Discrepancies between the phenotypic and genotypic characterization of Lactococcus lactis cheese isolates.

Abstract:

AIMS:The use of randomly amplified polymorphic DNA (RAPD)-PCR fingerprinting and plasmid profiles to determine at the strain level, the similarity of Lactococcus lactis isolates obtained during sampling of traditional cheeses and to verify its correspondence to the selected phenotypic characteristics. METHODS AND RESULTS:A total of 45 L. lactis isolates were genotypically analysed by RAPD-PCR fingerprinting and plasmid patterns. Phenotypic traits used to compare strains were proteolytic, acidifying, aminotransferase (aromatic and branched chain aminotransferase) and alpha-ketoisovalerate decarboxylase (Kivd) activities. The results show that 23 isolates could be grouped in clusters that exhibited 100% identity in both their RAPD and plasmid patterns, indicating the probable isolation of dominant strains during the cheese sampling process. However, there were phenotypic differences between isolates within the same cluster that included the loss of relevant technological properties such as proteinase activity and acidifying capacity or high variation in their amino acid converting enzyme activities. Likewise, the analysis of a specific attribute, Kivd activity, indicated that 7 of 15 isolates showed no detectable activity despite the presence of the encoding (kivd) gene. CONCLUSION:Phenotypic differences found between genotypically similar strains of L. lactis strains could be linked to differences in enzymatic expression. SIGNIFICANCE AND IMPACT OF THE STUDY:Phenotypic analysis of L. lactis isolates should be considered when selecting strains with new cheese flavour forming capabilities.

journal_name

Lett Appl Microbiol

authors

de la Plaza M,Rodríguez A,Fernández de Palencia P,Martínez-Cuesta MC,Peláez C,Requena T

doi

10.1111/j.1472-765X.2006.02012.x

subject

Has Abstract

pub_date

2006-12-01 00:00:00

pages

637-44

issue

6

eissn

0266-8254

issn

1472-765X

pii

LAM2012

journal_volume

43

pub_type

杂志文章
  • Electronic paramagnetic resonance investigation of the activity of Origanum vulgare L. essential oil on the Listeria monocytogenes membrane.

    abstract:AIMS:To evaluate the effect of oregano essential oil on Listeria monocytogenes cytoplasmic membrane. METHODS AND RESULTS:Nitroxide free-radical Electron Paramagnetic Resonance was applied on L. monocytogenes after 30 min exposure to oregano essential oil concentrations ranging from 0 to 1.25%. The impact of essential ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2010.02877.x

    authors: Serio A,Chiarini M,Tettamanti E,Paparella A

    更新日期:2010-08-01 00:00:00

  • New media for detection and counting of Clostridia in foods.

    abstract::The growth of pure cultures of Clostridium perfringens (ATCC 12922) and Cl. sporogenes (PA 3679) in five non-selective media, fluid thioglycollate medium (FTM), rapid perfringens medium (RPM), Columbia broth Malthus (CBM), reinforced clostridial medium (RCM) and lactose sulphite (LS), was monitored using conductance m...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1997.00330.x

    authors: Dromigny E,Bourrion F,Rugraf Y,Bolton FJ,Leden N

    更新日期:1997-01-01 00:00:00

  • Bidirectional transformation of aromatic aldehydes by Desulfovibrio desulfuricans under nitrate-dissimilating conditions.

    abstract::Desulfovibrio desulfuricans ATCC 27774 was screened for reactivity against aromatic compounds during lactate-dependent, nitrate-dissimilating growth. Only aromatic aldehydes (benzaldehyde, 2-hydroxybenzaldehyde, 3-hydroxybenzaldehyde, 4-hydroxybenzaldehyde, vanillin, iso-vanillin and o-vanillin) were reactive and, wit...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1996.tb01122.x

    authors: Parekh M,Drake HL,Daniel SL

    更新日期:1996-02-01 00:00:00

  • Isolation and characterization of lactic acid bacteria from dochi (fermented black beans), a traditional fermented food in Taiwan.

    abstract:AIMS:To isolate, characterize and identify lactic acid bacteria (LAB) in dochi (fermented black beans), a traditional fermented food in Taiwan. METHODS AND RESULTS:A total of 30 samples were collected from three different dochi producers and analysed after different periods of storage. Fifty-two cultures of LAB were i...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.01922.x

    authors: Chen YS,Yanagida F,Hsu JS

    更新日期:2006-08-01 00:00:00

  • Evaluation of the BBL Crystal Enteric/Nonfermenter kit for the identification of water-derived environmental Enterobacteriaceae.

    abstract::The Crystal Enteric/Nonfermenter (E/NF) identification kit (Becton Dickinson Microbiology Systems, USA) was evaluated using water-derived bacterial isolates and results compared to those obtained by the API 20E system (BioMérieux, UK). Both the E/NF and 20E systems correctly identified 93% of the Enterobacteriaceae re...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1995.tb01031.x

    authors: Micklewright IJ,Sartory DP

    更新日期:1995-09-01 00:00:00

  • The effect of colony age on PCR fingerprinting.

    abstract::The pattern of DNA fragments produced by the polymerase chain reaction using arbitrary primers is strongly influenced by the age of the colonies sampled. We show that the number, clarity and reproducibility of the bands produced is optimal when very young colonies are sampled. ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1993.tb01467.x

    authors: Coutinho HL,Handley BA,Kay HE,Stevenson L,Beringer JE

    更新日期:1993-12-01 00:00:00

  • Selection of Salmonella typhimurium as an indicator for pathogen regrowth potential in composted biosolids.

    abstract::In order to select a suitable indicator for monitoring the pathogen regrowth potential of composted biosolids, the growth kinetics of selected bacteria were investigated. Growth parameters of six serovars of Salmonella and three strains of Escherichia coli in sterilized compost were compared. Seeded Salmonella and E. ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00626.x

    authors: Sidhu J,Gibbs RA,Ho GE,Unkovich I

    更新日期:1999-11-01 00:00:00

  • Optimization of glycosidases production by Pseudoalteromonas issachenkonii KMM 3549(T).

    abstract:AIMS:The present work aimed to design an optimized medium to yield a higher production of glycosides by Pseudoalteromonas issachenkonii KMM 3549(T). METHODS AND RESULTS:Higher levels of fucoidan hydrolase, alginase, laminaranase and b-N-acetylglucosaminidase production were obtained with peptone concentrations ranging...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01189.x

    authors: Alexeeva YV,Ivanova EP,Bakunina IY,Zvaygintseva TN,Mikhailov VV

    更新日期:2002-01-01 00:00:00

  • Evidence on inhibition of Listeria monocytogenes by divercin V41 action.

    abstract:AIMS:The aim of this study was to investigate the role of divercin V41 in inhibition and prevention of Listeria monocytogenes. METHODS AND RESULTS:Carnobacterium divergens V41 deficient in bacteriocin production was isolated and characterized by enzyme-liked immunosorbent assay, multiplex polymerase chain reaction and...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01310.x

    authors: Richard C,Brillet A,Pilet MF,Prévost H,Drider D

    更新日期:2003-01-01 00:00:00

  • Plasmid-mediated dimethoate degradation in Pseudomonas aeruginosa MCMB-427.

    abstract:AIMS:To investigate the genetics of dimethoate degradation in Pseudomonas aeruginosa MCMB-427. METHODS AND RESULTS:Pseudomonas aeruginosa MCMB-427 demonstrated the ability to degrade dimethoate, a synthetic organophosphate insecticide. Total DNA preparation of MCMB-427 revealed the presence of a 6.6 kbp plasmid (desig...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2001.00995.x

    authors: Deshpande NM,Dhakephalkar PK,Kanekar PP

    更新日期:2001-10-01 00:00:00

  • Lecanicillium aphanocladii: snake venom phospholipases A2 and proteases as tools to prospect enzymatic inhibitors.

    abstract::Natural enzyme inhibitors have been widely described in literature because of its pharmacological and cosmetic applications. Fungi found in caves represent a promising source of bioactive substances that are still little explored scientifically. Thus, the present work evaluated the presence of enzymatic modulators in ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13171

    authors: Cardoso MGB,Trento MVC,Reis CH,Marcussi S,Cardoso PG

    更新日期:2019-08-01 00:00:00

  • Differential activity profiles of translation inhibitors in whole-cell and cell-free approaches.

    abstract:AIMS:Evaluation of the activity profiles of standard prokaryotic translation inhibitors with different physicochemical properties under whole-cell and cell-free conditions. METHODS AND RESULTS:The minimal inhibitory concentration values (cell-free/whole-cell microg ml(-1)) for three aminoglycosides (neomycin, 0.01/6.9...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02281.x

    authors: Weidlich M,Klammt C,Bernhard F,Karas M,Stein T

    更新日期:2008-02-01 00:00:00

  • The gene coding for nigrescin produced by Prevotella nigrescens ATCC 25261.

    abstract:AIM:To identify the gene that encodes nigrescin, a bacteriocin produced by Prevotella nigrescens ATCC 25261. METHODS AND RESULTS:Each open reading frame (ORF) of the nig gene cluster (nigA, nigB, nigC and nigD) was transferred into an expression vector. The recombinant proteins encoded by nigA, nigB, nigC and nigD wer...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2009.02657.x

    authors: Teanpaisan R,Narawatthana S,Utarabhand P

    更新日期:2009-09-01 00:00:00

  • Antigenicity and N-terminal amino acid sequence of a 35 kDa porin-like protein of Listonella (Vibrio) anguillarum: comparison among different serotypes and other bacterial species.

    abstract::Listonella (Vibrio) anguillarum, an important fish pathogen, is divided into 10 serotypes according to O-antigens present on the outer membrane. However, the biochemical and immunological properties of porin proteins have not been reported. In this study, the antigenicity and N-terminal amino acid sequence of the 35 k...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1996.tb00195.x

    authors: Suzuki S,Kuroe K,Yasue K,Kusuda R

    更新日期:1996-11-01 00:00:00

  • Microbial diversity and biochemical characteristics of Borassus akeassii wine.

    abstract:UNLABELLED:Palm wine produced traditionally and consumed by many people in the South-West of Burkina Faso is subject to alteration. In this study, we carried out a follow-up of two palm wines' fermentation during the 10 days in which palm wines are classically produced and consumed. We monitored biochemical characteris...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12619

    authors: Tapsoba F,Savadogo A,Legras JL,Zongo C,Traore AS

    更新日期:2016-10-01 00:00:00

  • Isolation of a strain of Bacillus thuringiensis ssp. kurstaki HD-1 encoding delta-endotoxin Cry1E.

    abstract::A strain of Bacillus thuringiensis, STB-1, toxic against Spodoptera exigua, was isolated. Bacillus thuringiensis STB-1 produced bipyramidal inclusions and reacted with the H antiserum of B. thuringiensis ssp. kurstaki. The plasmid and protein profiles of B. thuringiensis STB-1 were compared with those of its reference...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1998.00355.x

    authors: Chang JH,Roh JY,Je YH,Park HW,Jin BR,Woo SD,Kang SK

    更新日期:1998-05-01 00:00:00

  • Detection and quantification of Aspergillus ochraceus in green coffee by PCR.

    abstract:AIMS:The aim of this study was to detect and quantify DNA of the ochratoxinogenic fungus Aspergillus ochraceus in green coffee and to compare the results with the ochratoxin A content of naturally contaminated samples. METHODS AND RESULTS:A DNA extraction protocol based on a combination of ultrasonification and a comm...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2004.01524.x

    authors: Schmidt H,Bannier M,Vogel RF,Niessen L

    更新日期:2004-01-01 00:00:00

  • Hydroxyaldimines as potent in vitro anticryptococcal agents.

    abstract:UNLABELLED:Cryptococcosis, a fungal infection that affects both immunocompromised and immunocompetent individuals, contributes to increasing indices of mortality and morbidity. The development of resistance by Cryptococcus spp., the limited number of commercial antifungal drugs and the various side effects of these dru...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12086

    authors: Magalhães TF,da Silva CM,de Fátima Â,da Silva DL,Modolo LV,Martins CV,Alves RB,Ruiz AL,Longato GB,de Carvalho JE,de Resende-Stoianoff MA

    更新日期:2013-08-01 00:00:00

  • Antibacterial activity of bovine lactoferrin and its peptides against enterohaemorrhagic Escherichia coli O157:H7.

    abstract::The antimicrobial activities of bovine lactoferrin (bLF), its pepsin hydrolysate (bLFH) and the active peptide lactoferricin B (LFcinB) against four clinical isolates of enterohaemorrhagic Escherichia coli O157:H7 were studied. The MICs against these isolates were 3 mg ml-1 for bLF, 0.1-0.2 mg ml-1 for bLFH and 8-10 m...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1998.00358.x

    authors: Shin K,Yamauchi K,Teraguchi S,Hayasawa H,Tomita M,Otsuka Y,Yamazaki S

    更新日期:1998-06-01 00:00:00

  • Antibiotic susceptibility of potentially probiotic Bifidobacterium isolates from the human gastrointestinal tract.

    abstract::Sixteen Bifidobacterium isolates from the human gastrointestinal tract were assayed for susceptibility to 44 antibiotics by soft agar overlay disc diffusion on TPY agar. Five isolates (3/7 B. bifidum and 2/3 B. breve) exhibited atypical antibiotic susceptibility profiles. Poor growth in the agar overlay accounted for ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1998.00342.x

    authors: Charteris WP,Kelly PM,Morelli L,Collins JK

    更新日期:1998-05-01 00:00:00

  • Testing the performance of microbiological safety cabinets used in microbiology laboratories in South Korea.

    abstract:AIMS:To test a performance of the microbiological safety cabinets (MSCs) according to the type of MSCs in microbial laboratories. METHODS AND RESULTS:Tests were carried out to assess the performance of 31 MSCs in 14 different facilities, including six different biological test laboratories in six hospitals and eight d...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2011.03101.x

    authors: Hwang SH,Yi TW,Cho KH,Lee IM,Yoon CS

    更新日期:2011-09-01 00:00:00

  • Application of ARISA to assess the influence of salt content and cation type on microbiological diversity of Cheddar cheese.

    abstract:UNLABELLED:The structure and dynamics of microbial populations play a significant role during cheese manufacture and ripening. Therefore, fast and accurate methods for identification and characterization of the microbial populations are of fundamental importance to the cheese industry. In this study, we investigate the...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12263

    authors: Porcellato D,Brighton C,McMahon DJ,Oberg CJ,Lefevre M,Broadbent JR,Steele JL

    更新日期:2014-08-01 00:00:00

  • Molecular characterization of isolates of Beauveria bassiana obtained from overwintering and summer populations of Sunn Pest (Eurygaster integriceps).

    abstract:AIM:To examine whether isolates of the entomopathogenic fungus Beauveria bassiana are more closely associated to their summer hosts compared with overwintering hosts, with recently developed molecular tools based on mitochondrial regions. METHODS AND RESULTS:Primers for the traditional ITS1-5.8S-ITS2 region and two mi...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02331.x

    authors: Kouvelis VN,Ghikas DV,Edgington S,Typas MA,Moore D

    更新日期:2008-03-01 00:00:00

  • Use of a putative transcriptional regulator gene as target for specific identification of Staphylococcus epidermidis.

    abstract:AIMS:To investigate the use of a Staphylococcus epidermidis transcriptional regulator gene as target for species-specific determination. METHODS AND RESULTS:Staph. epidermidis genes encoding putative transcriptional regulators were retrieved from GenBank and those showing no homology with other bacterial sequences wer...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.01948.x

    authors: Liu D,Swiatlo E,Austin FW,Lawrence ML

    更新日期:2006-09-01 00:00:00

  • Isolation and characterization of multidrug-resistant Leclercia species from animal clinical case.

    abstract::Leclercia adecarboxylata, a Gram-negative bacillus of family Enterobacteriaceae, is an uncommonly identified pathogen isolated from environmental and clinical specimens. Most of the human infections are polymicrobial and commonly occur in immunocompromised hosts, although nosocomial infections in immunocompetent hosts...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12811

    authors: Choudhary M,Choudhary BK,Bhoyar S,Kale SB,Chaudhari SP,Bera BC,Jain A,Barbuddhe SB

    更新日期:2018-01-01 00:00:00

  • Occurrence of fungi in water used at a haemodialysis centre.

    abstract:AIMS:The aim of this study was to identify and determine the diversity, occurrence and distribution of fungi in water used at a haemodialysis centre. METHODS AND RESULTS:Samples in the hydraulic circuit for the distribution of the water, dialysate samples and samples of sterilization solution from dialysers were colle...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02349.x

    authors: Pires-Gonçalves RH,Sartori FG,Montanari LB,Zaia JE,Melhem MS,Mendes-Giannini MJ,Martins CH

    更新日期:2008-05-01 00:00:00

  • A simple method for normalization of DNA extraction to improve the quantitative detection of soil-borne plant pathogenic oomycetes by real-time PCR.

    abstract:UNLABELLED:Most of the current research into the quantification of soil-borne pathogenic oomycetes lacks determination of DNA extraction efficiency, probably leading to an incorrect estimation of DNA quantity. In this study, we developed a convenient method by using a 100 bp artificially synthesized DNA sequence derive...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12441

    authors: Li M,Ishiguro Y,Kageyama K,Zhu Z

    更新日期:2015-08-01 00:00:00

  • Effect of hydrostatic pressure on the morphology and ultrastructure of wild-type and trehalose synthase mutant cells of Saccharomyces cerevisiae.

    abstract:AIMS:Saccharomyces cerevisiae was used for studying the physiological effects of hydrostatic pressure. METHODS AND RESULTS:The effects of hydrostatic pressure on the ultrastructure of wild-type and trehalose-6-phosphate synthase (tps1) mutant cells were investigated by transmission electron microscopy. Pressure induce...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2001.00853.x

    authors: Fernandes PM,Farina M,Kurtenbach E

    更新日期:2001-01-01 00:00:00

  • Re-interpretation of the logistic equation for batch microbial growth in relation to Monod kinetics.

    abstract:AIMS:To determine the underlying substrate utilization mechanism in the logistic equation for batch microbial growth by revealing the relationship between the logistic and Monod kinetics. Also, to determine the logistic rate constant in terms of Monod kinetic constants. METHODS AND RESULTS:The logistic equation used t...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02537.x

    authors: Kargi F

    更新日期:2009-04-01 00:00:00

  • Divercin V41 from gene characterization to food applications: 1998-2008, a decade of solved and unsolved questions.

    abstract::The emergence of an increasing number of antibiotic resistant human clinical bacteria has been a great cause of concern for the last decades. As an example, Staphylococcus aureus isolates in the hospital environment are becoming more and more resistant to antibiotics including vancomycin which is considered as a last ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1472-765X.2008.02490.x

    authors: Rihakova J,Belguesmia Y,Petit VW,Pilet MF,Prévost H,Dousset X,Drider D

    更新日期:2009-01-01 00:00:00