Intranasal administration of Lactobacillus rhamnosus GG protects mice from H1N1 influenza virus infection by regulating respiratory immune responses.

Abstract:

AIMS:To investigate whether intranasal Lactobacillus administration protects host animals from influenza virus (IFV) infection by enhancing respiratory immune responses in a mouse model. METHODS AND RESULTS:After 3 days of intranasal exposure to Lactobacillus rhamnosus GG (LGG), BALB/c mice were infected with IFV A/PR/8/34 (H1N1). Mice treated with LGG showed a lower frequency of accumulated symptoms and a higher survival rate than control mice (P < 0.05). The YAC-1 cell-killing activity of lung cells isolated from mice treated with LGG was significantly greater than those isolated from control mice (P < 0.01). Intranasal administration of LGG significantly increased mRNA expression of interleukin (IL)-1 beta, tumour necrosis factor (TNF) and monocyte chemotactic protein (MCP)-1 (P < 0.01). CONCLUSIONS:These results suggest that intranasal administration of LGG protects the host animal from IFV infection by enhancing respiratory cell-mediated immune responses following up-regulation of lung natural killer (NK) cell activation. SIGNIFICANCE AND IMPACT OF STUDY:We have demonstrated that probiotics might protect host animals from viral infection by stimulating immune responses in the respiratory tract.

journal_name

Lett Appl Microbiol

authors

Harata G,He F,Hiruta N,Kawase M,Kubota A,Hiramatsu M,Yausi H

doi

10.1111/j.1472-765X.2010.02844.x

subject

Has Abstract

pub_date

2010-06-01 00:00:00

pages

597-602

issue

6

eissn

0266-8254

issn

1472-765X

pii

LAM2844

journal_volume

50

pub_type

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