Abstract:
AIMS:To investigate the genetics of dimethoate degradation in Pseudomonas aeruginosa MCMB-427. METHODS AND RESULTS:Pseudomonas aeruginosa MCMB-427 demonstrated the ability to degrade dimethoate, a synthetic organophosphate insecticide. Total DNA preparation of MCMB-427 revealed the presence of a 6.6 kbp plasmid (designated as pDMD427). Escherichia coli NovaBlue transformed with plasmid pDMD427 subsequently acquired the ability to degrade dimethoate. Curing of the plasmid by plumbagin or ethidium bromide resulted in the loss of ability of MCMB-427 to degrade dimethoate. Plasmid pDMD427 was stable in MCMB-427 over 20 passages without selection. Genes encoding resistance to norfloxacin and cobalt were also located on plasmid pDMD427. CONCLUSION:The ability of Ps. aeruginosa MCMB-427 to degrade dimethoate is plasmid-mediated and transferable to other strains. SIGNIFICANCE AND IMPACT OF THE STUDY:As far as is known, this is the first report of plasmid-mediated dimethoate biodegradation. This study contributes significantly towards an understanding of the genetics of bacterial dimethoate degradation.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Deshpande NM,Dhakephalkar PK,Kanekar PPdoi
10.1046/j.1472-765x.2001.00995.xsubject
Has Abstractpub_date
2001-10-01 00:00:00pages
275-9issue
4eissn
0266-8254issn
1472-765Xpii
lam995journal_volume
33pub_type
杂志文章abstract:AIMS:This study was aimed to investigate the effectiveness of high-pressure homogenization (HPH) against Alicyclobacillus acidoterrestris. METHODS AND RESULTS:The susceptibility of three different strains of A. acidoterrestris (DSMZ 2498, Gamma4 and c8) to HPH (500-1700 bar) was studied. The experiments were performed...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02219.x
更新日期:2007-10-01 00:00:00
abstract::Hard-shelled mussel (Mytilus coruscus) is a popular seafood in Korea. This study aimed to determine the virulence markers and antimicrobial resistance patterns of 33 Aeromonas strains isolated from mussels. The isolates were identified as A. salmonicida (n = 14), A. veronii (n = 9), A. enteropelogenes (n = 4), A. cavi...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13266
更新日期:2020-03-01 00:00:00
abstract:AIMS:The aim of this study was to detect the main pathogenic human RNA enteric viruses able to persist in the environment such as astrovirus, enterovirus, norovirus and hepatitis A virus (HAV) in shellfish collected from two locations in northern Tunisia. METHODS AND RESULTS:Viruses were eluted from digestive tissues ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01978.x
更新日期:2006-10-01 00:00:00
abstract:AIMS:Aims of investigation: (i) develop a quantitative RT-PCR for noroviruses and (ii) evaluate it on environmental samples. METHODS AND RESULTS:Noroviruses in environmental water samples were concentrated by adsorption/elution/flocculation. Sewage was processed by clarification and protein flocculation. Norovirus-spe...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01534.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:Dynamic microbial characterization of the colonization of organic carrier during a model biofiltration experiment using methanol as air pollutant. METHODS AND RESULTS:A model biofilter was used in order to characterize the micro-organisms involved in the colonization of a model organic carrier. The model system c...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01530.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:The following polymers were developed: polyethylene (PE), a PE and polyethylene oxide (70% PE and 30% PEO; PE + PEO) blend, PE and nisin (PE + nisin), PE, nisin, and EDTA (PE + nisin + EDTA), and PE + PEO with nisin (PE + PEO + nisin). METHODS AND RESULTS:Of the polymers tested, PE and PE + PEO did not exhibit an...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.01005.x
更新日期:2001-10-01 00:00:00
abstract:AIMS:To isolate, characterize and identify lactic acid bacteria (LAB) in the vineyards where koshu grapes, a primary wine grape cultivar in Japan, are grown. METHODS AND RESULTS:Sixty samples, including leaves, undamaged grape berries and soil under damaged berries, were collected at four koshu vineyards in Yamanashi ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2008.02398.x
更新日期:2008-08-01 00:00:00
abstract:AIMS:To develop a novel method for rapid construction of Campylobacter jejuni deletion mutants. METHODS AND RESULTS:We used overlapping extension PCR protocol to amplify a target sequence region of Camp. jejuni genomic DNA in which an internal fragment, Cj0618 coding sequence, was replaced by a chloramphenicol resista...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02232.x
更新日期:2007-12-01 00:00:00
abstract:AIMS: To show that application of copper hydroxide citrus sprays mixed with field source water (possibly contaminated) will not support Escherichia coli on plant surfaces. Environmental stresses of transient phyllosphere bacteria and presence of copper will eradicate these bacteria before harvest. METHODS AND RESULTS...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03179.x
更新日期:2012-02-01 00:00:00
abstract:AIMS:To determine whether nonspore-forming biothreat agents can survive and persist in potable water that does not contain a disinfectant. METHODS AND RESULTS: Autoclaved, de-chlorinated Atlanta municipal water was inoculated with eight isolates of bacterial biothreat agents (10⁶ CFU ml⁻¹). The inoculated water sample...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2012.03277.x
更新日期:2012-09-01 00:00:00
abstract:AIMS:To evaluate the effect of oregano essential oil on Listeria monocytogenes cytoplasmic membrane. METHODS AND RESULTS:Nitroxide free-radical Electron Paramagnetic Resonance was applied on L. monocytogenes after 30 min exposure to oregano essential oil concentrations ranging from 0 to 1.25%. The impact of essential ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02877.x
更新日期:2010-08-01 00:00:00
abstract:UNLABELLED:Ligninolytic fungi take part in critical processes in ecosystems such as nutrient recycling; however, some fungal species can be pathogenic to forest and urban trees and deteriorate wood products. The tropical flora is an important source of antimicrobial compounds environmentally safer than traditional wood...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12159
更新日期:2014-01-01 00:00:00
abstract::The fate of Salmonella enteritidis PT4 in home-made mayonnaise prepared with citric acid solution (citric acid concentration of > or = 4.98% (w/v)) was investigated. It was found that pH of mayonnaise is closely related to the ratio of egg yolk to citric acid, and the inactivation rate of the micro-organisms increases...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00473.x
更新日期:1999-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12581
更新日期:2016-07-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01098.x
更新日期:2002-01-01 00:00:00
abstract::The pattern of DNA fragments produced by the polymerase chain reaction using arbitrary primers is strongly influenced by the age of the colonies sampled. We show that the number, clarity and reproducibility of the bands produced is optimal when very young colonies are sampled. ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1993.tb01467.x
更新日期:1993-12-01 00:00:00
abstract:AIMS:Strains of Clostridium butyricum have been increasingly used as probiotics for both animals and humans. The aim of this study was to develop a growth medium for cultivating C. butyricum ZJUCB using a statistical methodology. METHODS AND RESULTS:Response surface methodology (RSM) was used to evaluate the effects o...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01595.x
更新日期:2004-01-01 00:00:00
abstract:UNLABELLED:Newly synthesized erythromycin derivatives were screened for synergy with oxacillin and other β-lactam antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). MRSA ATCC43300 and some clinically isolated MRSA were used. Several erythromycin derivatives were found to possess high synergism with...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12378
更新日期:2015-04-01 00:00:00
abstract:UNLABELLED:Chrysanthemum plants are subject to serious virus diseases, so detection and identification of virus pathogens is important to prevent the virus spread. A reliable one-step multiplex RT-PCR was developed to simultaneously detect two viruses and two viriods: chrysanthemum virus B, tomato Aspermy virus, chrysa...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12007
更新日期:2013-01-01 00:00:00
abstract:AIMS:To simplify the electrotransformation process of Clostridium acetobutylicum, which currently needs to be performed in an anaerobic chamber, thus laborious and time-consuming. METHODS AND RESULTS:The CAC2634 gene encoding PerR is a known peroxide regulon repressor in Cl. acetobutylicum. CAC2634 in a previously con...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03111.x
更新日期:2011-09-01 00:00:00
abstract:AIMS:To examine changes in membrane fatty acid profile attributed to the physiological adaptation of Lactobacillus helveticus during vacuum drying. METHODS AND RESULTS:The viability and membrane integrity of the cells after vacuum drying were measured by plate counts and DNA fluorescence dyes. The physiological adapta...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02703.x
更新日期:2009-10-01 00:00:00
abstract:AIMS:Immunologically based assays for the detection of staphylococcal enterotoxins are numerous. These techniques include radio immunosorbent assays and enzyme-linked immunosorbent assays (ELISA), some of which are available as commercial kits. The purpose of this study was to compare the performances of three commerci...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01602.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:To study the cellular growth and morphology of Yarrowia lipolytica W29 and its lipase and protease production under increased air pressures. METHODS AND RESULTS:Batch cultures of the yeast were conducted in a pressurized bioreactor at 4 and 8 bar of air pressure and the cellular behaviour was compared with cultur...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02299.x
更新日期:2008-02-01 00:00:00
abstract:AIMS:Overproduced alpha-amylases in Bacillus subtilis provoke a specific stress response involving the CssRS two-component system, which controls expression of the HtrA and HtrB proteases. Previously, the B. subtilis TepA protein was implicated in high-level alpha-amylase secretion. Our present studies were aimed at in...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01539.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:The aim of the study was to investigate the antagonistic interactions of Trichoderma species against Rhizoctonia solani sclerotia by enhanced green fluorescence protein (EGFP)-tagged transformant of Trichoderma virens TY009. METHODS AND RESULTS:An EGFP was used as a report gene for transforming T. virens strain, ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02737.x
更新日期:2009-12-01 00:00:00
abstract:AIMS:The purpose of this study was to develop a loop-mediated isothermal amplification (LAMP) method for the rapid, sensitive and simple detection of Vibrio alginolyticus in mariculture fish. METHODS AND RESULTS:LAMP primers were designed by targeting the gyrB gene. With Bst DNA polymerase, the target DNA can be clear...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02823.x
更新日期:2010-05-01 00:00:00
abstract::Increasingly, molecular methods have become important in identification and confirmation of bacteria at the species level. Rapid molecular methods provide sensitivity and specificity while reducing cost and resources. The primary goal of this study was to develop a real-time PCR assay for identification of Escherichia...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12359
更新日期:2015-02-01 00:00:00
abstract::The growth of the oxidatively imperfect yeast Candida maltosa Komagata, Nakase et Katsuya was studied experimentally and modelled mathematically in relation to sodium benzoate and lactic acid concentrations at different temperatures. Application of gamma models for the growth rate resulted in determination of cardinal...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12803
更新日期:2017-11-01 00:00:00
abstract:AIMS:To establish species-specific PCR and DNA probe methods for Streptococcus salivarius and to clarify the distribution of dextranase in oral isolates of Strep. salivarius. METHODS AND RESULTS:A pair of PCR primers and a DNA probe were designed based on the nucleotide sequence of the dextranase gene of Strep. saliva...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.00928.x
更新日期:2001-06-01 00:00:00
abstract::Quantitative and qualitative changes in organic matter content of wastewater effluents attributable to chlorination and ozonation have been analysed using bioassays as well as organic carbon direct measures. Bioassays were carried out using the bacterial populations of wastewater and two Escherichia coli strains as te...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.00793.x
更新日期:2000-08-01 00:00:00