Barotolerance is dependent on both trehalose and heat shock protein 104 but is essentially different from thermotolerance in Saccharomyces cerevisiae.

abstract：:A sandwich enzyme-linked immunosorbent assay to detect staphylococcal enterotoxin B was developed using rat monoclonal antibodies as capture antibodies and as a biotinylated conjugate. This test was sensitive, less than 1 ng/ml of enterotoxin B was detected and interference by protein A was prevented by the use of rat...

journal_title：Letters in applied microbiology

authors： Rogemond V,Da Costa Castro JM,Delaunay T,Cazaux C,Guinet RM

更新日期：1991-09-01 00:00:00

abstract：AIMS:The objective of the present study was to determine the optimal culture conditions for exo-biopolymer production by Cordyceps militaris in shake flask culture. METHODS AND RESULTS:The optimal temperature and initial pH for both mycelial growth and exo-biopolymer production by Cordyceps militaris in shake flask cu...

journal_title：Letters in applied microbiology

authors： Park JP,Kim SW,Hwang HJ,Yun JW

更新日期：2001-07-01 00:00:00

abstract：:The antimicrobial activities of bovine lactoferrin (bLF), its pepsin hydrolysate (bLFH) and the active peptide lactoferricin B (LFcinB) against four clinical isolates of enterohaemorrhagic Escherichia coli O157:H7 were studied. The MICs against these isolates were 3 mg ml-1 for bLF, 0.1-0.2 mg ml-1 for bLFH and 8-10 m...

journal_title：Letters in applied microbiology

authors： Shin K,Yamauchi K,Teraguchi S,Hayasawa H,Tomita M,Otsuka Y,Yamazaki S

更新日期：1998-06-01 00:00:00

abstract：AIMS:To provide evidence that DNA-PCR diagnostics of oral pathogens based on standard sample collection by paper point insertion from the depth of the periodontal pocket can be replaced by a novel non-invasive collection method based on swab technique from the gingiva. METHODS AND RESULTS:In this study we compared the...

journal_title：Letters in applied microbiology

authors： Smola SF,Rettenberger G,Simmet T,Burysek L

更新日期：2003-01-01 00:00:00

abstract：:Increasingly, molecular methods have become important in identification and confirmation of bacteria at the species level. Rapid molecular methods provide sensitivity and specificity while reducing cost and resources. The primary goal of this study was to develop a real-time PCR assay for identification of Escherichia...

journal_title：Letters in applied microbiology

authors： Tillman GE,Simmons M,Wasilenko JL,Narang N,Cray WC Jr,Bodeis-Jones S,Martin G,Gaines S,Seal BS

更新日期：2015-02-01 00:00:00

abstract：AIMS:To determine the production of reuterin by lactobacilli isolates from pig faeces and to evaluate their potential as probiotic bacteria. METHODS AND RESULTS:Twenty-eight of 165 lactobacilli isolates produced reuterin in the presence of glycerol. Six isolates yielding high levels of reuterin with respect to type st...

journal_title：Letters in applied microbiology

authors： Rodríguez E,Arqués JL,Rodríguez R,Nuñez M,Medina M

更新日期：2003-01-01 00:00:00

abstract：:Nicotinic acid (NA) is ubiquitous in nature and its microbial degradation mechanisms are diverse. In this study, Pusillimonas sp. strain T2 was found to be capable of utilizing NA as sole carbon and nitrogen sources. This strain could completely degrade 300 mg l-1 NA within 3·5 h at 30°C and pH 7·0 and one of the degr...

journal_title：Letters in applied microbiology

authors： Yuan M,Zhang Y,Zhao L,Ma Y,He Q,He J,Qiu J

更新日期：2018-04-01 00:00:00

abstract：AIMS:To screen for the existence and determine the structure of Tn916-like element in Streptococcus parauberis serotype II strains isolated from cultured Japanese flounder in western Japan. METHODS AND RESULTS:In this study, the structure of Tn916-like element and the flanking regions were characterized by polymerase ...

journal_title：Letters in applied microbiology

authors： Meng F,Kanai K,Yoshikoshi K

更新日期：2009-06-01 00:00:00

abstract：:The yield of purified recombinant alpha-sarcin increases approximately three- to fourfold when this toxin is co-expressed in Escherichia coli with thioredoxin. This increased production is attributed to the existence, in the presence of thioredoxin, of a reducing environment which allows rearrangement of incorrect dis...

journal_title：Letters in applied microbiology

authors： García-Ortega L,Lacadena J,Lacadena V,Masip M,De Antonio C,Martínez-Ruiz A,Martínez Del Pozo A

更新日期：2000-04-01 00:00:00

abstract：:Limited data are available on the prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in the UK. We tested 124 raw meat samples for MRSA including pork (n = 63), chicken (n = 50) and turkey (n = 11) collected from retail outlets in North West England between March and July 2015. MR...

journal_title：Letters in applied microbiology

authors： Fox A,Pichon B,Wilkinson H,Doumith M,Hill RL,McLauchlin J,Kearns AM

更新日期：2017-03-01 00:00:00

abstract：AIMS:To measure the microbial removal capacity of a small-scale hydroponics wastewater treatment plant. METHODS AND RESULTS:Paired samples were taken from untreated, partly-treated and treated wastewater and analysed for faecal microbial indicators, i.e. coliforms, Escherichia coli, enterococci, Clostridium perfringen...

journal_title：Letters in applied microbiology

authors： Ottoson J,Norström A,Dalhammar G

更新日期：2005-01-01 00:00:00

abstract：AIMS:Alpha-galactosidase is applied in food and feed industries for hydrolysing raffinose series oligosaccharides (RO) that are the factors primarily responsible for flatulence upon ingestion of soybean-derived products. The objective of the current work was to develop an optimal culture medium for the production of al...

journal_title：Letters in applied microbiology

authors： Liu CQ,Chen QH,Tang B,Ruan H,He GQ

更新日期：2007-08-01 00:00:00

abstract：:The emergence of an increasing number of antibiotic resistant human clinical bacteria has been a great cause of concern for the last decades. As an example, Staphylococcus aureus isolates in the hospital environment are becoming more and more resistant to antibiotics including vancomycin which is considered as a last ...

journal_title：Letters in applied microbiology

authors： Rihakova J,Belguesmia Y,Petit VW,Pilet MF,Prévost H,Dousset X,Drider D

更新日期：2009-01-01 00:00:00

abstract：AIMS:In the present study, two different optimization techniques were used to determine the suitable operating parameters for exo-biopolymer production in submerged mycelial cultures of two entomopathogenic fungi Paecilomyces japonica and Paecilomyces tenuipes. METHODS AND RESULTS:First, the rotating simplex method, a...

journal_title：Letters in applied microbiology

authors： Xu CP,Sinha J,Bae JT,Kim SW,Yun JW

更新日期：2006-05-01 00:00:00

abstract：UNLABELLED:Most of the current research into the quantification of soil-borne pathogenic oomycetes lacks determination of DNA extraction efficiency, probably leading to an incorrect estimation of DNA quantity. In this study, we developed a convenient method by using a 100 bp artificially synthesized DNA sequence derive...

journal_title：Letters in applied microbiology

authors： Li M,Ishiguro Y,Kageyama K,Zhu Z

更新日期：2015-08-01 00:00:00

abstract：AIMS:The use of randomly amplified polymorphic DNA (RAPD)-PCR fingerprinting and plasmid profiles to determine at the strain level, the similarity of Lactococcus lactis isolates obtained during sampling of traditional cheeses and to verify its correspondence to the selected phenotypic characteristics. METHODS AND RESU...

journal_title：Letters in applied microbiology

authors： de la Plaza M,Rodríguez A,Fernández de Palencia P,Martínez-Cuesta MC,Peláez C,Requena T

更新日期：2006-12-01 00:00:00

abstract：AIMS:This paper attempts to develop a kinetic model to describe the growth of aerobic granules developed under different operation conditions. METHODS AND RESULTS:A series of experiments were conducted by using four-column sequencing batch reactors to study the formation of aerobic granules under different conditions,...

journal_title：Letters in applied microbiology

authors： Yang SF,Liu QS,Tay JH,Liu Y

更新日期：2004-01-01 00:00:00

abstract：:A set of Escherichia coli freshwater isolates was chosen to compare the effectiveness of denaturing gradient gel electrophoresis (DGGE) vs temporal temperature gradient gel electrophoresis (TTGE) for separating homologous amplicons from the respective uidA region differing in one to seven single base substitutions. Bo...

journal_title：Letters in applied microbiology

authors： Farnleitner AH,Kreuzinger N,Kavka GG,Grillenberger S,Rath J,Mach RL

更新日期：2000-06-01 00:00:00

abstract：UNLABELLED:Escherichia coli is part of the normal microflora of the intestines of mammals. However, among the enteric pathogens, it is one of the leading causes of intestinal diseases, especially Shiga toxigenic E. coli, which can cause diarrhoea, haemorrhagic colitis and complications like haemolytic uraemic syndrome ...

journal_title：Letters in applied microbiology

authors： Ribeiro LF,Barbosa MM,de Rezende Pinto F,Guariz CS,Maluta RP,Rossi JR,Rossi GA,Lemos MV,do Amaral LA

更新日期：2016-03-01 00:00:00

abstract：AIM:The aim of this work was to evaluate the effect of Planococcus ficus infection in red wine grapes on Aspergillus section Nigri and ochratoxin A (OTA) contamination. METHODS AND RESULTS:During 2006/2007 and 2008/2009 vintages, Merlot, Malbec and Cabernet Sauvignon varieties divided into two categories of grape samp...

journal_title：Letters in applied microbiology

authors： Chiotta ML,Ponsone ML,Torres AM,Combina M,Chulze SN

更新日期：2010-08-01 00:00:00

abstract：AIMS:Saccharomyces cerevisiae was used for studying the physiological effects of hydrostatic pressure. METHODS AND RESULTS:The effects of hydrostatic pressure on the ultrastructure of wild-type and trehalose-6-phosphate synthase (tps1) mutant cells were investigated by transmission electron microscopy. Pressure induce...

journal_title：Letters in applied microbiology

authors： Fernandes PM,Farina M,Kurtenbach E

更新日期：2001-01-01 00:00:00

abstract：:Colicin V is a small, proteinaceous bacterial toxin, produced by many strains of Escherichia coli and other members of the Enterobacteriaceae, that fits the definition of class II bacteriocins of Gram-positive bacteria. Export of colicin V is dependent on specific ABC (ATP-binding cassette) secretion proteins which re...

journal_title：Letters in applied microbiology

authors： McCormick JK,Klaenhammer TR,Stiles ME

更新日期：1999-07-01 00:00:00

abstract：:Sodium pyrithione and zinc pyrithione (NaPT and ZnPT, respectively) are widely used as cosmetic preservatives and metal chelating agents. They are commonly assayed using thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). However, a simple quantitative colorimetric assay has not been pre...

journal_title：Letters in applied microbiology

authors： Dinning AJ,al-Adham IS,Austin P,Collier PJ

更新日期：1998-07-01 00:00:00

abstract：UNLABELLED:Staphylococcus aureus LZ-01 was isolated from the Yellow River upstream from Lanzhou which can resist and reduce chromium (VI) to chromium (III). In this study, strain LZ-01's uranium (VI) resistance and adsorption abilities were investigated. Our results showed that it can resist 2 mmol l(-1) U(VI) and adso...

journal_title：Letters in applied microbiology

authors： Zou L,Chen Z,Zhang X,Liu P,Li X

更新日期：2014-11-01 00:00:00

abstract：:The small cryptic plasmid pMB1 (1.9 kb), previously isolated from Bifidobacterium longum, has been characterized by physical mapping. Two cloning vectors, pMR3 and pDG7, carrying chloramphenicol and ampicillin resistances derived from pJH101, have been electroporated in Escherichia coli. ...

journal_title：Letters in applied microbiology

authors： Matteuzzi D,Brigidi P,Rossi M,Di D

更新日期：1990-10-01 00:00:00

abstract：AIMS:To establish species-specific PCR and DNA probe methods for Streptococcus salivarius and to clarify the distribution of dextranase in oral isolates of Strep. salivarius. METHODS AND RESULTS:A pair of PCR primers and a DNA probe were designed based on the nucleotide sequence of the dextranase gene of Strep. saliva...

journal_title：Letters in applied microbiology

authors： Igarashi T,Yano Y,Yamamoto A,Sasa R,Goto N

更新日期：2001-06-01 00:00:00

abstract：AIMS:Aims of investigation: (i) develop a quantitative RT-PCR for noroviruses and (ii) evaluate it on environmental samples. METHODS AND RESULTS:Noroviruses in environmental water samples were concentrated by adsorption/elution/flocculation. Sewage was processed by clarification and protein flocculation. Norovirus-spe...

journal_title：Letters in applied microbiology

authors： Laverick MA,Wyn-Jones AP,Carter MJ

更新日期：2004-01-01 00:00:00

abstract：AIMS:Bacillus halodurans C-125 is a Gram-positive bacterium that was the first alkaliphilic species to have its genome completely sequenced. Despite its many years as a model for alkaliphily and source of industrially important enzymes, genetic manipulation of B. halodurans C-125 remains difficult, and therefore, we so...

journal_title：Letters in applied microbiology

authors： Wallace JG,Breaker RR

更新日期：2011-04-01 00:00:00

abstract：AIMS:The present study describes the implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry. METHODS AND RESULTS:Real-time PCR with Salmonella invA-specific primers and a standard bacteriological method was applied to detect Salmonella in tetrathionate enrichment cult...

journal_title：Letters in applied microbiology

authors： Eyigor A,Carli KT,Unal CB

更新日期：2002-01-01 00:00:00

abstract：UNLABELLED:This study reports the development of a loop-mediated isothermal amplification (LAMP) reaction for the detection of Pythium myriotylum. The primer set targeting the ITS sequence of P. myriotylum worked most efficiently at 60°C and allowed the detection of P. myriotylum DNA within 30 min by fluorescence monit...

journal_title：Letters in applied microbiology

authors： Fukuta S,Takahashi R,Kuroyanagi S,Ishiguro Y,Miyake N,Nagai H,Suzuki H,Tsuji T,Hashizume F,Watanabe H,Kageyama K

更新日期：2014-07-01 00:00:00