized techniques specific to this end ...

journal_title：Letters in applied microbiology

authors： Carducci A,Verani M,Casini B,Giuntini A,Mazzoni F,Rovini E,Passaglia A,Giusti L,Valenza A,Lombardi R

更新日期：2002-01-01 00:00:00

abstract：:In a crossover study, volunteers consumed a diet supplemented with either wheat or rye bread. Levels of faecal mucus were determined and the adhesion of five selected probiotics to immobilized intestinal mucus, was assessed. No significant difference in the concentration of faecal mucus or adhesion of the probiotics t...

journal_title：Letters in applied microbiology

authors： Ouwehand AC,Gråsten S,Niemi P,Mykkänen H,Salminen S

更新日期：2000-07-01 00:00:00

abstract：UNLABELLED:Syphilis is a multistage sexually transmitted disease that remains a serious public health concern worldwide. The coexistence of Treponema pallidum with other closely related members of spirochaeta, such as Leptospira spp. and Borrelia burgdorferi, has complicated the serodiagnosis due to cross-reactive anti...

journal_title：Letters in applied microbiology

authors： Xie Y,Xu M,Wang C,Xiao J,Xiao Y,Jiang C,You X,Zhao F,Zeng T,Liu S,Kuang X,Wu Y

更新日期：2016-04-01 00:00:00

abstract：AIMS:  To show that application of copper hydroxide citrus sprays mixed with field source water (possibly contaminated) will not support Escherichia coli on plant surfaces. Environmental stresses of transient phyllosphere bacteria and presence of copper will eradicate these bacteria before harvest. METHODS AND RESULTS...

journal_title：Letters in applied microbiology

authors： Narciso JA,Ference CM,Ritenour MA,Widmer WW

更新日期：2012-02-01 00:00:00

abstract：:Entamoeba histolytica and Entamoeba dispar are two microscopically indistinguishable amoebae living in the human colon. The former is a pathogen, whereas the latter is a nonpathogenic commensal. Using a model system of in vitro cocultures and PCR detection of the Entamoeba species, we found that the nonpathogenic spec...

journal_title：Letters in applied microbiology

authors： Pysova I,Tumova P,Tolarova V,Nohynkova E

更新日期：2009-04-01 00:00:00

abstract：:This study evaluated the performance of CPS ID2 (bioMérieux) compared to that of conventionally used selective agar for the identification of bacteria responsible for urinary tract infections. This medium detects bacterial enzymes using chromogenic substrates. Two hundred and nineteen samples of urine were tested in o...

journal_title：Letters in applied microbiology

authors： Willinger B,Manafi M

更新日期：1995-05-01 00:00:00

abstract：:Shigella spp. are water-borne pathogens responsible for mild to severe cases bacilli dysentery all around the world known as Shigellosis. The progressively increasing of antibiotic resistance among Shigella calls for developing and establishing novel alternative therapeutic methods. The present study aimed to evaluate...

journal_title：Letters in applied microbiology

authors： Shahin K,Zhang L,Bao H,Hedayatkhah A,Soleimani-Delfan A,Komijani M,He T,Barazandeh M,Mansoorianfar M,Bouzari M,Wang R

更新日期：2020-10-18 00:00:00

abstract：AIMS:To evaluate the effect of protective agents upon survival of Lactobacillus delbrueckii ssp. bulgaricus during freeze-drying and storage, and selective amino acids on cell membrane fluidity. METHODS AND RESULTS:The protective effect of amino-acids and sugars at different concentrations was studied by determining t...

journal_title：Letters in applied microbiology

authors： Martos GI,Minahk CJ,de Valdez GF,Morero R

更新日期：2007-09-01 00:00:00

abstract：:Autoaggregation was frequently encountered among intestinal lactobacilli isolated from weaned pigs. The aggregation mechanism was shown to be mediated by the production of a proteinaceous aggregation-promoting factor in two strains of Lactobacillus reuteri. A 32 kDa aggregation-promoting protein was detected in these ...

journal_title：Letters in applied microbiology

authors： Kmet V,Callegari ML,Bottazzi V,Morelli L

更新日期：1995-12-01 00:00:00

abstract：AIMS: To develop a new instrument of great versatility for recovering micro-organisms from all types of food samples and to compare the effects with existing sample preparation methods. METHODS AND RESULTS: To detach micro-organisms from large-size unbroken food samples such as apples, carrots, potatoes and tomatoes w...

journal_title：Letters in applied microbiology

authors： Kim YH,Lee SY,Sagong HG,Heu S,Ryu S,Kang DH

更新日期：2012-09-01 00:00:00

abstract：:A simple artificial urine medium (AUM) has been developed which provides conditions similar to that found in human urine. AUM solidified with agar enabled the recovery of a wide range of urease-positive and -negative urinary pathogens. Liquid AUM supported growth at concentrations of up to 10(8) cfu ml-1, as found in ...

journal_title：Letters in applied microbiology

authors： Brooks T,Keevil CW

更新日期：1997-03-01 00:00:00

abstract：:Candida species are increasingly important fungal pathogens. The reaction of rat alveolar macrophages (AM) to Candida albicans was compared with that to C. glabrata and C. krusei. Phagocytosis of C. glabrata was similar to that of C. albicans, but significantly slower for C. krusei due to reduced attachment. After ops...

journal_title：Letters in applied microbiology

authors： Nessa K,Johansson A,Jarstrand C,Camner P

更新日期：1997-09-01 00:00:00

abstract：AIMS:The purpose of this study was to develop a loop-mediated isothermal amplification (LAMP) method for the rapid, sensitive and simple detection of Vibrio alginolyticus in mariculture fish. METHODS AND RESULTS:LAMP primers were designed by targeting the gyrB gene. With Bst DNA polymerase, the target DNA can be clear...

journal_title：Letters in applied microbiology

authors： Cai SH,Lu YS,Wu ZH,Jian JC,Wang B,Huang YC

更新日期：2010-05-01 00:00:00

abstract：AIMS:To determine whether nonspore-forming biothreat agents can survive and persist in potable water that does not contain a disinfectant. METHODS AND RESULTS: Autoclaved, de-chlorinated Atlanta municipal water was inoculated with eight isolates of bacterial biothreat agents (10⁶ CFU ml⁻¹). The inoculated water sample...

journal_title：Letters in applied microbiology

authors： Gilbert SE,Rose LJ

更新日期：2012-09-01 00:00:00

abstract：AIMS:The antigen 85 complex (Ag85) from Mycobacterium tuberculosis consists of three abundantly secreted proteins (FbpA, FbpB and FbpC2) which play a key role in the pathogenesis of tuberculosis and also exhibit cell wall mycolyltransferase activity. A related protein with similarity to the Ag85 complex was recently an...

journal_title：Letters in applied microbiology

authors： Kremer L,Maughan WN,Wilson RA,Dover LG,Besra GS

更新日期：2002-01-01 00:00:00

abstract：AIMS:To investigate the incidence of Campylobacter and Salmonella contamination associated with supermarket and butchers' shop chicken and related packaging. METHOD AND RESULTS:Three hundred raw samples (whole chicken, chicken breast with skin or chicken pieces) were purchased on a monthly basis for seven months. Pack...

journal_title：Letters in applied microbiology

authors： Harrison WA,Griffith CJ,Tennant D,Peters AC

更新日期：2001-12-01 00:00:00

abstract：AIMS:To investigate a lactococcal plasmid responsible for a reduction in growth rate of its host cell. METHODS AND RESULTS:Lactococcus lactis subsp. lactis biovar. diacetylactis DRC1 carries a high number of plasmids. The DRC1 wild-type strain was found to grow more slowly than a plasmid-free derivative of DRC1. The p...

journal_title：Letters in applied microbiology

authors： Kobayashi M,Nomura M,Fujita Y,Okamoto T,Ohmomo S

更新日期：2002-01-01 00:00:00

abstract：:The ability of Stenotrophomonas maltophilia strain VUN 10,003 to degrade and detoxify high molecular weight polycyclic aromatic hydrocarbons (PAHs) was evaluated in a basal liquid medium. Using high cell density inocula of strain VUN 10,003, the concentration of pyrene, fluoranthene, benz[a]anthracene, benzo[a]pyrene,...

journal_title：Letters in applied microbiology

authors： Juhasz AL,Stanley GA,Britz ML

更新日期：2000-05-01 00:00:00

abstract：AIMS:To investigate the effect of molasses concentration, initial pH of molasses medium, and inoculum's size to maximize ethanol and minimize methanol, fusel alcohols, acetic acid and aldehydes in the fermentation mash in industrial fermentors. METHODS AND RESULTS:Initial studies to optimize temperature, nitrogen sour...

journal_title：Letters in applied microbiology

authors： Arshad M,Khan ZM,Khalil-ur-Rehman,Shah FA,Rajoka MI

更新日期：2008-11-01 00:00:00