Lecanicillium aphanocladii: snake venom phospholipases A2 and proteases as tools to prospect enzymatic inhibitors.

Abstract:

:Natural enzyme inhibitors have been widely described in literature because of its pharmacological and cosmetic applications. Fungi found in caves represent a promising source of bioactive substances that are still little explored scientifically. Thus, the present work evaluated the presence of enzymatic modulators in a filtrate obtained from the cultivation of the cave fungus Lecanicillium aphanocladii (Family: Cordycipitaceae). Snake venoms from Bothrops alternatus and Bothrops atrox were used as an enzymatic source for the induction of the phospholipase, proteolytic, thrombolytic, cytotoxic and coagulant activities. Compounds present in the fungal filtrate inhibited 50, 23·8, 26·6, 50·9 and 52·5% of the proteolytic, phospholipase, haemolytic, thrombolytic and coagulant activities respectively. The filtrate was not cytotoxic on erythrocytes, but induced partial dissolution of thrombi. Fungal enzyme inhibitors that have low or no toxicity and can be obtained on a large scale and at low cost are relevant in the medical-scientific context. Therefore, the inhibition of phospholipases A2 and proteases observed in the present work highlights the potential of fungal metabolites for the development of drugs that can be used in the treatment of haemostasis and inflammation-related disorders. SIGNIFICANCE AND IMPACT OF THE STUDY: In this study, secondary metabolites synthesized by Lecanicillium aphanocladii, a fungus isolated from caves, demonstrated modulating action on proteases and phospholipases A2 present in snake venoms of the Bothrops genus, widely used as tools for the study of pathophysiology processes related to haemostasis and inflammation. The results suggest the possibility of future applications for these metabolites in the development of pharmaceuticals of medical-scientific interest.

journal_name

Lett Appl Microbiol

authors

Cardoso MGB,Trento MVC,Reis CH,Marcussi S,Cardoso PG

doi

10.1111/lam.13171

subject

Has Abstract

pub_date

2019-08-01 00:00:00

pages

88-95

issue

2

eissn

0266-8254

issn

1472-765X

journal_volume

69

pub_type

杂志文章
  • Studies of the release of small molecules during pressure germination of spores of Bacillus subtilis.

    abstract:AIMS:To measure rates of release of small molecules during pressure germination of Bacillus subtilis spores, and the role of SpoVA proteins in dipicolinic acid (DPA) release. METHODS AND RESULTS:Rates of DPA release during B. subtilis spore germination with pressures of 150 or 500 megaPascals were much higher in spore...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02204.x

    authors: Vepachedu VR,Hirneisen K,Hoover DG,Setlow P

    更新日期:2007-09-01 00:00:00

  • Enhanced heat stable antifungal factor production by Lysobacter enzymogenes OH11 with cheap feedstocks: medium optimization and quantitative determination.

    abstract::Heat stable antifungal factor (HSAF) is considered to be a potential biological pesticide due to its broad antifungal activity and novel mode of action. However, few studies have reported on HSAF production during fermentation. Thus, this work was executed to optimize the medium composition to maximize HSAF production...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12870

    authors: Tang B,Zhao YC,Shi XM,Xu HY,Zhao YY,Dai CC,Liu FQ

    更新日期:2018-05-01 00:00:00

  • Blue light (470 nm) effectively inhibits bacterial and fungal growth.

    abstract:UNLABELLED:Blue light (470 nm) LED antimicrobial properties were studied alone against bacteria and with or without the food grade photosensitizer, erythrosine (ERY) against filamentous fungi. Leuconostoc mesenteroides (LM), Bacillus atrophaeus (BA) or Pseudomonas aeruginosa (PA) aliquots were exposed on nutrient agar ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12002

    authors: De Lucca AJ,Carter-Wientjes C,Williams KA,Bhatnagar D

    更新日期:2012-12-01 00:00:00

  • Deciphering the DNA repair protein, Rad23 from kuruma shrimp Marsupenaeus japonicus: full-length cDNA cloning and characterization.

    abstract:AIMS: Lesions of DNA are removed by nucleotide excision repair (NER) process in the living systems. NER process-related host factors are believed to aid recovery steps during viral integration. Here, we report identification and characterization of a DNA repair molecule Rad23 from kuruma shrimp Marsupenaeus japonicus. ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2011.03073.x

    authors: Sudhakaran R,Okugawa S,Mekata T,Inada M,Yoshimine M,Nishi J,Ozono C,Kono T,Sakai M,Itami T

    更新日期:2011-07-01 00:00:00

  • Identification and removal of contaminating microbial DNA from PCR reagents: impact on low-biomass microbiome analyses.

    abstract::Reagent-derived contamination can compromise the integrity of microbiome data, particularly in low microbial biomass samples. This contamination has recently been attributed to the 'kitome' (contamination introduced by the DNA extraction kit), prior to which attention was mostly paid to potential contamination introdu...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13091

    authors: Stinson LF,Keelan JA,Payne MS

    更新日期:2019-01-01 00:00:00

  • New media for detection and counting of Clostridia in foods.

    abstract::The growth of pure cultures of Clostridium perfringens (ATCC 12922) and Cl. sporogenes (PA 3679) in five non-selective media, fluid thioglycollate medium (FTM), rapid perfringens medium (RPM), Columbia broth Malthus (CBM), reinforced clostridial medium (RCM) and lactose sulphite (LS), was monitored using conductance m...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1997.00330.x

    authors: Dromigny E,Bourrion F,Rugraf Y,Bolton FJ,Leden N

    更新日期:1997-01-01 00:00:00

  • Viability of zoonotic pathogens Escherichia coli and Salmonella in swine manure slurries with and without a urease inhibitor and thymol.

    abstract:AIMS:To determine the effects of urine, a urease inhibitor and/or an odour-reducing antimicrobial compound, on zoonotic pathogens in swine manure slurries. METHODS AND RESULTS:Swine faeces were collected and blended with different amounts of urine. Marker strains of Salmonella enterica subsp. enterica serovar Typhimur...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02338.x

    authors: Wells JE,Varel VH

    更新日期:2008-04-01 00:00:00

  • Evaluation of the diversity of Paenibacillus polymyxa strains by using the DNA of bacteriophage IPy1 as a probe in hybridization experiments.

    abstract:AIMS:To evaluate the genetic diversity within the species Paenibacillus polymyxa. METHODS:Southern hybridization was performed on 102 strains of P. polymyxa using DNA from the phage IPy1 as a probe. RESULTS:All 102 strains hybridized to phage IPy1 DNA. Data from different hybridization patterns obtained were used to ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01132.x

    authors: Santos SC,Rodrigues Coelho MR,Seldin L

    更新日期:2002-01-01 00:00:00

  • Rumen microbial population dynamics in response to photoperiod.

    abstract:AIMS:This work was carried out to determine if there was a difference in the microbial population of the rumen associated with daylength at which sheep are housed. METHODS AND RESULTS:Denaturing gradient gel electrophoresis (DGGE) was used to study the ciliate and bacterial diversity in the rumen of Soay rams kept in ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2005.01707.x

    authors: McEwan NR,Abecia L,Regensbogenova M,Adam CL,Findlay PA,Newbold CJ

    更新日期:2005-01-01 00:00:00

  • Genetic heterogeneity of non-O1 and non-O139 Vibrio cholerae isolates from shrimp aquaculture system: a comparison of RS-, REP- and ERIC-PCR fingerprinting approaches.

    abstract:AIMS:The genetic diversity of Vibrio cholerae isolated from black tiger shrimp (Penaeus monodon) aquaculture farms was determined using three PCR typing methods based on enterobacterial repetitive intergenic consensus (ERIC) sequences, ribosomal gene spacer (RS) sequence and repetitive extragenic palindromic (REP) sequ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2010.02863.x

    authors: Rao BM,Surendran PK

    更新日期:2010-07-01 00:00:00

  • Specific molecular detection of Carnobacterium piscicola SF668 in cold smoked salmon.

    abstract:AIMS:To establish a rapid and reliable multiplex PCR (mPCR)-based method allowing specific identification of Carnobacterium piscicola SF668 during storage of cold smoked salmon (CSS). METHODS AND RESULTS:CSS was inoculated with C. piscicola SF668 and stored at 4 degrees C. Samples were withdrawn at regular time interv...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2005.01696.x

    authors: Pellé E,Dousset X,Prévost H,Drider D

    更新日期:2005-01-01 00:00:00

  • Environmental factors influencing the inactivation of Listeria monocytogenes by pulsed electric fields.

    abstract:AIMS:To investigate the influence of the growth phase, growth temperature, storage time, pH and aw of the treatment medium on the resistance of Listeria monocytogenes to pulsed electric fields (PEF). METHODS AND RESULTS:Square wave pulses of 2 micros at a frequency of 1 Hz and 25 and 28 kV cm(-1) were used. Cells were...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01221.x

    authors: Alvarez I,Pagán R,Raso J,Condón S

    更新日期:2002-01-01 00:00:00

  • Adhesion capability of first two domains at N terminus of NP_785232 protein and their interaction with a UV-absorbing component from human mucus.

    abstract:AIMS:This work aims to investigate the binding capability of certain domains at N terminus of the protein NP_785232 of Lactobacillus plantarum to Caco-2 cells and to test the usage of affinity chromatography to isolate the human mucus component that interacts with them. METHODS AND RESULTS:Recombinant proteins contain...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2010.02911.x

    authors: Du L,Liu F,Ju X,Huo G

    更新日期:2010-10-01 00:00:00

  • Control of biofouling by xanthine oxidase on seawater reverse osmosis membranes from a desalination plant: enzyme production and screening of bacterial isolates from the full-scale plant.

    abstract::Control of biofouling on seawater reverse osmosis (SWRO) membranes is a major challenge as treatments can be expensive, damage the membrane material and often biocides do not remove the polymers in which bacteria are embedded. Biological control has been largely ignored for biofouling control. The objective of this st...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12747

    authors: Nagaraj V,Skillman L,Li D,Xie Z,Ho G

    更新日期:2017-07-01 00:00:00

  • Erythritol production by Moniliella megachiliensis using nonrefined glycerol waste as carbon source.

    abstract:UNLABELLED:The number of naphtha plants is being reduced due to a worldwide shift in energy sources. Consequently, a shortage of chemical materials heavily dependent on naphtha-oil, especially C4 compounds such as butene and butane-diol, is an urgent issue in chemical manufacturing. Erythritol is a rare C4 compound pro...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12391

    authors: Kobayashi Y,Iwata H,Mizushima D,Ogihara J,Kasumi T

    更新日期:2015-05-01 00:00:00

  • A capillary polymerase chain reaction for Salmonella detection from poultry meat.

    abstract:AIMS:In this study, a capillary polymerase chain reaction (cPCR) was applied for Salmonella detection from poultry meat. METHODS AND RESULTS:Salmonella detection limits of the optimized cPCR were determined with DNA templates from the samples of tetrathionate broth (TTB), Rappaport Vassiliadis broth (RVB) and selenite...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.02033.x

    authors: Gunaydin E,Eyigor A,Carli KT

    更新日期:2007-01-01 00:00:00

  • Isolation of endophytic diazotroph Pantoea agglomerans and nondiazotroph Enterobacter asburiae from sweetpotato stem in Japan.

    abstract:AIMS:To isolate and identify diazotrophic endophytes in the stem of Japanese sweetpotato cv. Koganesengan. METHODS AND RESULTS:Surface-sterilized and thinly sliced (1-2 mm) sweetpotato stem samples were incubated in test tubes with semi-solid modified Rennie (MR) medium. The test tubes were assayed for acetylene reduc...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01434.x

    authors: Asis CA Jr,Adachi K

    更新日期:2004-01-01 00:00:00

  • Antibacterial activity of chemotherapeutic drugs against Escherichia coli and Staphylococcus pseudintermedius.

    abstract::The ability of chemotherapeutic agents to affect the growth of common bacterial pathogens and the relationship between the effects of chemotherapeutics and antimicrobials is largely unknown. The purpose of this study was to describe the susceptibility of canine bacterial isolates to chemotherapeutic agents and to comp...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13213

    authors: Campbell O,Gagnon J,Rubin JE

    更新日期:2019-11-01 00:00:00

  • Design of a species-specific PCR method for the detection of the heat-resistant fungi Talaromyces macrosporus and Talaromyces trachyspermus.

    abstract::Heat-resistant fungi occur sporadically and are a continuing problem for the food and beverage industry. The genus Talaromyces, as a typical fungus, is capable of producing the heat-resistant ascospores responsible for the spoilage of processed food products. Isocitrate lyase, a signature enzyme of the glyoxylate cycl...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12818

    authors: Yamashita S,Nakagawa H,Sakaguchi T,Arima TH,Kikoku Y

    更新日期:2018-01-01 00:00:00

  • Identification of Streptococcus salivarius by PCR and DNA probe.

    abstract:AIMS:To establish species-specific PCR and DNA probe methods for Streptococcus salivarius and to clarify the distribution of dextranase in oral isolates of Strep. salivarius. METHODS AND RESULTS:A pair of PCR primers and a DNA probe were designed based on the nucleotide sequence of the dextranase gene of Strep. saliva...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2001.00928.x

    authors: Igarashi T,Yano Y,Yamamoto A,Sasa R,Goto N

    更新日期:2001-06-01 00:00:00

  • Lead-resistant Providencia alcalifaciens strain 2EA bioprecipitates Pb+2 as lead phosphate.

    abstract::A lead-resistant bacteria isolated from soil contaminated with car battery waste were identified as Providencia alcalifaciens based on biochemical characteristics, FAME profile and 16S rRNA sequencing and designated as strain 2EA. It resists lead nitrate up to 0·0014 mol l(-1) by precipitating soluble lead as insolubl...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12026

    authors: Naik MM,Khanolkar D,Dubey SK

    更新日期:2013-02-01 00:00:00

  • Characterization of Bacillus thuringiensis serovar bolivia (serotype H63), a novel serovar isolated from the Bolivian high valleys.

    abstract::The type strain Bacillus thuringiensis var. bolivia (serotype H63), isolated from the Bolivian high valleys, has been characterized at different levels. Its parasporal crystal has an unusual shape and it is composed of a protein of 155 kDa which shows two bands of 75 and 80 kDa after activation. Analysis by PCR shows ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00558.x

    authors: Ferrandis MD,Andrew R,Porcar M,Iriarte J,Cosmao-Dumanoir V,Lecadet MM,Caballero P,Ferré J

    更新日期:1999-06-01 00:00:00

  • Airborne enteric micro-organisms and ammonia levels in diaper-changing rooms in kindergartens.

    abstract:AIMS:  We evaluated risks associated with diaper changing in Finnish kindergartens where children were using either modern disposable paper or reusable cloth diapers. METHODS AND RESULTS:  We determined enteric micro-organisms and ammonia in diaper-changing rooms in four kindergartens in autumn and winter in the ambie...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2012.03230.x

    authors: Vaattovaara PE,Kivimäenpää M,Vaattovaara P,Pasanen P,Heinonen-Tanski H

    更新日期:2012-05-01 00:00:00

  • Comparison of bacteriostatic and bactericidal activity of 13 essential oils against strains with varying sensitivity to antibiotics.

    abstract:AIMS:To compare the bacteriostatic and bactericidal activity of 13 chemotyped essential oils (EO) on 65 bacteria with varying sensitivity to antibiotics. METHODS AND RESULTS:Fifty-five bacterial strains were tested with two methods used for evaluation of antimicrobial activity (CLSI recommendations): the agar dilution...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02406.x

    authors: Mayaud L,Carricajo A,Zhiri A,Aubert G

    更新日期:2008-09-01 00:00:00

  • Effect of a sublethal high-pressure homogenization treatment on the fatty acid membrane composition of probiotic lactobacilli.

    abstract:UNLABELLED:High-pressure homogenization (HPH) has been proposed to be applied directly to lactic acid bacterial cells at sublethal levels to enhance some functional properties. As the principal target of HPH are the cell surface envelope structures, the aim of this work was to study the effect of a HPH treatment, appli...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12164

    authors: Tabanelli G,Patrignani F,Gardini F,Vinderola G,Reinheimer J,Grazia L,Lanciotti R

    更新日期:2014-02-01 00:00:00

  • A heat inducible tyrosinase with distinct properties from Bacillus thuringiensis.

    abstract:AIMS:The objective of this work was to purify the tyrosinase from Bacillusthuringiensis subsp. kurstaki (Bt) (CCTCC AB 90010) and study its enzymatic properties. METHODS AND RESULTS:A 'one-step' purification method was used in this work, which was an easy, high-yield purification method. Tyrosinase activity of this pu...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2004.01599.x

    authors: Liu N,Zhang T,Wang YJ,Huang YP,Ou JH,Shen P

    更新日期:2004-01-01 00:00:00

  • Diversity of bacterial capabilities in utilizing alkylated benzenes and other aromatic compounds.

    abstract::Seventeen aerobic bacterial strains known to degrade one or more aromatic compounds were tested for their ability to utilize benzene, seven different methylated benzenes or other aromatic compounds as their sole source of carbon. The results are discussed with respect to the possibility of finding strains which degrad...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1996.tb00078.x

    authors: Lang E

    更新日期:1996-10-01 00:00:00

  • Two-dimensional environmental profiles of growth, deoxynivalenol and nivalenol production by Fusarium culmorum on a wheat-based substrate.

    abstract:AIMS:To determine the effect of interacting conditions of water activity (aw, 0.99-0.85), temperature (15, 25 degrees C) and time (40 days) on growth and production of the mycotoxins deoxynivalenol (DON) and nivalenol (NIV) by Fusarium culmorum on a wheat-based agar medium. METHODS AND RESULTS:Fusarium culmorum grew o...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01358.x

    authors: Hope R,Magan N

    更新日期:2003-01-01 00:00:00

  • Molecular characterization of isolates of Beauveria bassiana obtained from overwintering and summer populations of Sunn Pest (Eurygaster integriceps).

    abstract:AIM:To examine whether isolates of the entomopathogenic fungus Beauveria bassiana are more closely associated to their summer hosts compared with overwintering hosts, with recently developed molecular tools based on mitochondrial regions. METHODS AND RESULTS:Primers for the traditional ITS1-5.8S-ITS2 region and two mi...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02331.x

    authors: Kouvelis VN,Ghikas DV,Edgington S,Typas MA,Moore D

    更新日期:2008-03-01 00:00:00

  • The prevalence of opportunistic pathogens associated with intraoral implants.

    abstract:AIMS:The aim of this study was to determine the prevalence and proportions of opportunistic pathogens harboured on orthodontic retainers. METHODS AND RESULTS:First, Staphylococcus spp. and Candida spp. were isolated from the retainer's inner surface and from other mucosal surfaces of the subject's mouth by routine bac...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2011.03031.x

    authors: Al Groosh D,Roudsari GB,Moles DR,Ready D,Noar JH,Pratten J

    更新日期:2011-05-01 00:00:00